RESUMO
Several pathways control time to flowering in Arabidopsis thaliana through transcriptional and posttranscriptional gene regulation. In recent years, mRNA processing has gained interest as a critical regulator of flowering time control in plants. However, the molecular mechanisms linking RNA splicing to flowering time are not well understood. In a screen for Arabidopsis early flowering mutants we identified an allele of BRR2a. BRR2 proteins are components of the spliceosome and highly conserved in eukaryotes. Arabidopsis BRR2a is ubiquitously expressed in all analyzed tissues and involved in the processing of flowering time gene transcripts, most notably FLC. A missense mutation of threonine 895 in BRR2a caused defects in FLC splicing and greatly reduced FLC transcript levels. Reduced FLC expression increased transcription of FT and SOC1 leading to early flowering in both short and long days. Genome-wide experiments established that only a small set of introns was not correctly spliced in the brr2a mutant. Compared to control introns, retained introns were often shorter and GC-poor, had low H3K4me1 and CG methylation levels, and were often derived from genes with a high-H3K27me3-low-H3K36me3 signature. We propose that BRR2a is specifically needed for efficient splicing of a subset of introns characterized by a combination of factors including intron size, sequence and chromatin, and that FLC is most sensitive to splicing defects.
Assuntos
Proteínas de Arabidopsis/fisiologia , Arabidopsis/fisiologia , Flores/fisiologia , Proteínas de Domínio MADS/genética , Splicing de RNA , Sequência de Aminoácidos , Animais , Arabidopsis/genética , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/genética , Genes de Plantas , Humanos , Íntrons , Dados de Sequência Molecular , Mutação , RNA Mensageiro/genética , Homologia de Sequência de AminoácidosRESUMO
Balanced maternal and paternal genome contributions are a requirement for successful seed development. Unbalanced contributions often cause seed abortion, a phenomenon that has been termed "triploid block." Misregulation of imprinted regulatory genes has been proposed to be the underlying cause for abnormalities in growth and structure of the endosperm in seeds with deviating parental contributions. We identified a mutant forming unreduced pollen that enabled us to investigate direct effects of unbalanced parental genome contributions on seed development and to reveal the underlying molecular mechanism of dosage sensitivity. We provide evidence that parent-of-origin-specific expression of the Polycomb group (PcG) gene MEDEA is causally responsible for seed developmental aberrations in Arabidopsis seeds with increased paternal genome contributions. We propose that imprinted expression of PcG genes is an evolutionary conserved mechanism to balance parental genome contributions in embryo nourishing tissues.
Assuntos
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Genes de Plantas , Impressão Genômica/genética , Ploidias , Proteínas Repressoras/genética , Alelos , Sequência de Aminoácidos , Arabidopsis/citologia , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/metabolismo , Cruzamentos Genéticos , Diploide , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Teste de Complementação Genética , Homozigoto , Proteínas de Domínio MADS/genética , Proteínas de Domínio MADS/metabolismo , Dados de Sequência Molecular , Mutação/genética , Fenótipo , Pólen/citologia , Pólen/genética , Proteínas do Grupo Polycomb , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Proteínas Repressoras/metabolismo , Sementes/genética , Sementes/crescimento & desenvolvimentoRESUMO
Plants use different classes of photoreceptors to collect information about their light environment. Cryptochromes are blue light photoreceptors that control deetiolation, entrain the circadian clock, and are involved in flowering time control. Here, we describe the cry1-L407F allele of Arabidopsis (Arabidopsis thaliana), which encodes a hypersensitive cryptochrome1 (cry1) protein. Plants carrying the cry1-L407F point mutation have elevated expression of CONSTANS and FLOWERING LOCUS T under short-day conditions, leading to very early flowering. These results demonstrate that not only the well-studied cry2, with an unequivocal role in flowering promotion, but also cry1 can function as an activator of the floral transition. The cry1-L407F mutants are also hypersensitive toward blue, red, and far-red light in hypocotyl growth inhibition. In addition, cry1-L407F seeds are hypersensitive to germination-inducing red light pulses, but the far-red reversibility of this response is not compromised. This demonstrates that the cry1-L407F photoreceptor can increase the sensitivity of phytochrome signaling cascades. Molecular dynamics simulation of wild-type and mutant cry1 proteins indicated that the L407F mutation considerably reduces the structural flexibility of two solvent-exposed regions of the protein, suggesting that the hypersensitivity might result from a reduced entropic penalty of binding events during downstream signal transduction. Other nonmutually exclusive potential reasons for the cry1-L407F gain of function are the location of phenylalanine-407 close to three conserved tryptophans, which could change cry1's photochemical properties, and stabilization of ATP binding, which could extend the lifetime of the signaling state of cry1.
Assuntos
Proteínas de Arabidopsis/genética , Arabidopsis/fisiologia , Criptocromos/genética , Flores , Mutação Puntual , Alelos , Sequência de Aminoácidos , Arabidopsis/genética , Proteínas de Arabidopsis/química , Criptocromos/química , Luz , Modelos Moleculares , Simulação de Dinâmica Molecular , Dados de Sequência Molecular , Homologia de Sequência de AminoácidosRESUMO
Chromatin states change dramatically during plant development. Globally, cytologically defined heterochromatin increases during cell differentiation and organ maturation, while it decreases during callus formation and protoplastization. Interestingly, around the time of bolting, heterochromatin content of leaf nuclei decreases transiently. Locally, chromatin compactness of the regulatory gene GLABRA2 is controlled by positional cues and correlates with transcriptional activity. In the case of the flowering time regulator FLC, chromatin compactness and histone modifications are controlled by environmental cues and ensure faithful maintenance of gene repression after vernalization. The combination of cytological studies, locus-specific analyses, and novel genome-wide profiling techniques should soon lead to a more detailed understanding of the mechanisms coupling intranuclear architecture and development.
Assuntos
Cromatina/metabolismo , Desenvolvimento Vegetal , Desdiferenciação Celular/fisiologia , Núcleo Celular/fisiologia , Temperatura Baixa , Expressão Gênica , Histonas/metabolismo , Plantas/genética , Plantas/metabolismoRESUMO
BACKGROUND: Chromatin dynamics and stability are both required to control normal development of multicellular organisms. Chromatin assembly factor CAF-1 is a histone chaperone that facilitates chromatin formation and the maintenance of specific chromatin states. In plants and animals CAF-1 is essential for normal development, but it is poorly understood which developmental pathways require CAF-1 function. RESULTS: Mutations in all three CAF-1 subunits affect Arabidopsis trichome morphology and lack of CAF-1 function results in formation of trichomes with supernumerary branches. This phenotype can be partially alleviated by external sucrose. In contrast, other aspects of the CAF-1 mutant phenotype, such as defective meristem function and organ formation, are aggravated by external sucrose. Double mutant analyses revealed epistatic interactions between CAF-1 mutants and stichel, but non-epistatic interactions between CAF-1 mutants and glabra3 and kaktus. In addition, mutations in CAF-1 could partly suppress the strong overbranching and polyploidization phenotype of kaktus mutants. CONCLUSION: CAF-1 is required for cell differentiation and regulates trichome development together with STICHEL in an endoreduplication-independent pathway. This function of CAF-1 can be partially substituted by application of exogenous sucrose. Finally, CAF-1 is also needed for the high degree of endoreduplication in kaktus mutants and thus for the realization of kaktus' extreme overbranching phenotype.
Assuntos
Arabidopsis/anatomia & histologia , Proteínas Cromossômicas não Histona/metabolismo , Proteínas de Ligação a DNA/metabolismo , Folhas de Planta/anatomia & histologia , Arabidopsis/citologia , Arabidopsis/efeitos dos fármacos , Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Diferenciação Celular/efeitos dos fármacos , Fator 1 de Modelagem da Cromatina , Proteínas Cromossômicas não Histona/genética , DNA de Plantas/metabolismo , Proteínas de Ligação a DNA/genética , Epistasia Genética , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Histonas/metabolismo , Modelos Biológicos , Mutação/genética , Fenótipo , Folhas de Planta/citologia , Folhas de Planta/efeitos dos fármacos , Ploidias , Sacarose/farmacologia , Regulação para Cima/efeitos dos fármacosRESUMO
Gene expression patterns are important determinants of a cell's state, and changes in the expression profile indicate adaptation processes as a response to developmental transitions or environmental changes. Assaying gene expression can, therefore, help to elucidate mechanisms of determination and differentiation, as well as signaling networks. Several methods have been employed to determine transcript levels. The most quantitative and widely used technique is reverse transcription coupled to quantitative real time polymerase chain reaction (RT-qPCR). Live observation of fluorescence and, therefore, product increase during RT-qPCR allows the accurate determination of differences between initial template amounts. This is in contrast to the end-point analysis of conventional PCR, where initial differences in template amounts are usually masked because the analysis is done at the plateau phase. In the plateau phase, differences can no longer be distinguished due to inherent characteristics of PCR (e.g., loss of activity of the polymerase or because reaction components become limiting) that cause a drop in amplification efficiency, so that product accumulation levels out. Real time PCR circumvents this problem by shifting the analysis to an earlier stage of the amplification reaction.
Assuntos
Plantas/genética , Reação em Cadeia da Polimerase/métodos , DNA Complementar/genética , Biologia do Desenvolvimento/métodos , Regulação da Expressão Gênica de Plantas , Desenvolvimento Vegetal , RNA de Plantas/genética , RNA de Plantas/isolamento & purificaçãoRESUMO
Polycomb group (PcG) proteins are essential to maintain gene expression patterns during development. Transcriptional repression by PcG proteins involves trimethylation of H3K27 (H3K27me3) by Polycomb Repressive Complex 2 (PRC2) in animals and plants. PRC1 binds to H3K27me3 and is required for transcriptional repression in animals, but in plants PRC1-like activities have remained elusive. One candidate protein that could be involved in PRC1-like functions in plants is LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), because LHP1 associates with genes marked by H3K27me3 in vivo and has a chromodomain that binds H3K27me3 in vitro. Here, we show that disruption of the chromodomain of Arabidopsis thaliana LHP1 abolishes H3K27me3 recognition, releases gene silencing and causes similar phenotypic alterations as transcriptional lhp1 null mutants. Therefore, binding to H3K27me3 is essential for LHP1 protein function.
Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/crescimento & desenvolvimento , Arabidopsis/metabolismo , Proteínas Cromossômicas não Histona/metabolismo , Histonas/metabolismo , Alelos , Sequência de Aminoácidos , Arabidopsis/genética , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/genética , Sequência de Bases , Proteínas Cromossômicas não Histona/química , Proteínas Cromossômicas não Histona/genética , DNA de Plantas/genética , Inativação Gênica , Genes de Plantas , Modelos Moleculares , Dados de Sequência Molecular , Mutação , Filogenia , Plantas Geneticamente Modificadas , Ligação Proteica , Estrutura Terciária de Proteína , Homologia de Sequência de AminoácidosRESUMO
BACKGROUND: Research in plant science laboratories often involves usage of many different species, cultivars, ecotypes, mutants, alleles or transgenic lines. This creates a great challenge to keep track of the identity of experimental plants and stored samples or seeds. RESULTS: Here, we describe PlantDB - a Microsoft(R) Office Access database - with a user-friendly front-end for managing information relevant for experimental plants. PlantDB can hold information about plants of different species, cultivars or genetic composition. Introduction of a concise identifier system allows easy generation of pedigree trees. In addition, all information about any experimental plant - from growth conditions and dates over extracted samples such as RNA to files containing images of the plants - can be linked unequivocally. CONCLUSION: We have been using PlantDB for several years in our laboratory and found that it greatly facilitates access to relevant information.
RESUMO
Chromatin assembly factor CAF-1 facilitates the formation of nucleosomes on newly replicated DNA in vitro. However, the role of CAF-1 in development is poorly understood because mutants are not available in most multicellular model organisms. Biochemical evidence suggests that FASCIATA1, FASCIATA2 and MSI1 form CAF-1 in Arabidopsis thaliana. Because fasciata mutants are viable, CAF-1 is not essential for cell division in plants. Arabidopsis CAF-1 mutants have defects in shoot apical meristems; in addition, CAF-1 is required to establish seedling architecture, leaf size and trichome differentiation. CAF-1 is needed to restrict branching of trichomes on rosette leaves. Increased trichome branching in CAF-1 mutants is not strictly correlated with increased nuclear DNA content. In addition, fas2 glabra3 double mutants show an additive genetic interaction, demonstrating that CAF-1 acts genetically parallel to the GLABRA3-containing, endoreduplication-coupled trichome branching pathway. However, CAF-1 is often needed to restrict endoreduplication, because seedlings of most CAF-1 mutants have increased ploidy. Notably, in the Landsberg erecta background, loss of CAF-1 does not affect ploidy, demonstrating that loss of CAF-1 can be compensated in some Arabidopsis accessions. These results reveal that the functions of FAS1, FAS2 and MSI1 are not restricted to meristems, but are also needed to control genome replication at multiple steps of development.
Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/crescimento & desenvolvimento , Diferenciação Celular/fisiologia , Proteínas Cromossômicas não Histona/metabolismo , Proteínas de Ligação a DNA/metabolismo , Animais , Arabidopsis/anatomia & histologia , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Ciclo Celular/fisiologia , Fator 1 de Modelagem da Cromatina , Proteínas Cromossômicas não Histona/genética , Replicação do DNA , Proteínas de Ligação a DNA/genética , Células Epidérmicas , Epiderme/metabolismo , Regulação da Expressão Gênica de Plantas , Humanos , Hipocótilo/crescimento & desenvolvimento , Meristema/anatomia & histologia , Meristema/crescimento & desenvolvimento , Folhas de Planta/citologia , Folhas de Planta/crescimento & desenvolvimento , Plantas Geneticamente Modificadas , Ploidias , Subunidades Proteicas/genética , Subunidades Proteicas/metabolismo , Plântula/anatomia & histologia , Plântula/crescimento & desenvolvimentoRESUMO
Duplication of chromatin following DNA replication requires spatial reorganization of chromatin domains assisted by chromatin assembly factor CAF-1. Here, we tested the genomic consequences of CAF-1 loss and the function of chromatin assembly factor CAF-1 in heterochromatin formation. Genes located in heterochromatic regions are usually silent, and we found that this transcriptional repression persists in the absence of CAF-1 in Arabidopsis. However, using microarrays we observed that genes that are active during late S-phase, when heterochromatin is duplicated, were up-regulated in CAF-1 mutants. Arabidopsis CAF-1 mutants also have reduced cytological heterochromatin content; however, DNA methylation of pericentromeric repeats was normal, demonstrating that CAF-1 is not required for maintenance of DNA methylation. Instead, hypomethylation of the genome, which has only mild effects on the development of wild-type plants, completely arrested development of CAF-1 mutants. These results suggest that CAF-1 functions in heterochromatin formation. CAF-1 and DNA methylation, which is also needed for heterochromatin formation, have partially redundant functions that are essential for cell proliferation. Interestingly, transcriptional repression and heterochromatin compaction can be genetically separated, and CAF-1 is required only for the complete compaction of heterochromatin but not to maintain transcriptional repression of heterochromatic genes.