RESUMO
Japanese encephalitis (JE) virus replicated in monkey, pig and day-old chick leucocyte cultures. The titres obtained on days 3 to 5 after infection in monkey, pig and chick leucocyte cultures were comparable. Treatment of monkey leucocyte cultures with the mitogens phytohaemagglutinin P, pokeweed mitogen (PWM), formalinized Staphylococcus aureus (Cowan I) or concanavalin A and pig leucocytes with PWM did not significantly affect their ability to support replication of JE virus. No relationship was observed between the amount of [3H]thymidine incorporated in untreated or mitogen treated monkey or pig leucocyte cultures and the titres of JE virus in such cultures. The ability of monkey, pig and chick leucocyte cultures to support JE virus replication was abrogated following silica treatment. These findings suggest that monocytes may serve as one of the important sites of JE virus replication.
Assuntos
Vírus da Encefalite Japonesa (Espécie)/fisiologia , Replicação Viral , Animais , Transformação Celular Viral/efeitos dos fármacos , Galinhas , Leucócitos/efeitos dos fármacos , Leucócitos/fisiologia , Macaca radiata , Mitógenos/farmacologia , SuínosRESUMO
A comparative study of interferon (IFN) production (type-alpha and gamma) was carried out using Ficoll-hypaque purified fresh and cryopreserved mononuclear cells from eight normal healthy individuals. Newcastle disease virus-NDV (R2B strain) was used as an inducer for type-alpha and Staphylococcal enterotoxin-A-(SEA) for type-gamma IFN production. There was no significant difference between the titres of type-alpha and gamma-IFN and lymphocyte subpopulations of fresh and cryopreserved mononuclear cells studied under identical conditions.