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1.
Environ Microbiol ; 22(12): 5137-5155, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-32524747

RESUMO

Pseudomonas isolates from tropical environments have been underexplored and may form an untapped reservoir of interesting secondary metabolites. In this study, we compared Pseudomonas and cyclic lipopeptide (CLP) diversity in the rhizosphere of a cocoyam root rot disease (CRRD) suppressive soil in Boteva, Cameroon with those from four conducive soils in Cameroon and Nigeria. Compared with other soils, Boteva andosols were characterized by high silt, organic matter, nitrogen and calcium. Besides, the cocoyam rhizosphere at Boteva was characterized by strains belonging mainly to the P. koreensis and P. putida (sub)groups, with representations in the P. fluorescens, P. chlororaphis, P. jessenii and P. asplenii (sub)groups. In contrast, P. putida isolates were prominent in conducive soils. Regarding CLP diversity, Boteva was characterized by strains producing 11 different CLP types with cocoyamide A producers, belonging to the P. koreensis group, being the most abundant. However, putisolvin III-V producers were the most dominant in the rhizosphere of conducive soils in both Cameroon and Nigeria. Furthermore, we elucidated the chemical structure of putisolvin derivatives-putisolvin III-V, and described its biosynthetic gene cluster. We show that high Pseudomonas and metabolic diversity may be driven by microbial competition, which likely contributes to soil suppressiveness to CRRD.


Assuntos
Lipopeptídeos/metabolismo , Peptídeos Cíclicos/metabolismo , Pseudomonas/isolamento & purificação , Microbiologia do Solo , Xanthosoma/microbiologia , Camarões , Lipopeptídeos/química , Nigéria , Peptídeos Cíclicos/química , Doenças das Plantas/microbiologia , Doenças das Plantas/prevenção & controle , Raízes de Plantas/microbiologia , Pseudomonas/classificação , Pseudomonas/metabolismo , Pythium/patogenicidade , Rizosfera , Solo/química
2.
J Environ Manage ; 203(Pt 1): 429-438, 2017 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-28834776

RESUMO

Historic long-term P application to sandy soils in NW-Europe has resulted in abundant sorption, saturation and eventually leaching of P from soil to the groundwater. Although many studies recognize the control of site-specific factors like soil texture and phosphate saturation degree (PSD), the regional-scaled relevance of effects exerted by single factors controlling P leaching is unclear. Very large observational datasets of soil and groundwater P content are furthermore required to reveal indirect controls of soil traits through mediating soil variables. We explored co-variation of phreatic groundwater orthophosphate (o-P) concentration and soil factors in sandy soils in Flanders, Belgium. Correlation analyses were complemented with an exploratory model derived using 'path analysis'. Data of oxalate-extractable Al, Fe, P and pHKCl, phosphate sorption capacity (PSC) and PSD in three depth layers (0-30, 30-60, 60-90 cm), topsoil SOC, % clay and groundwater depth (fluctuation) were interpolated to predict soil properties on exact locations of a very extensive net of groundwater monitoring wells. The mean PSD was only poorly correlated to groundwater o-P concentration, indicating the overriding control of other factors in the transport of P to the groundwater. A significant (P < 0.01) positive non-linear relationship was found between groundwater o-P concentrations and pHKCl for all depth layers. Likewise, lower SOC% (P < 0.01) and shallower groundwater level (MHL or MLL) corresponded (P < 0.01) with higher o-P concentrations. Groundwater o-P unexpectedly correlated positively to clay% and path analysis indicated this to be an indirect effect of the groundwater level. Path analysis furthermore indicated an important indirect control of pH on groundwater o-P concentrations and a considerable direct effect of Pox, 0-90, Alox, 0-90 and MHL. The fact that groundwater o-P concentration was stronger controlled by soil pH and groundwater table depth than by PSD indicates the likely oversimplification of the latter index to measure the long-term potential risk of P leaching. Accounting for controls on leaching not included in PSD via an alternative index, however, seems problematic as in Flanders for example groundwater o-P turned out to be higher in finer textured soils or soils with higher pedogenic Fe content, probably because of their lower pedogenic Al content and higher soil pH. Path analysis of extensive soil and groundwater datasets seems a viable way to identify prime local determinants of soil P leaching and could be further on used for 'ground-truthing' more complex P-migration simulation models.


Assuntos
Água Subterrânea , Fósforo , Poluentes Químicos da Água , Bélgica , Europa (Continente) , Fosfatos , Solo , Poluentes do Solo
3.
Sci Total Environ ; 899: 165749, 2023 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-37495131

RESUMO

Soil texture plays a crucial role in organic matter (OM) mineralization through both direct interactions with minerals and indirect effects on soil moisture. Separating these effects could enhance the modelling of soil organic carbon (SOC) dynamics under climate change scenarios. However, the attempts have been limited small-scale experiments. Here, we studied the effects of soil texture on added OM mineralization in loamy sand, loam and silt loam soils in nine agricultural fields in Flanders, Belgium. Soil moisture, temperature, groundwater table depth and the mineralization of 13C-labeled ryegrass were monitored in buried mesocosms for approximately three months during a dry summer. Ryegrass-C mineralization was lowest in the loamy sand (39 ± 7 %) followed by silt loam (48 ± 7 %) and loam (63 ± 5 %) soils, challenging the current clay%-based moderation of C-mineralization rates in soil models. Soil temperature was not influenced by soil texture, whereas soil moisture was indeed dependent on soil texture. It appears that capillarity sustained upward water supply from groundwater to the topsoil in loam and silt loam soils but not in loamy sand soil, although this difference in capillary rise could not fully explain the higher moisture content in loam than that in silt loam soils. Additionally, soil texture only impacted remnant added ryegrass pieces (>500 µm) but not the finer ryegrass-derived SOC (<500 µm), which might point at the important indirect control of texture on OM mineralization during prolonged summer drought. However, these effects are only manifested during drought when no other factors (e.g., groundwater depth or subsurface water flows) exert an overriding impact on the soil water balance. Overall, our findings highlight the need to properly incorporate the indirect effects of soil texture on OM mineralization into soil carbon models to accurately predict soil C stocks under future climate change scenarios.

4.
Oecologia ; 169(1): 221-34, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22120703

RESUMO

Fertilisation of agricultural land causes an accumulation of nutrients in the top soil layer, among which phosphorus (P) is particularly persistent. Changing land use from farmland to forest affects soil properties, but changes in P pools have rarely been studied despite their importance to forest ecosystem development. Here, we describe the redistributions of the P pools in a four-decadal chronosequence of post-agricultural common oak (Quercus robur L.) forests in Belgium and Denmark. The aim was to assess whether forest age causes a repartitioning of P throughout the various soil P pools (labile P, slowly cycling P and occluded P); in particular, we addressed the time-related alterations in the inorganic versus organic P fractions. In less than 40 years of oak forest development, significant redistributions have occurred between different P fractions. While both the labile and the slowly cycling inorganic P fractions significantly decreased with forest age, the organic fractions significantly increased. The labile P pool (inorganic + organic), which is considered to be the pool of P most likely to contribute to plant-available P, significantly decreased with forest age (from >20 to <10% of total P), except in the 0-5 cm of topsoil, where labile P remained persistently high. The shift from inorganic to organic P and the shifts between the different inorganic P fractions are driven by biological processes and also by physicochemical changes related to forest development. It is concluded that the organic labile P fraction, which is readily mineralisable, should be taken into account when studying the bioavailable P pool in forest ecosystems.


Assuntos
Fósforo/análise , Quercus/metabolismo , Solo/química , Bélgica , Carbono/análise , Carbono/metabolismo , Dinamarca , Concentração de Íons de Hidrogênio , Fósforo/metabolismo , Raízes de Plantas , Fatores de Tempo , Árvores/metabolismo
5.
J Contam Hydrol ; 241: 103820, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-33979710

RESUMO

Diffuse groundwater contamination by intense fertilizer use is a widespread problem in most of the agricultural regions in West-Europe and in many other countries worldwide. As a result elevated nitrate concentrations in groundwater are found, and as groundwater is the source of baseflow into rivers and streams, also surface waters show increased nitrate levels. Flanders in the north of Belgium is no exception and despite restrictions on the fertilizer use and remediation practices initiated in the mid-1990s, and still ongoing, in many stream monitoring points, peak concentrations are measured above the 50 mg/l criterium. In many monitoring stations there is apparently a long delay between the start of the restrictions and effect on water quality. Often this delay is related to high concentrations in the groundwater inflow and long residence and travel times of groundwater feeding the streams. To better understand this behavior and estimate response times a tool was developed that incorporates the main water flows in a typical catchment and considers the effects of varying groundwater ages. This tool is conceived as a parsimonious lumped parameter model, simple and easy enough to be used by many people but still capturing the main mechanisms end processes. In this paper the tool is explained and it is applied on two testcases in Flanders to show its performance.


Assuntos
Água Subterrânea , Poluentes Químicos da Água , Distribuição por Idade , Monitoramento Ambiental , Humanos , Nitratos/análise , Tempo de Reação , Rios , Poluentes Químicos da Água/análise
6.
Sci Rep ; 11(1): 370, 2021 01 11.
Artigo em Inglês | MEDLINE | ID: mdl-33432019

RESUMO

Degradability of organic matter (OM) in soil depends on its spatial location in the soil matrix. A recent breakthrough in 3D-localization of OM combined dual-energy X-ray CT-scanning with OsO4 staining of OM. The necessity for synchrotron-based µCT and the use of highly toxic OsO4 severely limit applications in soil biological experiments. Here, we evaluated the potential of alternative staining agents (silver nitrate, phosphomolybdenic acid (PMA), lead nitrate, lead acetate) to selectively enhance X-ray attenuation and contrast of OM in CT volumes of soils containing specific mineral soil particle fractions, obtained via lab-based X-ray µCT. In comparison with OsO4, administration of Ag+ and Pb2+ resulted in insufficient contrast enhancement of OM versus fine silt (< 20 µm) or clay (< 2 µm) mineral particles. The perfusion procedure used in this work induced changes in soil structure. In contrast, PMA staining resulted in a selective increase of OM's attenuation contrast, which was comparable to OsO4. However, OM discrimination from other soil phases remained a challenge. Further development of segmentation algorithms accounting for grey value patterns and shape of stained particulate OM may enable its automated identification. If successful in undisturbed soils, PMA staining may form an alternative to OsO4 in non-synchrotron based POM detection.

7.
Front Plant Sci ; 11: 520834, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33224155

RESUMO

As soil and soilless culture systems are highly dynamic environments, the structure of rhizosphere microbial communities is consistently adapting. There is a knowledge gap between the microbial community structure of soil based and soilless culture systems and thus we aimed at surveying their impact on diversity and composition of bacterial communities across a 10-month period in a tomato cultivation system. We compared community metrics between an soil based culture system fertilized with malt sprouts and blood meal, known for its slow and high mineralization rate, respectively and a soilless culture system fertilized with fish effluent or supplemented with an liquid organic fertilizer. Bacterial and fungal community composition was followed over time using two complementary techniques, phospholipid fatty acid analysis and 16S rRNA amplicon sequencing. Nitrogen dynamics and plant performance were assessed to provide insight on how bacterial diversity of soil and soilless microbial communities ultimately impacts productivity. Similar plant performance was observed in soilless culture systems and soil based system and yield was the highest with the aquaponics-derived fertilizer. Soil and soilless cultivating systems supplemented with different nitrogen-rich fertilizers differed on its characteristics throughout the experimental period. Fast-paced fluctuations in pH(H2O) and nutrient cycling processes were observed in growing medium. Physicochemical characteristics changed over time and interacted with bacterial community metrics. Multivariate analysis showed that plant length, pH, Flavisolibacter, phosphorus, chloride, ammonium, potassium, calcium, magnesium, sodium, electrical conductivity, nitrate, sulfate, and the bacterial genera Desulfotomaculum, Solirubrobacter, Dehalococcoides, Bythopirellula, Steroidobacter, Litorilinea, Nonomuraea were the most significant factors discriminating between natural soils supplemented with animal and plant by-products. Long-term fertilizer regimes significantly changed the PLFA fingerprints in both the soilless culture and soil based culture system. The use of these by-products in the soil was positively associated with arbuscular mycorrhizal fungi (AMF), which may influence rhizosphere communities through root exudates and C translocation. Community structure was distinct and consistently different over time, despite the fertilizer supplementation. The fungal microbial community composition was less affected by pH, while the composition of the bacterial communities (Actinomycetes, Gram-negative bacteria, and Gram-positive bacteria) was closely defined by soil pH, demonstrating the significance of pH as driver of bacterial community composition. Fertilizer application may be responsible for variations over time in the ecosystem. Knowledge about the microbial interactions in tomato cultivating systems opens a window of opportunity for designing targeted fertilizers supporting sustainable crop production.

8.
Water Res ; 141: 329-339, 2018 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-29804019

RESUMO

In north-west Europe, agricultural diffuse P losses are a major cause of eutrophication problems in surface waters. Given that the Water Framework Directive (WFD) demands fast water quality improvements and most of the actual P mitigation strategies tend to work on the long run, new short-term mitigation measures are urgently needed. We here report on the entire process of developing small scale field filters to remove P at the end of tile drains, starting from the screening of potential P sorbing materials (PSM): iron coated sand (ICS), acid pre-treated natural minerals (biotite, glauconite and olivine) and bauxite. Initial batch (ad)sorption experiments revealed following order in both, P sorption capacity and speed: ICS > bauxite > glauconite > olivine = biotite. Because of the presence of significant amounts of lead and/or nickel, we excluded bauxite and olivine from further experiments. Subsequent lab scale flow through systems were conducted with P filters containing mixtures of ICS and glauconite (100/0, 90/10, 80/20, 70/30 and 60/40%, respectively, on weight basis). We found a significant relationship between Ksat and the filter mixtures particle size distribution and bulk density, and a significant effect of the filter mixture composition on P removal efficiency and stability of Ksat. During the 10 week field trials, the pure ICS filters were capable of processing all drainage discharge rates (up to 6 m3 day-1) with a P removal efficiency of ≥74%. The 90/10 ICS/glauconite filters could process up to 4 m3 water day-1 with a P removal efficiency of 57%. As saturated ICS filters can easily be replaced and recycled for other applications, this is a promising sustainable technique to drastically cut back diffuse P losses and to tremendously improve surface water quality in the short term.


Assuntos
Ferro/química , Minerais/química , Fósforo/química , Dióxido de Silício/química , Poluentes Químicos da Água/química , Poluição da Água/prevenção & controle , Agricultura/métodos , Silicatos de Alumínio/química , Eutrofização , Compostos Ferrosos/química , Compostos de Ferro/química , Compostos de Magnésio/química , Silicatos/química
9.
Front Microbiol ; 9: 1433, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30034375

RESUMO

Mineral nitrogen (N) availability to heterotrophic micro-organisms is known to impact organic matter (OM) decomposition. Different pathways determining the N accessibility depend to a large extent on soil structure. Contact between soil mineral and OM substrate particles can facilitate N transport toward decomposition hot spots. However, the impact of soil structure on N availability to microbes and thus heterotrophic microbial activity and community structure is not yet fully understood. We hypothesized that carbon mineralization (Cmin) from low-N substrate would be stimulated by increased N availability caused by closer contact with soil particles or by a higher moisture level, enhancing potential for N-diffusion. Under opposite conditions retarded heterotrophic activity and a dominance of fungi were expected. A 128-days incubation experiment with CO2 emission monitoring from artificially reconstructed miniature soil cores with contrasting soil structures, viz. high or low degree of contact between soil particles, was conducted to study impacts on heterotrophic activity. The soil cores were subjected to different water filled pore space percentages (25 or 50% WFPS) and amended with either easily degradable OM high in N (grass) or more resistant OM low in N (sawdust). X-ray µCT image processing allowed to quantify the pore space in 350 µm around OM substrates, i.e., the microbial habitat of involved decomposers. A lower local porosity surrounding sawdust particles in soils with stonger contact was confirmed, at least at 25% WFPS. Mineral N addition to sawdust amended soils with small particle contact at 25% WFPS resulted in a stimulated respiration. Cmin in the latter soils was lower than in case of high particle contact. This was not observed for grass substrate particles or at 50% WFPS. The interactive effect of substrate type and soil structure suggests that the latter controls Cmin through mediation of N diffusion and in turn N availability. Phospholipid fatty acid did not reveal promotion of fungal over bacterial biomarkers in treatments with N-limited substrate decomposition. Combining X-ray µCT with tailoring soil structure allows for more reliable investigation of effects on the soil microbial community, because as also found here, the established soil pore network structure can strongly deviate from the intended one.

10.
Waste Manag ; 61: 494-505, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28057418

RESUMO

Separating dairy cattle slurry in a liquid and solid fraction (SF) is gaining more interest, since it enables a more targeted use of both fractions. However, the valorization of the SF is limited on P-rich soils, due to its high P content, and the export or use as bedding material requires sanitation. Therefore, we investigated the influence of composting or ensiling the SF, whether or not mixed with bulking agents, on the product quality in terms of fertilizer value, sanitation and stability. Ensiling can be considered as a controlled storage method for conserving C and nutrients. Soil amendment with co-ensiled SF resulted in a higher N mineralization and crop growth compared to amendment of co-composted SF. Co-composting SF with structure-rich feedstock materials optimized the composting process and sanitation when compared with composting pure SF and did not increase the risk for extreme-heat-resistant spores of thermophilic aerobic spore-forming bacteria (X-TAS). Further, the composts contained more P per unit of fresh weight than the silages, beneficial for the export of the composted SF. The oxygen uptake rate was found to be less powerful to determine the stability of fresh, composted and ensiled SF.


Assuntos
Fertilizantes , Esterco , Solo , Gerenciamento de Resíduos/métodos , Animais , Bélgica , Bovinos , Nitrogênio/metabolismo , Oxigênio/metabolismo , Silagem , Microbiologia do Solo , Esporos Bacterianos , Temperatura , Zeolitas
11.
Sci Total Environ ; 361(1-3): 189-95, 2006 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-16061272

RESUMO

Acidification of forest soils in Europe and North America has been an important concern over the last decades. The last area-covering survey of forest soil acidification in Flanders (North Belgium) goes back to 1985 [Ronse A, De Temmerman L, Guns M, De Borger R. Evolution of acidity, organic matter content, and CEC in uncultivated soils of North Belgium during the past 25 years. Soil Sci; 146, (1988), 453-460] and highlighted a significant acidification of the upper layer (0.3-0.4 m) of forested podzols during the period 1950-1985. The present study aimed to assess (1) to what extent further acidification of forested podzols occurred during the period 1985-2000 at different depths and (2) whether the average annual acidification rate accelerated or slowed down between 1985 and 2000 compared to the period 1950-1985. Average soil pH-KCl values of podzols in northern Belgium dropped during the period 1985-2000. This decline extends to a depth of about 50 cm but was most pronounced and significant in the A horizon. In the A(0), A(1) and A(2) horizons, average pH dropped with 0.2, 0.3 and 0.1 units, and in the B(ir) and C horizons with 0.1 units. No change in average pH value occurred in the B(h) horizon. Average annual acidification rate of the A(1) horizon was significantly higher in the period 1985-2000 than in the period 1950-1985. Changes in pH occurred in the entire soil profile during the period 1950/67-1985 likely because sulphate was the major form of acid deposition before 1985. After 1985, acid sulphur deposition decreased with more than 50% in North Belgium. In contrast, ammonium deposition almost doubled between 1950 and 1980, which may explain why soil acidification between 1985 and 2000 has been restricted to the upper soil horizons.


Assuntos
Chuva Ácida , Solo , Árvores , Bélgica , Monitoramento Ambiental , Concentração de Íons de Hidrogênio , Cloreto de Potássio
12.
Commun Agric Appl Biol Sci ; 71(1): 121-4, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-17191488

RESUMO

The influence of 10 years reduced tillage (RT) on the potential carbon mineralization of the 0-5 cm layer of silt loam soils in Belgium under a temperate climate was investigated. Therefore, four fields at three locations under 10 years of RT and fields under conventional tillage (CT) with comparable crop rotation were selected. The higher % soil organic carbon in the upper layer resulted in a higher potential carbon mineralization of the RT fields. The small increase in % soil organic carbon and potential carbon mineralization of RT fields was contributed to the high soil disturbance due to incorporation of manure in the upper layer and the production of sugar beets and potatoes. Simulating ploughing by emptying and refilling the soil cores resulted mostly in a higher potential carbon mineralization. However, the differences were not significant due to the high variability in potential carbon mineralization.


Assuntos
Agricultura/métodos , Carbono/metabolismo , Conservação dos Recursos Naturais , Monitoramento Ambiental/métodos , Solo/análise , Agricultura/instrumentação , Bélgica , Carbono/análise , Clima , Produtos Agrícolas/crescimento & desenvolvimento , Compostos Orgânicos/análise , Compostos Orgânicos/metabolismo , Solo/normas , Fatores de Tempo
13.
Sci Rep ; 6: 32862, 2016 09 08.
Artigo em Inglês | MEDLINE | ID: mdl-27605154

RESUMO

The role of soil fauna in crucial ecosystem services such as nutrient cycling remains poorly quantified, mainly because of the overly reductionistic approach adopted in most experimental studies. Given that increasing nitrogen inputs in various ecosystems influence the structure and functioning of soil microbes and the activity of fauna, we aimed to quantify the role of the entire soil nematode community in nutrient mineralization in an experimental set-up emulating nutrient-rich field conditions and accounting for crucial interactions amongst the soil microbial communities and plants. To this end, we reconstructed a complex soil foodweb in mesocosms that comprised largely undisturbed native microflora and the entire nematode community added into defaunated soil, planted with Lolium perenne as a model plant, and amended with fresh grass-clover residues. We determined N and P availability and plant uptake, plant biomass and abundance and structure of the microbial and nematode communities during a three-month incubation. The presence of nematodes significantly increased plant biomass production (+9%), net N (+25%) and net P (+23%) availability compared to their absence, demonstrating that nematodes link below- and above-ground processes, primarily through increasing nutrient availability. The experimental set-up presented allows to realistically quantify the crucial ecosystem services provided by the soil biota.


Assuntos
Carbono/metabolismo , Nematoides/metabolismo , Nitrogênio/metabolismo , Fenômenos Fisiológicos Vegetais , Microbiologia do Solo , Animais , Biomassa , Nematoides/microbiologia , Plantas/microbiologia , Solo/química
14.
Sci Total Environ ; 553: 107-119, 2016 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-26906698

RESUMO

We evaluated trends (2005-2013) and patterns of dissolved organic nitrogen (DON) and its ratio with dissolved organic carbon (DOC), DOC:DON in atmospheric deposition and soil solution of five Level II plots of the International Co-operative Programme on Assessment and Monitoring of Air Pollution Effects on Forests (ICP Forests) in Flanders, Northern Belgium. The primary aim was to confirm positive postulated trends in DON levels and DOC:DON under on-going recovery from acidification. The DON concentrations (0.95-1.41 mg L(-1)) and fluxes (5.6-8.3 kg ha(-1)y(-1)) in throughfall were about twice as high compared to precipitation in the open field (0.40-0.48 mg L(-1), 3.0-3.9 kg ha(-1)y(-1)). Annual soil profile leaching losses of DON varied between 1.2 and 3.7 kg ha(-1)y(-1). The highest soil DON concentrations and fluxes were observed beneath the O horizon (1.84-2.36 mg L(-1), 10.1-12.3 kg ha(-1)y(-1)). Soil solution concentrations and fluxes of DON showed significant increasing trends. Temporarily soil solution DOC:DON rose following an exceptionally long spring drought in 2007, suggesting an effect of drying and rewetting on DOM composition. Further research is needed to test the dependence of DON and DOC:DON on factors such as latitude, forest cover, length of the growing season, hydrology and topography. Nonetheless, even with considerable variation in soil type, level of base saturation, and soil texture in the five included ICP Forests Level II plots, all data revealed a proportionally larger positive response of DON flux than DOC to recovery from acidification.


Assuntos
Monitoramento Ambiental , Florestas , Nitrogênio/análise , Solo/química , Bélgica
15.
PLoS One ; 10(9): e0136244, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26393517

RESUMO

To understand the roles of nematodes in organic matter (OM) decomposition, experimental setups should include the entire nematode community, the native soil microflora, and their food sources. Yet, published studies are often based on either simplified experimental setups, using only a few selected species of nematode and their respective prey, despite the multitude of species present in natural soil, or on indirect estimation of the mineralization process using O2 consumption and the fresh weight of nematodes. We set up a six-month incubation experiment to quantify the contribution of the entire free living nematode community to carbon (C) mineralization under realistic conditions. The following treatments were compared with and without grass-clover amendment: defaunated soil reinoculated with the entire free living nematode communities (+Nem) and defaunated soil that was not reinoculated (-Nem). We also included untreated fresh soil as a control (CTR). Nematode abundances and diversity in +Nem was comparable to the CTR showing the success of the reinoculation. No significant differences in C mineralization were found between +Nem and -Nem treatments of the amended and unamended samples at the end of incubation. Other related parameters such as microbial biomass C and enzymatic activities did not show significant differences between +Nem and -Nem treatments in both amended and unamended samples. These findings show that the collective contribution of the entire nematode community to C mineralization is small. Previous reports in literature based on simplified experimental setups and indirect estimations are contrasting with the findings of the current study and further investigations are needed to elucidate the extent and the mechanisms of nematode involvement in C mineralization.


Assuntos
Carbono/metabolismo , Nematoides/metabolismo , Nitrogênio/metabolismo , Solo/química , Animais , Biomassa , Celulases/metabolismo , Ácidos Graxos/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Bactérias Gram-Negativas/metabolismo , Bactérias Gram-Positivas/metabolismo , Nematoides/enzimologia , Oxirredutases/metabolismo , Poaceae/crescimento & desenvolvimento , Poaceae/metabolismo , Microbiologia do Solo
16.
J Environ Qual ; 33(5): 1647-52, 2004.
Artigo em Inglês | MEDLINE | ID: mdl-15356224

RESUMO

The effect of soil fumigation on N mineralization and nitrification needs to be better quantified to optimize N fertilizer advice and predict NO(-)(3) concentrations in crops and NO(-)(3) leaching risks. Seven soils representing a range in soil texture and organic matter contents were fumigated with Cyanamid DD 95 (a mixture of 1,3-dichloropropane and 1,3-dichloropropene). After removal of the fumigant, the fumigated soils and unfumigated controls were incubated for 20 wk and N mineralization and nitrification were monitored by destructive sampling. The average short-term N mineralization rates (k(s)) were significantly larger in the fumigated than in the unfumigated soils (P = 0.025), but the differences in k(s) between fumigated and unfumigated soils could not be related to soil properties. The average long-term N mineralization rates (k(l)) were slightly larger in the fumigated soils but the difference with the unfumigated soils was not significant. Again, the differences in k(l) values could not be related to soil properties. Nitrification was inhibited completely for at least 3 wk in all soils, and an effect on nitrification could be observed up to 17 wk in one soil. An S-shaped function was fitted to the nitrification data corrected for N mineralization, and both the rate constant (gamma) and the time at which maximum nitrification was reached (t(max)) were strongly correlated to soil pH. However, since no correlations were found between the effect of fumigation on N mineralization and soil properties, taking into account the effects of fumigation in fertilizer advice and in the prediction of NO(-)(3) leaching risks will need further research.


Assuntos
Compostos Alílicos/química , Nitrogênio/metabolismo , Propano/análogos & derivados , Propano/química , Fertilizantes , Fumigação , Hidrocarbonetos Clorados , Medição de Risco , Solo , Poluentes do Solo/análise , Solubilidade
17.
Commun Agric Appl Biol Sci ; 68(1): 15-23, 2003.
Artigo em Inglês | MEDLINE | ID: mdl-14696233

RESUMO

This paper reports on experiences with the use of the PEST (Parameter ESTimation) programme to calibrate the soil physical parameters of a nitrate leaching model (the Burns alpha model) and to determine the N mineralization rate by inverse modelling. Parameter estimation was much more efficient and accurate with the PEST programme than with a built in "trial and error" calibration module. The determination of the N mineralization rate by inverse modelling with PEST using measured nitrate concentrations was not possible, and several reasons for this are given. With more suitable experimental fields (lighter soil texture and/or deeper groundwater level), more replications in the nitrate measurements and a one-step calibration of the alpha parameter and the N mineralization rate, determination of the N mineralization rate may become possible.


Assuntos
Modelos Teóricos , Nitratos/análise , Solo/análise , Calibragem , Simulação por Computador , Cinética , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Poluentes do Solo/análise , Poluentes da Água/análise
18.
Environ Pollut ; 189: 54-62, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24631973

RESUMO

In a forest on sandy, metal polluted soil, we examined effects of six tree species on litter decomposition rates and accompanied changes in metal (Cd, Zn) and nutrient (base cations, N, C) amounts. Decomposition dynamics were studied by means of a litterbag experiment lasting for 30 months. The decomposition peak occurred within the first year for all tree species, except for aspen. During litter decomposition, high metal litter types released part of their accumulated metals, whereas low metal litter types were characterized by a metal enrichment. Base cations, N and C were released from all litter types. Metal release from contaminated litter might involve risks for metal dispersion towards the soil. On the other hand, metal enrichment of uncontaminated litter may be ecologically relevant as it can be easily transported or serve as food source.


Assuntos
Metais/análise , Poluentes do Solo/análise , Solo/química , Carbono/análise , Monitoramento Ambiental , Cinética , Modelos Químicos , Nitrogênio/análise , Árvores/fisiologia
19.
Sci Total Environ ; 447: 99-107, 2013 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-23376521

RESUMO

Trees play an important role in the biogeochemical cycling of metals, although the influence of different tree species on the mobilization of metals is not yet clear. This study examined effects of six tree species on fluxes of Cd, Zn, DOC, H(+) and base cations in forest floor leachates on a metal polluted site in Belgium. Forest floor leachates were sampled with zero-tension lysimeters in a 12-year-old post-agricultural forest on a sandy soil. The tree species included were silver birch (Betula pendula), oak (Quercus robur and Q. petraea), black locust (Robinia pseudoacacia), aspen (Populus tremula), Scots pine (Pinus sylvestris) and Douglas fir (Pseudotsuga menziesii). We show that total Cd fluxes in forest floor leachate under aspen were slightly higher than those in the other species' leachates, yet the relative differences between the species were considerably smaller when looking at dissolved Cd fluxes. The latter was probably caused by extremely low H(+) amounts leaching from aspen's forest floor. No tree species effect was found for Zn leachate fluxes. We expected higher metal leachate fluxes under aspen as its leaf litter was significantly contaminated with Cd and Zn. We propose that the low amounts of Cd and Zn leaching under aspen's forest floor were possibly caused by high activity of soil biota, for example burrowing earthworms. Furthermore, our results reveal that Scots pine and oak were characterized by high H(+) and DOC fluxes as well as low base cation fluxes in their forest floor leachates, implying that those species might enhance metal mobilization in the soil profile and thus bear a potential risk for belowground metal dispersion.


Assuntos
Poluentes do Solo/metabolismo , Solo , Árvores/fisiologia , Animais , Bélgica , Betula/fisiologia , Cádmio/metabolismo , Carbono/metabolismo , Oligoquetos/metabolismo , Pinus/fisiologia , Pinus sylvestris/fisiologia , Folhas de Planta/química , Folhas de Planta/metabolismo , Populus/fisiologia , Pseudotsuga/fisiologia , Quercus/fisiologia , Robinia/fisiologia , Árvores/metabolismo , Zinco/metabolismo
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