[Effect of elemene on reversing chemoresistance to adriamycin in human stomach cancer cell line].

OBJECTIVE: To investigate the effect of elemene on reversing chemoresistance to adriamycin (ADM) in human stomach cancer cell, and explore its possible mechanism. METHODS: SGC7901/ADM were divided imto two groups: control group and elemene treatment group. The cytotoxicity of ADM on SGC7901/ADM was determined by MTT assay. The activity of nuclear factor-kappa B (NF-kappaB) was measured by immunohistochemical staining. The apoptosis rate of stomach cancer cell line was determined by flow cyotometric analysis. RESULTS: The immunohistochemical staining result showed that the activity of NF-kappaB in SGC7901/ADM was increased after treated with ADM for 9 - 12 h, while that of the elemene treatment group decreased with the increasing of the elemene concentration and the lowest level was 8 - 12%. The apoptosis rate of SGC7901/ADM stomach cancer cell line was increased with the increasing of elemene concentration. At the same elemene concentration, the apoptosis rate increased with ADM treatment time prolonged. MTT result showed that the non-cytotoxic dose of elemene had synergistic effect on rilling SGC7901/ADM stomach cancer cell line and was in a dose-dependant manner. CONCLUSION: The inhibitory effect of elemene on reversing chemoresistance to ADM in human stomach cancer cell line maybe related to inhibiting NF-kappaB activity.

Effects of Coriolus versicolor polysaccharide B on monocyte chemoattractant protein 1 gene expression in rat.

AIM: To investigate the effect of Coriolus versicolor polysaccharide B (CVPS-B), a new water-soluble component of polysaccharides from the fungus Coriolus versicolor (Fr) L on monocyte chemoattractant protein-1 (MCP-1) gene expression in rat splenocytes. METHODS: Expression of MCP-1 mRNA in rat splenocytes was examined by reverse transcription-polymerase chain reaction (RT-PCR) with beta- actin as an internal standard. Sequencing of RT-PCR products was performed to confirm their specificity in MCP-1 gene composition. RESULTS: (1) Without pre-treatment of lipopolysaccharide (LPS), the relative MCP-1 mRNA expression ratios (MCP-1/beta-actin) for the saline control group and for CVPS-B groups in 3 different doses (10, 20, and 30 mg . kg-1 . d-1, ip, for 4 d) were 1.4 +/- 0.3, 1.6 +/- 0.4, 1.7 +/- 0.5, and 1.5 +/- 0.4, respectively (P > 0.05); (2) LPS (10 microg . kg-1, ip) enhanced the expression of MPC-1 mRNA by the ratio of 114 %; (3) pre-treatment with CVPS-B of 4 different doses (5, 10, 30, and 50 mg . kg-1 . d-1, ip, for 4 d) decreased the LPS induced expression of MPC-1 mRNA by the ratios of 51 %, 70 %, 84 %, and 99 %, respectively (n = 6). CONCLUSION: In a dose-related fashion, CVPS-B inhibited the expression of MCP-1 mRNA induced by LPS in the rat splenocytes, but did not significantly affect the expression of MPC-1 mRNA in the normal rat.