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1.
J. appl. oral sci ; 25(1): 42-52, Jan.-Feb. 2017. graf
Artigo em Inglês | LILACS, BBO | ID: biblio-841161

RESUMO

Abstract Sodium alendronate is a bisphosphonate drug that exerts antiresorptive action and is used to treat osteoporosis. Objective The aim of this study was to evaluate the bone repair process at the bone/implant interface of osteoporotic rats treated with sodium alendronate through the analysis of microtomography, real time polymerase chain reactions and immunohistochemistry (RUNX2 protein, bone sialoprotein (BSP), alkaline phosphatase, osteopontin and osteocalcin). Material and Methods A total of 42 rats were used and divided in to the following experimental groups: CTL: control group (rats submitted to fictitious surgery and fed with a balanced diet), OST: osteoporosis group (rats submitted to a bilateral ovariectomy and fed with a low calcium diet) and ALE: alendronate group (rats submitted to a bilateral ovariectomy, fed with a low calcium diet and treated with sodium alendronate). A surface treated implant was installed in both tibial metaphyses of each rat. Euthanasia of the animals was conducted at 14 (immunhostochemistry) and 42 days (immunohistochemistry, micro CT and PCR). Data were subjected to statistical analysis with a 5% significance level. Results Bone volume (BV) and total pore volume were higher for ALE group (P<0.05). Molecular data for RUNX2 and BSP proteins were significantly expressed in the ALE group (P<0.05), in comparison with the other groups. ALP expression was higher in the CTL group (P<0.05). The immunostaining for RUNX2 and osteopontin was positive in the osteoblastic lineage cells of neoformed bone for the CTL and ALE groups in both periods (14 and 42 days). Alkaline phosphatase presented a lower staining area in the OST group compared to the CTL in both periods and the ALE at 42 days. Conclusion There was a decrease of osteocalcin precipitation at 42 days for the ALE and OST groups. Therefore, treatment with short-term sodium alendronate improved bone repair around the implants installed in the tibia of osteoporotic rats.


Assuntos
Animais , Feminino , Osteoporose/tratamento farmacológico , Implantes Dentários , Osseointegração/efeitos dos fármacos , Alendronato/farmacologia , Conservadores da Densidade Óssea/farmacologia , Osteoblastos/efeitos dos fármacos , Osteoporose/fisiopatologia , Tíbia/cirurgia , Fatores de Tempo , Imuno-Histoquímica , Ovariectomia , Densidade Óssea/efeitos dos fármacos , Osteocalcina/análise , Osteocalcina/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Reprodutibilidade dos Testes , Ratos Wistar , Implantes Experimentais , Implantação Dentária Endóssea , Fosfatase Alcalina/análise , Fosfatase Alcalina/efeitos dos fármacos , Subunidade alfa 1 de Fator de Ligação ao Core/análise , Subunidade alfa 1 de Fator de Ligação ao Core/efeitos dos fármacos , Osteopontina/análise , Osteopontina/efeitos dos fármacos , Microtomografia por Raio-X , Reação em Cadeia da Polimerase em Tempo Real
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