Release of bio-active dentine extracellular matrix components by histone deacetylase inhibitors (HDACi).

AIM: To characterize dentine matrix component (DMC) release and smear layer removal by histone deacetylase inhibitors (HDACis). METHODOLOGY: DMCs were extracted from powdered human dentine over 14 days using three HDACis, valproic acid (VPA), trichostatin A (TSA) and suberoylanilide hydroxamic acid (SAHA) and compared with a control extractant, 10% (w/v) EDTA. Protein compositions of the resultant extracts were analysed by 1D-polyacrylamide gel electrophoresis (1D-PAGE), TGF-ß-1 and MMP-9 ELISAs and a high-throughput growth factor antibody array. Dentine discs with a standardized smear layer were prepared from human molars and treated with EDTA (17% w/v), polyacrylic acid (PA) (20% v/v) and the experimental HDACis prior to analysis by scanning electron microscopy. Parametric ELISA data were analysed using one-way anova and Tukey's post hoc test, whilst nonparametric smear layer data were analysed by Kruskal-Wallis test and Mann-Whitney U-test (P < 0.05). RESULTS: HDACis did not remove smear layer in the presence or absence of PA pre-treatment (P ≥ 0.478). 1D-PAGE analysis demonstrated different protein profiles for EDTA and HDACi extracts. All HDACi solutions released TGF-ß-1 although less effectively than EDTA (P < 0.001), whilst MMP-9 was extracted in significantly higher concentration by EDTA and VPA compared with TSA (P < 0.012). Antibody array analysis demonstrated the ability of HDACis to extract a complex cocktail of established/novel growth factors from dentine, albeit significantly less efficiently than EDTA for certain cytokines (TGF-ß-1, PDGF-AA, VEGF-A) and significantly more effectively for others (GDF-15, IGF-1, EGRF-1, NGFR, BDNF, SCF-R). CONCLUSIONS: HDACi release a range of bioactive DMCs that could promote dentine repair processes in vivo; however, they are ineffective at removing smear layer.

Epigenetic modulation of dental pulp stem cells: implications for regenerative endodontics.

Dental pulp stem cells (DPSCs) offer significant potential for use in regenerative endodontics, and therefore, identifying cellular regulators that control stem cell fate is critical to devising novel treatment strategies. Stem cell lineage commitment and differentiation are regulated by an intricate range of host and environmental factors of which epigenetic influence is considered vital. Epigenetic modification of DNA and DNA-associated histone proteins has been demonstrated to control cell phenotype and regulate the renewal and pluripotency of stem cell populations. The activities of the nuclear enzymes, histone deacetylases, are increasingly being recognized as potential targets for pharmacologically inducing stem cell differentiation and dedifferentiation. Depending on cell maturity and niche in vitro, low concentration histone deacetylase inhibitor (HDACi) application can promote dedifferentiation of several post-natal and mouse embryonic stem cell populations and conversely increase differentiation and accelerate mineralization in DPSC populations, whilst animal studies have shown an HDACi-induced increase in stem cell marker expression during organ regeneration. Notably, both HDAC and DNA methyltransferase inhibitors have also been demonstrated to dramatically increase the reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) for use in regenerative therapeutic procedures. As the regulation of cell fate will likely remain the subject of intense future research activity, this review aims to describe the current knowledge relating to stem cell epigenetic modification, focusing on the role of HDACi on alteration of DPSC phenotype, whilst presenting the potential for therapeutic application as part of regenerative endodontic regimens.

Quantifying the strength of a resin-coated dental ceramic.

Resin luting all-ceramic restorations increases clinical performance; however, the strengthening mechanisms are not fully understood. The authors have previously proposed the existence of a resin-ceramic hybrid layer, and the hypothesis tested was that ceramic strength enhancement was conferred by the characteristics of the resin-ceramic hybrid layer. Dentin porcelain discs were polished with a P4000-grade abrasive paper, and half were centrally indented at 9.8 N. Further discs were alumina-air-abraded. Groups of 30 specimens were coated with resin cement thicknesses varying from 0 to 250 +/- 20 microm before bi-axial flexure testing. Following investigation of residual stresses by annealing, regression analysis enabled us to calculate the magnitude of 'actual' strengthening for a theoretical 'zero' thickness of resin cement on each surface texture. Accounting for resin bulk strengthening, resin cement coating significantly increased the mean strength that was attributed to a resin-ceramic hybrid layer sensitive to surface texture.

Modification of the restoration protocol for resin-based composite (RBC) restoratives (conventional and bulk fill) on cuspal movement and microleakage score in molar teeth.

OBJECTIVE: To modify the resin-based composite (RBC) restoration protocol for standardised Class II cavities in third molar teeth restored using conventional RBCs or their bulk fill restorative counterparts. Employing cuspal deflection using a twin channel deflection measuring gauge (during) and microleakage to determine marginal integrity (following) RBC restoration, the modified restoration protocol results were compared with traditional (oblique) restoration of Class II cavities. METHODS: Thirty-two sound third molar teeth, standardised by size and morphology, were subjected to standardised Class II cavity preparations and randomly allocated to four groups. Restorations were placed in conjunction with a universal bonding system and resin restorative materials were irradiated with a light-emitting-diode light-curing-unit. The cumulative buccal and palatal cuspal movements from a twin channel deflection measuring gauge were summed, the restored teeth fatigued thermally prior to immersion in 0.2% basic fuchsin dye for 24h, before sectioning and examination for microleakage. RESULTS: Teeth restored using conventional RBC materials had significantly higher mean total cuspal movement values compared with bulk fill resin restorative restoration (all p<0.0001). Teeth restored with Admira Fusion and Admira Fusion x-tra had significantly the lowest microleakage scores (all p<0.001) compared with Tetric EvoCeram and Tetric EvoCeram Bulk Fill restored teeth. The microleakage scores for the range of RBC materials tested were significantly reduced (all p<0.001) when the modified RBC restoration protocol was employed compared with the traditional Class II restoration technique. SIGNIFICANCE: Modification of the RBC restoration protocol of some conventional RBCs and bulk fill resin restoratives significantly improve bond integrity and could be translated as a validation of the limited clinical studies available on bulk fill materials in the dental literature where Class II cavities perform less well than Class I cavities following extended follow-up. CLINICAL SIGNIFICANCE: The results of the current study add further weight to experimental protocols employing cuspal movement (during) and cervical microleakage (following) RBC restoration of standardised cavities in natural dentition to provide an indication of polymerization shrinkage stress at the tooth/RBC restoration interface in a 'clinically meaningful context'.

Resin elasticity and the strengthening of all-ceramic restorations.

Resin luting of all-ceramic restorations results in increased performance; however, the strengthening mechanism and the role of the mechanical properties of the resin are not fully understood. The hypothesis tested is that ceramic strength enhancement is dependent on the elastic modulus of the resin. Three-point flexural moduli of a flowable, luting, and hybrid composite resin were characterized. Two hundred forty porcelain discs were air-abraded. One group acted as a control, and 3 additional groups were coated with 120 +/- 20 microm of each resin prior to bi-axial flexure testing. All resins significantly increased in mean strength, and the associated strength increase was related to the elastic modulus of the resin (R(2) = 0.9885), so the hypothesis was accepted. The combination of Poisson constraint and the creation of a resin-inter-penetrating layer sensitive to the elastic modulus of the resin may provide an explanation of the strengthening mechanism.

Cuspal movement and microleakage in premolar teeth restored with resin-based filling materials cured using a 'soft-start' polymerisation protocol.

OBJECTIVES: To investigate the effect of polymerisation shrinkage strain of four posterior filling materials on cuspal movement, degree of conversion (DC) and cervical gingival microleakage of mesio-occlusal-distal (MOD) restorations placed incrementally in maxillary premolar teeth using a 'soft-start' polymerisation protocol. METHODS: Forty sound extracted upper premolar teeth were subjected to standardised preparation of a large MOD cavity before restoration. A 'soft-start' polymerisation curing regimen was used and each posterior filling material was placed in eight increments with the appropriate bonding system. A twin channel deflection measuring gauge allowed a measurement of individual cusp deflections at each stage of polymerisation. Restored teeth were thermocycled before immersion in a 0.2% basic fuchsin dye for 24h. After sagittal sectioning of the restored teeth in a mesio-distal plane, the sectioned restorations were examined to assess cervical microleakage. The DC was also assessed using a diffuse-reflectance accessory on a Fourier transform infra-red spectrophotometer. RESULTS: A significant increase in cuspal movement recorded for Z100 (20.06+/-4.71) compared with Filtek Z250 (16.52+/-3.26), P60 (14.23+/-3.71) and Admira (11.11+/-2.47). No significant reduction in cuspal movement was identified when compared with a previous study [6] where a full-intensity standard polymerisation protocol was employed. No significant differences were also identified between the materials when the cervical gingival microleakage scores or DC were examined for the 'soft-start' compared with the standard polymerisation protocol. CONCLUSIONS: Although material type remained a significant factor, the use of a 'soft-start' polymerization compared with a standard curing regime did not offer any significant reduction in associated cuspal movement, DC or gingival microleakage at the cervical dentine cavosurface margin of the cavities restored with the resin-based filling materials.

Investigation of polymerisation shrinkage strain, associated cuspal movement and microleakage of MOD cavities restored incrementally with resin-based composite using an LED light curing unit.

OBJECTIVES: To investigate the polymerisation shrinkage strain, associated cuspal movement, degree of conversion (DC) and cervical gingival microleakage of mesio-occlusal-distal (MOD) cavities restored with four resin-based composite (RBC) filling materials placed incrementally using a light emitting diode (LED) light curing unit (LCU). METHODS: Standardised extensive MOD cavity preparations on extracted teeth were performed on 40 sound upper premolar teeth. Restoration of the teeth involved the placement of RBCs in eight increments with the appropriate bonding system before irradiation using an LED LCU. Buccal and palatal cusp deflections at each stage of polymerisation were recorded using a twin channel deflection measuring gauge. Following restoration, the teeth were thermocycled, immersed in a 0.2% basic fuchsin dye for 24 h, sagittally sectioned and examined for cervical microleakage. The DC was determined using a Fourier transform infra-red (FT-IR) spectrometer. RESULTS: No significantly difference (P=0.677) in cuspal movement was recorded for Z100 (13.1+/-3.2 microm) compared with Filtek Z250 (8.4+/-3.5 microm), P60 (7.3+/-3.8 microm) and Admira (6.7+/-2.7 microm). The LED LCU deflections were compared with a halogen LCU used in a conventional (Fleming GJP, Hall D, Shorthall ACC, Burke FJT. Cuspal movement and microleakage in premolar teeth restored with posterior filling materials of varying reported volumetric shrinkage values. Journal of Dentistry, 2005;33:139-146) and soft-start mode (Fleming GJP, Cara RR, Palin WM, Burke FJT. Cuspal movement and microleakage in premolar teeth restored with posterior filling materials cured using 'soft-start' polymerization. Dental Materials, 2006, , in press) and a significant reduction in cuspal movement was identified for curing type and material type (P<0.001 and P=0.002, respectively). No significant differences were noted between the four RBC materials investigated when the DC or microleakage scores were examined for the LED LCU. SIGNIFICANCE: It would appear that irradiation of RBCs using the LED LCU offered a significant reduction in associated cuspal movement in large MOD cavities. However, the microleakage scores following polymerisation were significantly increased with dye penetration into the pulp chamber from the axial wall evident in teeth restored with the LED LCU.

Cuspal deflection and microleakage in premolar teeth restored with resin-based composites with and without an intermediary flowable layer.

OBJECTIVES: To assess cuspal deflection and cervical enamel microleakage with and without an intermediary flowable RBC layer for the incremental restoration of mesio-occluso-distal (MOD) cavities with two resin-based composites (RBCs). METHODS: Forty sound upper premolar teeth had standardised MOD cavities prepared. Restoration of the teeth involved the placement of the RBCs (Filtek P60 or Filtek Supreme) in eight increments with the appropriate bonding system with and without an intermediary flowable RBC layer (Filtek Flow). Buccal and palatal cusp deflections were recorded post-irradiation using a twin channel deflection measuring gauge. Following restoration, the teeth were thermocycled, immersed in a 0.2% basic fuchsin dye for 24h, sagittally sectioned and examined for cervical enamel microleakage. RESULTS: A significant reduction in cuspal deflection was evident when both RBC materials were used to restore the cavity by employing an intermediary flowable (P<0.001) compared with when no intermediary flowable was utilised. No statistically significant differences were identified in microleakage between the teeth restored with Filtek P60 or Filtek Supreme when an intermediary flowable (Filtek Flow) was employed. CONCLUSIONS: The results of the current study suggest that there was a benefit to the operator in terms of a reduction in cuspal deflection but not from the maintenance of the synergism of the adhesive bond, namely microleakage at the cervical enamel cavosurface margin, when an intermediate layer of a flowable RBC was used under higher elastic modulus RBCs.

The strengthening mechanism of resin cements on porcelain surfaces.

All-ceramic crowns bonded with resin cements have increased performance, and two theories have been proposed. Marquis (1992) suggested that the resin modified defects by crack healing, while Nathanson (1993) proposed that resin polymerization shrinkage strengthened porcelains. Both theories imply a sensitivity of strengthening to defect size. The hypothesis tested was that resin strength enhancement is independent of defect severity. We ground 200 porcelain discs to remove imperfections and indented 120 to create a large defect. Discs were tested dry, wet, and after being coated with 75-100 microm of resin cement in bi-axial flexure. Disc strength with and without indentations was increased significantly when coated with 2 resin cements. Both cements significantly increased the strength independent of defect population, and the hypothesis was accepted. It is proposed that the combination of surface pre-treatment and cement moved the fracture origin from the porcelain/cement interface to the cement surface, consistent with resin strength enhancement independent of defect severity.

Effectiveness of a self-adhesive resin luting system on fracture resistance of teeth restored with dentin-bonded crowns.

Laboratory studies have demonstrated satisfactory fracture resistance of all-ceramic crowns placed using a conventional resin-composite luting material and a dentine bonding system. This study investigated the fracture resistance of teeth restored with dentine-bonded ceramic crowns luted with a self-etching luting material. Standardized preparations were carried out on two groups of ten sound, unrestored, maxillary premolar teeth. Ceramic crowns were constructed, their internal surfaces etched and placed using two luting system combinations. Compressive fracture resistance was determined for each group using a Universal Testing Machine. Mean compressive fracture resistance of 890 (222) N and 760 (271) N were recorded for the RelyX Unicem Aplicap and Mirage groups, respectively. There was no significant difference in the mean compressive fracture resistance of the restored teeth in both groups (P<0.05). The failure modes were different for each cements with more severe (Mode V)failures associated with the Mirage group compared with the consistent Mode II failures associated with RelyX Unicem Aplicap. We conclude that the fracture resistance of dentine-bonded crowns luted with a self-adhesive resin-based luting material was no different from that of a conventional resin-based luting system. The fracture resistance of dentine-bonded ceramic crowns may be clinically satisfactory when a self-adhesive resin-based luting material is utilised.

The reliability of standardized flexure strength testing procedures for a light-activated resin-based composite.

OBJECTIVES: To investigate the differences in the reliability of three-point flexure strength (TFS) and bi-axial flexure strength (BFS) data of a dental resin-based composite (RBC) irradiated by a hand-held or an oven light-curing unit (LCU). METHODS: Three-point bar-shaped (25 x 2 x 2 mm3) and bi-axial disc-shaped (12 mm diameter, 2 mm thick) specimens of Filtek Z250 were polymerized utilizing either a hand-held (n = 20) or an oven-LCU (n = 20). The mean TFS and BFS, associated Weibull moduli and degree of conversion (DC) for each curing regime were obtained following 24 h immersion in a light-proof water bath maintained at 37 +/-1 degrees C. RESULTS: A significant decrease in TFS (129 +/- 15 and 127 +/- 13 MPa) compared with BFS (140 +/- 12 and 148 +/- 13 MPa) was identified for specimens irradiated with both LCU types (P < 0.001). The Weibull moduli of TFS data associated with the hand-held--was significantly decreased compared with the oven-LCU since confidence intervals did not overlap (7.5-9.4 and 9.5-10.6, respectively). In contrast, the Weibull moduli of the BFS data associated with either LCU were not significant (11.3-12.4 and 11.3-13.5). A significant decrease in the DC of three-point and bi-axial flexure specimens irradiated with the hand-held compared with the oven-LCU was reported (P = 0.031). CONCLUSIONS: The improved experimental reliability combined with the increased clinical relevance in specimen geometry of disc-compared with bar-shaped specimens may advocate bi-axial flexure testing methodology as the standard to assess the strength of light-activated dental RBCs. The differences in extent of polymerization of RBC specimens cured with either LCU were not consistent with an equivalent dose of light energy density. This phenomenon may be attributed to differences in polymerization efficiency associated with the quantity of useful light energy emitted from the hand-held--compared with the oven-LCU.

The frictional coefficients and associated wear resistance of novel low-shrink resin-based composites.

OBJECTIVES: Frictional forces play a major role in the oral wear process of dental resin-based composites (RBCs) and it would be of interest to consider how the energy from friction is dissipated at the material surface. Consequently, the micromechanical wear properties of conventional methacrylate compared with novel oxirane RBCs were assessed. METHOD: The frictional coefficient (mu), volume loss and Vickers hardness number (VHN) of oxirane (EXL596 and H1) and methacrylate RBCs (Z100 and Filtek Z250) were evaluated. Archard's wear equation was implemented to obtain the wear coefficient (K) and expressed as a 'fraction of friction' (K/micro) to indicate the dissipation of frictional energy that resulted in wear. Scanning electron microscopy (SEM) was used to qualitatively asses the wear facets of each RBC following 50000-cycles. RESULTS: The mean frictional coefficients observed between the oxirane and methacrylate RBCs were not significantly different (P > 0.05). However, the volume loss of EXL596 and H1 (5.9 +/- 0.4 and 4.7 +/- 0.3 x 10(-2) mm(3)) was significantly increased compared with Z100 and Filtek Z250 (1.7 +/- 0.2 and 2.3 +/- 0.3 x 10(-2) mm(3)). The VHN of EXL596 and H1 was either significantly greater (P = 0.021) or similar (P = 0.089) to Filtek Z250, respectively. An increase in K/micro was reported for EXL596 and H1 (34.7 +/- 4.1 and 22.8+ /- 2.4 x 10(-4)) compared with Z100 and Filtek Z250 (8.50 +/- 0.7 x 10(-4) and 8.62 +/- 1.0 x 10(-4)) (P < 0.05). SEM images of the oxirane RBCs exhibited increased surface fatigue and delamination of the surface layers compared with the methacrylate RBC specimens following 50,000-cycles. CONCLUSION: The significant decrease in wear resistance of the oxirane compared with methacrylate RBCs was unexpected since frictional coefficients and/or surface hardness were statistically similar. The decreased wear resistance of EXL596 and H1 compared with Z100 and Filtek Z250 was further explained by the increase in K/micro from wear theory and the associated increase in surface fatigue identified from SEM. The simplistic testing procedure combined with SEM utilized in the current investigation provided a greater insight into the wear mechanism by considering the effect of frictional energy at the specimen surface which may benefit the development of improved wear resistance for experimental RBC materials.

The influence of short and medium-term water immersion on the hydrolytic stability of novel low-shrink dental composites.

OBJECTIVES: Differences in mechanical and physical properties exhibited by novel low-shrink resin-based composite (RBC) formulations compared with conventional methacrylate RBCs may contribute to the clinical success of the candidate material. The aim of the current study was to investigate the effect of water uptake characteristics and water solubility on the mechanical properties of two methacrylate (Z100 and Filtek Z250), an experimental oxirane (OXI) and silorane (SIL) RBC following short- and medium-term immersion. METHODS: The water sorption/solubility and associated diffusion coefficients of each material (n=5) were measured using gravimetric analysis following short- (0.1, 0.5, 1, 4, 24 and 48 h) and medium-term (1, 4, 12 and 26w) immersion. The bi-axial flexure strength, associated Weibull moduli (n=20) and fracture analysis using scanning electron microscopy (SEM) of each material for similar immersion periods was also investigated. RESULTS: Following 0.5h and each subsequent short- and medium-term immersion period the water sorption of Z100 and Filtek Z250 was decreased compared with OXI. A significant decrease in bi-axial flexure strength and associated increase in filler particle exfoliation identified through SEM was identified for OXI compared with Z100, Filtek Z250 and SIL following 26w immersion. SIL exhibited the significantly lowest water sorption, solubility and associated diffusion coefficient following each immersion period. SIGNIFICANCE: The increase in water sorption, solubility and the associated diffusion coefficient of the experimental oxirane RBC, OXI was manifested as a significant decrease in bi-axial flexure strength and attributed to the decrease in synergy between the filler particles and resin matrix. The decreased water sorption, solubility and associated diffusion coefficient of the experimental silorane RBC, SIL may potentially improve hydrolytic stability of RBC restorations demonstrated by the non-significant decrease in bi-axial flexure strength following medium-term immersion.

Cuspal movement and microleakage in premolar teeth restored with posterior filling materials of varying reported volumetric shrinkage values.

OBJECTIVES: To investigate the effect of polymerisation shrinkage stress of various aesthetic posterior filling materials on cuspal movement and cervical gingival microleakage of mesio-occlusal-distal (MOD) restorations placed in increments in extracted maxillary premolar teeth. METHODS: Forty sound extracted upper premolar teeth were subjected to standardised preparation of a large MOD cavity. One curing regimen was used and each posterior filling material was placed in eight increments with the appropriate bonding system. A twin channel deflection-measuring gauge allowed a measurement of individual cusp deflections at each stage of polymerisation. Restored teeth were thermocycled before immersion in 0.2% basic fuchsin dye for 24h. After sagittal sectioning of the restored teeth in a mesio-distal plane, the sectioned restorations were examined to assess cervical gingival microleakage. RESULTS: In general, cuspal deflection measurements were dependent upon the constituent monomers and the associated shrinkage on curing, with significantly increased cuspal movement (P<0.05) being recorded for Z100 (20.03+/-2.92 microm) compared with Filtek Z250 (12.34+/-2.18 microm), P60 (13.41+/-4.43 microm) and Admira (11.2+/-2.58 microm). No significant differences were identified between the posterior filling materials when the cervical gingival microleakage scores were examined. CONCLUSIONS: It would appear that a reduction in the manufacturers' reported volumetric polymerisation shrinkage for Z100 (4.0%) to below 3% for Filtek Z250, P60 and Admira, resulted in a significant reduction in the associated cuspal strain on the MOD cavity. The diluent triethyleneglycol dimethacrylate (TEGDMA) increases the polymerisation shrinkage of Z100 resin-based composite due to an increased concentration of carbon-to-carbon double bonds (CC). The replacement of TEGDMA with urethane dimethacrylate (UDMA) and Bis-EMA in Filtek Z250 and P60, decreases the polymerisation shrinkage stress by increasing the cross-linking of polymer networks. However, no group was identified as producing less gingival microleakage at the cervical dentine cavosurface margin when the cavities were sectioned and examined, regardless of the reported variations in cuspal strain and the associated volumetric polymerisation shrinkage values.

In vitro ageing of brushite calcium phosphate cement.

In vivo studies investigating the use of brushite cements have demonstrated mixed results with one or more of dissolution, hydrolysis, fragmentation and long term stability being demonstrated. It has been suggested that sample volume, implant location, and species can affect in vivo behaviour. As few in vitro studies on this cement system have been performed, this study aimed to compare the effects of static and dynamic in vitro ageing protocols on the phase composition, weight loss and mechanical properties of brushite cement. The effects of immersion liquid to cement volume ratio (LCVR) and sample volume on phase composition were investigated and comparative in vitro experiments were also performed in foetal bovine serum. It was determined that the weight loss after 28 days was up to seven times higher in serum than in phosphate buffered saline (PBS) and that fragmentation accounted for most of the weight loss observed. Hydroxyapatite was formed in PBS but not in serum when aged in refreshed media at all LCVRs investigated. This study has highlighted that LCVR, media refresh rate and media composition are critical to brushite cement performance. It appears that brushite cement removal from an implant site may be complex and dependent on physiological processes other than simple dissolution. A better understanding of these processes could provide the means to engineer more precise calcium phosphate cement degradation profiles.

Cuspal movement and microleakage in premolar teeth restored with a packable composite cured in bulk or in increments.

INTRODUCTION: The aim of this study was to investigate the effect of two novel curing systems (a plasma arc light, and a 'turbo-boosted' conventional curing light) on cuspal movement and gingival microleakage of 'packable' resin-based composite (RBC) restorations placed in extracted maxillary premolar teeth. MATERIALS AND METHODS: Forty sound extracted upper premolar teeth were subjected to standardised preparation of a large mesio-occlusal-distal cavity before restoration with a RBC. Four curing regimens were used. Either the RBC was placed in bulk and light-cured in one increment using (a). the plasma arc light; (b). the 'turbo-boosted' curing light, or the RBC was placed in eight increments using (c). the plasma arc light; (d). the 'turbo-boosted' curing light. A deflection measuring gauge allowed a measurement of cuspal deflection at each stage of polymerisation. Restored teeth were thermocycled before immersion in a 0.2% basic fuchsin dye for 24 h. After sagittal sectioning of the restored teeth in a mesio-distal plane, the sectioned restorations were examined to assess cervical microleakage. RESULTS: Cuspal deflection measurements were significantly increased when the 'turbo-boosted' halogen curing light was compared with the plasma arc light. Total mean cuspal deflection measurements obtained with incremental cure were significantly increased compared with bulk cure for both light sources. Gingival microleakage for bulk restored teeth was significantly increased compared with teeth restored incrementally. Incremental restoration with the plasma arc light had significantly increased gingival microleakage compared with the 'turbo-boosted' halogen curing light. CONCLUSIONS: The packable composite tested could not be cured adequately to a depth of 5 mm with the plasma arc light within the specified irradiation time. Under the test conditions of the current investigation, bulk curing only appeared to be practical with the high intensity halogen light (40 s activation). Incremental build-up and polymerisation optimised marginal seal for the high intensity halogen light but led to greater cuspal deflection.

A preliminary report on the incidence of pre-existing pinhole defects in nitrile dental gloves.

INTRODUCTION: Examination gloves manufactured from natural latex have been the predominant glove choice to date in dental practice. However, concerns over hypersensitivity have resulted in the use of alternatives such as nitrile gloves. The aim of the current study was to assess the incidence of pre-existing pinhole defects in nitrile examination gloves. METHODS: Air inflation, followed by water submersion, was used to assess the incidence of pre-existing pinhole defects in five nitrile and two latex glove types. The gloves were filled with a constant volume of air and submerged in 3 litres of water for 10 seconds while being observed for air bubbles which would indicate pinhole defects. The position and number of pinholes were noted for 100 gloves of each type investigated. RESULTS: The incidence of pre-existing pinholes for latex gloves was 0% for the non-sterile surgical latex glove type and 3% for the powdered latex examination glove type, with pinholes located on the thumb, middle finger and ring finger. Of the nitrile gloves evaluated, three types were assessed to have no pre-existing pinhole defects. One type had a 2% incidence of pre-existing pinhole defects--one pinhole located on the thumb region of the glove and one on the ring finger portion of the glove. The fifth nitrile glove type had one pre-existing pinhole defect located on the middle finger. SIGNIFICANCE: All glove types examined met the European Standard (EN 455-1) and there was no statistically significant difference between glove types. However, the nitrile gloves generally exhibited less pre-existing pinhole defects than the latex examination gloves.

Puncture resistance and stiffness of nitrile and latex dental examination gloves.

OBJECTIVE: The aim of the current study was to assess the puncture resistance and stiffness of nitrile and latex dental examination gloves. METHODS: Puncture resistance was measured by employing an adapted version of ASTM F1342-91 using both a 316 stainless steel puncture probe (0.8 mm diameter) and a dental injection needle (0.45 mm diameter) interfaced to a tensile testing apparatus. Glove specimens (12 cm length, 1.5 cm breadth) were removed for modulus (M100) evaluation by assessing the force required to elongate the specimen to 100% of the original length. Glove samples were also aged to investigate whether puncture resistance and M100 values varied with aging at 70 degrees C for 7 days in an air-circulating oven. RESULTS: The nitrile glove types were assessed to have significantly higher puncture resistance compared with the latex glove type when the steel puncture probe was the pentrometer when using the one way analysis of variance (ANOVA) at the 95% significance level. Interestingly the puncture resistance for the latex glove type was significantly higher (P < 0.001) when a dental injection needle was used as the pentrometer compared with the nitrile glove types. The M100 values were significantly higher for the nitrile glove types for which the stiffness increased when the gloves were aged (P < 0.001). CONCLUSIONS: The higher stiffness values resulted in increased puncture resistance when the nitrile glove specimens were aged irrespective of the pentrometer type. However, the ability of latex to re-seal itself on puncture may be beneficial when considering the protection potential of each glove type against breaches in cross infection. For clinicians that have experienced an adverse reaction to natural latex gloves, the results of the current study indicate that nitrile gloves are available at reasonable cost and offer the clinician comparable resistance to puncture with latex gloves.

Low-shrink monomers for dental restorations.

The main disadvantages of resin-based composites (RBCs) for use in load-bearing posterior restorations include the polymerization shrinkage following curing and inadequate wear resistance in service. These properties are largely influenced by the monomer system and research is currently being undertaken to decrease polymerization shrinkage and improve resin wear characteristics in an attempt to increase RBC restoration longevity. The scope of the current review will identify the development of resin-based restoratives, indicating the reported advantages and disadvantages of resin types routinely used in dental practice today and review the most recent advancements in resin technology.

Materials for restoration of primary teeth: 2. Glass ionomer derivatives and compomers.

A wide variety of materials have been used for the restoration of primary teeth. Resin-modified glass ionomers and the more recently introduced viscous glass ionomers have been developed from conventional glass ionomer materials in an attempt to overcome the suboptimal properties of conventional glass ionomers. These materials would appear to have the necessary physical characteristics for restorations in load-bearing situations in primary teeth, as have the resin-based compomer materials, which now have well documented success rates in a number of studies. The first of these two articles described 'traditional' restorative materials, including amalgam and conventional glass ionomer, for the restoration of primary teeth. This paper describes materials derived from traditional glass ionomers in an attempt to overcome the suboptimal properties of conventional glass ionomers and resin-based materials such as compomer.