The role of 20E biosynthesis relative gene Shadow in the reproduction of the predatory mirid bug, Cyrtorhinus lividipennis (Hemiptera: Miridae).

Cytorhinus lividipennis is a natural enemy of rice planthoppers and leafhoppers. Improving the fecundity of C. lividipennis will be helpful to improve its control effect on pests. However, little is known about the hormonal regulatory mechanism of reproduction in C. lividipennis. In the current study, we examined the role of 20-hydroxyecdysone (20E) biosynthesis relative gene Shadow in the reproduction of C. lividipennis. The complementary DNA sequence of ClSad is 2018 -bp in length with an open reading frame of 1398-bp encoding 465 amino acid residues. ClSad was readily detected in nymphal and adult stages, and highly expressed in the adult stage. ClSad was highly expressed in the midgut and ovaries of adult females. Moreover, RNA interference-mediated knockdown of ClSad reduced the 20E titers and ClVg transcript level, resulting in fewer fully developed eggs and a decrease in the number of eggs laid by dsSad-injected adult females within 15 days. These results suggest that ClSad plays a critical role in the reproduction of C. lividipennis. The present study provides insights into the molecular mechanism of the ClSad gene for the reproduction of C. lividipennis.

Mating behavior and responses to sublethal concentrations of imidacloprid in the predator Cyrtorhinus lividipennis.

BACKGROUND: Mating is an essential factor that governs the size of insect populations that reproduce sexually. The extensive application of insecticides has both lethal and sublethal effects on the physiology and mating behavior of insect natural enemies. The predatory bug Cyrtorhinus lividipennis is a natural enemy of planthopper and leafhopper populations in the rice ecosystem. Unfortunately, the effects of insecticides on the mating behavior of C. lividipennis are not well-understood. RESULTS: The mating behavior of C. livdipennis consisted of mounting, antennal touch and mating attempts, genital insertion, adjustment of posture, and separation of the mating pair. Approximately 82.5% of the C. lividipennis mating pairs displayed their first mating at 12-36 h postemergence. Mating activity occurred throughout a 24-h period, with peak activity at 12:00-14:00 h, and the mean duration of mating was 48.75 min. Sublethal exposure to imidacloprid increased mating latency. Compared with the controls, the duration of courtship, pre-mating and adjusting posture for males treated with imidacloprid were prolonged. The duration of mating for females treated with imidacloprid was prolonged relative to untreated controls. The fecundity and daily spawning capacity of females treated with imidacloprid were higher than the untreated controls. CONCLUSION: Our results provide insight into the mating process of C. lividipennis. Imidacloprid prolonged the duration of mating, which may explain the enhanced reproductive output in C. lividipennis females treated with imidacloprid. These findings will be useful in both rearing C. lividipennis and deploying this natural enemy in rice fields. © 2024 Society of Chemical Industry.

Diagnostic value of circulating miR-155 for breast cancer: a meta-analysis.

Backgrounds: The value of circulating microRNA (miR)-155 for breast cancer (BC) diagnosis may differ in different studies. Therefore, we conducted this systematic review and meta-analysis to evaluate the potential application of circulating miR-155 in the diagnosis of BC. Methods: Articles published before December 2023 and in English were searched in these databases: PubMed, Web of Science, Medline, EMBASE and Google Scholar. A summary of sensitivity, specificity, positive likelihood ratios (PLR), negative likelihood ratios (NLR), and diagnostic odds ratio (DOR) were calculated from the true positive (TP), true negative (TN), false positive (FP) and false negative (FN) of each study. Additionally, the summary receive-operating characteristics (SROC) curve was constructed to summarize the TP and FP rates. Results: The pooled parameters calculated were as follows: sensitivity, 0.93 (95% CI: 0.83-0.97); specificity, 0.85 (95% CI: 0.74-0.92); PLR, 6.4 (95% CI: 3.4-11.9); NLR, 0.09 (95% CI: 0.04-0.20); and DOR, 74 (95% CI: 22-247). The analysis showed a significant heterogeneity (sensitivity, I2 = 95.19%, p < 0.001; specificity, I2 = 95.29%, p < 0.001; DOR, I2 = 92.9%, p < 0.001). The SROC curve was with an area under curve (AUC) of 0.95 (95% CI: 0.93-0.97). Conclusion: Circulating miR-155 has a potential in the diagnosis of BC.

The prognostic effect of metastasis patterns on overall survival in organ metastatic lung adenocarcinoma.

The effect of various metastases patterns on the prognosis of lung adenocarcinoma (AD) remains unknown. The purpose of our retrospective study is to determine whether various metastases patterns have a prognostic impact on patients with organ metastatic lung AD. Patient data was extracted from the Surveillance, Epidemiology, and End Results (SEER) database. The Kaplan-Meier method was chosen for the evaluation of overall survival (OS) rate. Univariable and multivariable Cox regression analyses were conducted to evaluate independent prognostic factors. In the SEER database, 12,228 patients with IV lung AD were retrieved in total. And 78.78% of those patients (9633/12,228) suffered from one of brain, lung, liver or bone metastasis due to disease progression. It was found that the most common site in patients with metastatic lung AD was brain (21.20%), and the least common site of metastasis was liver (3.50%). Patients who suffered single lung metastatic showed relatively good OS, and the median survival time was 11 months (95% CI 0.470-0.516). For those with 2 metastatic sites, data analysis suggested that the median survival times of patients with bone and lung metastasis (10 months; 95% CI 0.469-0.542) were better than others. For those with 3 metastatic sites, data analysis suggested that metastatic pattern had no effect on the OS. Brain is the most common single metastasis site of lung AD. Compared with the other 3 metastatic sites, patients with lung metastasis had better survival results. Deeper knowledge of metastatic patterns will help doctors to better understand the prognosis and formulate more appropriate treatment plans.