Effect on genetic diversity of the absence of intraspecies preference in 2 sympatric Reticulitermes termite species (Isoptera: Rhinotermitidae).

The recombinant genotypes that can be produced when closely related species mate improve the genetic diversity of the population. Among closely related species, the link between interspecific reproduction behaviors and genetic diversity has barely been studied. Reticulitermes chinensis and R. flaviceps, which live close to each other, were used as research subjects in our study to find out how preferring conspecifics affects reproductive behavior between species. We discovered that neither R. chinensis nor R. flaviceps displayed preference behavior for conspecifics. Males of R. chinensis and R. flaviceps chased and groomed not only intraspecific females but also interspecific females. In a brief period of time, 2 mating behaviors, intra- and interspecific mating, were also observed. There were no significant differences in the duration of each behavior (tandem, grooming, and mating) between interspecies and intraspecies partners. Moreover, genetic analysis showed both interspecific mating and intraspecific mating can produce living offspring when the 2 types of mating occur in a colony. Our findings showed that there was no obvious intraspecific preference between the 2 species of termite Reticulitermes when it came to tandem, grooming, and mating, which not only makes it easier for interspecific hybridization to occur but also sheds light on the genetic diversity.

Two-Dimensional Coordination Polymer Showing Spin-Crossover Behavior with a 64 K Wide Hysteresis Loop.

A two-dimensional grid-like coordination polymer, [Fe(NCBH3)2(Py2ttz)2]·4CHCl3 (1·4CHCl3, Py2ttz = 2,5-di(pyridin-4-yl)thiazolo[5,4-d]thiazole), showed one-step complete spin crossover with unexpectedly large hysteresis loop of 64 K wide and temperature-induced excited spin-state trapping effect below 91 K.

LMTRDA: Using logistic model tree to predict MiRNA-disease associations by fusing multi-source information of sequences and similarities.

Emerging evidence has shown microRNAs (miRNAs) play an important role in human disease research. Identifying potential association among them is significant for the development of pathology, diagnose and therapy. However, only a tiny portion of all miRNA-disease pairs in the current datasets are experimentally validated. This prompts the development of high-precision computational methods to predict real interaction pairs. In this paper, we propose a new model of Logistic Model Tree for predicting miRNA-Disease Association (LMTRDA) by fusing multi-source information including miRNA sequences, miRNA functional similarity, disease semantic similarity, and known miRNA-disease associations. In particular, we introduce miRNA sequence information and extract its features using natural language processing technique for the first time in the miRNA-disease prediction model. In the cross-validation experiment, LMTRDA obtained 90.51% prediction accuracy with 92.55% sensitivity at the AUC of 90.54% on the HMDD V3.0 dataset. To further evaluate the performance of LMTRDA, we compared it with different classifier and feature descriptor models. In addition, we also validate the predictive ability of LMTRDA in human diseases including Breast Neoplasms, Breast Neoplasms and Lymphoma. As a result, 28, 27 and 26 out of the top 30 miRNAs associated with these diseases were verified by experiments in different kinds of case studies. These experimental results demonstrate that LMTRDA is a reliable model for predicting the association among miRNAs and diseases.

[Distribution of Syndrome Types of Chinese Medicine in Acute Infectious Diarrhea].

OBJECTIVE: To observe syndrome types of Chinese medicine (CM) and distribution features of acute infectious diarrhea patients. METHODS: A retrospective study was performed in 465 acute infectious diarrhea patients. The distribution of CM syndrome and syndrome types in different seasons and genders were analyzed. RESULTS: Lack of appetite [381 (81.94%)], fatigue [350 (75.27%)], abdominal pain [338 (72.69%)], tenesmus [325 (69.89%)], anal scorching hot [276 (59.35%)], nausea [25 (55.48%)], diarrhea [249 (53.55%)], short yellow-urine [240 (51.61%)], thirsty [210 (45.16%)], and abdominal distention [206 (44.30%)] were most often seen. The syndrome distribution were sequenced as intestinal damp heat syndrome [268 (57.63%)], dyspeptic retention in intestine and stomach syndrome [106 (22.80%)], cold-damp invading exterior syndrome [47 (10.11%)], Pi-Wei qi deficiency syndrome [23 (4.95%)], cold-damp disturbing Pi syndrome [21 (4.52%)]. The incidence ratio of intestinal damp heat syndrome was the highest in autumn (P < 0.01), while that of cold-damp invading exterior syndrome was the highest in winter (P < 0.01). Cold-damp disturbing Pi syndrome was more often seen in females than in males (P < 0.01), and its incidence ratio was the highest in autumn (P < 0.05). The incidence ratio of dyspeptic retention in intestine and stomach syndrome was the highest in winter (P < 0.01). CONCLUSIONS: Intestinal damp heat syndrome was the most often seen in acute infectious diarrhea. Incidence ratios of in- testinal damp heat syndrome and cold-damp disturbing Pi syndrome were higher in autumn, while those of cold-damp invading exterior syndrome and dyspeptic retention in intestine and stomach syndrome were higher in winter. Cold-damp disturbing Pi syndrome was more often seen in females.

[Association between joint of heat and noise and metabolic syndrome in steel workers].

OBJECTIVE: To analyze the relationship between joint of heat and noise, and metabolic syndrome in a steel rolling factory workers. METHODS: A total of 590 steel workers were selected as subjects by cluster sampling method from workers of a steel factory. They were investigated by face to face way with the unified questionnaire which contents included personal information, occupational history, personal history, habits and other factors. Furthermore, height, weight, waist circumference and blood pressure were measured. Referring to the 2005 International Diabetes Federation (IDF) issued by the metabolic syndrome (MS) worldwide uniform definition combines waist diagnosis MS. A database was built by Epidata 3.0 software, and data was analyzed by SPSS 17.0. RESULTS: 571 steel workers were from 22 to 60 years, mean age (41.2 -7.9) years old. The prevalence of metabolic syndrome in steel workers was 17.9%. The prevalence of metabolic syndrome of those who exposed to high temperature was 18.8%, higher than that of those who did not expose to high temperature (5.3%), there was a statistically significant difference (P < 0.05). The prevalence of metabolic syndrome of those who exposed to noise was 20.6%, higher than that of those who did not exposed to noise (14.0%) (P < 0.05). After adjusting for the effects of confounding factors, the prevalence of MS those who exposed to high temperatures and noise is 1.118 times as high as that of those who did not exposed to high temperatures and noise. CONCLUSIONS: The combined effects of heat and noise is related to the increasing prevalence of MS of steel workers.

Improving the elicitation of critical customer requirements through an understanding of their sensitivity.

Customer requirements (CRs) are the essential driven forces of product development. Constrained by the rigid budget and time allocated to product development, much attentions and resources should be paid on critical customer requirements (CCRs). Product design occurs with an increasingly frenetic pace of change in today's competitive market, and the changes of external environment will lead to the changes of CRs. Thus, involving the sensitivity of CRs toward influence factors to identify CCRs is of great significance to grasp the directions of product evolution and enhance market competitiveness. To fill this gap, this study proposes a CCRs identification method integrated Kano model and structural equation model (SEM). First, the Kano model is adopted to determine the category of each CR. Second, based on CRs' categorization, an SEM model is established to measure the sensitivity of CRs toward the turbulence of influence factors. Then the importance of each CR is calculated, and by integrating the sensitivity and importance, a four-quadrant diagram is constructed to identify the CCRs. Finally, the identification of CCRs for smartphone is implemented as an example to demonstrate the feasibility and additional value of the proposed method.

Ultrasonographic optic nerve sheath diameter as a noninvasive marker for intracranial hypotension.

BACKGROUND: Invasive intracranial pressure (ICP) can result in complications, pain, or even aggravate intracranial hypotension (IH) or headache in patients with IH. OBJECTIVE: To investigate whether ultrasonographic measurements of optic nerve sheath diameter (ONSD) could serve as a noninvasive IH marker. METHODS: Ultrasonographic ONSD was measured prior to lumbar puncture (LP) and ONSD measurements compared to LP's opening pressure. We analyzed correlations between ONSD and ICP and determined the optimal ONSD cut-off point for IH. According to their LP on admission, patients were divided into three groups: IH group, normal ICP group, and elevated ICP group. Correlations between ONSD and ICP were analyzed using Pearson's correlation. A receiver operating characteristic (ROC) curve was generated to determine the optimal cut-off point for IH. RESULTS: In total, 136 subjects (75 men, 55.1% men) were included, and 1088 ONSDs were measured. The ONSD of the IH group (2.96 ± 0.15 mm) was significantly lower than that of the normal (3.59 ± 0.33 mm) and elevated ICP groups (4.90 ± 0.42 mm, p < 0.001). There was a significant difference in ONSD within all groups (p < 0.001), and the differences between the two groups were also statistically significant. ONSD and ICP values were strongly correlated, with an r = 0.952 (95% confidence interval [CI]: 0.924-0.969; p < 0.001). After adjusting for age, diastolic blood pressure, systolic blood pressure, body mass index, waistline, and head circumference, ONSD was positively associated with ICP. ROC curve analysis revealed an area under the curve of 0.990 (95% CI: 0.975-1.000). The ONSD cut-off point for identifying decreased opening pressure on LP was 3.15 mm, with 98.3% sensitivity and 91.7% specificity. CONCLUSIONS: Ultrasonographic ONSD may be a noninvasive, valuable, and easy-to-perform bedside technology for evaluating IH.

Ultrasonographic optic nerve sheath diameter monitoring of elevated intracranial pressure: two case reports.

Ultrasonography assessments of optic nerve sheath diameter (ONSD) is a non-invasive method that may help identify elevated intracranial pressure (ICP). However, this technique was used to evaluate the elevated ICP caused by traumatic brain injury. The objective of this study was to examine clinical cases of the changes in ICP with venous sinus stenosis and venous sinus thrombosis found the advantage of this technique in the application. And we dynamically monitor ONSD and ICP as a lens for understanding the dynamic assessment for ICP. The first case of venous sinus stenosis with elevated ICP identified in real-time by changes in ONSD, which are correlated with ICP before and after stenting. Another case of venous sinus thrombosis with elevated ICP. And after treatment, the patient underwent an ultrasound ONSD examination and lumbar puncture (LP) at the 1st, the 2nd and 3rd month of follow-up. The previously enlarged ONSDs retracted and LP opening pressure gradually returned to normal. These cases indicate that ONSD examination may help dynamically assess ICP changes and evaluate the efficacy of ICP treatment. These results provide utile, evidence based, preliminary clinical recommendations and indicate that ONSD examination might be a useful method of evaluating ICP, especially if repeated evaluations are needed.

Effect of aerobic exercise and different levels of fine particulate matter (PM2.5) on pulmonary response in Wistar rats.

BACKGROUND: Exposure of particulate matter of <2.5 µm (PM2.5) has been associated with adverse respiratory and the risk of inflammation. While regular physical activity (PA) reduces the risk of many adverse health effects. This study aimed to examine the protection of exercise on adverse pulmonary health induced by PM2.5 exposures in rats. METHODS: 80 Wistar rats were randomly divided into 8 groups: Sedentary (S), Exercise (E), Sedentary+ Low concentration PM2.5 exposures (S + LPM), Exercise+Low concentration PM2.5 exposures (E + LPM), Sedentary+Medium concentration PM2.5 exposures (S + MPM), Exercise+ Medium concentration PM2.5 exposures (E + MPM), Sedentary+High concentration PM2.5 exposures (S + HPM), and Exercise+ High concentration PM2.5 exposures (E + HPM). The rats in all E-related groups went through 8-week aerobic interval treadmill training (5 days/week, 1 h/day). The PM-related groups of rats were exposed to different concentration PM2.5 exposure in Beijing. After one bout of PM exposure, the pulmonary function, structure of lung tissues and several pulmonary biomarkers were observed. RESULTS: 1) Compared with S group, following changes occurred in various S + PM2.5 exposure groups: lung tissues were seriously damaged, local bleeding, pus exudation, and inflammatory cell infiltration, as well as the decline of the SOD, CAT and GSH while the incline of Penh, Ti, Te, MDA, TNF-α and IL-1ß were observed. 2) Compared with the corresponding different concentration of S + PM2.5 exposure groups, Penh, Ti, Te, MDA, TNF-α and IL-1ß were decreased and CAT and GSH were increased in related E + PM groups respectively. CONCLUSION: In summary, the results suggest that acute PM2.5 with different concentrations can cause different degrees of adverse effects on lung, especially in medium and high concentrations. The aerobic interval training improved the pulmonary function and impeded the lesion progression, which is due to effective in impeding the oxidative stress and inflammation.

Prediction of Major Adverse Cardiovascular Events and Slow/No-Reflow by Virtual Histology Imaging After Percutaneous Interventions on Saphenous Vein Grafts.

Saphenous vein grafts disease (SVGD) is a common complication after coronary artery bypass graft (CABG) and usually treated by percutaneous coronary intervention (PCI). In this prospective cohort study, we performed virtual histology-intravascular ultrasound to investigate whether plaque composition and morphological characteristics were associated with post-PCI major adverse cardiac events (MACEs) and slow/no-reflow in patients with SVGD. Patients (n = 90) were studied (76.7% men, mean age 64.9 ± 8.2 years and mean duration of SVG 8.0 ± 3.6 years). There were 77.8% lesions with a plaque burden of at least 70%; 18 MACE incidences accumulated in 14 patients over 12 months post-PCI and slow/no-reflow was observed in 12 patients. On adjusted multivariate analysis, lesion length (hazard ratio [HR] = 1.05; 95% confidence interval [CI]: 1.01-1.08]); age of CABG (HR = 1.51 [95% CI: 1.11-2.05], and absolute necrotic core (NC) area (HR = 8.04 [95% CI: 1.86-34.73]) were independently associated with MACEs. Factors independently associated with slow/no-reflow post-PCI were preprocedure systolic blood pressure (odds ratio [OR] = 0.98; 95% CI: 0.96-0.99) and absolute NC area (OR = 2.47 (95% CI: 1.14-5.36). A cutoff value of absolute NC area at ≥1.1 mm2 may serve as a significant risk predictor for no-reflow after SVG-PCI. Factors associated with MACEs and the slow/no-reflow phenomenon following PCI of the SVG can be used in risk assessment of SVG.

Exercise and air pollutants exposure: A systematic review and meta-analysis.

This review aims to systematically review and synthesize scientific evidence for the influence of air pollution exposure and outdoor exercise on health. We conducted a literature search in the PubMed, Cochrane, EMBASE, and Web of Science for articles that evaluated the combination effect of air pollution exposure and exercise on health. Questionnaires regarding exposure history, or studies examining indoor air pollution were excluded. Each included study needs to have clear exercise intervention plan. The pooled estimates of the combination effect of air pollution exposure and outdoor exercise on health were calculated in the meta-analysis. The quality of each included study was assessed and the quality of evidence for each outcome assessed in the meta-analysis was also measured. Twenty-five studies were identified. Six studies addressed ozone exposure, four diesel exhaust exposure, six traffic-related air pollution, ten particulate matter (PM) exposure. Only peak expiratory flow (effect size [ES] = -0.238, 95% confidence interval [CI] = -0.389, -0.088) was found to be significantly decreased after exercise intervention in a polluted environment in the meta-analysis. Seven studies reported exposure to air pollutant during exercise was associated with an increased risk of airway inflammation and decrements in pulmonary function. Six studies discovered that exposure of traffic pollution or high PM during exercise may contribute to changes in blood pressure, systemic conduit artery function and micro-vascular function. The combination effect of air pollution and exercise was found to be associated with the increased risk of potential health problems of cardiopulmonary function, immune function, and exercise performance.

Do alkyl groups on aromatic or aliphatic structure directing agents affect water stabilities and properties of hybrid iodoargentates?

Two types of in situ formed structure directing agents (SDAs) including aromatic triphenylphosphine (PPh3)- and aliphatic piperazine (H2pp)-derivative cations were used to synthesize five new hybrid iodoargentates, namely (EtPPh3)Ag3I4 (1, Et = ethyl), (n-PrPPh3)Ag3I4 (2, n-Pr = n-propyl), (i-PrPPh3)Ag5I6 (3, i-Pr = isopropyl), (Me4pp)0.5AgI2 (4, Me = methyl), and (H3app)2(Ag2I6)·2I·2H2O (5, app = N-aminoethylpiperazine). A comparative study of the two types of SDAs on the structures, stabilities and properties of hybrid iodoargentates was performed in detail. Structurally, except for (EtPPh3)+ and (n-PrPPh3)+, which both directly form (Ag3I4)- anionic chains in 1 and 2, three SDAs generate hybrid iodoargentates different from each other with inorganic anions ranging from a 0-D (Ag2I6)4- dimer to 1-D α-type (AgI2)- and (Ag5I6)- chains. With regard to the electronic structures, aromatic PPh3-derivative cations make noticeable contributions to the bottom of the conduction bands, while aliphatic pp-derivative cations make nearly no contribution to the frontier orbitals, clearly indicating their different ways to adjust the band gaps. With regard to stability, the decomposition temperatures of 1-3 in the range of 324-349 °C are noticeably higher than the values of 217 and 225 °C for 4 and 5. Furthermore, 1-4 exhibit good water stabilities, which is ascribed to the alkylation reactions precluding the formation of strong hydrogen bonds between alkylated SDAs and extraneous H2O molecules. Contrarily, the presence of typical hydrophilic [double bond, length as m-dash]NH2+, [triple bond, length as m-dash]NH+ and -NH3+ groups on the protonated (H3app)3+ cation makes 5 sensitive to water and a hydrolysis reaction occurs to generate a cubic AgI phase. Finally, 1-3 exhibit high photocatalytic efficiencies for the degradation of rhodamine B (RhB) dye in wastewater under visible light. All conclusions obtained here will help a lot in the synthesis of stable functional metal halide-based hybrids.

Heteroaggregation of engineered nanoparticles and kaolin clays in aqueous environments.

The increasing and wide use of nanoparticles (NPs), including TiO2 and Ag NPs, have raised concerns due to their potential toxicity and environmental impacts. Kaolin is a very common mineral in aquatic systems, and there is a very high probability that nanoparticles (NPs) will interact with these clay minerals. We studied the effect of kaolin particles on the aggregation of NPs under different conditions, including the role of pH, ionic strength (IS), and humic acid (HA). We show that kaolin reduces the energy barrier and the Critical Coagulation Concentration (CCC) at pH 4. At pH 8, even though the energy barrier of the system without kaolin increases, kaolin promotes NP aggregation via heteroaggregation. When IS is equal to or greater than the CCC, on the one hand HA promotes aggregation of TiO2 NPs, but on the other hand HA decreases the rate of Ag NP aggregation because the existence of a surface coating may limit the adsorption of HA on these Ag NPs. In addition, the presence of HA increases the energy barrier and the CCC of the binary system (kaolin + NPs). Thus, the complex interactions of clay, NPs, IS, pH, and HA concentration determine the colloidal stability of the NPs. We find that kaolin is a potential coagulant for removal of NPs that behave like Ag and TiO2.

Neuroprotective effects of anti-tumor necrosis factor-alpha antibody on apoptosis following subarachnoid hemorrhage in a rat model.

Recent studies have emphasized the importance of apoptosis in subarachnoid hemorrhage (SAH) and the subsequent early brain injury. However, the apoptotic pathways induced by SAH in different brain regions are not fully understood. We investigated gene expression levels of classical apoptosis-related molecules (caspase-3, bax, and bcl-2) following SAH in the hippocampus of male Wistar rats. Temporally specific changes were found in caspase-3 and bax messenger RNA only. Interestingly, we found increased expression of bax, but not caspase-3, in the prefrontal cortex, which indicates different molecular mechanisms of apoptosis in distinct brain regions. Most important, changes in expression were reversed by functional blockade of tumor necrosis factor-alpha, which has a critical role in brain injury. In addition, we found that apoptosis induced by SAH may be associated with a relative elevation of pro-brain derived neurotrophic factor.