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1.
Cell ; 163(6): 1527-38, 2015 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-26638077

RESUMO

The killifish Nothobranchius furzeri is the shortest-lived vertebrate that can be bred in the laboratory. Its rapid growth, early sexual maturation, fast aging, and arrested embryonic development (diapause) make it an attractive model organism in biomedical research. Here, we report a draft sequence of its genome that allowed us to uncover an intra-species Y chromosome polymorphism representing-in real time-different stages of sex chromosome formation that display features of early mammalian XY evolution "in action." Our data suggest that gdf6Y, encoding a TGF-ß family growth factor, is the master sex-determining gene in N. furzeri. Moreover, we observed genomic clustering of aging-related genes, identified genes under positive selection, and revealed significant similarities of gene expression profiles between diapause and aging, particularly for genes controlling cell cycle and translation. The annotated genome sequence is provided as an online resource (http://www.nothobranchius.info/NFINgb).


Assuntos
Evolução Biológica , Peixes Listrados/genética , Cromossomos Sexuais , Envelhecimento , Animais , Feminino , Genoma , Peixes Listrados/fisiologia , Masculino , Dados de Sequência Molecular , Processos de Determinação Sexual
2.
Proc Natl Acad Sci U S A ; 121(25): e2314262121, 2024 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-38861609

RESUMO

The emergence of SARS-CoV-2 variants with increased fitness has had a strong impact on the epidemiology of COVID-19, with the higher effective reproduction number of the viral variants leading to new epidemic waves. Tracking such variants and their genetic signatures, using data collected through genomic surveillance, is therefore crucial for forecasting likely surges in incidence. Current methods of estimating fitness advantages of variants rely on tracking the changing proportion of a particular lineage over time, but describing successful lineages in a rapidly evolving viral population is a difficult task. We propose a method of estimating fitness gains directly from nucleotide information generated by genomic surveillance, without a priori assigning isolates to lineages from phylogenies, based solely on the abundance of single nucleotide polymorphisms (SNPs). The method is based on mapping changes in the genetic population structure over time. Changes in the abundance of SNPs associated with periods of increasing fitness allow for the unbiased discovery of new variants, thereby obviating a deliberate lineage assignment and phylogenetic inference. We conclude that the method provides a fast and reliable way to estimate fitness advantages of variants without the need for a priori assigning isolates to lineages.


Assuntos
COVID-19 , Genoma Viral , Filogenia , Polimorfismo de Nucleotídeo Único , SARS-CoV-2 , COVID-19/virologia , COVID-19/epidemiologia , COVID-19/genética , SARS-CoV-2/genética , SARS-CoV-2/classificação , SARS-CoV-2/isolamento & purificação , Humanos , Aptidão Genética , Genômica/métodos
3.
Eur J Immunol ; 53(12): e2350577, 2023 12.
Artigo em Inglês | MEDLINE | ID: mdl-37593947

RESUMO

Comparative phylogenetic analyses are of potential value to establish the essential components of genetic networks underlying physiological traits. For species that naturally lack particular lymphocyte lineages, we show here that this strategy readily distinguishes trait-specific actors from pleiotropic components of the genetic network governing lymphocyte differentiation. Previously, three of the four members of the DNA polymerase X family have been implicated in the junctional diversification process during the somatic assembly of antigen receptors. Our phylogenetic analysis indicates that the presence of terminal deoxynucleotidyl transferase is strictly associated with the facility of V(D)J recombination, whereas PolL and PolM genes are retained even in species lacking Rag-mediated somatic diversification of antigen receptor genes.


Assuntos
Redes Reguladoras de Genes , Linfócitos , Animais , Filogenia , Recombinação V(D)J
4.
Nature ; 544(7651): 427-433, 2017 04 26.
Artigo em Inglês | MEDLINE | ID: mdl-28447635

RESUMO

Cereal grasses of the Triticeae tribe have been the major food source in temperate regions since the dawn of agriculture. Their large genomes are characterized by a high content of repetitive elements and large pericentromeric regions that are virtually devoid of meiotic recombination. Here we present a high-quality reference genome assembly for barley (Hordeum vulgare L.). We use chromosome conformation capture mapping to derive the linear order of sequences across the pericentromeric space and to investigate the spatial organization of chromatin in the nucleus at megabase resolution. The composition of genes and repetitive elements differs between distal and proximal regions. Gene family analyses reveal lineage-specific duplications of genes involved in the transport of nutrients to developing seeds and the mobilization of carbohydrates in grains. We demonstrate the importance of the barley reference sequence for breeding by inspecting the genomic partitioning of sequence variation in modern elite germplasm, highlighting regions vulnerable to genetic erosion.


Assuntos
Cromossomos de Plantas/genética , Genoma de Planta/genética , Hordeum/genética , Núcleo Celular/genética , Centrômero/genética , Cromatina/genética , Cromatina/metabolismo , Mapeamento Cromossômico , Cromossomos Artificiais Bacterianos/genética , Variação Genética , Genômica , Haplótipos/genética , Meiose/genética , Sequências Repetitivas de Ácido Nucleico/genética , Sementes/genética
5.
BMC Genomics ; 17(1): 953, 2016 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-27875982

RESUMO

BACKGROUND: The Rhynchosporium species complex consists of hemibiotrophic fungal pathogens specialized to different sweet grass species including the cereal crops barley and rye. A sexual stage has not been described, but several lines of evidence suggest the occurrence of sexual reproduction. Therefore, a comparative genomics approach was carried out to disclose the evolutionary relationship of the species and to identify genes demonstrating the potential for a sexual cycle. Furthermore, due to the evolutionary very young age of the five species currently known, this genus appears to be well-suited to address the question at the molecular level of how pathogenic fungi adapt to their hosts. RESULTS: The genomes of the different Rhynchosporium species were sequenced, assembled and annotated using ab initio gene predictors trained on several fungal genomes as well as on Rhynchosporium expressed sequence tags. Structures of the rDNA regions and genome-wide single nucleotide polymorphisms provided a hypothesis for intra-genus evolution. Homology screening detected core meiotic genes along with most genes crucial for sexual recombination in ascomycete fungi. In addition, a large number of cell wall-degrading enzymes that is characteristic for hemibiotrophic and necrotrophic fungi infecting monocotyledonous hosts were found. Furthermore, the Rhynchosporium genomes carry a repertoire of genes coding for polyketide synthases and non-ribosomal peptide synthetases. Several of these genes are missing from the genome of the closest sequenced relative, the poplar pathogen Marssonina brunnea, and are possibly involved in adaptation to the grass hosts. Most importantly, six species-specific genes coding for protein effectors were identified in R. commune. Their deletion yielded mutants that grew more vigorously in planta than the wild type. CONCLUSION: Both cryptic sexuality and secondary metabolites may have contributed to host adaptation. Most importantly, however, the growth-retarding activity of the species-specific effectors suggests that host adaptation of R. commune aims at extending the biotrophic stage at the expense of the necrotrophic stage of pathogenesis. Like other apoplastic fungi Rhynchosporium colonizes the intercellular matrix of host leaves relatively slowly without causing symptoms, reminiscent of the development of endophytic fungi. Rhynchosporium may therefore become an object for studying the mutualism-parasitism transition.


Assuntos
Ascomicetos/classificação , Ascomicetos/genética , Genoma Fúngico , Genômica , Especificidade de Hospedeiro , Filogenia , Poaceae/microbiologia , Sequência de Aminoácidos , Ascomicetos/metabolismo , DNA Intergênico , Regulação Fúngica da Expressão Gênica , Genes Fúngicos , Genômica/métodos , Família Multigênica , Metabolismo Secundário/genética
6.
Plant Biotechnol J ; 14(7): 1511-22, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-26801048

RESUMO

Hierarchical shotgun sequencing remains the method of choice for assembling high-quality reference sequences of complex plant genomes. The efficient exploitation of current high-throughput technologies and powerful computational facilities for large-insert clone sequencing necessitates the sequencing and assembly of a large number of clones in parallel. We developed a multiplexed pipeline for shotgun sequencing and assembling individual bacterial artificial chromosomes (BACs) using the Illumina sequencing platform. We illustrate our approach by sequencing 668 barley BACs (Hordeum vulgare L.) in a single Illumina HiSeq 2000 lane. Using a newly designed parallelized computational pipeline, we obtained sequence assemblies of individual BACs that consist, on average, of eight sequence scaffolds and represent >98% of the genomic inserts. Our BAC assemblies are clearly superior to a whole-genome shotgun assembly regarding contiguity, completeness and the representation of the gene space. Our methods may be employed to rapidly obtain high-quality assemblies of a large number of clones to assemble map-based reference sequences of plant and animal species with complex genomes by sequencing along a minimum tiling path.


Assuntos
Cromossomos Artificiais Bacterianos/genética , Genoma de Planta , Hordeum/genética , Análise de Sequência de DNA/métodos , Biotecnologia/métodos
7.
Genome Res ; 22(6): 1098-106, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22434426

RESUMO

Dictyostelium discoideum is an amoebozoa that exists in both a free-living unicellular and a multicellular form. It is situated in a deep branch in the evolutionary tree and is particularly noteworthy in having a very A/T-rich genome. Dictyostelium provides an ideal system to examine the extreme to which nucleotide bias may be employed in organizing promoters, genes, and nucleosomes across a genome. We find that Dictyostelium genes are demarcated precisely at their 5' ends by poly-T tracts and precisely at their 3' ends by poly-A tracts. These tracts are also associated with nucleosome-free regions and are embedded with precisely positioned TATA boxes. Homo- and heteropolymeric tracts of A and T demarcate nucleosome border regions. Together, these findings reveal the presence of a variety of functionally distinct polymeric A/T elements. Strikingly, Dictyostelium chromatin may be organized in di-nucleosome units but is otherwise organized as in animals. This includes a +1 nucleosome in a position that predicts the presence of a paused RNA polymerase II. Indeed, we find a strong phylogenetic relationship between the presence of the NELF pausing factor and positioning of the +1 nucleosome. Pausing and +1 nucleosome positioning may have coevolved in animals.


Assuntos
Cromatina/genética , Dictyostelium/genética , Nucleossomos/genética , Poli A/genética , Poli T/genética , Animais , Genes , Filogenia , Regiões Promotoras Genéticas , RNA Polimerase II/genética , TATA Box/genética , Fatores de Transcrição/genética
8.
Plant Physiol ; 164(1): 412-23, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24243933

RESUMO

Barley (Hordeum vulgare) is an important cereal crop and a model species for Triticeae genomics. To lay the foundation for hierarchical map-based sequencing, a genome-wide physical map of its large and complex 5.1 billion-bp genome was constructed by high-information content fingerprinting of almost 600,000 bacterial artificial chromosomes representing 14-fold haploid genome coverage. The resultant physical map comprises 9,265 contigs with a cumulative size of 4.9 Gb representing 96% of the physical length of the barley genome. The reliability of the map was verified through extensive genetic marker information and the analysis of topological networks of clone overlaps. A minimum tiling path of 66,772 minimally overlapping clones was defined that will serve as a template for hierarchical clone-by-clone map-based shotgun sequencing. We integrated whole-genome shotgun sequence data from the individuals of two mapping populations with published bacterial artificial chromosome survey sequence information to genetically anchor the physical map. This novel approach in combination with the comprehensive whole-genome shotgun sequence data sets allowed us to independently validate and improve a previously reported physical and genetic framework. The resources developed in this study will underpin fine-mapping and cloning of agronomically important genes and the assembly of a draft genome sequence.


Assuntos
Hordeum/genética , Mapeamento Físico do Cromossomo , Polimorfismo de Nucleotídeo Único , Cromossomos Artificiais Bacterianos , Mapeamento de Sequências Contíguas , Reprodutibilidade dos Testes , Análise de Sequência de DNA
9.
Nucleic Acids Res ; 41(Database issue): D692-9, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23193285

RESUMO

Many sequence data repositories can give a quick and easily accessible overview on genomes and their annotations. Less widespread is the possibility to compare related genomes with each other in a common database environment. We have previously described the GenColors database system (http://gencolors.fli-leibniz.de) and its applications to a number of bacterial genomes such as Borrelia, Legionella, Leptospira and Treponema. This system has an emphasis on genome comparison. It combines data from related genomes and provides the user with an extensive set of visualization and analysis tools. Eukaryote genomes are normally larger than prokaryote genomes and thus pose additional challenges for such a system. We have, therefore, adapted GenColors to also handle larger datasets of small eukaryotic genomes and to display eukaryotic gene structures. Further recent developments include whole genome views, genome list options and, for bacterial genome browsers, the display of horizontal gene transfer predictions. Two new GenColors-based databases for two fungal species (http://fgb.fli-leibniz.de) and for four social amoebas (http://sacgb.fli-leibniz.de) were set up. Both new resources open up a single entry point for related genomes for the amoebozoa and fungal research communities and other interested users. Comparative genomics approaches are greatly facilitated by these resources.


Assuntos
Bases de Dados Genéticas , Eucariotos/genética , Genômica , Amebozoários/genética , Genoma Fúngico , Internet , Anotação de Sequência Molecular
10.
Genome Res ; 21(11): 1882-91, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21757610

RESUMO

Dictyostelium discoideum (DD), an extensively studied model organism for cell and developmental biology, belongs to the most derived group 4 of social amoebas, a clade of altruistic multicellular organisms. To understand genome evolution over long time periods and the genetic basis of social evolution, we sequenced the genomes of Dictyostelium fasciculatum (DF) and Polysphondylium pallidum (PP), which represent the early diverging groups 1 and 2, respectively. In contrast to DD, PP and DF have conventional telomere organization and strongly reduced numbers of transposable elements. The number of protein-coding genes is similar between species, but only half of them comprise an identifiable set of orthologous genes. In general, genes involved in primary metabolism, cytoskeletal functions and signal transduction are conserved, while genes involved in secondary metabolism, export, and signal perception underwent large differential gene family expansions. This most likely signifies involvement of the conserved set in core cell and developmental mechanisms, and of the diverged set in niche- and species-specific adaptations for defense and food, mate, and kin selection. Phylogenetic dating using a concatenated data set and extensive loss of synteny indicate that DF, PP, and DD split from their last common ancestor at least 0.6 billion years ago.


Assuntos
Dictyostelium/genética , Genoma de Protozoário , Filogenia , Sequência de Aminoácidos/genética , Composição de Bases , Transporte Biológico , Adesão Celular/genética , Comunicação Celular/genética , Movimento Celular/genética , Centrômero/genética , Centrômero/metabolismo , Citoesqueleto/genética , Dictyostelium/metabolismo , Evolução Molecular , Dados de Sequência Molecular , Estrutura Molecular , Nucleotídeos Cíclicos/metabolismo , Fases de Leitura Aberta , Domínios e Motivos de Interação entre Proteínas , Transdução de Sinais , Sintenia , Telômero/genética , Telômero/metabolismo , Transcrição Gênica
11.
Fungal Genet Biol ; 62: 34-42, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24240058

RESUMO

We sequenced and annotated the complete mitochondrial (mt) genomes of four closely related Rhynchosporium species that diverged ∼14,000-35,000years ago. During this time frame, three of the mt genomes expanded significantly due to an invasion of introns into three genes (cox1, cox2, and nad5). The enlarged mt genomes contained ∼40% introns compared to 8.1% in uninvaded relatives. Many intron gains were accompanied by co-conversion of flanking exonic regions. The comparative analysis revealed a highly variable set of non-intronic, free-standing ORFs of unknown function (uORFs). This is consistent with a rapidly evolving accessory compartment in the mt genome of these closely related species. Only one free-standing uORF was shared among all mt genomes analyzed. This uORF had a mutation rate similar to the core mt protein-encoding genes, suggesting conservation of function among the species. The nucleotide composition of the core protein-encoding genes significantly differed from those of introns and uORFs. The mt mutation rate was 77 times higher than the nuclear mutation rate, indicating that the phylogeny inferred from mt genes may better resolve the phylogenetic relationships among closely related Rhynchosporium species than phylogenies inferred from nuclear genes.


Assuntos
Ascomicetos/genética , DNA Mitocondrial/genética , Genoma Mitocondrial , Íntrons , Taxa de Mutação , Filogenia , Análise de Sequência de DNA/métodos
12.
Plant Cell ; 23(4): 1249-63, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21467582

RESUMO

We used a novel approach that incorporated chromosome sorting, next-generation sequencing, array hybridization, and systematic exploitation of conserved synteny with model grasses to assign ~86% of the estimated ~32,000 barley (Hordeum vulgare) genes to individual chromosome arms. Using a series of bioinformatically constructed genome zippers that integrate gene indices of rice (Oryza sativa), sorghum (Sorghum bicolor), and Brachypodium distachyon in a conserved synteny model, we were able to assemble 21,766 barley genes in a putative linear order. We show that the barley (H) genome displays a mosaic of structural similarity to hexaploid bread wheat (Triticum aestivum) A, B, and D subgenomes and that orthologous genes in different grasses exhibit signatures of positive selection in different lineages. We present an ordered, information-rich scaffold of the barley genome that provides a valuable and robust framework for the development of novel strategies in cereal breeding.


Assuntos
Cromossomos de Plantas/genética , Genoma de Planta/genética , Genômica/métodos , Hordeum/genética , Centrômero/genética , Evolução Molecular , Ordem dos Genes/genética , Rearranjo Gênico/genética , Genes de Plantas/genética , Modelos Genéticos , Oryza/genética , Análise de Sequência de DNA , Triticum/genética
13.
BMC Genomics ; 12(1): 243, 2011 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-21592371

RESUMO

BACKGROUND: In highly copy number variable (CNV) regions such as the human defensin gene locus, comprehensive assessment of sequence variations is challenging. PCR approaches are practically restricted to tiny fractions, and next-generation sequencing (NGS) approaches of whole individual genomes e.g. by the 1000 Genomes Project is confined by an affordable sequence depth. Combining target enrichment with NGS may represent a feasible approach. RESULTS: As a proof of principle, we enriched a ~850 kb section comprising the CNV defensin gene cluster DEFB, the invariable DEFA part and 11 control regions from two genomes by sequence capture and sequenced it by 454 technology. 6,651 differences to the human reference genome were found. Comparison to HapMap genotypes revealed sensitivities and specificities in the range of 94% to 99% for the identification of variations.Using error probabilities for rigorous filtering revealed 2,886 unique single nucleotide variations (SNVs) including 358 putative novel ones. DEFB CN determinations by haplotype ratios were in agreement with alternative methods. CONCLUSION: Although currently labor extensive and having high costs, target enriched NGS provides a powerful tool for the comprehensive assessment of SNVs in highly polymorphic CNV regions of individual genomes. Furthermore, it reveals considerable amounts of putative novel variations and simultaneously allows CN estimation.


Assuntos
Cromossomos Humanos Par 8/genética , Variações do Número de Cópias de DNA , Defensinas/genética , Dosagem de Genes , Variação Genética , Sequenciamento de Nucleotídeos em Larga Escala , Família Multigênica , Sequência de Bases , Linhagem Celular , Frequência do Gene , Haplótipos , Humanos , Masculino , Análise de Sequência de DNA
14.
BMC Genomics ; 10: 547, 2009 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-19930547

RESUMO

BACKGROUND: De novo sequencing the entire genome of a large complex plant genome like the one of barley (Hordeum vulgare L.) is a major challenge both in terms of experimental feasibility and costs. The emergence and breathtaking progress of next generation sequencing technologies has put this goal into focus and a clone based strategy combined with the 454/Roche technology is conceivable. RESULTS: To test the feasibility, we sequenced 91 barcoded, pooled, gene containing barley BACs using the GS FLX platform and assembled the sequences under iterative change of parameters. The BAC assemblies were characterized by N50 of approximately 50 kb (N80 approximately 31 kb, N90 approximately 21 kb) and a Q40 of 94%. For approximately 80% of the clones, the best assemblies consisted of less than 10 contigs at 24-fold mean sequence coverage. Moreover we show that gene containing regions seem to assemble completely and uninterrupted thus making the approach suitable for detecting complete and positionally anchored genes.By comparing the assemblies of four clones to their complete reference sequences generated by the Sanger method, we evaluated the distribution, quality and representativeness of the 454 sequences as well as the consistency and reliability of the assemblies. CONCLUSION: The described multiplex 454 sequencing of barcoded BACs leads to sequence consensi highly representative for the clones. Assemblies are correct for the majority of contigs. Though the resolution of complex repetitive structures requires additional experimental efforts, our approach paves the way for a clone based strategy of sequencing the barley genome.


Assuntos
Cromossomos Artificiais Bacterianos/genética , Processamento Eletrônico de Dados , Genes de Plantas/genética , Hordeum/genética , Análise de Sequência de DNA/métodos , Clonagem Molecular , Genômica , Padrões de Referência , Análise de Sequência de DNA/normas
15.
Microbiol Resour Announc ; 8(46)2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31727707

RESUMO

Rhodococcus qingshengii RL1 was isolated from surface-sterilized leaves of Eruca sativa Mill. and shows plant growth-promoting (PGP) properties. The de novo genome assembly consists of one chromosome with 6,253,838 bp and two plasmids with 144,038 bp and 448,745 bp. Many genes could be identified reflecting its PGP potential.

16.
Sci Adv ; 5(4): eaav1110, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-31032404

RESUMO

Dinoflagellates are microbial eukaryotes that have exceptionally large nuclear genomes; however, their organelle genomes are small and fragmented and contain fewer genes than those of other eukaryotes. The genus Amoebophrya (Syndiniales) comprises endoparasites with high genetic diversity that can infect other dinoflagellates, such as those forming harmful algal blooms (e.g., Alexandrium). We sequenced the genome (~100 Mb) of Amoebophrya ceratii to investigate the early evolution of genomic characters in dinoflagellates. The A. ceratii genome encodes almost all essential biosynthetic pathways for self-sustaining cellular metabolism, suggesting a limited dependency on its host. Although dinoflagellates are thought to have descended from a photosynthetic ancestor, A. ceratii appears to have completely lost its plastid and nearly all genes of plastid origin. Functional mitochondria persist in all life stages of A. ceratii, but we found no evidence for the presence of a mitochondrial genome. Instead, all mitochondrial proteins appear to be lost or encoded in the A. ceratii nucleus.


Assuntos
Dinoflagellida/genética , Dinoflagellida/metabolismo , Genoma Mitocondrial , Mitocôndrias/fisiologia , Filogenia , Aerobiose , Núcleo Celular/genética , Análise por Conglomerados , DNA Complementar/metabolismo , Evolução Molecular , Biblioteca Gênica , Genoma , Funções Verossimilhança , Microscopia Confocal , Análise de Sequência de DNA
17.
Curr Biol ; 28(9): 1370-1379.e5, 2018 05 07.
Artigo em Inglês | MEDLINE | ID: mdl-29706511

RESUMO

The latest major group of plants to evolve were the grasses. These became important in the mid-Paleogene about 40 million years ago. During evolution, leaf CO2 uptake and transpirational water loss were optimized by the acquisition of grass-specific stomatal complexes. In contrast to the kidney-shaped guard cells (GCs) typical of the dicots such as Arabidopsis, in the grasses and agronomically important cereals, the GCs are dumbbell shaped and are associated with morphologically distinct subsidiary cells (SCs). We studied the molecular basis of GC action in the major cereal crop barley. Upon feeding ABA to xylem sap of an intact barley leaf, stomata closed in a nitrate-dependent manner. This process was initiated by activation of GC SLAC-type anion channel currents. HvSLAC1 expressed in Xenopus oocytes gave rise to S-type anion currents that increased several-fold upon stimulation with >3 mM nitrate. We identified a tandem amino acid residue motif that within the SLAC1 channels differs fundamentally between monocots and dicots. When the motif of nitrate-insensitive dicot Arabidopsis SLAC1 was replaced by the monocot signature, AtSLAC1 converted into a grass-type like nitrate-sensitive channel. Our work reveals a fundamental difference between monocot and dicot GCs and prompts questions into the selective pressures during evolution that resulted in fundamental changes in the regulation of SLAC1 function.


Assuntos
Hordeum/fisiologia , Nitratos/farmacologia , Proteínas de Plantas/metabolismo , Estômatos de Plantas/fisiologia , Poaceae/fisiologia , Ácido Abscísico/farmacologia , Motivos de Aminoácidos , Animais , Ânions/metabolismo , Arabidopsis/efeitos dos fármacos , Arabidopsis/metabolismo , Arabidopsis/fisiologia , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Evolução Molecular , Hordeum/efeitos dos fármacos , Hordeum/metabolismo , Ativação do Canal Iônico , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Oócitos/citologia , Oócitos/efeitos dos fármacos , Oócitos/fisiologia , Reguladores de Crescimento de Plantas/farmacologia , Proteínas de Plantas/química , Proteínas de Plantas/genética , Estômatos de Plantas/efeitos dos fármacos , Estômatos de Plantas/metabolismo , Poaceae/efeitos dos fármacos , Poaceae/metabolismo , Conformação Proteica , Transdução de Sinais , Transcriptoma , Xenopus laevis/fisiologia
18.
Genome Biol ; 19(1): 104, 2018 08 17.
Artigo em Inglês | MEDLINE | ID: mdl-30115097

RESUMO

BACKGROUND: Recent improvements in DNA sequencing and genome scaffolding have paved the way to generate high-quality de novo assemblies of pseudomolecules representing complete chromosomes of wheat and its wild relatives. These assemblies form the basis to compare the dynamics of wheat genomes on a megabase scale. RESULTS: Here, we provide a comparative sequence analysis of the 700-megabase chromosome 2D between two bread wheat genotypes-the old landrace Chinese Spring and the elite Swiss spring wheat line 'CH Campala Lr22a'. Both chromosomes were assembled into megabase-sized scaffolds. There is a high degree of sequence conservation between the two chromosomes. Analysis of large structural variations reveals four large indels of more than 100 kb. Based on the molecular signatures at the breakpoints, unequal crossing over and double-strand break repair were identified as the molecular mechanisms that caused these indels. Three of the large indels affect copy number of NLRs, a gene family involved in plant immunity. Analysis of SNP density reveals four haploblocks of 4, 8, 9 and 48 Mb with a 35-fold increased SNP density compared to the rest of the chromosome. Gene content across the two chromosomes was highly conserved. Ninety-nine percent of the genic sequences were present in both genotypes and the fraction of unique genes ranged from 0.4 to 0.7%. CONCLUSIONS: This comparative analysis of two high-quality chromosome assemblies enabled a comprehensive assessment of large structural variations and gene content. The insight obtained from this analysis will form the basis of future wheat pan-genome studies.


Assuntos
Cromossomos de Plantas/genética , Genoma de Planta , Análise de Sequência de DNA , Triticum/genética , Pareamento de Bases/genética , Troca Genética , Quebras de DNA de Cadeia Dupla , Variações do Número de Cópias de DNA/genética , Reparo do DNA/genética , Fluxo Gênico , Genes de Plantas , Haplótipos/genética , Família Multigênica , Polimorfismo de Nucleotídeo Único/genética , Deleção de Sequência/genética , Sintenia/genética
19.
Methods Mol Biol ; 395: 75-96, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-17993668

RESUMO

GenColors (gencolors.fli-leibniz.de) is a new web-based software/database system aimed at an improved and accelerated annotation of prokaryotic genomes considering information on related genomes and making extensive use of genome comparison. It offers a seamless integration of data from ongoing sequencing projects and annotated genomic sequences obtained from GenBank. A variety of export/import filters manages an effective data flow from sequence assembly and manipulation programs (e.g., GAP4) to GenColors and back as well as to standard GenBank file(s). The genome comparison tools include best bidirectional hits, gene conservation, syntenies, and gene core sets. Precomputed UniProt matches allow annotation and analysis in an effective manner. In addition to these analysis options, base-specific quality data (coverage and confidence) can also be handled if available. The GenColors system can be used both for annotation purposes in ongoing genome projects and as an analysis tool for finished genomes. GenColors comes in two types, as dedicated genome browsers and as the Jena Prokaryotic Genome Viewer (JPGV). Dedicated genome browsers contain genomic information on a set of related genomes and offer a large number of options for genome comparison. The system has been efficiently used in the genomic sequencing of Borrelia garinii and is currently applied to various ongoing genome projects on Borrelia, Legionella, Escherichia, and Pseudomonas genomes. One of these dedicated browsers, the Spirochetes Genome Browser (sgb.fli-leibniz.de) with Borrelia, Leptospira, and Treponema genomes, is freely accessible. The others will be released after finalization of the corresponding genome projects. JPGV (jpgv.fli-leibniz.de) offers information on almost all finished bacterial genomes, as compared to the dedicated browsers with reduced genome comparison functionality, however. As of January 2006, this viewer includes 632 genomic elements (e.g., chromosomes and plasmids) of 293 species. The system provides versatile quick and advanced search options for all currently known prokaryotic genomes and generates circular and linear genome plots. Gene information sheets contain basic gene information, database search options, and links to external databases. GenColors is also available on request for local installation.


Assuntos
Bases de Dados Genéticas , Genômica , Internet
20.
Sci Data ; 4: 170044, 2017 04 27.
Artigo em Inglês | MEDLINE | ID: mdl-28448065

RESUMO

Barley (Hordeum vulgare L.) is a cereal grass mainly used as animal fodder and raw material for the malting industry. The map-based reference genome sequence of barley cv. 'Morex' was constructed by the International Barley Genome Sequencing Consortium (IBSC) using hierarchical shotgun sequencing. Here, we report the experimental and computational procedures to (i) sequence and assemble more than 80,000 bacterial artificial chromosome (BAC) clones along the minimum tiling path of a genome-wide physical map, (ii) find and validate overlaps between adjacent BACs, (iii) construct 4,265 non-redundant sequence scaffolds representing clusters of overlapping BACs, and (iv) order and orient these BAC clusters along the seven barley chromosomes using positional information provided by dense genetic maps, an optical map and chromosome conformation capture sequencing (Hi-C). Integrative access to these sequence and mapping resources is provided by the barley genome explorer (BARLEX).


Assuntos
Genoma de Planta , Hordeum/genética , Mapeamento Cromossômico , Análise de Sequência
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