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1.
Molecules ; 27(21)2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36364451

RESUMO

Faced with new and as yet unmet medical need, the stark underperformance of the pharmaceutical discovery process is well described if not perfectly understood. Driven primarily by profit rather than societal need, the search for new pharmaceutical products-small molecule drugs, biologicals, and vaccines-is neither properly funded nor sufficiently systematic. Many innovative approaches remain significantly underused and severely underappreciated, while dominant methodologies are replete with problems and limitations. Design is a component of drug discovery that is much discussed but seldom realised. In and of itself, technical innovation alone is unlikely to fulfil all the possibilities of drug discovery if the necessary underlying infrastructure remains unaltered. A fundamental revision in attitudes, with greater reliance on design powered by computational approaches, as well as a move away from the commercial imperative, is thus essential to capitalise fully on the potential of pharmaceutical intervention in healthcare.


Assuntos
Produtos Biológicos , Vacinas , Indústria Farmacêutica/métodos , Descoberta de Drogas , Preparações Farmacêuticas , Desenho de Fármacos
2.
BMC Bioinformatics ; 21(1): 116, 2020 03 19.
Artigo em Inglês | MEDLINE | ID: mdl-32192427

RESUMO

After publication of the original article [1], we were notified that legends of Fig. 1 and Fig. 2 have been swapped.

3.
BMC Bioinformatics ; 20(Suppl 6): 476, 2019 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-31823715

RESUMO

BACKGROUND: Human Cytomegalovirus (HCMV) is a ubiquitous herpesvirus affecting approximately 90% of the world population. HCMV causes disease in immunologically naive and immunosuppressed patients. The prevention, diagnosis and therapy of HCMV infection are thus crucial to public health. The availability of effective prophylactic and therapeutic treatments remain a significant challenge and no vaccine is currently available. Here, we sought to define an epitope-based vaccine against HCMV, eliciting B and T cell responses, from experimentally defined HCMV-specific epitopes. RESULTS: We selected 398 and 790 experimentally validated HCMV-specific B and T cell epitopes, respectively, from available epitope resources and apply a knowledge-based approach in combination with immunoinformatic predictions to ensemble a universal vaccine against HCMV. The T cell component consists of 6 CD8 and 6 CD4 T cell epitopes that are conserved among HCMV strains. All CD8 T cell epitopes were reported to induce cytotoxic activity, are derived from early expressed genes and are predicted to provide population protection coverage over 97%. The CD4 T cell epitopes are derived from HCMV structural proteins and provide a population protection coverage over 92%. The B cell component consists of just 3 B cell epitopes from the ectodomain of glycoproteins L and H that are highly flexible and exposed to the solvent. CONCLUSIONS: We have defined a multiantigenic epitope vaccine ensemble against the HCMV that should elicit T and B cell responses in the entire population. Importantly, although we arrived to this epitope ensemble with the help of computational predictions, the actual epitopes are not predicted but are known to be immunogenic.


Assuntos
Biologia Computacional/métodos , Vacinas contra Citomegalovirus , Citomegalovirus/imunologia , Epitopos/imunologia , Humanos
4.
BMC Immunol ; 19(1): 11, 2018 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-29544447

RESUMO

Cancer kills 8 million annually worldwide. Although survival rates in prevalent cancers continue to increase, many cancers have no effective treatment, prompting the search for new and improved protocols. Immunotherapy is a new and exciting addition to the anti-cancer arsenal. The successful and accurate identification of aberrant host proteins acting as antigens for vaccination and immunotherapy is a key aspiration for both experimental and computational research. Here we describe key elements of in silico prediction, including databases of cancer antigens and bleeding-edge methodology for their prediction. We also highlight the role dendritic cell vaccines can play and how they can act as delivery mechanisms for epitope ensemble vaccines. Immunoinformatics can help streamline the discovery and utility of Cancer Immunogens.


Assuntos
Antígenos de Neoplasias/imunologia , Vacinas Anticâncer/imunologia , Simulação por Computador , Fatores Imunológicos/imunologia , Neoplasias/imunologia , Antígenos de Neoplasias/uso terapêutico , Vacinas Anticâncer/uso terapêutico , Ensaios Clínicos como Assunto , Biologia Computacional/métodos , Células Dendríticas/imunologia , Humanos , Fatores Imunológicos/uso terapêutico , Imunoterapia/métodos , Neoplasias/terapia
5.
Bioinformatics ; 32(21): 3233-3239, 2016 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-27402904

RESUMO

MOTIVATION: Influenza A viral heterogeneity remains a significant threat due to unpredictable antigenic drift in seasonal influenza and antigenic shifts caused by the emergence of novel subtypes. Annual review of multivalent influenza vaccines targets strains of influenza A and B likely to be predominant in future influenza seasons. This does not induce broad, cross protective immunity against emergent subtypes. Better strategies are needed to prevent future pandemics. Cross-protection can be achieved by activating CD8+ and CD4+ T cells against highly conserved regions of the influenza genome. We combine available experimental data with informatics-based immunological predictions to help design vaccines potentially able to induce cross-protective T-cells against multiple influenza subtypes. RESULTS: To exemplify our approach we designed two epitope ensemble vaccines comprising highly conserved and experimentally verified immunogenic influenza A epitopes as putative non-seasonal influenza vaccines; one specifically targets the US population and the other is a universal vaccine. The USA-specific vaccine comprised 6 CD8+ T cell epitopes (GILGFVFTL, FMYSDFHFI, GMDPRMCSL, SVKEKDMTK, FYIQMCTEL, DTVNRTHQY) and 3 CD4+ epitopes (KGILGFVFTLTVPSE, EYIMKGVYINTALLN, ILGFVFTLTVPSERG). The universal vaccine comprised 8 CD8+ epitopes: (FMYSDFHFI, GILGFVFTL, ILRGSVAHK, FYIQMCTEL, ILKGKFQTA, YYLEKANKI, VSDGGPNLY, YSHGTGTGY) and the same 3 CD4+ epitopes. Our USA-specific vaccine has a population protection coverage (portion of the population potentially responsive to one or more component epitopes of the vaccine, PPC) of over 96 and 95% coverage of observed influenza subtypes. The universal vaccine has a PPC value of over 97 and 88% coverage of observed subtypes. AVAILABILITY AND IMPLEMENTATION: http://imed.med.ucm.es/Tools/episopt.html CONTACT: d.r.flower@aston.ac.uk.


Assuntos
Simulação por Computador , Vírus da Influenza A/imunologia , Vacinas contra Influenza , Influenza Humana/prevenção & controle , Linfócitos T CD4-Positivos , Epitopos de Linfócito T , Humanos , Imunogenética , Influenza Humana/imunologia
6.
Bioinformatics ; 32(6): 821-7, 2016 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-26568629

RESUMO

MOTIVATION: In any macromolecular polyprotic system-for example protein, DNA or RNA-the isoelectric point-commonly referred to as the pI-can be defined as the point of singularity in a titration curve, corresponding to the solution pH value at which the net overall surface charge-and thus the electrophoretic mobility-of the ampholyte sums to zero. Different modern analytical biochemistry and proteomics methods depend on the isoelectric point as a principal feature for protein and peptide characterization. Protein separation by isoelectric point is a critical part of 2-D gel electrophoresis, a key precursor of proteomics, where discrete spots can be digested in-gel, and proteins subsequently identified by analytical mass spectrometry. Peptide fractionation according to their pI is also widely used in current proteomics sample preparation procedures previous to the LC-MS/MS analysis. Therefore accurate theoretical prediction of pI would expedite such analysis. While such pI calculation is widely used, it remains largely untested, motivating our efforts to benchmark pI prediction methods. RESULTS: Using data from the database PIP-DB and one publically available dataset as our reference gold standard, we have undertaken the benchmarking of pI calculation methods. We find that methods vary in their accuracy and are highly sensitive to the choice of basis set. The machine-learning algorithms, especially the SVM-based algorithm, showed a superior performance when studying peptide mixtures. In general, learning-based pI prediction methods (such as Cofactor, SVM and Branca) require a large training dataset and their resulting performance will strongly depend of the quality of that data. In contrast with Iterative methods, machine-learning algorithms have the advantage of being able to add new features to improve the accuracy of prediction. CONTACT: yperez@ebi.ac.uk AVAILABILITY AND IMPLEMENTATION: The software and data are freely available at https://github.com/ypriverol/pIRSupplementary information: Supplementary data are available at Bioinformatics online.


Assuntos
Sequência de Aminoácidos , Focalização Isoelétrica , Ponto Isoelétrico , Peptídeos , Proteômica , Espectrometria de Massas em Tandem
7.
J Theor Biol ; 430: 109-116, 2017 10 07.
Artigo em Inglês | MEDLINE | ID: mdl-28716385

RESUMO

Linguistic analysis of protein sequences is an underexploited technique. Here, we capitalize on the concept of the lipogram to characterize sequences at the proteome levels. A lipogram is a literary composition which omits one or more letters. A protein lipogram likewise omits one or more types of amino acid. In this article, we establish a usable terminology for the decomposition of a sequence collection in terms of the lipogram. Next, we characterize Uniref50 using a lipogram decomposition. At the global level, protein lipograms exhibit power-law properties. A clear correlation with metabolic cost is seen. Finally, we use the lipogram construction to assign proteomes to the four branches of the tree-of-life: archaea, bacteria, eukaryotes and viruses. We conclude from this pilot study that the lipogram demonstrates considerable potential as an additional tool for sequence analysis and proteome classification.


Assuntos
Sequência de Aminoácidos , Proteínas/química , Proteoma/classificação , Archaea , Bactérias , Eucariotos , Evolução Molecular , Projetos Piloto , Vírus
8.
Bioinformatics ; 31(15): 2469-74, 2015 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-25810434

RESUMO

MOTIVATION: Within bioinformatics, the textual alignment of amino acid sequences has long dominated the determination of similarity between proteins, with all that implies for shared structure, function and evolutionary descent. Despite the relative success of modern-day sequence alignment algorithms, so-called alignment-free approaches offer a complementary means of determining and expressing similarity, with potential benefits in certain key applications, such as regression analysis of protein structure-function studies, where alignment-base similarity has performed poorly. RESULTS: Here, we offer a fresh, statistical physics-based perspective focusing on the question of alignment-free comparison, in the process adapting results from 'first passage probability distribution' to summarize statistics of ensemble averaged amino acid propensity values. In this article, we introduce and elaborate this approach.


Assuntos
Análise de Sequência de Proteína/métodos , Algoritmos , Interpretação Estatística de Dados , Física , Alinhamento de Sequência
9.
Bioinformatics ; 31(2): 295-6, 2015 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-25252779

RESUMO

UNLABELLED: A protein's isoelectric point or pI corresponds to the solution pH at which its net surface charge is zero. Since the early days of solution biochemistry, the pI has been recorded and reported, and thus literature reports of pI abound. The Protein Isoelectric Point database (PIP-DB) has collected and collated these data to provide an increasingly comprehensive database for comparison and benchmarking purposes. A web application has been developed to warehouse this database and provide public access to this unique resource. PIP-DB is a web-enabled SQL database with an HTML GUI front-end. PIP-DB is fully searchable across a range of properties. AVAILABILITY AND IMPLEMENTATION: The PIP-DB database and documentation are available at http://www.pip-db.org.


Assuntos
Bases de Dados de Proteínas , Proteínas/química , Software , Ponto Isoelétrico
10.
Nat Chem Biol ; 9(12): 749-53, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24231606

RESUMO

Peptides fulfill many roles in immunology, yet none are more important than their role as immunogenic epitopes driving the adaptive immune response, our ultimate bulwark against infectious disease. Peptide epitopes are mediated primarily by their interaction with major histocompatibility complexes (T-cell epitopes) and antibodies (B-cell epitopes). As pathogen genomes continue to be revealed, both experimental and computational epitope mapping are becoming crucial tools in vaccine discovery. Immunoinformatics offers many tools, techniques and approaches for in silico epitope characterization, which is capable of greatly accelerating epitope design.


Assuntos
Epitopos/química , Peptídeos/química , Peptídeos/imunologia , Engenharia de Proteínas/métodos , Imunidade Adaptativa , Biologia Computacional , Desenho de Fármacos , Humanos , Software
11.
BMC Bioinformatics ; 14 Suppl 6: S4, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23735058

RESUMO

BACKGROUND: Allergy is a form of hypersensitivity to normally innocuous substances, such as dust, pollen, foods or drugs. Allergens are small antigens that commonly provoke an IgE antibody response. There are two types of bioinformatics-based allergen prediction. The first approach follows FAO/WHO Codex alimentarius guidelines and searches for sequence similarity. The second approach is based on identifying conserved allergenicity-related linear motifs. Both approaches assume that allergenicity is a linearly coded property. In the present study, we applied ACC pre-processing to sets of known allergens, developing alignment-independent models for allergen recognition based on the main chemical properties of amino acid sequences. RESULTS: A set of 684 food, 1,156 inhalant and 555 toxin allergens was collected from several databases. A set of non-allergens from the same species were selected to mirror the allergen set. The amino acids in the protein sequences were described by three z-descriptors (z1, z2 and z3) and by auto- and cross-covariance (ACC) transformation were converted into uniform vectors. Each protein was presented as a vector of 45 variables. Five machine learning methods for classification were applied in the study to derive models for allergen prediction. The methods were: discriminant analysis by partial least squares (DA-PLS), logistic regression (LR), decision tree (DT), naïve Bayes (NB) and k nearest neighbours (kNN). The best performing model was derived by kNN at k = 3. It was optimized, cross-validated and implemented in a server named AllerTOP, freely accessible at http://www.pharmfac.net/allertop. AllerTOP also predicts the most probable route of exposure. In comparison to other servers for allergen prediction, AllerTOP outperforms them with 94% sensitivity. CONCLUSIONS: AllerTOP is the first alignment-free server for in silico prediction of allergens based on the main physicochemical properties of proteins. Significantly, as well allergenicity AllerTOP is able to predict the route of allergen exposure: food, inhalant or toxin.


Assuntos
Alérgenos/química , Inteligência Artificial , Simulação por Computador , Proteínas/química , Algoritmos , Alérgenos/imunologia , Sequência de Aminoácidos , Teorema de Bayes , Biologia Computacional/métodos , Bases de Dados de Proteínas , Hipersensibilidade Alimentar , Humanos , Hipersensibilidade/imunologia , Proteínas/imunologia , Toxinas Biológicas/química , Toxinas Biológicas/imunologia
12.
Clin Dev Immunol ; 2013: 601943, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24348677

RESUMO

The hepatitis C virus (HCV) is able to persist as a chronic infection, which can lead to cirrhosis and liver cancer. There is evidence that clearance of HCV is linked to strong responses by CD8 cytotoxic T lymphocytes (CTLs), suggesting that eliciting CTL responses against HCV through an epitope-based vaccine could prove an effective means of immunization. However, HCV genomic plasticity as well as the polymorphisms of HLA I molecules restricting CD8 T-cell responses challenges the selection of epitopes for a widely protective vaccine. Here, we devised an approach to overcome these limitations. From available databases, we first collected a set of 245 HCV-specific CD8 T-cell epitopes, all known to be targeted in the course of a natural infection in humans. After a sequence variability analysis, we next identified 17 highly invariant epitopes. Subsequently, we predicted the epitope HLA I binding profiles that determine their potential presentation and recognition. Finally, using the relevant HLA I-genetic frequencies, we identified various epitope subsets encompassing 6 conserved HCV-specific CTL epitopes each predicted to elicit an effective T-cell response in any individual regardless of their HLA I background. We implemented this epitope selection approach for free public use at the EPISOPT web server.


Assuntos
Epitopos de Linfócito T/imunologia , Hepacivirus/imunologia , Hepatite C Crônica/imunologia , Subpopulações de Linfócitos T/imunologia , Sequência de Aminoácidos , Biologia Computacional/métodos , Mapeamento de Epitopos , Epitopos de Linfócito T/química , Epitopos de Linfócito T/metabolismo , Genótipo , Hepacivirus/genética , Antígenos de Histocompatibilidade Classe I/química , Antígenos de Histocompatibilidade Classe I/imunologia , Antígenos de Histocompatibilidade Classe I/metabolismo , Humanos , Modelos Moleculares , Dados de Sequência Molecular , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/imunologia , Ligação Proteica/imunologia , Conformação Proteica , Linfócitos T Citotóxicos/imunologia , Proteínas Virais/química , Proteínas Virais/genética , Navegador
13.
Interdiscip Sci ; 15(1): 131-145, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36550341

RESUMO

Virtual screening (VS) is a computational strategy that uses in silico automated protein docking inter alia to rank potential ligands, or by extension rank protein-ligand pairs, identifying potential drug candidates. Most docking methods use preferred sets of physicochemical descriptors (PCDs) to model the interactions between host and guest molecules. Thus, conventional VS is often data-specific, method-dependent and with demonstrably differing utility in identifying candidate drugs. This study proposes four universality classes of novel consensus scoring (CS) algorithms that combine docking scores, derived from ten docking programs (ADFR, DOCK, Gemdock, Ledock, PLANTS, PSOVina, QuickVina2, Smina, Autodock Vina and VinaXB), using decoys from the DUD-E repository ( http://dude.docking.org/ ) against 29 MRSA-oriented targets to create a general VS formulation that can identify active ligands for any suitable protein target. Our results demonstrate that CS provides improved ligand-protein docking fidelity when compared to individual docking platforms. This approach requires only a small number of docking combinations and can serve as a viable and parsimonious alternative to more computationally expensive docking approaches. Predictions from our CS algorithm are compared against independent machine learning evaluations using the same docking data, complementing the CS outcomes. Our method is a reliable approach for identifying protein targets and high-affinity ligands that can be tested as high-probability candidates for drug repositioning.


Assuntos
Algoritmos , Proteínas , Ligantes , Consenso , Proteínas/química , Simulação de Acoplamento Molecular , Ligação Proteica
14.
Med Biol Eng Comput ; 61(11): 3035-3048, 2023 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-37608081

RESUMO

Extracting "high ranking" or "prime protein targets" (PPTs) as potent MRSA drug candidates from a given set of ligands is a key challenge in efficient molecular docking. This study combines protein-versus-ligand matching molecular docking (MD) data extracted from 10 independent molecular docking (MD) evaluations - ADFR, DOCK, Gemdock, Ledock, Plants, Psovina, Quickvina2, smina, vina, and vinaxb to identify top MRSA drug candidates. Twenty-nine active protein targets (APT) from the enhanced DUD-E repository ( http://DUD-E.decoys.org ) are matched against 1040 ligands using "forward modeling" machine learning for initial "data mining and modeling" (DDM) to extract PPTs and the corresponding high affinity ligands (HALs). K-means clustering (KMC) is then performed on 400 ligands matched against 29 PTs, with each cluster accommodating HALs, and the corresponding PPTs. Performance of KMC is then validated against randomly chosen head, tail, and middle active ligands (ALs). KMC outcomes have been validated against two other clustering methods, namely, Gaussian mixture model (GMM) and density based spatial clustering of applications with noise (DBSCAN). While GMM shows similar results as with KMC, DBSCAN has failed to yield more than one cluster and handle the noise (outliers), thus affirming the choice of KMC or GMM. Databases obtained from ADFR to mine PPTs are then ranked according to the number of the corresponding HAL-PPT combinations (HPC) inside the derived clusters, an approach called "reverse modeling" (RM). From the set of 29 PTs studied, RM predicts high fidelity of 5 PPTs (17%) that bind with 76 out of 400, i.e., 19% ligands leading to a prediction of next-generation MRSA drug candidates: PPT2 (average HPC is 41.1%) is the top choice, followed by PPT14 (average HPC 25.46%), and then PPT15 (average HPC 23.12%). This algorithm can be generically implemented irrespective of pathogenic forms and is particularly effective for sparse data.


Assuntos
Desenho de Fármacos , Proteínas , Simulação de Acoplamento Molecular , Algoritmos , Aprendizado de Máquina
15.
BMC Struct Biol ; 12: 20, 2012 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-22862845

RESUMO

BACKGROUND: HLA-DPs are class II MHC proteins mediating immune responses to many diseases. Peptides bind MHC class II proteins in the acidic environment within endosomes. Acidic pH markedly elevates association rate constants but dissociation rates are almost unchanged in the pH range 5.0 - 7.0. This pH-driven effect can be explained by the protonation/deprotonation states of Histidine, whose imidazole has a pK(a) of 6.0. At pH 5.0, imidazole ring is protonated, making Histidine positively charged and very hydrophilic, while at pH 7.0 imidazole is unprotonated, making Histidine less hydrophilic. We develop here a method to predict peptide binding to the four most frequent HLA-DP proteins: DP1, DP41, DP42 and DP5, using a molecular docking protocol. Dockings to virtual combinatorial peptide libraries were performed at pH 5.0 and pH 7.0. RESULTS: The X-ray structure of the peptide--HLA-DP2 protein complex was used as a starting template to model by homology the structure of the four DP proteins. The resulting models were used to produce virtual combinatorial peptide libraries constructed using the single amino acid substitution (SAAS) principle. Peptides were docked into the DP binding site using AutoDock at pH 5.0 and pH 7.0. The resulting scores were normalized and used to generate Docking Score-based Quantitative Matrices (DS-QMs). The predictive ability of these QMs was tested using an external test set of 484 known DP binders. They were also compared to existing servers for DP binding prediction. The models derived at pH 5.0 predict better than those derived at pH 7.0 and showed significantly improved predictions for three of the four DP proteins, when compared to the existing servers. They are able to recognize 50% of the known binders in the top 5% of predicted peptides. CONCLUSIONS: The higher predictive ability of DS-QMs derived at pH 5.0 may be rationalised by the additional hydrogen bond formed between the backbone carbonyl oxygen belonging to the peptide position before p1 (p-1) and the protonated ε-nitrogen of His79ß. Additionally, protonated His residues are well accepted at most of the peptide binding core positions which is in a good agreement with the overall negatively charged peptide binding site of most MHC proteins.


Assuntos
Antígenos HLA-DP/química , Antígenos HLA-DP/metabolismo , Simulação de Acoplamento Molecular , Peptídeos/química , Peptídeos/metabolismo , Sequência de Aminoácidos , Área Sob a Curva , Sítios de Ligação , Bases de Dados de Proteínas , Histidina/química , Humanos , Ligação de Hidrogênio , Concentração de Íons de Hidrogênio , Dados de Sequência Molecular , Ligação Proteica , Prótons , Reprodutibilidade dos Testes , Alinhamento de Sequência , Termodinâmica
16.
PLoS Pathog ; 6(10): e1001149, 2010 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-20976198

RESUMO

T cell receptor (TCR) recognition of peptide-MHC class I (pMHC) complexes is a crucial event in the adaptive immune response to pathogens. Peptide epitopes often display a strong dominance hierarchy, resulting in focusing of the response on a limited number of the most dominant epitopes. Such T cell responses may be additionally restricted by particular MHC alleles in preference to others. We have studied this poorly understood phenomenon using Theileria parva, a protozoan parasite that causes an often fatal lymphoproliferative disease in cattle. Despite its antigenic complexity, CD8+ T cell responses induced by infection with the parasite show profound immunodominance, as exemplified by the Tp1(214-224) epitope presented by the common and functionally important MHC class I allele N*01301. We present a high-resolution crystal structure of this pMHC complex, demonstrating that the peptide is presented in a distinctive raised conformation. Functional studies using CD8+ T cell clones show that this impacts significantly on TCR recognition. The unconventional structure is generated by a hydrophobic ridge within the MHC peptide binding groove, found in a set of cattle MHC alleles. Extremely rare in all other species, this feature is seen in a small group of mouse MHC class I molecules. The data generated in this analysis contribute to our understanding of the structural basis for T cell-dependent immune responses, providing insight into what determines a highly immunogenic p-MHC complex, and hence can be of value in prediction of antigenic epitopes and vaccine design.


Assuntos
Apresentação de Antígeno/imunologia , Antígenos de Histocompatibilidade Classe I/metabolismo , Epitopos Imunodominantes/metabolismo , Receptores de Antígenos de Linfócitos T/imunologia , Theileria parva/imunologia , Sequência de Aminoácidos , Animais , Sítios de Ligação , Linfócitos T CD8-Positivos/imunologia , Linfócitos T CD8-Positivos/metabolismo , Bovinos , Cristalografia , Antígenos de Histocompatibilidade Classe I/química , Antígenos de Histocompatibilidade Classe I/imunologia , Epitopos Imunodominantes/química , Epitopos Imunodominantes/imunologia , Camundongos , Modelos Moleculares , Ligação Proteica/imunologia , Ligação Proteica/fisiologia , Conformação Proteica , Receptores de Antígenos de Linfócitos T/metabolismo
17.
PLoS Pathog ; 6(2): e1000782, 2010 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-20195504

RESUMO

Tuberculosis (TB) is an escalating global health problem and improved vaccines against TB are urgently needed. HLA-E restricted responses may be of interest for vaccine development since HLA-E displays very limited polymorphism (only 2 coding variants exist), and is not down-regulated by HIV-infection. The peptides from Mycobacterium tuberculosis (Mtb) potentially presented by HLA-E molecules, however, are unknown. Here we describe human T-cell responses to Mtb-derived peptides containing predicted HLA-E binding motifs and binding-affinity for HLA-E. We observed CD8(+) T-cell proliferation to the majority of the 69 peptides tested in Mtb responsive adults as well as in BCG-vaccinated infants. CD8(+) T-cells were cytotoxic against target-cells transfected with HLA-E only in the presence of specific peptide. These T cells were also able to lyse M. bovis BCG infected, but not control monocytes, suggesting recognition of antigens during mycobacterial infection. In addition, peptide induced CD8(+) T-cells also displayed regulatory activity, since they inhibited T-cell proliferation. This regulatory activity was cell contact-dependent, and at least partly dependent on membrane-bound TGF-beta. Our results significantly increase our understanding of the human immune response to Mtb by identification of CD8(+) T-cell responses to novel HLA-E binding peptides of Mtb, which have cytotoxic as well as immunoregulatory activity.


Assuntos
Apresentação de Antígeno/imunologia , Antígenos de Bactérias/imunologia , Linfócitos T CD8-Positivos/imunologia , Antígenos HLA/imunologia , Antígenos de Histocompatibilidade Classe I/imunologia , Mycobacterium tuberculosis/imunologia , Subpopulações de Linfócitos T/imunologia , Adulto , Separação Celular , Epitopos de Linfócito T/imunologia , Citometria de Fluxo , Humanos , Lactente , Ativação Linfocitária/genética , Ativação Linfocitária/imunologia , Linfócitos T Citotóxicos/imunologia , Linfócitos T Reguladores/imunologia , Tuberculose/imunologia , Tuberculose/prevenção & controle , Vacinas contra a Tuberculose/imunologia , Antígenos HLA-E
18.
Nucleic Acids Res ; 38(Database issue): D847-53, 2010 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-19820110

RESUMO

The continuing threat of infectious disease and future pandemics, coupled to the continuous increase of drug-resistant pathogens, makes the discovery of new and better vaccines imperative. For effective vaccine development, antigen discovery and validation is a prerequisite. The compilation of information concerning pathogens, virulence factors and antigenic epitopes has resulted in many useful databases. However, most such immunological databases focus almost exclusively on antigens where epitopes are known and ignore those for which epitope information was unavailable. We have compiled more than 500 antigens into the AntigenDB database, making use of the literature and other immunological resources. These antigens come from 44 important pathogenic species. In AntigenDB, a database entry contains information regarding the sequence, structure, origin, etc. of an antigen with additional information such as B and T-cell epitopes, MHC binding, function, gene-expression and post translational modifications, where available. AntigenDB also provides links to major internal and external databases. We shall update AntigenDB on a rolling basis, regularly adding antigens from other organisms and extra data analysis tools. AntigenDB is available freely at http://www.imtech.res.in/raghava/antigendb and its mirror site http://www.bic.uams.edu/raghava/antigendb.


Assuntos
Antígenos/química , Biologia Computacional/métodos , Bases de Dados Genéticas , Sistema Imunitário/metabolismo , Doenças Transmissíveis/imunologia , Doenças Transmissíveis/metabolismo , Biologia Computacional/tendências , Bases de Dados de Proteínas , Epitopos/química , Humanos , Imunogenética/métodos , Armazenamento e Recuperação da Informação/métodos , Internet , Linfócitos/imunologia , Linfócitos/metabolismo , Mapeamento de Peptídeos , Processamento de Proteína Pós-Traducional , Estrutura Terciária de Proteína , Software
19.
BMC Struct Biol ; 11: 32, 2011 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-21752305

RESUMO

BACKGROUND: MHC class II proteins bind oligopeptide fragments derived from proteolysis of pathogen antigens, presenting them at the cell surface for recognition by CD4+ T cells. Human MHC class II alleles are grouped into three loci: HLA-DP, HLA-DQ and HLA-DR. In contrast to HLA-DR and HLA-DQ, HLA-DP proteins have not been studied extensively, as they have been viewed as less important in immune responses than DRs and DQs. However, it is now known that HLA-DP alleles are associated with many autoimmune diseases. Quite recently, the X-ray structure of the HLA-DP2 molecule (DPA*0103, DPB1*0201) in complex with a self-peptide derived from the HLA-DR α-chain has been determined. In the present study, we applied a validated molecular docking protocol to a library of 247 modelled peptide-DP2 complexes, seeking to assess the contribution made by each of the 20 naturally occurred amino acids at each of the nine binding core peptide positions and the four flanking residues (two on both sides). RESULTS: The free binding energies (FBEs) derived from the docking experiments were normalized on a position-dependent (npp) and on an overall basis (nap), and two docking score-based quantitative matrices (DS-QMs) were derived: QMnpp and QMnap. They reveal the amino acid preferences at each of the 13 positions considered in the study. Apart from the leading role of anchor positions p1 and p6, the binding to HLA-DP2 depends on the preferences at p2. No effect of the flanking residues was found on the peptide binding predictions to DP2, although all four of them show strong preferences for particular amino acids. The predictive ability of the DS-QMs was tested using a set of 457 known binders to HLA-DP2, originating from 24 proteins. The sensitivities of the predictions at five different thresholds (5%, 10%, 15%, 20% and 25%) were calculated and compared to the predictions made by the NetMHCII and IEDB servers. Analysis of the DS-QMs indicated an improvement in performance. Additionally, DS-QMs identified the binding cores of several known DP2 binders. CONCLUSIONS: The molecular docking protocol, as applied to a combinatorial library of peptides, models the peptide-HLA-DP2 protein interaction effectively, generating reliable predictions in a quantitative assessment. The method is structure-based and does not require extensive experimental sequence-based data. Thus, it is universal and can be applied to model any peptide - protein interaction.


Assuntos
Alelos , Antígenos HLA-DP/química , Peptídeos/química , Sítios de Ligação , Cristalografia por Raios X , Genes MHC da Classe II , Antígenos HLA-DP/genética , Cadeias beta de HLA-DP , Humanos , Modelos Moleculares , Termodinâmica
20.
Bioinformatics ; 26(16): 2066-8, 2010 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-20576624

RESUMO

MOTIVATION: T-cell epitope identification is a critical immunoinformatic problem within vaccine design. To be an epitope, a peptide must bind an MHC protein. RESULTS: Here, we present EpiTOP, the first server predicting MHC class II binding based on proteochemometrics, a QSAR approach for ligands binding to several related proteins. EpiTOP uses a quantitative matrix to predict binding to 12 HLA-DRB1 alleles. It identifies 89% of known epitopes within the top 20% of predicted binders, reducing laboratory labour, materials and time by 80%. EpiTOP is easy to use, gives comprehensive quantitative predictions and will be expanded and updated with new quantitative matrices over time. AVAILABILITY: EpiTOP is freely accessible at http://www.pharmfac.net/EpiTOP.


Assuntos
Epitopos de Linfócito T/metabolismo , Antígenos de Histocompatibilidade Classe II/metabolismo , Software , Alelos , Antígenos HLA-DR/metabolismo , Cadeias HLA-DRB1 , Antígenos de Histocompatibilidade Classe II/genética , Ligantes , Peptídeos/imunologia , Peptídeos/metabolismo
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