RESUMO
The dynamic of accumulation of extracellular lipoteichoic acid (LTA) was studied depending on the growth stage of Bacillus cereus st. 96. A maximum amount of extracellular LTA was detected in the middle of the exponential growth. The quantity of the biopolymer present in the culture medium at the beginning of the stationary growth under conditions of catabolite repression of sporulation and without repression was found to be different. Experimentally increased concentrations of LTA inhibited B. cereus sporulation. Besides, dormant spores of B. cereus st. 96 were found to contain LTA.
Assuntos
Bacillus cereus/metabolismo , Lipopolissacarídeos/biossíntese , Ácidos Teicoicos/biossíntese , Bacillus cereus/crescimento & desenvolvimento , Bacillus cereus/fisiologia , Cromatografia em Gel , Cromatografia em Camada Fina , Meios de Cultura , Fósforo/análise , Esporos Bacterianos/metabolismoRESUMO
A fraction increasing the resistance of resting spores to UV-irradiation and high temperature has been isolated from the culture medium at the stage of B. cereus st. 96 spore initiation. Amino acid analysis, gas chromatography, electrophoresis, and TLC of the products of acidic and alkaline hydrolysis of the isolated fraction demonstrated that the active component of the fraction was the lipoteichoic acid.