Auxin Acts Downstream of Ethylene and Nitric Oxide to Regulate Magnesium Deficiency-Induced Root Hair Development in Arabidopsis thaliana.

This study examines the association of auxin with ethylene and nitric oxide (NO) in regulating the magnesium (Mg) deficiency-induced root hair development in Arabidopsis thaliana. With Mg deficiency, both ethylene and NO promoted the elevation of root auxin levels in roots by inducing the expression of AUXIN-RESISTANT1 (AUX1), PIN-FORMED 1 (PIN1) and PIN2 transporters. In turn, auxin stimulated ethylene and NO production by activating the activities of 1-aminocyclopropane-1-carboxylate (ACC) oxidase (ACO), ACC synthase (ACS), nitrate reductase (NR) and NO synthase-like (NOS-L). These processes constituted an NO/ethylene-auxin feedback loop. Interestingly, however, the roles of ethylene and NO in regulating Mg deficiency-induced root hair development required the action of auxin, but not vice versa. In summary, these results suggest that Mg deficiency induces a positive interaction between the accumulation of auxin and ethylene/NO in roots, with auxin acting downstream of ethylene and NO signals to regulate Mg deficiency-induced root hair morphogenesis.

Effects of soil moisture and an AC-electric field on the phytoremediation of the Cd-contaminated soil.

Phytoremediation enhanced by electric field has been considered a green and low-cost technology for remediating heavy metal-contaminated soils. Soil moisture is a main environmental factor that affects Cd availability in the soil. However, the effects of soil moisture and AC-electric field on the remediation efficiency of willow (Salix spp.) and S. Alfredii interplanted together remain unclear. In the present study, we designed four treatments (60% soil field capacity, 60% soil field capacity + 0.5 V·cm-1 AC, 100% soil field capacity, 100% soil field capacity + 0.5 V·cm-1 AC) to explore the impacts of soil moisture and AC-electric field on soil Cd availability and Cd accumulation in plants. The results showed that the application of an AC-electric field significantly increased soil Cd availability by 20.9% and 10.8% under both 60% and 100% soil field capacity, respectively. Both high water with and without AC-electric field treatments reduced the proportion of acid-extractable and reducible Cd of soil but increased the proportion of residual Cd. Compared with the control, an AC-electric field with 60% soil field capacity significantly enhanced the biomass of S. Alfredii shoots by 31.2% and increased Cd accumulation in willow leaves and S. Alfredii shoots by 14.6% and 32.3%, respectively. In addition, the biomass production of willow was significantly enhanced but the uptake of Cd by willow was dramatically decreased under an AC-electric field with high water treatment. Therefore, these results suggest that the AC-electric field combined with 60% soil field capacity may be a more promising remediation technique to clean up the Cd-contaminated soil.

Effect of AC electric field on enhancing phytoremediation of Cd-contaminated soils in different pH soils.

To increase the efficiency of phytoremediation to clean up heavy metals in soil, assisted with alternating current (AC) electric field technology is a promising choice. Our experiments utilized the hyperaccumulator Sedum alfredii Hance and the fast-growing, high-biomass willow (Salix sp.). We investigated the efficiency of AC field combined with S. alfredii-willow intercropping for removing Cd from soils with different pH values. In the AC electric field treatment with S. alfredii-willow intercropping, the available Cd content in acidic soil increased by 50.00% compared to the control, and in alkaline soil, the increase was 100.00%. Furthermore, AC electric field promoted Cd uptake by plants in both acidic and alkaline soils, with Cd accumulation in the aboveground increased by 20.52% (P < 0.05) and 11.73%, respectively. In conclusion, the integration of AC electric fields with phytoremediation demonstrates significant favorable effectiveness.

Effects of willow and Sedum alfredii Hance planting patterns on phytoremediation efficiency under AC electric field.

Heavy metal contamination to soil is tricky due to its difficult removal, long retention time, and biomagnified toxicity. The green and low-cost phytoremediation with electric field treatment and planting pattern selection is an emerging and more effective approach to remove heavy metals from soils. In this study, alternating current (AC) electric field-assisted phytoremediation was examined with different planting patterns, i.e., monoculture willow (Salix sp.), monoculture Sedum alfredii Hance, and interplanting of willow and S. alfredii. AC electric field greatly increased phytoremediation efficiency to soil cadmium (Cd) regardless of planting patterns, either single plant species of willow or S. alfredii. The Cd removal capacity of willow and S. alfredii raises apparently under 0.5 V cm-1 AC electric field. Under different planting patterns of AC electric field treatment, Cd accumulation in the whole plant by interplanting was 5.63 times higher than monoculture willow, but only 0.75 times as high as monoculture S. alfredii. The results showed that AC electric field-assisted interplanting of willow and S. alfredii is a promising remediation technique for efficiently clean-up Cd-contaminated soil.

Effects of application of rice husk biochar and limestone on cadmium accumulation in wheat under glasshouse and field conditions.

Cadmium (Cd) has seriously threatened the safe production of food crops. Passivator amendments are commonly used to control the soil Cd availability. Yet, few studies are tested to explore the effect of the combination of various amendments. Here, we investigated the effects of different amendments (2% rice husk biochar, 2% limestone, and 1% rice husk biochar + 1% limestone) on the growth and Cd accumulation of wheat in pot and field experiments. The results showed that under the low soil Cd condition, the maximum increase of soil pH (1.83) was found in the limestone treatment compared to CK in pot experiment. Compared with the CK, the treatment of rice husk biochar decreased soil Cd availability and grain Cd content by about 25% and 31.2%, respectively. In contrast, under high soil Cd condition, the highest soil pH was observed in limestone, while the lowest soil Cd availability and grain Cd concentrations were found in rice husk biochar treatment. In the field experiment, the treatment of 1% rice husk biochar + 1% limestone caused a significant increase of soil pH by about 28.2%, whereas the treatment of 2% rice husk biochar reduced soil Cd availability and grain Cd content by about 38.9% and 38.5% compared to the CK. Therefore, rice husk biochar showed great potential to reduce Cd availability and ensure safe food production.

Effects of split applications of nitrogen fertilizers on the Cd level and nutritional quality of Chinese cabbage.

Cadmium (Cd) contamination in soil is an increasingly serious problem. Management of plant nutrients has been proposed as a potentially promising strategy for minimizing Cd accumulation in crops grown in contaminated soil. This study investigated the effects of split applications of nitrogen (N) fertilizers on the Cd concentration in Chinese cabbage (Brassica chinensis L.) plants grown in Cd-contaminated soil. Compared with single applications, split applications of ammonium or urea resulted in significantly lower Cd concentrations, and higher biomass production and antioxidant-associated nutritional quality in the edible plant parts. However, when nitrate was used as the N fertilizer, there were no significant differences between the split and single applications for the same parameters. We conclude that a split application could be more beneficial than a single application method when ammonium or urea is used as the N fertilizer for vegetable cultivation in Cd-contaminated soil.

Toxic effects of graphene on the growth and nutritional levels of wheat (Triticum aestivum L.): short- and long-term exposure studies.

Increased use of graphene materials might lead to their release into the environment. However, only a few studies have investigated the impact of graphene-based materials on green plants. In the present study, effects of graphene on plant roots and shoots after 48h or 30days of hydroponic culture were evaluated to determine its phytotoxicity. Results showed that although exposure to graphene (250, 500, 1000 and 1500mgL(-1)) significantly improved root elongation, root hair production was impaired. These observations might be associated with graphene induced-oxidative stress (indicated by nitroblue tetrazolium (NBT) and Evans blue staining, malondialdehyde (MDA) estimation, and antioxidant enzyme activity assay). After 30days of graphene exposure, shoot biomass, chlorophyll content, PSII activity and levels of several nutrient elements (N, K, Ca, Mg, Fe, Zn and Cu) were reduced, indicating that graphene inhibited plant growth and photosynthesis, and caused an imbalance of nutrient homeostasis. Based on these findings, we conclude that graphene has growth-limiting effects on plants, including root hair reduction, oxidative burst, photosynthesis inhibition, and nutritional disorder.