Stearoyl coenzyme A desaturase 1 (SCD1) regulates foot-and-mouth disease virus replication by modulating host cell lipid metabolism and viral protein 2C-mediated replication complex formation.

The life cycle of foot-and-mouth disease virus (FMDV) is tightly regulated by host cell lipid metabolism. In previous studies, we reported downregulated expression of stearoyl coenzyme A desaturase-1 (SCD1), a key enzyme of fatty acid metabolism, in BHK-VEC cells (a virus-negative cell line derived from BKH-21 cells with persistent FMDV infection) on comparing transcriptomic data for BHK-VEC and BHK-21 cells (Y. Yuan et al., Front Cell Infect Microbiol 12:940906, 2022, https://doi.org/10.3389/fcimb.2022.940906; L. Han et al., Vet Microbiol 263:109247, 2021, https://doi.org/10.1016/j.vetmic.2021.109247). In the present study, we identify that SCD1 regulates FMDV replication. SCD1 overexpression or exogenous addition of oleic acid (OA), a product of the enzymatic activity of SCD1, increased FMDV replication in both BHK-21 cells and SCD1-knockdown cells. Overexpression of SCD1 or exogenous addition of OA restored FMDV infection and replication in BHK-VEC cells, and OA also promoted FMDV replication in BHK-21 cells with persistent FMDV infection. SCD1 recruited the nonstructural FMDV protein 2C to a detergent-resistant membrane located in the perinuclear region of cells to form replication complexes. Inhibiting SCD1 enzyme activity resulted in a significantly decreased number of FMDV replication complexes with abnormal morphology. Inhibition of SCD1 activity also effectively decreased the replication of other RNA viruses such as respiratory enteric orphan virus-3-176, poliovirus-1, enterovirus 71, and vesicular stomatitis virus. Our results demonstrate that SCD1, as a key host regulator of RNA virus replication, is a potential target for developing novel drugs against infections by RNA viruses. IMPORTANCE: Many positive-stranded RNA viruses, including foot-and-mouth disease virus (FMDV), alter host membranes and lipid metabolism to create a suitable microenvironment for their survival and replication within host cells. In FMDV-infected cells, the endoplasmic reticulum membrane is remodeled, forming vesicular structures that rely heavily on increased free fatty acids, thereby linking lipid metabolism to the FMDV replication complex. Nonstructural FMDV protein 2C is crucial for this complex, while host cell enzyme stearoyl coenzyme A desaturase 1 (SCD1) is vital for lipid metabolism. We found that FMDV infection alters SCD1 expression in host cells. Inhibiting SCD1 expression or its enzymatic activity markedly decreases FMDV replication, while supplementing oleic acid, a catalytic product of SCD1, regulates FMDV replication. Additionally, SCD1 forms part of the FMDV replication complex and helps recruit 2C to a detergent-resistant membrane. Our study provides insights into the pathogenesis of FMDV and a potential novel drug target against the virus.

Emotion regulation strategies and depression in mother-adolescent dyads: An actor-partner interdependence model approach.

Parent-adolescent emotion dynamics have attracted increasing attention in recent years because adolescence is a challenging period for both adolescents and parents. However, how emotions are coconstructed between parents and adolescents is less clear. This study examined whether mothers' and adolescents' emotion regulation strategy was linked with their own and each other's depression using the actor-partner interdependence model (APIM). The participants were 173 mother-adolescent pairs (Mother: Mage = 43.05 years old, SD = 3.78; Adolescent: Mage = 13.00 years old, SD = 0.90). The results showed that the more mothers used cognitive reappraisal, the lower their depression levels were; and the more mothers and adolescents used expressive suppression, the higher their levels of depression were. Additionally, maternal expressive suppression was associated with adolescent depressive symptoms. Moreover, the results revealed that for mothers with higher levels of expressive suppression, their adolescents' usage of expressive suppression was significantly positively related to adolescents' depression, while for those mothers with lower levels of expressive suppression, there was no significant correlation between adolescents' usage of expressive suppression and depression. The findings underscore the significance of recognising the interdependence and interconnected nature of emotions within parent-adolescent relationships for a comprehensive understanding of their emotional well-being.

Mothers' emotional expressivity in urban and rural societies: Salience and links with young adolescents' emotional wellbeing and expressivity.

This research aims to investigate the salience of mothers' emotional expressivity and its links with adolescents' emotional wellbeing and expressivity in an urban society endorsing more individualism and a rural society ascribing to more collectivism. By comparing Chinese urban (N = 283, M age = 14.13) and rural (N = 247, M age = 14.09) adolescents, this research found that urban mothers' expression of positive-dominant and positive-submissive emotions (PD and PS) were more common while expression of negative-dominant (ND) emotions was less common than rural mothers'. PD and PS had significant links with urban and rural adolescents' increased emotional expressivity and self-esteem, however, only significantly related to urban adolescents' decreased depression but not with rural adolescents'. ND had significant links with both urban and rural adolescents' expression of negative emotions, however, only significantly correlated with urban adolescents' less level of self-esteem and rural adolescents' more expression of positive emotions. No significant difference was found in the salience of urban and rural mothers' expression of negative-submissive (NS) emotions, which positively related to both urban and rural adolescents' depression and emotional expressivity. Moreover, we found that adolescents' emotional wellbeing (i.e., self-esteem and depression) mediated the relationship between mothers' emotional expressivity and adolescents' expressivity in both societies. Overall, the study findings document that the salience of mothers' emotional expressivity and its relations with adolescents' emotional adjustment differ between urban and rural societies.

Maternal supportive responses to adolescents' negative emotions serve as protective factors for adolescents' hostile attribution bias longitudinally.

Existing literature has documented that parenting links to children's hostile attribution biases (HAB). However, little is known about the role played by parental emotion socialization in children's HAB. To address this research gap, the present study investigated the role of parental responses to children's negative emotions (PRCNE) in predicting adolescents' HAB using a longitudinal study. Adolescents (N = 203; Mage = 13.61 years old at Time 1), who were recruited from a city in mainland China, reported on their mothers' PRCNE and their own HAB at two waves over a year. The results showed that mothers' supportive responses (composed of emotion-focused responses and problem-focused responses) significantly predicted adolescents' reduced HAB over time; however, PRCNE including expressive encouragement, minimization, and nonsupportive responses (composed of punitive responses and parental distress) had no significant relation with adolescents' HAB. These findings add to the existing literature investigating antecedents to adolescents' social information processing deficits and biases.

Development and validation of a scale assessing parental varied responses to Chinese adolescents' negative emotions.

OBJECTIVES: Parental responses to children's negative emotions (PRCNE) constitute a critical construct in the study of emotion socialization in children and adolescents. The objective of this research was to develop and validate a concise scale for assessing how adolescents perceive PRCNE in a Chinese context, resulting in the creation of the parental varied responses to Chinese adolescents' negative emotions (PVRCANE) scale. The PVRCANE aims to evaluate seven forms of PRCNE, including the six types encompassed by the commonly used Coping with Children's Negative Emotions Scale-Adolescent Version (CCNES-AV), as well as an additional, culturally specific category frequently seen in Chinese families: didactic talk and teaching. METHODS: In Study 1, a group of participants (N = 901, Mage = 13.39 years, SD = 0.92) completed the initial PVRCANE and the CCNES-Adolescent Version. In Study 2: A separate group of participants (N = 320, Mage = 13.54 years, SD = 0.57) filled in PVRCANE and scales evaluating parental socialization goals and adolescents' depressive symptoms. RESULTS: In Study 1, factor analyses showed that a four-factor structure best fit the data, leading to the final version of the PVRCANE, which includes 35 items. PVRCANE scores exhibited good internal consistency (Omega > 0.79) and convergent validity, demonstrated by their close correlation with traditional CCNES scores. Study 2 confirmed the PVRCANE's measurement invariance for both male and female parents and demonstrated criterion validity by showing significant relationships between PVRCANE scores, parental socialization goals, and adolescents' symptoms of depression. CONCLUSION: The PVRCANE is a brief, valid, and reliable scale for assessing PRCNE in Chinese adolescents. The study concludes with a discussion on future directions and how this research enhances the existing literature on emotion socialization, with a particular emphasis on the scale's potential application in other cultural settings.

Do parents show interpersonally oriented socialization practices for adolescents' negative emotions? Through the lens of Chinese families.

This research proposes a new framework called interpersonally oriented parental emotion socialization (inter-PES) practices to address parental socialization of adolescents' interpersonal emotional processing. This framework captures parents' interpersonal perspectives when their adolescent children experience negative emotions resulting from social interactions. In Study 1, parents (n = 925; 84.54% females; Mage = 39.86 years, SD = 4.37) recalled their PES practices. Content analysis of parents' narratives showed four components of inter-PES: perspective-taking, positive attributions to others, negative attributions to others, and concern for others. In Study 2, parents (n = 536; 57.98% females; Mage = 42.84 years, SD = 4.01) evaluated their own parenting behaviors on a newly developed scale to measure the four components mentioned above. Factor analysis supported the four-factor structure. Moreover, the four subscales demonstrated good reliabilities. In Study 3, adolescents (n = 864; 45.97% females; Mage = 14.50 years, SD = 0.77) reported their perceived maternal inter-PES using the same scale, and factor analysis again confirmed the four-factor structure. Study 3 also showed that the four components of inter-PES reported by adolescents were related to their perceptions of other commonly assessed maternal parenting variables and self-reported socioemotional development. Overall, this research develops a new tool for studying inter-PES and reveals new avenues for future research on how parents' interpersonal perspectives during emotional socialization may relate to adolescents' socioemotional outcomes. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

How does parents' psychological distress relate to adolescents' problematic gaming? The roles of parent-adolescent relationship and adolescents' emotion regulation.

Background and Aims: Emerging research has identified parents' psychological distress as a potential risk factor that increases adolescents' vulnerability to problematic gaming. This study attempted to address "why" from a relational perspective. We hypothesized that parents' psychological distress may link to adolescents' problematic gaming through the mediation of parent-child relationship quality, while the mediating effects of parent-child relationship quality may vary depending on adolescents' emotion regulation. Methods: We collected data from 4,835 parent-child dyads in China (parental age = 41.45 ± 4.53 years; adolescent age = 13.50 ± 1.00 years). Structural Equation Modeling (SEM) was utilized to analyze the relationships among the variables under study. Results: Parent-reported parental depression/anxiety was related to worse adolescent-reported parent-child relationship, which in turn related to more severe adolescent-reported problematic gaming. Moreover, the mediating effects of parent-child relationship quality were weaker when adolescents used more expressive suppression (but not cognitive reappraisal). Discussion and Conclusions: The findings of this study highlight the need to consider both parent-child relationships and adolescents' active role in their own emotion regulation in order to understand parental influence on adolescent problematic gaming.

Unpacking maternal minimization responses to children's negative emotions: "Person-oriented" versus "emotion-oriented".

This research aimed to distinguish person minimization from emotion minimization in Chinese families with adolescent children. In Study 1, a scale was developed to assess two types of minimization through expert evaluations (Mage = 35.05 years, 89.47% females) and factor analyses of mothers' reported minimization (n = 417, Mage = 42.73 years). Study 2 showed that person minimization related to adolescents' (n = 412, Mage = 13.91 years, 44.11% females) and mothers' socioemotional dysfunction and mothers' (n = 412, Mage = 41.17 years) nonsupportive responses; emotion minimization correlated with adolescents' and mothers' cognitive reappraisal and expressive suppression, and mothers' supportive responses. In Study 3, an experimental design was used, dividing adolescents into two groups: one group (n = 137, Mage = 12.64 years, 55.33% females) evaluating emotion minimization and the other group (n = 123, Mage = 12.74 years, 52.26% females) evaluating person minimization. The results showed that adolescents rated emotion minimization more positively than person minimization. Study 4 (n = 200, Mage = 13.85, 34.50% females) showed reciprocal relations between problematic communication patterns of adolescents' emotional distress and person minimization over time. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

The Quality of Life for Children with Autism Spectrum Disorder Scale: Factor Analysis, MIMIC Modeling, and Cut-Off Score Analysis.

Our purpose in this study was to further examine the psychometric properties of the Quality of Life for Children with Autism Spectrum Disorder (QOLASD-C) scale. We first investigated the factor structure and the internal consistency of the scale. The bifactor model showed good fit and strong reliability. Second, we used multiple-indicators multiple-causes (MIMIC) modeling to examine the associations between demographic variables and the QOLASD-C dimensions. Results showed differences on overall QOL based on age, race/ethnicity, and autism spectrum disorder severity, but no relationships with gender. All demographic variables were associated with one or all three subscales (i.e., interpersonal relationships, self-determination, emotional well-being) of the QOLASD-C. Third, an optimal cut-off score of 37 was identified. Implications for research and practice are discussed.

Playing Closer: Using Virtual Reality to Measure Approach Bias of Internet Gaming Disorder.

Playing online games is gradually becoming mainstream entertainment, but some people may develop Internet gaming disorder (IGD). Like other behavioral addictive diseases, one of the main characteristics of IGD is a craving for games, which also makes people tend to approach game-related clues. Recently, a few researchers have started to use the approach-avoidance task (AAT) paradigm to study the approach bias of IGD, and they also think it is an essential characteristic of IGD. However, the traditional AAT cannot provide realistic approach-avoidance behavior to stimuli, and virtual reality has been proven to provide a highly ecological environment to measure approach bias. Therefore, this study innovatively integrates virtual reality and the AAT paradigm to measure the approach bias of IGD. We found that compared with neutral stimuli, IGD spent less time approaching game-related stimuli, which indicates that it is difficult for IGD to avoid game-related situations in the virtual environment. This study also revealed that game-related content stimuli in virtual reality alone did not increase the IGD group's craving for games. These results proved that AAT in VR could cause the approach bias of IGD and provide high ecological validity and an effective tool for the intervention of IGD in the future.