[Effects of ApoE gene polymorphism on the efficacy of atorvastatin in the treatment of hyperlipidemia].

Objective: To study the role of ApoE gene polymorphism on efficacy of atorvastatin in lowering the lipid and its clinical significance. Methods: A total of 962 patients with hypercholesterolemia were selected between January 1 st and December 31 st 2014. The ApoE genepolymorphism in patients with hyperlipidemia was performed by using polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP) method in translational medicine center of Huaihe Hospital. Patients with ApoE genotype E3/3 and E3/4 were selected and treated with atorvastatin 10 mg/d for 4 weeks. Before and after treatment, triglycerides (TG) and total cholesterol (TC) was detected by enzyme colorimetry method. High-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were performed by Clearance method. Lipoprotein(a) (Lp(a)) was performed by turbidimetric inhibition immunoassay. ApoE gene expression was performed by real-time PCR. Results: In the 6 gene types, the frequencies of E3/4 and E3/3 were 30.6% (294 cases) and 59.1% (569 cases) respectively. After treatment with atorvastatin, the change percent of TC, LDL-C, HDL-C, TG, Lp(a) in E3/4 and E3/3 group were -(23.0±4.7)% vs -(12.0±3.1)% (P<0.001), -(33.0±4.8)% vs -(20.0±3.9)% (P<0.001), (18.0±3.8)% vs (6.0±2.6)% (P<0.001), -(23.0±3.9)% vs -(13.0±2.7)% (P<0.001), -(21.5±4.5)% vs -(20.9±4.0)% (P=0.054), respectively. ApoE gene expression in E3/3 and E3/4 groups were down-regulated in both groups, and the change in E3/3 group was obvious than that of E3/4 group. Conclusion: After treatment with atorvastatin, levels of lipids and ApoE gene expression in ApoE genotype E3/3 patients decreased, which were more evident than E3/4 patients.

The role of glutamine supplemented total parenteral nutrition (TPN) in severe acute pancreatitis.

OBJECTIVE: To evaluate the role of glutamine-supplemented total parenteral nutrition (TPN) in severe acute pancreatitis. PATIENTS AND METHODS: Forty-seven patients with severe acute pancreatitis were admitted to Huaihe Hospital, China, over a period of one year (July 2013 to June 2014) were randomly divided into two therapeutic groups. Patients in group 1 (24 patients in total) and group 2 (23 patient in total) were treated with glutamine-supplemented TPN and standard TPN respectively. Patients were assessed for nutritional parameters, the prevalence of complications, mortality, length of hospital stay (LOS) and length of TPN. RESULTS: The majority of patients were male in both groups (62.5% in group 1 and 60.9% in group 2) and the average age was similar (39.13±4.46 years in group 1 and 40±3.96 years in group 2). The major causative factor was also similar in both groups, i.e. gall stones. The prevalence of complications in the group 2 was much higher (47.85%) than those in the group 1 (25%). The mortality rate for group 1 and 2 were 4.2% (1/24) and 17.4% (4/23), respectively. The length of hospital stay in the group 2 (23.08 ± 2.02 days) was longer than those of the group 1 (20.33 ± 2.40 days). The length of TPN was also longer in the group 2 (16.47 ± 2.72 days) than those of the group 1 (10.56 ± 2.21 days). Glutamine was also associated with significant increase in serum albumin level. CONCLUSIONS: Glutamine-supplemented TPN can reduce the mortality and the occurrence of complications, shorten the length of stay and improve the nutritional status of the patients with severe acute pancreatitis.