Ameliorated 3 × 3 coupler-based demodulation algorithm using iteratively reweighted ellipse specific fitting.

Passive demodulation scheme using 3 × 3 coupler has been widely used in phase-sensitive optical time-domain reflectometry (φ-OTDR), interrogation of fiber Bragg gratings or fiber optic interferometric sensors, and sensor multiplexing. However, the asymmetry of the 3 × 3 coupler in real applications affects the demodulation performance seriously. We proposed an ameliorated 3 × 3 coupler-based demodulation algorithm using iteratively reweighted ellipse specific fitting (IRESF) to overcome the drawback. IRESF combines iterative reweight technology with ellipse specific fitting, which decreases the weights of high noise points and always outputs ellipse solutions. Any two output signals from the 3 × 3 coupler-based interferometer are fitted by the IRESF and then corrected as a pair of quadrature signals. The stability of the fitting parameters is utilized to resolve the failures of IRESF under small signals. A real-time 1/4 ellipse arc judging module is designed, if the Lissajous figure is larger than 1/4 ellipse arc, IRESF is executed to offer ellipse correction parameters. Otherwise, the fixed parameters preset in the algorithm are used. The fixed parameters are mean values of the fitting parameters of IRESF under a large stimulus. The desired phase signal is finally extracted from the corrected quadrature signals. Experimental results show that the ameliorated algorithm does not require strict symmetry of the 3 × 3 coupler and can work under small signals. The noise floor of the proposed algorithm is -112 dB re rad/√Hz and the demodulated amplitude is 23.15 dB (14.37 rad) at 1 kHz when THD is 0.0488%. Moreover, the response linearity is as high as 99.999%. Compared to the algorithm using direct least squares, the proposed demodulation algorithm is more robust and precise, which has broad application prospects.

Nonlinear error elimination using the fusion of PGC-DCM, geometric fitting, and Atan algorithms.

A phase generated carrier (PGC) demodulation scheme is always accompanied by nonlinear errors. We propose a fusion of PGC differential and cross multiplying (PGC-DCM), geometric fitting, and arctangent (Atan) algorithms for fiber optic interferometric sensors to eliminate nonlinear errors. The output amplitude of the PGC-DCM algorithm is used to judge whether the Lissajous figure of quadrature signals is larger than 1/2 ellipse arc. When the Lissajous figure exceeds 1/2 ellipse arc, the contaminated quadrature signals are corrected by the ellipse correction parameters calculated from the geometric fitting; otherwise, the previous fitting parameters are employed for correction. Geometric fitting is realized by minimizing the Sampson error, and its failure problem under small signals is solved by using the temporary stability of fitting results. Finally, desired signals are extracted from the corrected quadrature signals by the Atan algorithm. Experimental results show that the fusion combines the merits of the three algorithms and expands the application of the geometric fitting in PGC demodulation schemes.

High precision 3 × 3 coupler demodulation algorithm with an additional phase shift judgment module.

Ellipse fitting algorithms (EFAs) have been widely used in 3×3 coupler demodulation systems to reduce the requirement for symmetry of the 3×3 couplers. Based on the relative stability of the splitting ratio and phase difference after the establishment of the 3×3 coupler demodulation system, we solve the problem that EFA fails to work when the stimulating signal is small. Depending on the existence of a symmetry point about the origin, an additional phase shift judgment module is used to determine whether the Lissajous figure is larger than π rad. If the elliptical arc exceeds π rad, the EFA is executed. Otherwise, the previous parameters are used to correct the ellipse. Parameters are updated in real time to ensure high precision. The experimental results show that the total harmonic distortion (THD) of the ameliorated algorithm is improved by 1.28% compared to the EFA without the judgment module with a stimulus amplitude of 30 mV. The proposed scheme can effectively improve the dynamic range of the 3×3 coupler demodulation to reach 125.64 dB @ 1 kHz and 1% THD. The algorithm ensures the effective operation of the EFA under small phase shift conditions and improves the accuracy of phase demodulation.

Fiber optic temperature and strain sensor using dual Mach-Zehnder interferometers.

A fiber optic temperature and strain sensor using dual Mach-Zehnder interferometers (MZIs) is proposed. The dual MZIs were fabricated by fusion splicing of two different fibers between two single-mode fibers. The two fibers of thin-core fiber and small-cladding polarization maintaining fiber were fusion spliced with a core offset. As the responses of the two MZIs are different in terms of temperature and strain, simultaneous temperature and strain measurement were experimentally validated by selecting two resonant dips in the transmission spectrum to construct a matrix. Experimental results show that the proposed sensors had the maximum temperature sensitivity of 66.67 pm/°C and the maximum strain sensitivity of -2.0p m/µÎµ. The minimum discriminated temperature and strain of the two proposed sensors were 0.20°C and 0.71 µÎµ, and 0.33°C and 0.69 µÎµ, respectively. The proposed sensor has promising application prospects due to the merits of ease of fabrication, low costs, and good resolution.

Genome-Wide Identification and Analysis of FKBP Gene Family in Wheat (Triticum asetivum).

FK506-binding protein (FKBP) genes have been found to play vital roles in plant development and abiotic stress responses. However, limited information is available about this gene family in wheat (Triticum aestivum L.). In this study, a total of 64 FKBP genes were identified in wheat via a genome-wide analysis involving a homologous search of the latest wheat genome data, which was unevenly distributed in 21 chromosomes, encoded 152 to 649 amino acids with molecular weights ranging from 16 kDa to 72 kDa, and was localized in the chloroplast, cytoplasm, nucleus, mitochondria, peroxisome and endoplasmic reticulum. Based on sequence alignment and phylogenetic analysis, 64 TaFKBPs were divided into four different groups or subfamilies, providing evidence of an evolutionary relationship with Aegilops tauschii, Brachypodium distachyon, Triticum dicoccoides, Arabidopsis thaliana and Oryza sativa. Hormone-related, abiotic stress-related and development-related cis-elements were preferentially presented in promoters of TaFKBPs. The expression levels of TaFKBP genes were investigated using transcriptome data from the WheatExp database, which exhibited tissue-specific expression patterns. Moreover, TaFKBPs responded to drought and heat stress, and nine of them were randomly selected for validation by qRT-PCR. Yeast cells expressing TaFKBP19-2B-2 or TaFKBP18-6B showed increased influence on drought stress, indicating their negative roles in drought tolerance. Collectively, our results provide valuable information about the FKBP gene family in wheat and contribute to further characterization of FKBPs during plant development and abiotic stress responses, especially in drought stress.

Discovery of Simple Diacylhydrazine-Functionalized Cinnamic Acid Derivatives as Potential Microtubule Stabilizers.

To develop novel microtubule-binding agents for cancer therapy, an array of N-cinnamoyl-N'-(substituted)acryloyl hydrazide derivatives were facilely synthesized through a two-step process. Initially, the antiproliferative activity of these title compounds was explored against A549, 98 PC-3 and HepG2 cancer cell lines. Notably, compound I23 exhibited the best antiproliferative activity against three cancer lines with IC50 values ranging from 3.36 to 5.99 µM and concurrently afforded a lower cytotoxicity towards the NRK-52E cells. Anticancer mechanism investigations suggested that the highly bioactive compound I23 could potentially promote the protofilament assembly of tubulin, thus eventually leading to the stagnation of the G2/M phase cell cycle of HepG2 cells. Moreover, compound I23 also disrupted cancer cell migration and significantly induced HepG2 cells apoptosis in a dosage-dependent manner. Additionally, the in silico analysis indicated that compound I23 exhibited an acceptable pharmacokinetic profile. Overall, these easily prepared N-cinnamoyl-N'-(substituted)acryloyl hydrazide derivatives could serve as potential microtubule-interacting agents, probably as novel microtubule-stabilizers.

Phase-shifted demodulation technique with additional modulation based on a 3 × 3 coupler and EFA for the interrogation of fiber-optic interferometric sensors.

A phase-shifted demodulation technique with a 3×3 coupler and ellipse fitting algorithm (EFA) for the interrogation of interferometric sensors is proposed. To reduce the error of the EFA as to measure small phase signals, additional phase modulation is introduced. The additional modulation provides a walk of the operating point along the Lissajous ellipse large enough to permit calculation of the ellipse parameters at every moment. Experimental result shows that this technique demonstrates high accuracy and stability for measuring small phase signals. The setting of this technology expands the application of the EFA in fiber-optic phase demodulation technology.

Large-range phase-difference sensing technology for low-frequency strain interrogation.

Phase-difference sensing technology (PDST) has been applied to strain measurement, but its completeness is destroyed by the phase-difference measurement range. A scheme that can realize the completeness of the PDST for low-frequency strain interrogation is proposed. It is built on dual-interferometers and the elliptic-fitting algorithm. To break the measurement range limitation (0, π), a phase compensation setting is applied. The experimental results demonstrate that the method can obtain low-frequency strain signals, and the low-frequency signal whose phase amplitude is greater than π is recovered. The scheme is an efficient and complete method for measuring the strain of low-frequency optical fiber length, which could be applied to low-frequency seismic wave monitoring and rock deformation detection.

Extensive antimicrobial resistance and plasmid-carrying resistance genes in mcr-1-positive E. coli sampled in swine, in Guangxi, South China.

BACKGROUND: The discovery of the superbug mcr-1-positive Escherichia coli (MCRPEC) has drew greet attention. Swine-origin multi-drug resistant MCRPEC has been a potential threat to public health and safety. However, there were few detailed studies have been reported on swine MCRPEC in Guangxi, South China. RESULTS: In this study, thirty-three MCRPEC strains were detected from 142 E. coli strains from 116 samples in Guangxi in 2018. Which could be classified into eight unique STs and a total of six incompatibility plasmid groups (IncFI, IncHI1, IncY, IncN, IncI1 and IncX1). After that, the susceptibility of MCRPEC isolates to 27 antimicrobial agents belonging to 17 antimicrobial categories was tested. There were nineteen E. coli resistant to 3rd and 4th generation cephalosporins and twelve E. coli resistant to carbapenem resistan. Importantly, the MCRPEC showed high resistance highly resistance for imipenem and meropenem, which were forbidden to use in livestock production. Three MCRPEC strains were further proved to be extensively drug-resistant (XDR), and the other isolates were multi-drug-resistant (MDR). Furthermore, we found that the plasmid-carrying resistance genes coexisted with the mcr-1 gene of the MCRPEC isolates. Which were listed as follows: ß-lactamase antimicrobial resistance genes e.g. ESBL genes (blaCTX-M14, blaCTX-M24, blaCTX-M123, blaOXA-1), plasmid-mediated AmpC (pAmpC) gene (blaCMY-2), the carbapenem resistance gene (blaNDM-5), and non-ß-lactamase antimicrobial resistance genes (qnrA, qnrB, qnrS, aac (6')-Ib-cr, tetA, tetB, sul1, sul2, floR, aadA). CONCLUSION: Thirty-three mcr-1-positive E. coli isolates in Guangxi displayed a wide profile of antimicrobial resistance. Plasmid-carrying resistance genes might be the main cause of MCRPEC multidrug resistance. This study highlighted the necessity for long-term surveillance of mcr-1-positive E. coli in pigs.

Cyclophilin OsCYP20-2 with a novel variant integrates defense and cell elongation for chilling response in rice.

Coordinating stress defense and plant growth is a survival strategy for adaptation to different environments that contains a series of processes, such as, cell growth, division and differentiation. However, little is known about the coordination mechanism for protein conformation change. A cyclophilin OsCYP20-2 with a variant interacts with SLENDER RICE1 (SLR1) and OsFSD2 in the nucleus and chloroplasts, respectively, to integrate chilling tolerance and cell elongation in rice (Oryza sativa) (FSD2, Fe-superoxide dismutase 2). Mass spectrum assay showed that OsNuCYP20-2 localized at the nucleus (nuclear located OsCYP20-2) was a new variant of OsCYP20-2 that truncated 71 amino-acid residues in N-terminal. The loss-of function OsCYP20-2 mutant showed sensitivity to chilling stress with accumulation of extra reactive oxygen species (ROS). In chloroplasts, the full-length OsCYP20-2 promotes OsFSD2 forming homodimers which enhance its activity, eliminating the accumulation of ROS under chilling stress. However, the mutant had shorter epidermal cells in comparison with wild-type Hwayoung (HY). In the nucleus, OsCYP20-2 caused conformation change of SLR1 to promote its degradation for cell elongation. Our data reveal a cyclophilin with a variant with dual-localization in chloroplasts and the nucleus, which mediate chilling tolerance and cell elongation.

Axial strain applied in-fiber Mach-Zehnder interferometer for acceleration measurement.

We present an axial strain applied in-fiber Mach-Zehnder interferometer (MZI) for acceleration measurement. A thin core fiber is sandwiched between two single-mode fibers with core offset to form the MZI. A controlled high fringe visibility in the transmission spectrum is obtained by applying an axial strain, leading to a large slope at the quadrature point. The MZI is then clamped to work as an accelerometer. Experimental results show that the resolution achieves 86 ng/√Hz (g is gravity of 9.8 m/s2), the dynamic range reaches as large as 104.1 dB and the linearity of acceleration response is as high as 99.994%. Moreover, the resonance frequency can be tailored by the clamped fiber length and applied axial strain. The proposed sensor is attractive for practical applications due to low temperature crosstalk, compact size and high sensitivity.

Real-time acceleration sensing with an arctan algorithm based on a modal interferometer.

This study proposes a fiber-optic accelerometer for low-frequency vibration signal detection. The phase velocities of the polarization eigenmodes are affected differently by signals, leading to a polarization rotation of the transmitted lights. The orthogonal square roots of the photovoltages are utilized for an arctan demodulation scheme. Experimental results show that it provides a flat response of 75.04 mrad/g, an average resolution of 13.44 µg/√Hz, and a dynamic range of 111.62 dB below 180 Hz. The environmental instability and sensor complexity are significantly reduced, so that the sensor can be further used in the warning of coal and gas outburst.

[Haze Spectral Analysis and Detection Algorithm Using Satellite Remote Sensing Data].

Frequent occurring of haze pollution events and high fine particulate matter (PM(2.5)) concentration in China have attracted more and more attention in the world. Satellite remote sensing can be used to characterize the air pollution. However, haze is usually misidentified as fog, thin cloud or bright surface in NASA's Moderate Resolution Imaging Spectrometer (MODIS) cloud and clear days' aerosol products, and the retrieval of its optical properties is not included in MODIS cloud detection and dark target algorithm. This approach first studies the spectral characters of cloud, fog, haze, and land cover pixels. Second, following the previous cloud detection and aerosol retrieval literatures, a threshold algorithm is developed to distinguish haze from other pixels based on MODIS multi-band apparent reflectance and brightness temperature. This algorithm is used to detect the haze distribution over North China Plain in 2008 spring and summer. Our result shows a good agreement with the true-color satellite images, which enhances MODIS's ability to monitor the severe air pollution episodes. In addition, the high AOD data from Beijing and Xiang Aerosol Robotic NETwork (AERONET) sites indicate nearly 80% haze days are detected by our approach. Finally, we analyze the errors and uncertainties in haze detection algorithm, and put forward the potential improvements.

Metabolite regulation of nucleo-cytosolic trafficking of carbohydrate response element-binding protein (ChREBP): role of ketone bodies.

The carbohydrate response element-binding protein (ChREBP) is a glucose-responsive transcription factor that plays a critical role in converting excess carbohydrate to storage fat in liver. In response to changing glucose levels, ChREBP activity is regulated by nucleo-cytoplasmic shuttling of ChREBP via interactions with 14-3-3 proteins and importins. The nuclear/cytosol trafficking is regulated partly by phosphorylation/dephosphorylation of serine 196 mediated by cAMP-dependent protein kinase and protein phosphatase. We show here that protein-free extracts of starved and high fat-fed livers contain metabolites that activate interaction of ChREBP·14-3-3 and inhibit the ChREBP/importin α interaction, resulting in cytosolic localization. These metabolites were identified as ß-hydroxybutyrate and acetoacetate. Nuclear localization of GFP-ChREBP is rapidly inhibited in hepatocytes incubated in ß-hydroxybutyrate or fatty acids, and the observed inhibition is closely correlated with the production of ketone bodies. These observations show that ketone bodies play an important role in the regulation of ChREBP activity by restricting ChREBP localization to the cytoplasm, thus inhibiting fat synthesis during periods of ketosis.

Structural characterization of a unique interface between carbohydrate response element-binding protein (ChREBP) and 14-3-3ß protein.

Carbohydrate response element-binding protein (ChREBP) is an insulin-independent, glucose-responsive transcription factor that is expressed at high levels in liver hepatocytes where it plays a critical role in converting excess carbohydrates to fat for storage. In response to fluctuating glucose levels, hepatic ChREBP activity is regulated in large part by nucleocytoplasmic shuttling of ChREBP protein via interactions with 14-3-3 proteins. The N-terminal ChREBP regulatory region is necessary and sufficient for glucose-responsive ChREBP nuclear import and export. Here, we report the crystal structure of a complex of 14-3-3ß bound to the N-terminal regulatory region of ChREBP at 2.4 Å resolution. The crystal structure revealed that the α2 helix of ChREBP (residues 117-137) adopts a well defined α-helical conformation and binds 14-3-3 in a phosphorylation-independent manner that is different from all previously characterized 14-3-3 and target protein-binding modes. ChREBP α2 interacts with 14-3-3 through both electrostatic and van der Waals interactions, and the binding is partially mediated by a free sulfate or phosphate. Structure-based mutagenesis and binding assays indicated that disrupting the observed 14-3-3 and ChREBP α2 interface resulted in a loss of complex formation, thus validating the novel protein interaction mode in the 14-3-3ß·ChREBP α2 complex.

Importin-alpha protein binding to a nuclear localization signal of carbohydrate response element-binding protein (ChREBP).

Carbohydrate response element-binding protein (ChREBP) is a glucose-responsive transcription factor that plays a critical role in the glucose-mediated induction of genes involved in hepatic glycolysis and lipogenesis. Circulating blood glucose levels affect ChREBP activity in hepatocytes largely by post-translational mechanisms that include phosphorylation-dependent subcellular localization. Previously, we showed that ChREBP is retained in the cytosol by phosphorylation-dependent binding to 14-3-3 protein dimers and identified the α2 helix (residues 125-135) phospho-Ser(140) domain as the primary 14-3-3 binding site (Sakiyama, H., Wynn, R. M., Lee, W. R., Fukasawa, M., Mizuguchi, H., Gardner, K. H., Repa, J. J., and Uyeda, K. (2008) J. Biol. Chem. 283, 24899-24908). To enter the nucleus in response to high glucose, ChREBP must bind importin-α; this heterodimer then forms a complex with importin-ß to interact with the nuclear pore complex. In this work, we recharacterized the importin-α binding nuclear localization signal (NLS) of rat ChREBP, identifying it as an extended classical bipartite NLS encompassing minimally residues 158-190. Replacing Lys(159)/Lys(190) residues of ChREBP with alanine resulted in loss of importin-α binding, glucose-stimulated transcriptional activity and nuclear localization. A secondary 14-3-3 protein binding site also was identified, the α3 helix (residues 170-190) phospho-Ser(196) domain. Importin-α and 14-3-3 were found to bind competitively to this secondary site. These results suggest an important mechanism by which importin-α and 14-3-3 control movement of ChREBP in and out of the nucleus in response to changes in glucose levels in liver and thus further suggest that the extended NLS of ChREBP is a critical glucose-sensing, glucose-responsive site.

Dual-Path Residual "Shrinkage" Network for Side-Scan Sonar Image Classification.

The underwater environment is complicated and changeable and contains many noises, making it difficult to detect a particular object in the underwater environment. At present, the main seabed detection technology explores the seabed environment with sonar equipment. However, the characteristics of underwater sonar imaging (e.g., low contrast, blurred edges, poor texture, and unsatisfactory quality) have serious negative influences on such image classification. Therefore, in this study, we propose a dual-path deep residual "shrinkage" network (DP-DRSN) module, which is a simple and effective neural network attention module that can classify side-scan sonar images. Specifically, the module can extract background and feature texture information of the input feature mapping through different scales (e.g., global average pooling and global max pooling), whereas scale information passes through a two-layer 1 × 1 convolution to increase nonlinearity. This helps realize cross-channel information interaction and information integration simultaneously before outputting threshold parameters in a sigmoid layer. The parameters are then multiplied by the average value of the input feature mapping to obtain a threshold, which is used to denoise the image features using the soft threshold function. The proposed DP-DRSN study provided higher classification accuracy and efficiency than other models. In this way, the feasibility and effectiveness of DP-DRSN in image classification of side-scan sonar are proven.

Endogenous stress-related signal directs shoot stem cell fate in Arabidopsis thaliana.

Stem cell populations in all multicellular organisms are situated in a niche, which is a special microenvironment that defines stem cell fate. The interplay between stem cells and their niches is crucial for stem cell maintenance. Here, we show that an endogenous stress-related signal (ESS) is overrepresented in the shoot stem cell niche under natural growth conditions, and the vast majority of known stem-cell-specific and niche-specific genes responded to stress signals. Interference with the ESS in the stem cell niche by blocking ethylene signalling impaired stem cell maintenance. Ethylene-insensitive 3 (EIN3), the key transcription factor in ethylene signalling, directly actives the expression of the stress hub transcription factor AGAMOUS-LIKE 22 (AGL22) in the stem cell niche and relays ESS signals to the WUSCHEL/CLAVATA network. Our results provide a mechanistic framework for ESS signalling control of the stem cell niche and demonstrate that plant stem cells are maintained by a native stress microenvironment in vivo.

Structural and functional diversity of bacterial membrane fusion proteins.

Membrane Fusion Proteins (MFPs) are functional subunits of multi-component transporters that perform diverse physiological functions in both Gram-positive and Gram-negative bacteria. MFPs associate with transporters belonging to Resistance-Nodulation-cell Division (RND), ATP-Binding Cassette (ABC) and Major Facilitator (MF) superfamilies of proteins. Recent studies suggested that MFPs interact with substrates and play an active role in transport reactions. In addition, the MFP-dependent transporters from Gram-negative bacteria recruit the outer membrane channels to expel various substrates across the outer membrane into external medium. This review is focused on the diversity, structure and molecular mechanism of MFPs that function in multidrug efflux. Using phylogenetic approaches we analyzed diversity and representation of multidrug MFPs in sequenced bacterial genomes. In addition to previously characterized MFPs from Gram-negative bacteria, we identified MFPs that associate with RND-, MF- and ABC-type transporters in Gram-positive bacteria. Sequence analyses showed that MFPs vary significantly in size (200-650 amino acid residues) with some of them lacking the signature alpha-helical domain of multidrug MFPs. Furthermore, many transport operons contain two- or three genes encoding distinct MFPs. We further discuss the diversity of MFPs in the context of current views on the mechanism and structure of MFP-dependent transporters.

PEG1000-DAIL / Toluene Temperature-dependent Biphasic System that Regulate Homogeneously Catalyzed Oxidation of Primary Alcohols to Carboxylic Acids.

An efficient and convenient procedure for the hydrogen peroxide oxidation of primary alcohols to the carboxylic acids in aqueous media in regulated temperature-dependant; recyclable phase-separation catalytic system comprised of the PEG1000-based dicationic acidic ionic liquid and toluene under homogeneous conditions in good to excellent yield is reported.