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1.
Anal Chem ; 2024 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-38343064

RESUMO

Advanced antifouling biosensors have garnered considerable attention for their potential for precise and sensitive analysis in complex human bodily fluids. Herein, a pioneering approach was utilized to establish a robust and versatile photoelectrochemical aptasensor by conjugating a zwitterionic peptide with a DNA strand. Specifically, the branched zwitterionic peptide (BZP) was efficiently linked to complementary DNA (cDNA) through a click reaction, forming the BZP-cDNA conjugate. This intriguing conjugate exploited the BZP domain to create an antifouling biointerface, while the cDNA component facilitated subsequent hybridization with probe DNA (pDNA). To advance the development of the aptasensor, an upgraded PDA/HOF-101/ZnO ternary photoelectrode was designed as the signal converter for the modification of the BZP-cDNA conjugate, while a bipyridinium (MCEPy) molecule with strong electron-withdrawing properties was labeled at the front end of the pDNA to form the pDNA-MCEPy signal probe. Targeting the model of mucin-1, a remarkable enhancement in the photocurrent signal was achieved through exonuclease-I-aided target recycling. Such an engineered zwitterionic peptide-DNA conjugate surpasses the limitations imposed by conventional peptide-based sensing modes, exhibiting unique advantages such as versatility in design and capability for signal amplification.

2.
Anal Chem ; 96(8): 3679-3685, 2024 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-38353671

RESUMO

Recently, organic photoelectrochemical transistor (OPECT) bioanalysis has become a prominent technique for the high-performance detection of biomolecules. However, as a sensitive index of the OPECT, the dynamic regulation transconductance (gm) is still severely deficient. Herein, this work reports a new photosensitive metal-organic framework (MOF-on-MOF) heterostructure for the effective modulation of maximum gm and natural bienzyme interfacing toward choline detection. Specifically, the bidentate ligand MOF (b-MOF) was assembled onto the UiO-66 MOF (u-MOF) by a modular assembly method, which could facilitate the charge separation and generate enhanced photocurrents and offer a biophilic environment for the immobilization of choline oxidase (ChOx) and horseradish peroxidase (HRP) through hydrogen-bonded bridges. The transconductance of the OPECT could be flexibly altered by increased light intensity to maximal value at zero gate bias, and sensitive choline detection was achieved with a detection limit of 0.2 µM. This work reveals the potential of MOF-on-MOF heterostructures for futuristic optobioelectronics.


Assuntos
Técnicas Biossensoriais , Estruturas Metalorgânicas , Estruturas Metalorgânicas/química , Peroxidase do Rábano Silvestre/química , Colina , Técnicas Biossensoriais/métodos
3.
Anal Chem ; 96(19): 7747-7755, 2024 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-38691774

RESUMO

Accurate classification of tumor cells is of importance for cancer diagnosis and further therapy. In this study, we develop multimolecular marker-activated transmembrane DNA computing systems (MTD). Employing the cell membrane as a native gate, the MTD system enables direct signal output following simple spatial events of "transmembrane" and "in-cell target encounter", bypassing the need of multistep signal conversion. The MTD system comprises two intelligent nanorobots capable of independently sensing three molecular markers (MUC1, EpCAM, and miR-21), resulting in comprehensive analysis. Our AND-AND logic-gated system (MTDAND-AND) demonstrates exceptional specificity, allowing targeted release of drug-DNA specifically in MCF-7 cells. Furthermore, the transformed OR-AND logic-gated system (MTDOR-AND) exhibits broader adaptability, facilitating the release of drug-DNA in three positive cancer cell lines (MCF-7, HeLa, and HepG2). Importantly, MTDAND-AND and MTDOR-AND, while possessing distinct personalized therapeutic potential, share the ability of outputting three imaging signals without any intermediate conversion steps. This feature ensures precise classification cross diverse cells (MCF-7, HeLa, HepG2, and MCF-10A), even in mixed populations. This study provides a straightforward yet effective solution to augment the versatility and precision of DNA computing systems, advancing their potential applications in biomedical diagnostic and therapeutic research.


Assuntos
DNA , Molécula de Adesão da Célula Epitelial , MicroRNAs , Humanos , Molécula de Adesão da Célula Epitelial/metabolismo , DNA/química , MicroRNAs/análise , MicroRNAs/metabolismo , Mucina-1/metabolismo , Mucina-1/análise , Computadores Moleculares , Células MCF-7 , Biomarcadores Tumorais/metabolismo , Biomarcadores Tumorais/análise , Membrana Celular/metabolismo , Membrana Celular/química , Células Hep G2
4.
Anal Chem ; 95(37): 14119-14126, 2023 09 19.
Artigo em Inglês | MEDLINE | ID: mdl-37683257

RESUMO

The building of practical biosensors that have anti-interference abilities against biofouling of nonspecific proteins and biooxidation of reducing agents in actual biological matrixes remains a great challenge. Herein, a robust photoelectrochemical (PEC) biosensor capable of accurate detection in human serum was pioneered through the integration of a new engineered branching peptide (EBP) into a synergetic dual-photoelectrode system. The synergetic dual-photoelectrode system involved the tandem connection of a C3N4/TiO2 photoanode and a AuPt/PANI photocathode, while the EBP as a dual-functional antifouling and recognition probe featured an inverted Y-shaped configuration with one recognition backbone and two antifouling branches. Such an EBP enables a simple procedure for electrode modification and an enhanced antifouling nature compared to a regular linear peptide (LP), as theoretically supported by the results from molecular dynamics simulations. The as-developed PEC biosensor had a higher photocurrent response and a good antioxidation property inherited from the photoanode and photocathode, respectively. Targeting the model protein biomarker of cardiac troponin I (cTnI), this biosensor achieved good performances in terms of high sensitivity, specificity, and anti-interference.


Assuntos
Incrustação Biológica , Humanos , Incrustação Biológica/prevenção & controle , Peptídeos , Troponina I , Antioxidantes , Eletrodos
5.
Anal Chem ; 95(23): 8879-8888, 2023 06 13.
Artigo em Inglês | MEDLINE | ID: mdl-37252785

RESUMO

CRISPR/Cas12a has been believed to be powerful in molecular detection and diagnostics due to its amplified trans-cleavage feature. However, the activating specificity and multiple activation mechanisms of the Cas12a system are yet to be elucidated fully. Herein, a "synergistic activator effect" is discovered, which supports an activation mechanism that a synergistic incorporation of two short ssDNA activators can promote the trans-cleavage of CRISPR/Cas12a, while either of them is too short to work independently. As a proof-of-concept example, the synergistic activator-triggered CRISPR/Cas12a system has been successfully harnessed in the AND logic operation and the discrimination of single-nucleotide variants, requiring no signal conversion elements or other amplified enzymes. Moreover, a single-nucleotide specificity has been achieved for the detection of single-nucleotide variants by pre-introducing a synthetic mismatch between crRNA and the "helper" activator. The finding of "synergistic activator effect" not only provides deeper insight into CRISPR/Cas12a but also may facilitate its expanded application and power the exploration of the undiscovered properties of other CRISPR/Cas systems.


Assuntos
Técnicas Biossensoriais , Sistemas CRISPR-Cas , DNA de Cadeia Simples , Nucleotídeos , RNA Guia de Sistemas CRISPR-Cas
6.
Anal Chem ; 95(19): 7723-7734, 2023 05 16.
Artigo em Inglês | MEDLINE | ID: mdl-37133978

RESUMO

Accurate identification of cancer cells is an essential prerequisite for cancer diagnosis and subsequent effective curative interventions. The logic-gate-assisted cancer imaging system that allows a comparison of expression levels between biomarkers, rather than just reading biomarkers as inputs, returns a more comprehensive logical output, improving its accuracy for cell identification. To fulfill this key criterion, we develop a compute-and-release logic-gated double-amplified DNA cascade circuit. This novel system, CAR-CHA-HCR, consists of a compute-and-release (CAR) logic gate, a double-amplified DNA cascade circuit (termed CHA-HCR), and a MnO2 nanocarrier. CAR-CHA-HCR, a novel adaptive logic system, is designed to logically output the fluorescence signals after computing the expression levels of intracellular miR-21 and miR-892b. Only when miR-21 is present and its expression level is above the threshold CmiR-21 > CmiR-892b, the CAR-CHA-HCR circuit performs a compute-and-release operation on free miR-21, thereby outputting enhanced fluorescence signals to accurately image positive cells. It is capable of comparing the relative concentrations of two biomarkers while sensing them, thus allowing accurate identification of positive cancer cells, even in mixed cell populations. Such an intelligent system provides an avenue for highly accurate cancer imaging and is potentially envisioned to perform more complex tasks in biomedical studies.


Assuntos
MicroRNAs , Neoplasias , Compostos de Manganês , Óxidos , DNA , MicroRNAs/genética , Biomarcadores , Neoplasias/diagnóstico por imagem
7.
J Sep Sci ; 45(1): 325-337, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34117714

RESUMO

Ionic chiral selectors have been received much attention in the field of asymmetric catalysis, chiral recognition, and preparative separation. It has been shown that the addition of ionic chiral selectors can enhance the recognition efficiency dramatically due to the presence of multiple intermolecular interactions, including hydrogen bond, π-π interaction, van der Waals force, electrostatic ion-pairing interaction, and ionic-hydrogen bond. In the initial research stage of the ionic chiral selectors, most of work center on the application in chromatographic separation (capillary electrophoresis, high-performance liquid chromatography, and gas chromatography). Differently, more and more attention has been paid on the spectroscopy (nuclear magnetic resonance, fluorescence, ultraviolet and visible absorption spectrum, and circular dichroism spectrum) and electrochemistry in recent years. In this tutorial review as regards the ionic chiral selectors, we discuss in detail the structural features, properties, and their application in chromatography, spectroscopy, and electrochemistry.

8.
Anal Chem ; 93(4): 2706-2712, 2021 02 02.
Artigo em Inglês | MEDLINE | ID: mdl-33426877

RESUMO

Accurate and sensitive detection of targets in practical biological matrixes such as blood, plasma, serum, or tissue fluid is a frontier issue for most biosensors since the coexistence of both potential reducing agents and protein molecules has the possibility of causing signal interference. Herein, aiming at detection in a complex environment, an advanced and robust peptide-based photocathodic biosensor, which integrated a recognition peptide with an antifouling peptide in one probe electrode, was first proposed. Selecting human chorionic gonadotropin (hCG) as a model target, the recognition peptide with the sequence PPLRINRHILTR was first anchored on the CuBi2O4/Au (CBO/Au) photocathode and then the antifouling peptide with the sequence EKEKEKEPPPPC was further anchored to generate an antifouling biointerface. The peptide-based photocathodic biosensor demonstrated excellent anti-interference to both nonspecific proteins and reducing agents because of the capability of the antifouling peptide. It also exhibited good sensitivity owing to the utilization of the recognition peptide rather than an antibody probe. This peptide-integrated method offers a new perspective for practical applications of photocathodic biosensors.


Assuntos
Técnicas Biossensoriais/instrumentação , Peptídeos/química , Fotoquímica/instrumentação , Incrustação Biológica , Técnicas Biossensoriais/métodos , Técnicas Eletroquímicas/métodos , Eletrodos , Proteínas Imobilizadas/química , Microscopia Eletrônica de Varredura , Fotoquímica/métodos , Espectroscopia Fotoeletrônica , Sensibilidade e Especificidade , Propriedades de Superfície
9.
Anal Chem ; 93(22): 7879-7888, 2021 06 08.
Artigo em Inglês | MEDLINE | ID: mdl-34038093

RESUMO

A nanoflare, a conjugate of Au nanoparticles (NPs) and fluorescent nucleic acids, is believed to be a powerful nanoplatform for diagnosis and therapy. However, it highly suffers from the nonspecific detachment of nucleic acids from the AuNP surface because of the poor stability of Au-S linkages, thereby leading to the false-positive signal and serious side effects. To address these challenges, we report the use of covalent amide linkage and functional Au@graphene (AuG) NP to fabricate a covalent conjugate system of DNA and AuG NP, label-rcDNA-AuG. Covalent coating of abundant amino groups (-NH2) onto the graphitic shell of AuG NP efficiently facilitates the coupling with carboxyl-labeled capture DNA sequences through simple, but strong, amide bonds. Importantly, such an amide-bonded nanoflare possesses excellent stability and anti-interference capability against the biological agents (nuclease, DNA, glutathione (GSH), etc.). By accurately monitoring the intracellular miR-21 levels, this covalent nanoflare is able to identify the positive cancer cells even in a mix of cancer and normal cells. Moreover, it allows for efficient photodynamic therapy of the targeted cancer cells with minimized side effects on normal cells. This work provides a facile approach to develop a superstable nanosystem showing promising potential in clinical diagnostics and therapy.


Assuntos
Grafite , Nanopartículas Metálicas , Amidas , Glutationa , Ouro
10.
Analyst ; 146(3): 848-854, 2021 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-33319869

RESUMO

Nucleic acid lateral flow sensing has drawn great research attention since it has the advantages of being simple, rapid, and cost-effective. However, considering the trace amounts of the nucleic acid targets, its sensitivity is still limited. Although enormous efforts have been devoted to enhancing its sensitivity, developing a simple lateral flow sensing platform with high sensitivity remains challenging. We report a novel lateral flow microRNA-21 biosensing platform based on a portable surface enhanced Raman scattering (SERS) reader coupled with a catalytic hairpin assembly signal amplification strategy. Hairpin DNA probes were anchored on Au@Ag nanotags, and the presence of microRNA-21 triggered the formation of numerous double-stranded DNAs along with the exposure of the biotin groups. By this means, the target was recycled and signal amplification was achieved. The Au@Ag nanoprobes with exposed biotin can be captured on the test line via its interaction with streptavidin. By scanning the strip with a portable SERS reader, the sensitive quantification of microRNA-21 was realized with a detection limit as low as 84 fM. The proposed strategy was employed to detect the target in a serum sample, demonstrating its great potential in amplified point-of-care biosensing for clinical diagnosis.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , MicroRNAs , Catálise , Ouro , Limite de Detecção , MicroRNAs/genética , Análise Espectral Raman
11.
Analyst ; 146(5): 1612-1619, 2021 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-33605973

RESUMO

ß-Cyclodextrin (ß-CD) modified silver nanoparticles (AgNPs), denoted as ß-CD/AgNPs, were prepared by a simple one-pot method. Due to the inherent chirality of ß-CD, the developed ß-CD/AgNPs exhibited higher affinity toward l-tyrosine (l-Tyr) than d-tyrosine (d-Tyr), leading to serious aggregation of AgNPs in the presence of l-Tyr. Consequently, the l-Tyr induced aggregation of AgNPs can result in signal amplification in the differential pulse voltammograms (DPVs) of l-Tyr, which can be applied for the electrochemical chiral discrimination of the Tyr enantiomers. Other chiral amino acids including tryptophan and phenylalanine can also be successfully discriminated with the ß-CD/AgNPs, suggesting high universality of the developed chiral sensor.


Assuntos
Nanopartículas Metálicas , Prata , Aminoácidos , Estereoisomerismo , Triptofano
12.
Anal Chem ; 92(16): 11469-11475, 2020 08 18.
Artigo em Inglês | MEDLINE | ID: mdl-32662629

RESUMO

For SERS analysis in living cells, the inevitable desorption of Raman molecule on the substrate surface is a key challenge. To ensure high stability, SERS systems with Raman molecules protected inside the core-Raman molecule-shell (C-M-S) structures have been designed, but at the expense of sacrificed sensing performances. Here a shell-switchable SERS blocking strategy is developed for the reliable SERS analysis in living cells, relying on the shell blockers to regulate the SERS sensing signal without affecting the internal Raman molecules. After several C-M-S structures were investigated, the SERS blocking mechanism confirmed that thick shells (Au, Ag, ZnO, and MnO2) can cause a significant reduction in the internal SERS signal by obstructing the penetration of the laser or signal. The CAu-Mpy-SAu-SMnO2 nanoprobe is designed for the ratiometric SERS sensing in living cells, which retains sensing performances even though the Raman molecule is protected inside the nanostructure. This SERS strategy makes the turn-on sensing achievable in living cells with the MnO2 shell as a signal switch and a Raman reference. Additionally, it allows for accurate monitoring of the degradation of MnO2 carriers in living cells, even without fluorescent labels.


Assuntos
Portadores de Fármacos/química , Glutationa/análise , Nanopartículas Metálicas/química , Piridinas/química , Ouro/química , Células HeLa , Humanos , Limite de Detecção , Compostos de Manganês/química , Óxidos/química , Prata/química , Análise Espectral Raman/métodos , Óxido de Zinco/química
13.
Anal Chem ; 91(19): 12298-12306, 2019 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-31486639

RESUMO

A "signal-off" surface-enhanced Raman scattering (SERS) platform has been constructed for ultrasensitive detection of miRNA-21 by integrating exonuclease-assisted target recycling amplification with a plasmon coupling enhancement effect. On this platform, Raman-labeled Au nanostar (AuNS) probes can be covalently linked with the thiolated aptamer (Apt) on the Au-decorated silicon nanowire arrays (SiNWAs/Au) substrate, creating a coupled electromagnetic field between the substrate and the probes to enhance Raman signal. In the presence of miRNA-21, T7 exonuclease specifically hydrolyzed Apt on Apt/miRNA duplex to release miRNA-21. The regenerated element could then initiate another cycle of Apt/miRNA duplex formation and Apt cleavage. Correspondingly, the capture ability of substrate toward probes and the plasmon coupling effect between them were both diminished, giving a prominent attenuation of Raman intensity that can work as the detection signal. Due to the cascading integration between the target cycle process and the plasmon coupling effect, the present platform displayed a very low detection limit (0.34 fM, 3σ) for miRNA-21 detection. Furthermore, it was proven to be effective for analyzing miRNA-21 in biological samples and distinguishing the expression levels of miRNA-21 in MCF-7 cells and NIH3T3 cells, which became a promising tool to monitor miRNA-21 in cancer auxiliary diagnosis and drug screening.


Assuntos
Técnicas Biossensoriais/instrumentação , MicroRNAs/análise , Técnicas de Amplificação de Ácido Nucleico/métodos , Análise Espectral Raman/métodos , Animais , Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais/métodos , Campos Eletromagnéticos , Exodesoxirribonucleases/genética , Ouro/química , Humanos , Limite de Detecção , Células MCF-7 , Nanopartículas Metálicas/química , Camundongos , MicroRNAs/sangue , Células NIH 3T3 , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Análise Espectral Raman/instrumentação
14.
Anal Chem ; 91(10): 6403-6407, 2019 05 21.
Artigo em Inglês | MEDLINE | ID: mdl-31062591

RESUMO

Energy transfer (ET) in photoelectrochemical (PEC) bioanalysis is usually generated between noble metal nanoparticles (NPs) and traditional inorganic quantum dots (QDs). Using the innovative polymer dot (Pdot)-involved ET, this work reports the first signal-on and cathodic PEC bioanalysis toward telomerase (TE) activity in cell extracts. Specifically, the sequential binding of capture DNA (cDNA), telomerase primer sequence (TS), and Au NP-labeled probe DNA (Au NP-pDNA) on the electrode would place the Au NPs in close proximity of the Pdots, leading to obvious quenching of the cathodic photocurrent. The subsequent extension of the TS by TE in the presence of deoxyribonucleoside triphosphates (dNTPs) would then release the Ag NP-pDNA from the electrode, leading to the recovery of the photocurrent. On the basis of the Au NP-induced photocurrent quenching and the recovery of Pdots, a sensitive biosensor could thus be developed by tracking the photocurrents to probe the TE activity. This strategy allows for signal-on and cathodic PEC bioanalysis of TE, which can be easily extended for numerous other targets of interest. We believe this work could offer a new perspective for the rational implementation of Pdot-involved ET for advanced PEC bioanalysis.


Assuntos
Transferência de Energia , Ouro/química , Nanopartículas Metálicas/química , Pontos Quânticos , Telomerase/metabolismo , Técnicas Biossensoriais , Extratos Celulares , Técnicas Eletroquímicas , Células HeLa , Humanos , Processos Fotoquímicos , Telomerase/química
15.
Anal Chem ; 91(20): 12606-12610, 2019 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-31556999

RESUMO

Currently, one of important research directions of photoelectrochemical (PEC) bioanalysis is to exploit innovative photoactive species and their elegant implementations for selective detection and signal transduction. Different from existing candidates for photoelectrode development, this study, exemplified by the cationic dipeptide nanoparticles (CDNPs), reports the first demonstration of self-assembled peptide nanostructures (SAPNs) for the PEC bioanalysis. Specifically, the CDNPs were prepared as representative materials and then immobilized onto the indium tin oxide (ITO) electrode for the PEC differentiation of several commonly involved biomolecules such as ascorbic acid (AA) and l-cysteine. Significantly, the experimental results disclosed that the CDNPs possessed unique photocathodic responses and good analytical performance toward AA detection in terms of rapid response, high stability, and excellent selectivity. This work demonstrates the great potential of the large SAPN family for the future PEC bioanalysis development and has not been reported to our knowledge.


Assuntos
Técnicas Eletroquímicas/métodos , Luz , Nanoestruturas/química , Peptídeos/análise , Ácido Ascórbico/química , Cátions/química , Cisteína/química , Eletrodos , Teoria Quântica , Compostos de Estanho/química
16.
Anal Chem ; 91(10): 6419-6423, 2019 05 21.
Artigo em Inglês | MEDLINE | ID: mdl-31033275

RESUMO

This Letter reports a novel synthetic methodology for the fabrication of three-dimensional (3D) nanostructured CdS@carbon fiber (CF) networks and the validation of its feasibility for applications as a general platform for photoelectrochemical (PEC) bioanalysis. Specifically, 3D architectures are currently attracting increasing attention in various fields due to their intriguing properties, while CdS has been most widely utilized for PEC bioanalysis applications because of its narrow band gap, proper conduction band, and stable photocurrent generation. Using CdS as a representative material, this work realized the innovative synthesis of 3D CdS@CF networks via a simple solvothermal process. Exemplified by the sandwich immunoassay of fatty-acid-binding protein (FABP), the as-fabricated 3D CdS@CF networks exhibited superior properties, and the assay demonstrated good performance in terms of sensitivity and selectivity. This work features a novel fabrication of 3D CdS@CF networks that can serve as a general platform for PEC bioanalysis. The methodology reported here is expected to inspire new interest for the fabrication of other 3D nanostructured Cd-chalcogenide (S, Se, Te)@CF networks for wide applications in biomolecular detection and beyond.


Assuntos
Compostos de Cádmio/síntese química , Fibra de Carbono/química , Técnicas Eletroquímicas/instrumentação , Processos Fotoquímicos , Sulfatos/síntese química , Compostos de Cádmio/química , Fibra de Carbono/ultraestrutura , Nanoestruturas , Sulfatos/química
17.
Anal Chem ; 91(6): 3795-3799, 2019 03 19.
Artigo em Inglês | MEDLINE | ID: mdl-30789708

RESUMO

Liposomal photoelectrochemical (PEC) bioanalysis has recently emerged and exhibited great potential in sensitive biomolecular detection. Exploration of the facile and efficient route for advanced liposomal PEC bioanalysis is highly appealing. In this work, we report the split-type liposomal PEC immunoassay system consisting of sandwich immunorecognition, CdS quantum dots (QDs)-loaded liposomes (QDLL), and the release and subsequent capture of the QDs by a separated TiO2 nanotubes (NTs) electrode. The system elegantly operated upon the protein binding and lysis treatment of CdS QDLL labels within the 96-well plate, and then the CdS QDs-enabled sensitization of TiO2 NTs electrode. Exemplified by cardiac markers troponin I (cTnI) as target, the proposed system achieved efficient activation of TiO2 NTs electrode and thus the signal generation toward the split-type PEC immunoassay. This work features the first use of QDs for liposomal PEC bioanalysis and is expected to inspire more interests in the design and implementation of numerous QDs-involved liposomal PEC bioanalysis.


Assuntos
Imunoensaio/instrumentação , Limite de Detecção , Lipossomos/química , Nanoporos , Processos Fotoquímicos , Pontos Quânticos/química , Semicondutores , Calibragem , Eletroquímica , Eletrodos
18.
Anal Chem ; 91(6): 3800-3804, 2019 03 19.
Artigo em Inglês | MEDLINE | ID: mdl-30821438

RESUMO

This work reports the liposome-mediated in situ formation of the AgI/Ag/BiOI Z-scheme heterojunction on foamed nickel electrode for signal-on cathodic photoelectrochemical (PEC) bioanalysis. Specifically, in a proof-of-concept study, Ag nanoparticle-encapsulated liposomes were initially confined via the sandwich immunobinding and then processed to release numerous Ag+ ions, which were then directed to react with the BiOI/Ni electrode, resulting in the in situ generation of a AgI/Ag/BiOI Z-scheme heterojunction on the electrode. The enhanced cathodic signal could be correlated to the target concentration, which thus underlays a novel signal-on cathodic liposomal PEC bioanalysis strategy. Different from previous anodic liposomal PEC bioanalysis, this work features the first cathodic liposomal PEC bioanalysis on the basis of the in situ formation of a Z-scheme heterojunction. More generally, integrated with various biorecognition events, this protocol could serve as a common basis for addressing numerous targets of interest.


Assuntos
Bismuto/química , Eletroquímica/instrumentação , Iodetos/química , Lipossomos/química , Níquel/química , Processos Fotoquímicos , Compostos de Prata/química , Prata/química , Eletrodos
19.
Analyst ; 144(21): 6415-6421, 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31580336

RESUMO

In this study, an electroactive rotaxane, (S,S)-crown-3, consisting of a polymeric chiral ionic liquid as a flexible axle and 18-crown-6 as the wheel, was designed and synthesized. It is worth noting that a stimuli-responsive system was developed, in which the wheel could switch its location between the chiral carbamido group and ionic pair of the ionic polymers under an external force. Next, (S,S)-crown-3 was employed as a modification on the surface of glassy electrode. In contrast to previous study, the developed probe presented a clear discrimination of an electrochemical signal in the absence of Cu(ii). Under the external force (different pH values), l-isomers of amino acids (tryptophan, tyrosine, and cysteine) could form stable host-guest interactions with the chiral carbamido group, producing higher peak currents than the d-isomers. Compared to the absence of the crown, (S,S)-crown-3 showed much better recognition efficiency. The value of IL/ID for tryptophan could reach 39.8. In brief, the present study describes a powerful method for the synthesis of an electroactive rotaxane with great enantiorecognition capability.


Assuntos
Aminoácidos/química , Rotaxanos/química , Rotaxanos/síntese química , Técnicas de Química Sintética , Eletroquímica , Análise Espectral , Estereoisomerismo
20.
Mikrochim Acta ; 186(5): 284, 2019 04 13.
Artigo em Inglês | MEDLINE | ID: mdl-30982138

RESUMO

Photocathodic methods in photoelectrochemical (PEC) analysis are based on the use of functional photocathodes. Heterojunction cathodes consisting of different kinds of semiconductors are being considered as favorite schemes when compared to the single-component ones. A semiconductor heterojunction between CuBi2O4 (CBO) and other semiconductors has not been exploited in PEC assays so far. Herein, CBO nanospheres were initially electrochemically deposited on a fluorine-doped tin oxide (FTO) conductive glass and then coupled to chemically deposited AgI nanoparticles to obtain an electrode of type AgI/CBO/FTO. It was applied as a cathode in the PEC detection of L-cysteine as a model analyte. The sensor can selectively detect L-cysteine, and it is assumed that this is due to the selective interaction between the L-cysteine and both copper and silver via the formation of Cu-S and Ag-S bonds. The photocurrent of the electrode increases linearly with the logarithm of the cysteine concentration in the range from 0.1 and 50 µM, and the detection limit is 0.1 µM. Graphical abstract Schematic presentation of the preparation of an AgI/CuBi2O4 (AgI/CBO) heterojunction on a fluorine-doped tin oxide (FTO) electrode and its application to the cathodic photoelectrochemical detection of L-cysteine.


Assuntos
Bismuto/química , Cisteína/análise , Eletroquímica/instrumentação , Flúor/química , Iodetos/química , Processos Fotoquímicos , Compostos de Prata/química , Compostos de Estanho/química , Cisteína/química , Eletrodos , Limite de Detecção , Semicondutores
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