The scaffold protein, Homer1b/c, regulates axon pathfinding in the central nervous system in vivo.

Homer proteins are a family of multidomain cytosolic proteins that have been postulated to serve as scaffold proteins that affect responses to extracellular signals by regulating protein-protein interactions. We tested whether Homer proteins are involved in axon pathfinding in vivo, by expressing both wild-type and mutant isoforms of Homer in Xenopus optic tectal neurons. Time-lapse imaging demonstrated that interfering with the ability of endogenous Homer to form protein-protein interactions resulted in axon pathfinding errors at stereotypical choice points. These data demonstrate a function for scaffold proteins such as Homer in axon guidance. Homer may facilitate signal transduction from cell-surface receptors to intracellular proteins that govern the establishment of axon trajectories.

The ontogeny of GABA- and glutamate-like immunoreactivity in the embryonic Australian freshwater crayfish, Cherax destructor.

The distribution and ontogeny of GABA- and glutamate-like immunoreactivity in embryos of the Australian freshwater crayfish Cherax destructor were investigated over the period from 30% development until hatching. GABA-like immunoreactive cells and fibres appeared first in the brain at 40-45% development. By 70% development, GABA-like immunoreactive cells were present in almost all ganglia, and GABA-like immunoreactive fibres were distributed extensively throughout the neuropil, commissures and connectives of the central nervous system, and were also found in peripheral nerve roots supplying the appendages and the abdominal musculature. In contrast, glutamate-like immunoreactivity did not appear in the central nervous system until 60-65% development. By the time of hatching, the distribution of glutamate-like immunoreactivity was restricted to discrete regions of neuropil and fibre staining in the thoracic and abdominal nerve cord, the abdominal musculature and the appendages. The precocious establishment of the extensive distribution of GABA-like immunoreactive neurons in the developing crayfish embryo is consistent with the possibility that these neurons play a trophic role in controlling or modulating the development of the nervous system.