RESUMO
Repetitive activation of excitatory synapses in the hippocampus produces a persistent enhancement of synaptic efficiency known as long-term potentiation (LTP). In anesthetized and in freely moving rats, the induction of LTP in the perforant path led to a transient increase in the amount of messenger RNA (mRNA) coding for a presynaptic glutamate receptor (GR33) in dentate granule cells. The amount of GR33 mRNA was increased for at least 5 hours after the induction of LTP but was indistinguishable from control values 1 day after induction. The N-methyl-D-aspartate receptor antagonist 2-aminophosphonovalerate prevented the induction of both LTP and the increase in GR33 mRNA. The amount of GR33 protein was increased in the mossy fiber terminal zone of dentate granule cells 5 hours after the induction of LTP. These results suggest that the induction of LTP in synapses at one stage in a neural network may lead to modification in synaptic function at the next stage in the network.
Assuntos
Hipocampo/metabolismo , Receptores de Glutamato/genética , Receptores Pré-Sinápticos/genética , Sinapses/metabolismo , 2-Amino-5-fosfonovalerato/farmacologia , Animais , Estimulação Elétrica , Potenciais Evocados , Expressão Gênica , Hipocampo/fisiologia , Hibridização In Situ , Masculino , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos , Ratos Sprague-Dawley , Receptores de Glutamato/biossíntese , Receptores Pré-Sinápticos/biossínteseRESUMO
Following LTP induction in freely moving rats, in situ hybridization revealed discrete changes in the expression of one isoform in each of four families of serine/threonine kinases constitutively expressed in the dentate gyrus of the hippocampus. Expression of the alpha isoform of CaMKII showed a transient increase over the soma and a more persistent increase over the dendritic field of dentate granule cells. Of the PKC isoforms, only gamma PKC was up-regulated substantially 2 hr after LTP induction, declining to control levels 48 hr later. An increase in the expression of mRNA for ERK2 and raf-B was seen at 24 hr only. These results show that, during the maintenance phase of LTP in the hippocampus, there are selective increases in the expression of serine/threonine kinases and that these increases have specific and characteristic temporal and spatial profiles.
Assuntos
Potenciação de Longa Duração , Transdução de Sinais , Animais , Proteínas Quinases Dependentes de Cálcio-Calmodulina/metabolismo , Dendritos/enzimologia , Regulação da Expressão Gênica , Genes Precoces , Granulócitos/enzimologia , Hipocampo/enzimologia , Hipocampo/fisiologia , Isoenzimas/metabolismo , Proteína Quinase C/metabolismo , RNA Mensageiro/metabolismo , Ratos , Ratos Sprague-Dawley , Fatores de TempoRESUMO
At room temperature (23 degrees C-25 degrees C), the induction of long-term potentiation (LTP) in area CA1 of slices from young male Sprague-Dawley rats was depressed by preincubation with the nitric oxide synthase inhibitors NG-nitro-L-arginine (L-NA, 100 microM) and NG-nitro-L-arginine methyl ester (L-NAME, 100 microM). The D isomers were ineffective under the same conditions. Hemoglobin (20 microM) reduced but did not completely block LTP. Neither L-NA (at concentrations up to 1 mM) nor hemoglobin (20 microM) had any significant effect on LTP in slices from adult rats at room temperature, or in young rats at 29 degrees C-30 degrees C. These results suggest that nitric oxide is unlikely to play a role in the induction of LTP under physiological conditions.
Assuntos
Envelhecimento/fisiologia , Aminoácido Oxirredutases/antagonistas & inibidores , Hipocampo/efeitos dos fármacos , Hipocampo/fisiologia , Potenciação de Longa Duração/efeitos dos fármacos , Temperatura , Animais , Arginina/análogos & derivados , Arginina/farmacologia , Hemoglobinas/farmacologia , Técnicas In Vitro , Masculino , NG-Nitroarginina Metil Éster , Óxido Nítrico Sintase , Nitroarginina , Ratos , Ratos Sprague-DawleyRESUMO
We have demonstrated that immediate early genes can be differentially activated within the central nervous system. We examined the effects of tetanic stimulation in the hippocampus and of noxious sensory stimulation of the spinal cord on the expression of eight immediate early genes. Induction of long-term potentiation (LTP) in the dentate gyrus resulted in an increase in mRNA and protein for NGFI-A (also termed Zif/268, Egr-1, or Krox 24), and less consistently for jun-B mRNA. No increase was seen for c-fos, NGFI-B, c-jun, jun-D, SRF, or PC4 mRNAs. Blockade of the NMDA receptor prevented the induction of both LTP and NGFI-A mRNA in the dentate gyrus. However, commissural stimulation, which prevented the induction of LTP, resulted in bilateral activation of all the genes examined, including NGFI-A. No change was seen in animals trained in a water maze. These results suggest that no simple relationship exists between LTP, spatial learning, and immediate early gene induction. Stimulation of sensory fibers resulted in an increase in mRNA for NGFI-A, c-fos, SRF, NGFI-B, and c-jun in spinal cord neurons. Blockade of the NMDA receptor had no effect on immediate early gene induction in the spinal cord.
Assuntos
Proteínas de Ligação a DNA/fisiologia , Regulação da Expressão Gênica , Hipocampo/fisiologia , Proteínas do Tecido Nervoso/genética , Medula Espinal/fisiologia , Fatores de Transcrição/fisiologia , Animais , Sequência de Bases , Estimulação Elétrica , Aprendizagem/fisiologia , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Sondas de Oligonucleotídeos , Proteínas Proto-Oncogênicas/fisiologia , Proteínas Proto-Oncogênicas c-fos , Proteínas Proto-Oncogênicas c-jun , RNA Mensageiro/genética , Ratos , Ativação TranscricionalRESUMO
The induction of long-term potentiation (LTP) in the dentate gyrus of the hippocampus is associated with a rapid and robust transcription of the immediate early gene Zif268. We used a mutant mouse with a targeted disruption of Zif268 to ask whether this gene, which encodes a zinc finger transcription factor, is required for the maintenance of late LTP and for the expression of long-term memory. We show that whereas mutant mice exhibit early LTP in the dentate gyrus, late LTP is absent when measured 24 and 48 hours after tetanus in the freely moving animal. In both spatial and non-spatial learning tasks, short-term memory remained intact, whereas performance was impaired in tests requiring long-term memory. Thus, Zif268 is essential for the transition from short- to long-term synaptic plasticity and for the expression of long-term memories.
Assuntos
Proteínas de Ligação a DNA/deficiência , Proteínas de Ligação a DNA/genética , Giro Denteado/metabolismo , Genes Precoces/fisiologia , Proteínas Imediatamente Precoces , Potenciação de Longa Duração/genética , Memória/fisiologia , Plasticidade Neuronal/genética , Fatores de Transcrição/deficiência , Fatores de Transcrição/genética , Anestésicos/farmacologia , Animais , Aprendizagem da Esquiva/fisiologia , Giro Denteado/citologia , Aprendizagem por Discriminação/fisiologia , Proteína 1 de Resposta de Crescimento Precoce , Potenciais Pós-Sinápticos Excitadores/fisiologia , Aprendizagem em Labirinto/fisiologia , Transtornos da Memória/genética , Transtornos da Memória/metabolismo , Memória de Curto Prazo/fisiologia , Camundongos , Camundongos Knockout , Neurônios/citologia , Neurônios/metabolismo , RNA Mensageiro/metabolismoRESUMO
Acetylcholine (ACh) is an important neurotransmitter in the mammalian brain; it is implicated in arousal, learning, and other cognitive functions. Recent studies indicate that nicotinic receptors contribute to these cholinergic effects, in addition to the established role of muscarinic receptors. In the hippocampus, where cholinergic involvement in learning and memory is particularly well documented, alpha7 nicotinic acetylcholine receptor subunits (alpha7 nAChRs) are highly expressed, but their precise ultrastructural localization has not been determined. Here, we describe the results of immunogold labeling of serial ultrathin sections through stratum radiatum of area CA1 in the rat. Using both anti-alpha7 nAChR immunolabeling and alpha-bungarotoxin binding, we find that alpha7 nAChRs are present at nearly all synapses in CA1 stratum radiatum, with immunolabeling present at both presynaptic and postsynaptic elements. Morphological considerations and double immunolabeling indicate that GABAergic as well as glutamatergic synapses bear alpha7 nAChRs, at densities approaching those observed for glutamate receptors in CA1 stratum radiatum. Postsynaptically, alpha7 nAChRs often are distributed at dendritic spines in a perisynaptic annulus. In the postsynaptic cytoplasm, immunolabeling is associated with spine apparatus and other membranous structures, suggesting that alpha7 nAChRs may undergo dynamic regulation, with insertion into the synapse and subsequent internalization. The widespread and substantial expression of alpha7 nAChRs at synapses in the hippocampus is consistent with an important role in mediating and/or modulating synaptic transmission, plasticity, and neurodegeneration.
Assuntos
Hipocampo/metabolismo , Hipocampo/ultraestrutura , Subunidades Proteicas , Receptores Nicotínicos/biossíntese , Animais , Bungarotoxinas/farmacocinética , Imuno-Histoquímica , Masculino , Microscopia Imunoeletrônica , Mitocôndrias/metabolismo , Mitocôndrias/ultraestrutura , Neurônios/metabolismo , Neurônios/ultraestrutura , Ratos , Ratos Sprague-Dawley , Receptores de GABA/metabolismo , Sinapses/classificação , Sinapses/metabolismo , Sinapses/ultraestrutura , Receptor Nicotínico de Acetilcolina alfa7RESUMO
Protein phosphatase inhibitor-1 (I-1) has been proposed as a regulatory element in the signal transduction cascade that couples postsynaptic calcium influx to long-term changes in synaptic strength. We have evaluated this model using mice lacking I-1. Recordings made in slices prepared from mutant animals and also in anesthetized mutant animals indicated that long-term potentiation (LTP) is deficient at perforant path-dentate granule cell synapses. In vitro, this deficit was restricted to synapses of the lateral perforant path. LTP at Schaffer collateral-CA1 pyramidal cell synapses remained normal. Thus, protein phosphatase-1-mediated regulation of NMDA receptor-dependent synaptic plasticity involves heterogeneous molecular mechanisms, in both different dendritic subregions and different neuronal subtypes. Examination of the performance of I-1 mutants in spatial learning tests indicated that intact LTP at lateral perforant path-granule cell synapses is either redundant or is not involved in this form of learning.
Assuntos
Proteínas de Transporte , Peptídeos e Proteínas de Sinalização Intracelular , Potenciação de Longa Duração/genética , Plasticidade Neuronal/genética , Fosfoproteínas Fosfatases/metabolismo , Proteínas de Ligação a RNA/genética , Animais , Giro Denteado/citologia , Giro Denteado/fisiologia , Potenciais Pós-Sinápticos Excitadores/fisiologia , Feminino , Expressão Gênica/fisiologia , Masculino , Aprendizagem em Labirinto/fisiologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Mutantes , Via Perfurante/citologia , Fosfoproteínas/metabolismo , Proteína Fosfatase 1 , Células Piramidais/química , Células Piramidais/enzimologia , Proteínas de Ligação a RNA/análise , Proteínas de Ligação a RNA/metabolismo , Percepção Espacial/fisiologia , Sinapses/química , Sinapses/enzimologia , ÁguaRESUMO
We have examined basal phosphoinositide turnover in synaptosomes obtained from the dentate gyrus of anaesthetized rats in which long-term potentiation was induced unilaterally in perforant path-granule cell synapses. Relative to the unpotentiated side, [3H]myo-inositol labelling of inositol phosphates was significantly enhanced 45 min and 3 h after induction of long-term potentiation, but reduced after 2.5 min. Similarly, [14C]arachidonic acid labelling of 1,2-diacylglycerol was increased 45 min and 3 h after induction of long-term potentiation, but reduced after 2.5 min. In a second series of experiments, induction of long-term potentiation was blocked by stimulation of the commissural projection to granule cells. In synaptosomes prepared from this tissue, there was no difference in phosphoinositide turnover between tetanized and control sides at any of the three post-tetanic intervals. We conclude that in the dentate gyrus, long-term potentiation is associated with an increase in phosphoinositide turnover which is established between 2.5 min and 45 min post-tetanus and which persists for at least 3 h.
RESUMO
We have examined the effects of the metabotropic glutamate receptor antagonist (RS)-a-methyl-4-carboxyphenylglycine (MCPG) on performance in the water maze, and on LTP in the dentate gyrus, MCPG (5 mM) reversibly blocked the induction of LTP in the perforant path-granule cell projection when perfused into the dentate gyrus of the anaesthetized rat. When injected bilaterally into the lateral ventricles, MCPG (20 mM) disrupted the performance of rats in a spatial learning version of the water maze task. In a terminal experiment, when tetanic stimulation was given to the perforant path, LTP, was found to be significantly reduced in MCPG-injected rats compared to control rats injected with vehicle. These experiments indicate that activation of MCPG-sensitive metabotropic glutamate receptors is necessary both for the full expression of LTP and spatial learning, and supply further evidence for the hypothesis that LTP provides synaptic mechanism for certain forms of learning.
Assuntos
Hipocampo/efeitos dos fármacos , Potenciação de Longa Duração/efeitos dos fármacos , Aprendizagem em Labirinto/efeitos dos fármacos , Receptores de Glutamato Metabotrópico/fisiologia , Percepção Espacial/efeitos dos fármacos , Animais , Benzoatos/administração & dosagem , Benzoatos/farmacologia , Estimulação Elétrica , Potenciais Somatossensoriais Evocados/efeitos dos fármacos , Glicina/administração & dosagem , Glicina/análogos & derivados , Glicina/farmacologia , Hipocampo/fisiologia , Injeções , Injeções Intraventriculares , Masculino , Ratos , Ratos Sprague-Dawley , Receptores de Glutamato Metabotrópico/antagonistas & inibidores , Receptores de Glutamato Metabotrópico/efeitos dos fármacosRESUMO
D(-)Aminophosphonovalerate, a specific antagonist of the N-methyl-D-aspartate subtype of glutamate receptor, was perfused through a push-pull cannula into the dentate gyrus of rats anaesthetized with urethan in order to observe its effect on the induction and maintenance of long-term potentiation and on the increase in release of endogenous glutamate associated with long-term potentiation. The amplitude of the population spike evoked by single test shocks to the perforant path was significantly depressed by 100 microM D(-)aminophosphonovalerate, but there was a minimal effect on the slope of the population excitatory postsynaptic potential, or on the concentration of glutamate released into the perfusate. A brief high-intensity tetanus given to the perforant path while D(-)aminophosphono-valerate was being perfused failed to induce long-term potentiation or the sustained increase in glutamate release associated with long-term potentiation. Short-term post-tetanic potentiation was not affected. After wash-out of D(-)aminophosphonovalerate, a second high-frequency train produced both long-term potentiation and an increase in glutamate release which was sustained for the subsequent 1 h period of observation. D(-)Aminophosphonovalerate did not suppress long-term potentiation once it had been induced. D(-)Aminophosphonovalerate (100 microM) did not itself affect in vivo release of glutamate. However, in a separate series of in vitro experiments, D(-)aminophosphonovalerate at concentrations of 50 microM and above was found to depress the Ca2+ -dependent, K+-stimulated release of preloaded [14C]-glutamate from dentate slices.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Glutamatos/metabolismo , Hipocampo/efeitos dos fármacos , Transmissão Sináptica/efeitos dos fármacos , Valina/análogos & derivados , 2-Amino-5-fosfonovalerato , Aminoácidos/metabolismo , Animais , Ácido Aspártico/metabolismo , Depressão Química , Potenciais Evocados/efeitos dos fármacos , Ácido Glutâmico , Masculino , Ratos , Ratos Endogâmicos , Valina/farmacologiaRESUMO
The dentate gyrus of anaesthetized rats was perfused with artificial cerebrospinal fluid while field responses evoked by stimulation of the perforant path were monitored. Perfusates were collected for analysis of endogenous glutamate, aspartate and arachidonate. In animals in which long-term potentiation was induced by tetanic stimulation, there was a sustained increase in the concentration of glutamate in the perfusate, and, less reliably, in aspartate, as previously reported by Bliss et al. (J. Physiol., Lond. 377, 391-408, 1986) and Errington et al. (Neuroscience 20, 279-284, 1987). The lipoxygenase and phospholipase A2 inhibitor nordihydroguaiaretic acid, when added to the perfusate 30 min before the tetanus, abolished both long-term potentiation of the population excitatory postsynaptic potential and the tetanus-induced increase in glutamate release. Long-term potentiation of the population spike was reduced but not abolished. There was also a sustained increase in the release of arachidonic acid following the induction of long-term potentiation which did not occur when induction was blocked by nordihydroguaiaretic acid. These results are discussed in the light of the possibility that arachidonic acid or one of its lipoxygenase metabolites may be the retrograde messenger which we have postulated is released from postsynaptic sites following tetanic stimulation to trigger increased transmitter release from presynaptic terminals.
Assuntos
Adaptação Fisiológica , Ácidos Araquidônicos/metabolismo , Catecóis/farmacologia , Glutamatos/metabolismo , Hipocampo/fisiologia , Masoprocol/farmacologia , Potenciais de Ação/efeitos dos fármacos , Animais , Ácido Araquidônico , Estimulação Elétrica , Ácido Glutâmico , Hipocampo/efeitos dos fármacos , Hipocampo/metabolismo , Masculino , Ratos , Ratos EndogâmicosRESUMO
In anaesthetised rats, long-term potentiation (LTP) was induced unilaterally in the dentate gyrus by tetanic stimulation of the perforant path. Animals were killed 6 h after LTP induction and dendritic spines and synapses in tetanised and untetanised (contralateral) hippocampal tissue from the middle molecular layer (MML) were examined in the electron microscope using stereological analysis. Three-dimensional reconstructions were also used for the first time in LTP studies in vivo, with up to 130 ultrathin serial sections analysed per MML dendritic segment. A volume sampling procedure revealed no significant changes in hippocampal volume after LTP and an unbiased counting method demonstrated no significant changes in synapse density in potentiated compared with control tissue. In the potentiated hemisphere, there were changes in the proportion of different spine types and their synaptic contacts. We found an increase in the percentage of synapses on thin dendritic spines, a decrease in synapses on both stubby spines and dendritic shafts, but no change in the proportion of synapses on mushroom spines. Analysis of three-dimensional reconstructions of thin and mushroom spines following LTP induction revealed a significant increase in their volume and area. We also found an increase in volume and area of unperforated (macular) and perforated (segmented) postsynaptic densities. Our data demonstrate that whilst there is no change in synapse density 6 h after the induction of LTP in vivo, there is a considerable restructuring of pre-existing synapses, with shaft and stubby spines transforming to thin dendritic spines, and mushroom spines changing only in shape and volume.
Assuntos
Giro Denteado/fisiologia , Giro Denteado/ultraestrutura , Potenciação de Longa Duração/fisiologia , Plasticidade Neuronal , Sinapses/fisiologia , Sinapses/ultraestrutura , Animais , Espinhas Dendríticas/ultraestrutura , Processamento de Imagem Assistida por Computador , Imageamento Tridimensional , Masculino , Microscopia Eletrônica , Ratos , Ratos Sprague-DawleyRESUMO
Using apolipoprotein E knockout mice derived from the Maeda source [Piedrahita J. A. et al. (1992) Proc. natn. Acad Sci. US.A. 89, 4471 4475], we have studied the influence of apolipoprotein E gene deletion on normal CNS function by neurological tests and water maze learning, hippocampal ultrastructure assessed by quantitative immunocytochemistry and electron microscopy, CNS plasticity, i.e. hippocampal long-term potentiation and amygdaloid kindling, and CNS repair, i.e. synaptic recovery in the hippocampus following deafferentation. In each study there was little difference between the apolipoprotein E knockout mice and wild-type controls of similar age and genetic background. Apolipoprotein E knockout mice aged eight months demonstrated accurate spatial learning and normal neurological function. Synaptophysin and microtubule-associated protein 2 immunohistochemistry and electron microscopic analysis of these animals revealed that the hippocampal synaptic and dendritic densities were similar between genotypes. The induction and maintenance of kindled seizures and hippocampal long-term potentiation were indistinguishable between groups. Finally, unilateral entorhinal cortex lesions produced a marked loss of hippocampal synaptophysin immunoreactivity in both groups and a marked up-regulation of apolipoprotein E in the wild-type group. Both apolipoprotein E knockout and wild-type groups showed immunohistochemical evidence of reactive synaptogenesis, although the apolipoprotein E knockout group may have initially shown greater synaptic loss. It is suggested that either apolipoprotein E is of no importance in the maintenance of synaptic integrity and in processes of CNS plasticity and repair, or more likely, alternative (apolipo)proteins may compensate for the loss of apolipoprotein E in the knockout animals.
Assuntos
Apolipoproteínas E/genética , Comportamento Animal/fisiologia , Camundongos Knockout/fisiologia , Camundongos Knockout/psicologia , Animais , Apolipoproteínas E/metabolismo , Giro Denteado/citologia , Giro Denteado/ultraestrutura , Eletrofisiologia , Córtex Entorrinal/fisiologia , Proteína GAP-43/metabolismo , Hipocampo/citologia , Hipocampo/metabolismo , Imuno-Histoquímica , Excitação Neurológica/fisiologia , Camundongos , Camundongos Knockout/anatomia & histologia , Proteínas Associadas aos Microtúbulos/metabolismo , Neurônios/citologia , Sinapses/ultraestrutura , Sinaptofisina/metabolismoRESUMO
1. The ratio of the content of vasopressin to that of oxytocin (V/O ratio) was estimated in the supraoptic nucleus (SON), paraventricular nucleus (PVN) and posterior pituitary gland (PIT) of guinea-pigs.2. Extracts were assayed for antidiuretic activity to estimate vasopressin and for milk-ejecting activity to estimate oxytocin. In assays for milk-ejecting activity, trypsin was used to inactivate vasopressin in the extracts.3. The mean V/O ratios in the SON, PVN and PIT were 28, 8.5 and 7.0 respectively in male guinea-pigs, 6.8, 7.4 and 6.9 in non-lactating females, and 5.1, 3.3 and 6.6 in lactating females.4. The distribution of the hormones within the hypothalamus is discussed in relation to their independent release in response to electrical stimulation of the SON and PVN.
Assuntos
Sistema Hipotálamo-Hipofisário/análise , Ocitocina/análise , Vasopressinas/análise , Animais , Química Encefálica/efeitos dos fármacos , Feminino , Cobaias , Técnicas In Vitro , Lactação/efeitos dos fármacos , Masculino , Ocitocina/isolamento & purificação , Hipófise/análise , Neuro-Hipófise/análise , Gravidez , Extratos de Tecidos/análise , Extratos de Tecidos/farmacologia , Tripsina , Vasopressinas/antagonistas & inibidores , Vasopressinas/isolamento & purificaçãoRESUMO
Long-term potentiation (LTP) is a widely studied model of the kind of activity-dependent modulation of synaptic efficacy which is assumed to provide the physical basis for learning. Whether LTP, in the hippocampus or elsewhere in the brain, does in fact serve such a role is still a matter for debate. One approach to answering this question is to identify physiological or biochemical changes which are common to both learning and LTP; in the hippocampus, for example, one can ask whether the biochemical changes associated with LTP are also associated with learning. In this chapter we summarize the results which we have obtained in a study of glutamate release and phosphoinositide turnover in the dentate gyrus of rats trained in a classical conditioning task. The similarity between the changes occurring after classical conditioning and those associated with LTP is consistent with the hypothesis that LTP is one of the mechanisms by which a neural trace of the learned association is formed. We discuss this interpretation in the light of the observation that classical conditioning does not appear to affect synaptic responses in the hippocampus.
Assuntos
Condicionamento Clássico/fisiologia , Glutamatos/metabolismo , Hipocampo/fisiologia , Plasticidade Neuronal/fisiologia , Fosfatidilinositóis/metabolismo , Animais , Ácido Glutâmico , Hipocampo/metabolismo , Neurotransmissores/metabolismo , RatosRESUMO
We describe a simple method, using readily available minaturised components, for inducing and monitoring long-term potentiation (LTP) at perforant path-granule cell synapses in the dentate gyrus of the freely moving mouse. Tetanic stimulation induced LTP of the field EPSP and the population spike which persisted for more than 24 h but was not present 10 days after the tetanus. The potentiation of the population spike was proportionately greater than the potentiation of the EPSP (E-S potentiation). Induction of LTP was blocked by intraperitoneal injections of the N-methyl-D-aspartate (NMDA) receptor antagonist, 3-((RS)-2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP).
Assuntos
Giro Denteado/fisiologia , Potenciação de Longa Duração , Movimento/fisiologia , Animais , Estimulação Elétrica , Antagonistas de Aminoácidos Excitatórios/farmacologia , Injeções Intraperitoneais , Potenciação de Longa Duração/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos DBA , Piperazinas/farmacologia , Receptores de N-Metil-D-Aspartato/antagonistas & inibidoresRESUMO
The release of amino acids was examined in three hippocampal areas following classical conditioning. Paired or unpaired tone(CS)-shock(US) presentations were given to animals engaged in a previously acquired food-motivated lever-pressing task. Conditioned suppression of lever-pressing was the behavioural measure of conditioning. The dentate gyrus and areas CA1 and CA3 of the hippocampus were removed bilaterally from conditioned and pseudoconditioned animals, and slices cut and stored in liquid nitrogen for subsequent in vitro analysis of the release of radiolabelled glutamate and aspartate. K+-stimulated release of radiolabelled amino acids was analysed in the presence and absence of extracellular Ca2+. Potassium-stimulated, Ca2+-dependent release of [3H]glutamate was significantly greater in slices of dentate gyrus and area CA1 prepared from conditioned animals than from pseudoconditioned animals. This finding identifies a neurochemical change associated with classical conditioning which is similar to the increase in transmitter release seen in hippocampal long-term potentiation (LTP), and which is consistent with the hypothesis that an LTP-like mechanism is involved in mnemonic processes.
Assuntos
Condicionamento Clássico/fisiologia , Glutamatos/metabolismo , Hipocampo/metabolismo , Animais , Ácido Aspártico/metabolismo , Cálcio/farmacologia , Condicionamento Operante/fisiologia , Ácido Glutâmico , Masculino , Potássio/farmacologia , Ratos , Ratos Endogâmicos , Sinapses/fisiologia , Transmissão SinápticaRESUMO
The possible importance of opioid peptides in the induction of long-term potentiation (LTP) was investigated in the perforant path-granule cell system. A high-frequency train (400 Hz) was delivered to the lateral or medial perforant path, during push-pull perfusion of the dentate molecular layer with artificial cerebrospinal fluid (CSF) alone, or with CSF containing naloxone (10(-4) M). Naloxone effectively blocked the induction, but not the maintenance of LTP in the lateral perforant path, a putative proenkephalin system. Naloxone did not affect the production of LTP in the medial pathway. These findings suggest that activation of naloxone-sensitive receptors is necessary for the full expression of LTP in the lateral perforant pathway.
Assuntos
Encéfalo/fisiologia , Naloxona/farmacologia , Animais , Encéfalo/efeitos dos fármacos , Estimulação Elétrica , Potenciais Evocados/efeitos dos fármacos , Masculino , Perfusão , Ratos , Ratos EndogâmicosRESUMO
Changes in protein content of push-pull cannula perfusates from the dentate gyrus of anaesthetized rats were analyzed before and after the induction of long-term potentiation (LTP). LTP, induced by either high-frequency stimulation of the perforant path or raising the extracellular calcium concentration, was associated with increases in the protein content of the perfusates. Tetanically induced LTP was accompanied with a large but delayed increase (apparent in the second hour after the stimulation) in protein efflux. In contrast, when LTP was induced by the elevation of extracellular calcium concentration, a smaller but more immediate increase in protein efflux was observed. When 5-D-aminophosphonovalerate was used to block the induction of LTP, no increase was observed in either case. These results indicate that LTP in the dentate gyrus is accompanied by an increase in the efflux of proteins into push-pull cannula perfusates. The possible origins of these proteins and their role in LTP are discussed.
Assuntos
Hipocampo/fisiologia , Proteínas do Tecido Nervoso/metabolismo , 2-Amino-5-fosfonovalerato , Potenciais de Ação , Anestesia , Animais , Cálcio/farmacologia , Estimulação Elétrica , Hipocampo/efeitos dos fármacos , Hipocampo/metabolismo , Masculino , Peso Molecular , Perfusão , Ratos , Ratos Endogâmicos , Fatores de Tempo , Valina/análogos & derivados , Valina/farmacologiaRESUMO
In a previous communication, we reported an increase in protein efflux into perfusates obtained from push-pull cannulation of the dentate gyrus, following induction of long-term potentiation (LTP) in the perforant path. LTP was accompanied by a delayed but general increase in protein efflux. Protein B5 (MW 14 kDa), because of its relatively high concentration in the perfusates and absence from serum, has been chosen for further characterization. Spectrophotometric analysis, in situ proteolysis, two-dimensional electrophoresis and immunoblotting, revealed that protein B5 is indistinguishable from haemoglobin. A persistent increase in an 80 kDa protease, detected by SDS-PAGE zymography, was also seen after the induction of LTP. We consider the possibility that the increased haemoglobin content of perfusates after the induction of LTP may reflect an LTP-associated increase in the release (or activation) of proteases into extracellular space, leading to proteolytic breakdown of blood clots in the vicinity of the cannula. Evidence in favour of this hypothesis is provided.