RESUMO
In this study we report on some lines of ongoing research performed in our laboratory, in relation to the increased expression of FcR on tumor cells, as well as on cells present in the tumor-bearing host, and its possible role in tumor progression. In a previous study we have shown that a Polyoma virus (PyV)-induced anaplastic carcinoma (SEYF-a tumor) contained an FcR-expressing subpopulation of tumorigenic cells. We tested the effect of in vivo passaging of FcR-expressing and of non-FcR-expressing sub-populations of SEYF-a tumor cells on the expression of FcR, as revealed by the ability of these cells to bind the 2.4G2 monoclonal antibody, which is directed against mouse Fc gamma 2b/gamma 1R. It was found that upon in vivo passaging these two sub-populations became practically identical in their ability to bind anti-Fc gamma R antibody. On the other hand, in vitro passaging of FcR-expressing SEYF-a cells resulted in a gradual decrease in the expression of Fc gamma R. These results, indicating that the expression of Fc gamma R on tumor cells, per se, is dependent on a factor present in the in vivo environment were confirmed using 3T3 cells transformed in vitro by PyV (C) and forming tumors at first injection to mice (CTC). C cultures of various clones did not express Fc gamma R, while CTC cultures (cultures from tumors) became positive. We also detected an increase in the level of a soluble form of Fc gamma 2b/gamma 1R in the circulation of mice bearing PyV induced tumors. This increase paralleled the appearance of palpable tumors. A similar pattern of increase was observed in mice inoculated with the c-H-ras transformed tumorigenic clone 8/F/5, but not in mice inoculated with non-tumorigenic 3T3 cells. Data published by us show that metastatic breast cancer patients had significantly elevated Fc gamma R levels on their peripheral blood mononuclear cells (PBMC). Experiments presented here indicate a direct correlation between increased Fc gamma R levels on PBMC and tumor mass in colon, ovary and lung metastatic carcinoma patients. The possibility that malignantly transformed cells have the potential to cause proliferation of Fc gamma R expressing T cells was tested. It was found that extract derived from r-H-ras transformed 3T3 cells triggers the proliferation of a T cell hybridoma expressing Fc gamma R.
Assuntos
Antígenos de Diferenciação/análise , Neoplasias/imunologia , Receptores Fc/análise , Animais , Comunicação Celular , Humanos , Leucócitos Mononucleares/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Células-Tronco Neoplásicas/imunologia , Oncogenes , Polyomavirus , Receptores de IgG , Sarcoma Experimental/imunologia , Linfócitos T/imunologia , Infecções Tumorais por Vírus/imunologiaRESUMO
The effect of fluvoxamine on the pharmacokinetics of diazepam and metabolically derived N-desmethyl-diazepam was investigated in eight healthy volunteers. Each subject received a single oral dose of diazepam (10 mg) in a control session and on the fourth day of a 16-day treatment with fluvoxamine maleate (100 to 150 mg daily). Compared with the control session, concurrent fluvoxamine intake was associated with increased mean peak plasma diazepam concentrations (from 108 to 143 ng/ml, geometric means, difference not significant), with a marked reduction in apparent oral diazepam clearance (from 0.40 to 0.14 ml/min/kg; p < 0.01) and with a prolongation in diazepam half-life (from 51 to 118 hours; p < 0.01). Although peak plasma N-desmethyldiazepam levels were similar in the two sessions, the time required for the metabolite to reach a peak was longer during fluvoxamine intake than in the control session (206 versus 62 hours; p < 0.01). N-Desmethyldiazepam area under the plasma concentration-time curve values were also significantly increased during fluvoxamine treatment. These data suggest that fluvoxamine inhibits the biotransformation of diazepam and its active N-demethylated metabolite. The magnitude of this interaction is likely to have considerable clinical significance.
Assuntos
Diazepam/farmacocinética , Fluvoxamina/farmacologia , Adulto , Biotransformação , Diazepam/metabolismo , Feminino , Fluvoxamina/efeitos adversos , Humanos , Masculino , Nordazepam/sangueRESUMO
OBJECTIVE: The objective of this study was to assess the pharmacokinetics of intraperitoneal (IP) administration of the antibiotic combination piperacillin/tazobactam (PIP/TAZ) to patients on chronic ambulatory peritoneal dialysis (CAPD) with and without pseudomonas peritonitis. DESIGN: Open-labeled study. SETTING: The study was carried out in the CAPD unit of Assaf Harofeh Medical Center, Zerifin, Israel. PATIENTS AND METHODS: Six patients participated in the study, 4 had pseudomonas peritonitis, all were given an IP loading dose of 4 g/0.5 g PIP/TAZ. Twenty-four hours after the initial dose, a maintenance dose of 0.5 g/0.0625 g PIP/TAZ was administered with each dialysate exchange for a period of 1 week. The patients without peritonitis received only the loading dose. High performance liquid chromatography was used to determine the concentrations of PIPITAZ in plasma obtained at 0, 30, 60, 90, 120, 360, 480, 600, 720, and 1440 minutes after administration. Samples of the dialysate fluid for determination of PIP/TAZ concentration were collected at 6,10,14, 24, and 72, 120, and 168 hours. RESULTS: After the loading dose, the highest plasma PIP concentration (Cmax) was 51.6 t 21.25 Lig/mL and appeared at 1.5 = 0.45 hours (t,,a). During the maintenance period plasma PIP concentration was 5.2 t 4.75 Lg/mL. Tazobactam was detected in the plasma of 1 patient only. The concentration of TAZ in the dialysate fluid during the maintenance period was 2.3 t 0.5 ig/mL. CONCLUSIONS: Piperacillin administered IP at 4 g reached plasma concentrations comparable to intravenous administration and considered therapeutic (above the MIC90 for Pseudomonas aeruginosa) in CAPD patients with or without peritonitis. The maintenance dose, however, should be augmented. Tazobactam could not be detected in the plasma of most patients and the therapeutic implications of IP administration of TAZ cannot be directly correlated to intravenous administration.
Assuntos
Ácido Penicilânico/análogos & derivados , Penicilinas/farmacocinética , Diálise Peritoneal Ambulatorial Contínua , Peritonite/metabolismo , Peritonite/microbiologia , Piperacilina/farmacocinética , Infecções por Pseudomonas/metabolismo , Inibidores de beta-Lactamases , Idoso , Humanos , Masculino , Pessoa de Meia-Idade , Ácido Penicilânico/farmacocinética , TazobactamRESUMO
This paper presents the development of soft clustering and learning vector quantization (LVQ) algorithms that rely on multiple weighted norms to measure the distance between the feature vectors and their prototypes. Clustering and LVQ are formulated in this paper as the minimization of a reformulation function that employs distinct weighted norms to measure the distance between each of the prototypes and the feature vectors under a set of equality constraints imposed on the weight matrices. Fuzzy LVQ and clustering algorithms are obtained as special cases of the proposed formulation. The resulting clustering algorithm is evaluated and benchmarked on three data sets that differ in terms of the data structure and the dimensionality of the feature vectors. This experimental evaluation indicates that the proposed multinorm algorithm outperforms algorithms employing the Euclidean norm as well as existing clustering algorithms employing weighted norms.
RESUMO
Presents a systematic approach for constructing reformulated radial basis function (RBF) neural networks, which was developed to facilitate their training by supervised learning algorithms based on gradient descent. This approach reduces the construction of radial basis function models to the selection of admissible generator functions. The selection of generator functions relies on the concept of the blind spot, which is introduced in the paper. The paper also introduces a new family of reformulated radial basis function neural networks, which are referred to as cosine radial basis functions. Cosine radial basis functions are constructed by linear generator functions of a special form and their use as similarity measures in radial basis function models is justified by their geometric interpretation. A set of experiments on a variety of datasets indicate that cosine radial basis functions outperform considerably conventional radial basis function neural networks with Gaussian radial basis functions. Cosine radial basis functions are also strong competitors to existing reformulated radial basis function models trained by gradient descent and feedforward neural networks with sigmoid hidden units.
RESUMO
The concomitant administration to broilers of ionophore coccidiostats and certain chemotherapeutic agents may cause deleterious interactions, with toxicosis and death as possible sequelae. In this study, co-administration of the ionophore monensin was not shown to alter blood levels of enrofloxacin or norfloxacin. In addition, exposure to lasalocid was not shown to change blood levels of enrofloxacin. However, norfloxacin + lasalocid co-administration induced aminopyrine N-demethylase (AD) activity by day 5 after the last administration of norfloxacin, and induced a rise of norfloxacin levels in the blood. This rise of blood norfloxacin levels after co-administration of norfloxacin + lasalocid implies that lower levels of norfloxacin could be administered in birds also receiving lasalocid.
Assuntos
Anti-Infecciosos/farmacologia , Galinhas/metabolismo , Coccidiostáticos/farmacologia , Sistema Enzimático do Citocromo P-450/efeitos dos fármacos , Fluoroquinolonas , Aminopirina N-Desmetilase/efeitos dos fármacos , Aminopirina N-Desmetilase/metabolismo , Anilina Hidroxilase/efeitos dos fármacos , Anilina Hidroxilase/metabolismo , Animais , Anti-Infecciosos/administração & dosagem , Anti-Infecciosos/sangue , Coccidiostáticos/administração & dosagem , Sistema Enzimático do Citocromo P-450/metabolismo , Interações Medicamentosas , Enrofloxacina , Lasalocida/farmacologia , Masculino , Microssomos Hepáticos/enzimologia , Monensin/farmacologia , Norfloxacino/administração & dosagem , Norfloxacino/sangue , Norfloxacino/farmacologia , Quinolonas/administração & dosagem , Quinolonas/sangue , Quinolonas/farmacologiaRESUMO
The influence of monensin + sulfadimethoxine on cytochrome P-450 monooxygenase activity in broilers, and the possible consequences of modification of this system, including changes in blood levels of sulfadimethoxine, influence on the duration of xylazine-ketamine anesthesia, total antioxidant status and superoxide dismutase activity were studied. The results indicate that the combination of monensin + sulfadimethoxine gave a short-term inhibition of microsomal cytochrome P-450 monooxygenase activity but apparently did not influence the metabolism of other (exogenic) substances (ketamine, xylazine), and did not change the state of antioxidant systems or the relative liver weight. There was a rise in blood sulfadimethoxine levels.
Assuntos
Anti-Infecciosos/farmacologia , Galinhas/metabolismo , Inibidores das Enzimas do Citocromo P-450 , Monensin/farmacologia , Sulfadimetoxina/farmacologia , Animais , Antioxidantes/metabolismo , Coccidiostáticos/farmacologia , Sistema Enzimático do Citocromo P-450/metabolismo , Interações Medicamentosas , Fígado/anatomia & histologia , Masculino , Microssomos Hepáticos/enzimologia , Tamanho do Órgão/efeitos dos fármacosRESUMO
Use of milk urea (MU) concentration as a parameter for detection of nutritional imbalances requires identification and quantification of nutritional and nonnutritional factors that influence it. The objective of this study was to assess the relationship between live body weight (BW) and MU concentration in Holstein cows. Results for the test-day measurements at 7 dairy farms were obtained from the Israeli Dairy Herd Improvement Center and concomitant cow weights were registered in local computerized weighing systems. A total of 1996 cows and 25,485 records were studied. The overall unadjusted per-cow mean MU nitrogen concentration and BW were 15.3 mg/dL (SD = 3.8) and 593 kg (SD = 84), respectively. The linear association between BW and MU was negative and highly significant and the quadratic component of BW had a highly significant positive association with MU. There was a significant interaction between the association of MU and BW with lactation number. Sampling month, milk yield, milk fat percentage, and somatic cell count accounted for significant variation in MU. Predicted MU concentrations at different BW values were calculated for each parity group, by setting equations that included the estimates of the variables associated with MU and constant values (lactation averages) for the independent variables, with the exception of BW. Plotting of results showed exponential characteristics for the relationship between BW and predicted MU concentrations. At any of the considered BW, predicted MU concentrations were lower for first-parity cows. The trends and interactions found in the present study may contribute to improving accuracy of models designed to calculate urinary nitrogen excretion rates and normative milk urea concentrations.
Assuntos
Peso Corporal , Bovinos/fisiologia , Leite/química , Ureia/análise , Animais , Contagem de Células , Feminino , Lactação , Lipídeos/análise , Leite/citologia , Proteínas do Leite/análise , ParidadeRESUMO
Three youngsters aged 12, 16, and 18 years underwent surgery for carcinoma of the colon, a very rare occurrence in this age-group. In all three patients the prominent feature was a marked delay in diagnosis and treatment, as reflected in the advanced pathological stage of the tumors (Dukes' C and D). An increased awareness of the possible occurrence of this type of tumor in young patients, and the resultant earlier diagnosis, are the only means for improving the prognosis.
Assuntos
Adenocarcinoma , Neoplasias Colorretais , Adenocarcinoma/diagnóstico , Adenocarcinoma/mortalidade , Adenocarcinoma/cirurgia , Adolescente , Fatores Etários , Criança , Colectomia , Neoplasias Colorretais/diagnóstico , Neoplasias Colorretais/mortalidade , Neoplasias Colorretais/cirurgia , Colostomia , Feminino , Humanos , Masculino , Estadiamento de Neoplasias , Prognóstico , Taxa de Sobrevida , Fatores de Tempo , Resultado do TratamentoRESUMO
The pharmacokinetics of norfloxacin nicotinate were investigated in unweaned and weaned calves. Following intravenous administration of 7.5 and 15 mg/kg (calculated as norfloxacin base) the clearance values were 8.5 +/- 2.0 or 7.7 +/- 1.2 mL/min.kg (unweaned calves) and 11.7 +/- 3.2 or 16.1 +/- 3.3 mL/min.kg (weaned calves). Norfloxacin mean residence time and volume of distribution values were 211 +/- 33 or 227 +/- 41 min (unweaned calves) and 185 +/- 79 or 128 +/- 18 minutes (weaned calves), and 1.8 +/- 0.3 or 1.7 +/- 0.1 L/kg (unweaned calves) and 2.0 +/- 0.7 or 2.1 +/- 0.7 L/kg (weaned calves) following administration of the lower and higher dose, respectively. These results indicated that norfloxacin pharmacokinetics were similar at a dose range of 7.5-15 mg/kg. However, a significant difference was observed in clearance, mean residence time and the half-life values between the unweaned and weaned calves. The only major pharmacokinetic parameter which did not show a significant difference between the investigated groups was the volume of distribution. The pharmacokinetic differences between the non-ruminating (unweaned) and ruminating (weaned) animals seemed to result from changes in drug clearance. The absorption rate after intramuscular administration appeared to change as a result of dose increase. Norfloxacin bioavailability following intramuscular administration ranged from 73 to 106%. The results suggested that larger injection volumes may reduce the extent of absorption.
Assuntos
Animais Lactentes/metabolismo , Anti-Infecciosos/farmacocinética , Bovinos/metabolismo , Fluoroquinolonas , Norfloxacino/análogos & derivados , Absorção , Animais , Anti-Infecciosos/administração & dosagem , Anti-Infecciosos/sangue , Área Sob a Curva , Sítios de Ligação , Disponibilidade Biológica , Cromatografia Líquida de Alta Pressão , Estudos Cross-Over , Relação Dose-Resposta a Droga , Feminino , Injeções Intramusculares/veterinária , Injeções Intravenosas/veterinária , Ácidos Nicotínicos/administração & dosagem , Ácidos Nicotínicos/sangue , Ácidos Nicotínicos/farmacocinética , Norfloxacino/administração & dosagem , Norfloxacino/sangue , Norfloxacino/farmacocinética , Padrões de Referência , DesmameRESUMO
Single-dose pharmacokinetics of norfloxacin after intravenous administration of norfloxacin nicotinate at 10 mg norfloxacin/kg body weight was investigated in cows with healthy udders and in cows with chronic subclinical and postacute clinical mastitis. An HPLC method was used to determine the norfloxacin concentrations in serum and milk. Significant differences were observed in norfloxacin pharmacokinetics when administered to cows with infected udder quarters. The clearance (Cl) values were 10.4+/-2.5, 13.2+/-1.9 and 14.2+/-2.1 mL/min/kg (mean +/-SD) in the control (healthy udder) cows and in cows with subclinical and postacute clinical mastitis, respectively. There appeared to be a trend of increasing clearance according to severity of the disease. The volume of distribution at steady state (Vss) in the respective groups was 3.1+/-0.7, 2.2+/-0.6 and 1.3+/-0.2 L/kg. The volume of distribution was significantly decreased in the cows with postacute clinical mastitis. The half-lives (t1/2) and mean residence times (MRT) of norfloxacin were 353, 206 and 115 min (harmonic means) and 306+/-76, 168+/-39 and 95+/-9 min in control cows or in cows with subclinical and postacute clinical mastitis, respectively. The half-lives in the clinical mastitis group were significantly shorter than in the control group and the mean residence times were significantly shorter in the two mastitis groups when compared to the control group. Norfloxacin concentrations in milk were extremely high when compared to the respective serum concentrations. The area under the concentration vs. time curve (AUC) of norfloxacin in milk was 23899+/-6206 mg/L x min in the control cow group. The AUC in milk was significantly lower in the infected udder quarters of the mastitis groups (5075+/-1887 mg/L x min and 7484+/-4645 mg/L x min in the subclinical and the clinical group). The AUC values were significantly lower in milk from the infected udder quarters of the cows with chronic subclinical and postacute clinical mastitis when compared to the values in milk from the healthy quarters of the same udder. Norfloxacin was marginally bound to serum protein. The binding was concentration dependent and was 19, 13 and 6% at 0.2, 1.0 and 8.4 mg/L, respectively. Binding to milk protein was 46-51% and concentration independent. An in vitro dialysis model was used to simulate drug transport between serum and milk as a function of protein binding. The results showed that the rate of norfloxacin disposition from milk to serum was slower than from serum to milk, which was in agreement with the findings obtained in the pharmacokinetic study. Norfloxacin was poorly soluble in organic solvents and our results suggest that changes in the degree of ionization of the drug in different body fluids considerably affect its disposition.
Assuntos
Anti-Infecciosos/farmacocinética , Bovinos/metabolismo , Lactação/metabolismo , Mastite Bovina/metabolismo , Norfloxacino/farmacocinética , Animais , Anti-Infecciosos/administração & dosagem , Anti-Infecciosos/uso terapêutico , Área Sob a Curva , Proteínas Sanguíneas/metabolismo , Bovinos/sangue , Cromatografia Líquida de Alta Pressão/veterinária , Infecções por Escherichia coli/tratamento farmacológico , Infecções por Escherichia coli/metabolismo , Infecções por Escherichia coli/veterinária , Feminino , Injeções Intravenosas/veterinária , Mastite Bovina/tratamento farmacológico , Leite/metabolismo , Proteínas do Leite/metabolismo , Norfloxacino/administração & dosagem , Norfloxacino/uso terapêutico , Ligação Proteica , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/metabolismo , Infecções Estafilocócicas/veterináriaRESUMO
In a three-way crossover trial, six healthy Finnish-Merino-Awassi ewes were given a single intravenous injection of norfloxacin nicotinate (in a dose equivalent to 25 mg of norfloxacin base per kg of body weight) during nursing, 1 day after weaning, and 1 month after weaning. Blood and milk samples were collected at different time intervals following dosing, and norfloxacin concentrations were determined by a high-performance liquid chromatography assay. The serum drug concentration versus time data were analyzed by a noncompartmental approach which was based on the statistical-moment theory. The total body clearance values were 4.2 +/- 1.3 (injection during nursing), 1.6 +/- 0.3 (injection 1 day after weaning), and 3.1 +/- 0.8 ml/min/kg (injection 1 month after weaning). The mean residence times were 335 +/- 83, 797 +/- 129, and 481 +/- 102 min and terminal half-lives were 266 +/- 51, 603 +/- 94, and 372 +/- 68 min for the respective treatments. The estimated volumes of distribution at steady state were 1.3 +/- 0.1, 1.2 +/- 0.1, and 1.4 +/- 0.2 liter/kg for the respective treatments. Milk norfloxacin concentrations were up to 40 times higher than the corresponding concentrations in serum during lactation. Accordingly, in ewes with 1.5 liter of milk in the udder more than half of the drug in the animal appeared to be in the milk. Therapeutic concentrations of norfloxacin could be detected in the sera of suckling lambs, implicating that fluoroquinolone therapy should be discouraged during breast feeding. In lactating ewes and in ewes with full udders, moment analysis calculations did not show a significant difference between the system moment mean residence time and the system matrix mean residence time values. Thus, the pharmacokinetics of norfloxacin in the three groups could be described by the classical two-compartment open-body model with input and output occurring from the central compartment. The results did not support the existence of a distinguishable milk compartment. Milk secretion seemed to act as one of the clearance processes of the drug when milk was continuously removed.
Assuntos
Anti-Infecciosos/farmacocinética , Fluoroquinolonas , Leite/metabolismo , Norfloxacino/análogos & derivados , Ovinos/metabolismo , Animais , Feminino , Lactação , Taxa de Depuração Metabólica , Ácidos Nicotínicos/farmacocinética , Norfloxacino/farmacocinéticaRESUMO
Three clinically normal pigs were given a single intramuscular injection of an aqueous solution of norfloxacin nicotinate (NFN) at 14 mg/kg body weight. Animals were killed 4 h after treatment and the concentrations of norfloxacin in various biological fluids and tissues were determined by chemical (high performance liquid chromatography, HPLC) and microbiological assay methods. Drug distribution throughout the body was presented as actual concentrations (micrograms/ml and ppm) in each sample and as the ratio of drug concentration in tissue to drug concentration in plasma. Highest concentrations were found in the urine, kidney, liver and bile. The drug was not detected in ocular fluid, brain tissue (by microbiological assay), fat (by HPLC) and skin (by HPLC). Tissue-to-plasma concentrations ratios were near to, or greater than, 1.0 (HPLC assay) for the kidney, liver, spleen, muscle, lung, adrenals, salivary glands, pleural and synovial fluid, and smaller than 1.0 for cerebrospinal fluid, brain tissue and lymph nodes. Agreement between the chemical and microbiological assay results was variable, depending on the type of tissue and biological fluid tested. Binding of norfloxacin to plasma proteins in pig is low (23%-29%). The distribution pattern of the drug in pig, laboratory animals and humans is very similar; it can be characterized as extensive, with tissue-to-serum ratios reaching 2:1 or more in certain non-excretory organs. These values may reflect intracellular concentrations of the drug.
Assuntos
Anti-Infecciosos/farmacocinética , Proteínas Sanguíneas/metabolismo , Fluoroquinolonas , Norfloxacino/análogos & derivados , Suínos/metabolismo , Animais , Anti-Infecciosos/administração & dosagem , Injeções Intramusculares/veterinária , Masculino , Ácidos Nicotínicos/administração & dosagem , Ácidos Nicotínicos/farmacocinética , Norfloxacino/administração & dosagem , Norfloxacino/farmacocinética , Ligação Proteica , Distribuição TecidualRESUMO
Metronidazole pharmacokinetics in horses was studied after intravenous (i.v.), rectal (p.r.) and oral (p.o.) administration at 20 mg/kg using a triple crossover study design. Metronidazole mean+/-SD half-life was 196+/-39, 212+/-30 and 240+/-65 min after i.v., p.r. and p.o. administration, respectively. The metronidazole clearance was 2.8 (mL/min/kg) and the volume of distribution at steady state was 0.68 L/kg. The pharmacokinetic parameters calculated for metronidazole after administration of the drug by the various routes showed that bioavailability (74+/-18 vs. 30+/-9%) and maximum serum concentration (22+/-8 vs. 9+/-2 microg /mL) were significantly higher after p.o. administration compared with p.r. administration. There were no significant differences in mean absorption time (45+/-69 vs. 66+/-18 min) and the time to reach maximum serum concentration (65+/-36 vs. 58+/-18 min). The results indicated that p.r. administration of metronidazole to horses, although inferior to p.o. administration in terms of bioavailability, provides an alternative route of administration when p.o. administration cannot be used.
Assuntos
Anti-Infecciosos/farmacocinética , Metronidazol/farmacocinética , Absorção , Administração Oral , Administração Retal , Animais , Anti-Infecciosos/administração & dosagem , Anti-Infecciosos/sangue , Área Sob a Curva , Disponibilidade Biológica , Estudos Cross-Over , Meia-Vida , Cavalos , Injeções Intravenosas , Masculino , Metronidazol/administração & dosagem , Metronidazol/sangue , Distribuição TecidualRESUMO
The pharmacokinetics of sulphadoxine-trimethoprim was studied in 6 pre-ruminant calves using two different products. Product A, which contained 200 mg sulphadoxine and 40 mg trimethoprim per mL, was administered intravenously or subcutaneously at a dosage of 25 mg sulphadoxine and 5 mg trimethoprim.kg-1 bodyweight. Product B, containing 62.5 mg sulphadoxine and 12.5 mg trimethoprim per mL plus lidocaine (1 mg.mL-1), was given subcutaneously at the same dosage. After intravenous administration of product A the mean time of half-life of elimination phase (t1/2) for sulphadoxine was 12.9 h, steady-state volume of distribution (Vd(ss)) was 0.44 L.kg-1 and clearance was 0.024 L.kg-1.h-1. Respective values for trimethoprim were 1.9 h, 2.0 L.kg-1 and 0.9 L.kg-1.h-1. After subcutaneous administration, the bioavailability of sulphadoxine was 96% and 98% and the time to reach a maximum concentration was 6.3 and 8.0 h for products A and B, respectively. The Cmax for trimethoprim was higher for product A (0.49 microgram.mL-1) than for product B (0.32 microgram.mL-1) (p = 0.014). Slow absorption from the injection site appeared to delay the elimination of trimethoprim after subcutaneous administration when compared to that after intravenous administration: apparent elimination t1/2 for trimethoprim after intravenous administration of product A was 1.9 h compared to 3.9 h and 3.6 h after subcutaneous administration of products A and B, respectively. The difference between intravenous and subcutaneous administrations was statistically significant (p < 0.05). Also the mean residence time was significantly shorter (p < 0.05) after intravenous administration (2.4 h) than that after subcutaneous administration of product A (6.9 h) and B (7.1 h). The bioavailability of trimethoprim was lower than that of sulphadoxine: 76% and 74% for products A and B, respectively. All 6 calves showed pain after subcutaneous administration of product A and the injection sites were warm and showed soft oedematous reactions 5-8 cm in diameter. Three of the calves also showed some pain after subcutaneous administration of product B; the local reactions were less severe. A marked increase was seen in creatine kinase activity after subcutaneous administration of both products. Product A caused a more pronounced increase but the difference was not statistically significant. We suggest 30 mg.kg-1 at 24-h intervals or alternatively 15 mg.kg-1 at 12-h intervals as the minimum dosage of sulphadoxine-trimethoprim combination for pre-ruminant calves. Extravascular routes of administration should be avoided due to marked tissue irritation at the injection site.
Assuntos
Anti-Infecciosos/farmacocinética , Bovinos/metabolismo , Sulfadoxina/farmacocinética , Trimetoprima/farmacocinética , Animais , Anti-Infecciosos/administração & dosagem , Anti-Infecciosos/efeitos adversos , Disponibilidade Biológica , Creatina Quinase/sangue , Estudos Cross-Over , Combinação de Medicamentos , Injeções Intravenosas/veterinária , Injeções Subcutâneas/veterinária , Pele/efeitos dos fármacos , Sulfadoxina/administração & dosagem , Sulfadoxina/efeitos adversos , Fatores de Tempo , Trimetoprima/administração & dosagem , Trimetoprima/efeitos adversosRESUMO
The objectives of this study were to identify and evaluate production and environmental factors that influence milk urea (MU) in Israeli dairy herds, to analyze the relationships between MU concentration and nutritional variables, and to examine a possible association between MU and pregnancy rate (PR). Production and environmental data were obtained from the Israeli Dairy Herd Improvement (DHI) Center (n = 1,279,600). Programmed total mixed rations (feeds and quantities) on milk-test day were collected from 42 dairy herds. Data on 36,073 cows that were inseminated close to milk-test day and pregnancy diagnosis results were obtained from the DHI data bank. Highly significant positive relationships were found between MU concentration and milk yield and fat percentage; relationships between MU and milk total protein percentage and somatic cell count were negative. Milk urea levels were higher during the summer months and were higher for adult cows. These levels increased as lactation progressed. Milk urea was positively associated with dietary levels of crude protein, ruminal digestible protein, and neutral detergent fiber contents; it was negatively associated with ration energy and nonstructural carbohydrate contents. Significant influences of specific feeds on MU were detected. A significant negative association was found between MU level and PR. Least squares means for PR for cows in the lowest and highest MU quartiles were 38.4 and 36.1%, respectively. Increasing levels of MU were negatively related to reproductive performance of dairy cows, but the risk of nonpregnancy caused by high levels of MU was lower than reported in previous studies.
Assuntos
Fenômenos Fisiológicos da Nutrição Animal , Bovinos/fisiologia , Fertilidade , Lactação , Leite/química , Ureia/análise , Ração Animal , Animais , Contagem de Células , Fibras na Dieta/administração & dosagem , Proteínas Alimentares/administração & dosagem , Meio Ambiente , Feminino , Inseminação Artificial/veterinária , Israel , Lipídeos/análise , Leite/citologia , Proteínas do Leite/análise , GravidezRESUMO
Cisplatin is a widely used chemotherapeutic agent implicated in a range of adverse effects affecting the nervous system. Among the others, convulsive encephalopathy is rare and its pathogenesis is unknown. We report an 84-year-old woman with adenocarcinoma of the ovary who developed two fully reversible episodes of non-convulsive encephalopathy, each following a course of cisplatin-based chemotherapy and thus confirming a causal relationship to the agent. The patient presented 7 and 10 days after treatment with acute confusional state, a partial left homonymous hemianopia and a left extinction hemihypesthesia. Brain MRI showed old-standing cerebral microvascular changes and EEG revealed right parieto-occipital periodic lateralized epileptiform discharges over a generalized background activity slowing. This case adds further to the clinical diversity of cisplatin toxicity and, in view of the similarity to a recently defined disorder of posterior leukoencephalopathy, suggests regional endovascular injury rather than a direct cerebral toxicity as the initial event in the evolution of encephalopathy.
Assuntos
Adenocarcinoma Papilar/tratamento farmacológico , Antineoplásicos/efeitos adversos , Encefalopatias/induzido quimicamente , Cisplatino/efeitos adversos , Neoplasias Ovarianas/tratamento farmacológico , Adenocarcinoma Papilar/sangue , Idoso , Idoso de 80 Anos ou mais , Encefalopatias/diagnóstico , Encefalopatias/terapia , Eletroencefalografia , Feminino , Humanos , Imageamento por Ressonância Magnética , Neoplasias Ovarianas/sangueRESUMO
Grapefruit juice (GJ), a cytochrome P450 (CYP) 3A4 inhibitor, may affect the pharmacokinetics of drugs metabolized through CYP 3A4. Losartan, an angiotensin II antagonist, is converted into its main active metabolite E3174 by CYP 3A4 and CYP 2C9. The effect of GJ on losartan pharmacokinetics was assessed in a randomized crossover trial. Losartan was given to 9 volunteers with and without GJ. Concentrations of losartan and its E3174 metabolite were determined in serum by a high-performance liquid chromatography method (HPLC). Significant differences were observed in some of the pharmacokinetic parameters of losartan and its metabolite E3174 after losartan administration with and without co-administered GJ. The lag time (time to drug appearance in serum) of losartan increased significantly with co-administered GJ. The mean residence time (MRT) and half-life (t(1/2)) of the E3174 metabolite were significantly longer and the area under the concentration--time curve (AUC) of the E3174 metabolite was significantly smaller after concomitant GJ administration. The ratio AUC(losartan)/AUC(E3174) was significantly increased after concurrent grapefruit juice intake. The increased lag time of losartan and the increased MRT and t1/2 and decreased AUC of E3174 were considered indicative of simultaneous CYP 3A4 inhibition and P-glycoprotein activation. The significantly increased AUC(losartan)/AUC(E3174) ratio, however, indicates reduced losartan conversion to E3174 by CYP 3A4 metabolism as a result of co-administered GJ.
Assuntos
Antiarrítmicos/farmacocinética , Anti-Hipertensivos/farmacocinética , Bebidas , Citrus , Imidazóis/farmacocinética , Losartan/farmacocinética , Tetrazóis/farmacocinética , Adulto , Área Sob a Curva , Disponibilidade Biológica , Cromatografia Líquida de Alta Pressão , Estudos Cross-Over , Feminino , Meia-Vida , Humanos , MasculinoRESUMO
OBJECTIVE: To investigate the effect of grapefruit juice (GJ) on the pharmacokinetics of orally administered verapamil in hypertensive patients. METHODS: Ten hypertensive patients on chronic verapamil treatment participated in a two-day study. On day 1 200 ml of water was given 1 hour before, and together with the morning verapamil dose; on the day 2, water was replaced by GJ in the same order. Serial blood samples were collected and the concentrations of verapamil and its main dealkylated metabolite (D-617) were determined by high-performance liquid chromatography (HPLC). The area under the concentration versus time curve of verapamil (AUCv) and its metabolite D-617 (AUCM) were calculated before and after GJ ingestion. The peak serum concentration (Cmax) and the time until its appearance (tmax) were also determined. RESULTS: GJ did not affect Cmax, tmax, AUCv or AUVm. The AUCv/AUCm ratio (AUCR) was slightly, but significantly, increased after GJ (1.67 vs 1.92). CONCLUSIONS: A single administration of GJ with short-acting verapamil has no significant effect on the pharmacokinetics, of verapamil.
Assuntos
Bebidas , Bloqueadores dos Canais de Cálcio/farmacocinética , Citrus , Verapamil/farmacocinética , Administração Oral , Idoso , Idoso de 80 Anos ou mais , Feminino , Interações Alimento-Droga , Humanos , Masculino , Pessoa de Meia-Idade , Verapamil/sangueRESUMO
BACKGROUND: This study evaluated the optimal formulation (tablet vs solution) and frequency (once vs twice daily) of maintenance oral furosemide in compensated congestive heart failure (CHF). METHODS AND RESULTS: Eighteen patients with mild (group 1) and 18 with severe (group 2) CHF were studied. On 2 consecutive days each patient's individual fixed oral furosemide daily dosage was administered in tablet or solution in a crossover design. In an additional study, 14 patients with severe CHF received the daily dosage in tablet or solution form in two divided doses. Pharmacokinetic data were obtained in six randomly allocated patients of each group, during the once daily administration periods of either formulation. Twenty-four-hour urinary sodium and 12-hour volume were significantly greater in group 1 following furosemide solution versus tablets. In group 2, all parameters were comparable in response to single identical doses in tablets or solution, as well as to once versus twice daily administration of either formulation. These results coincided with a higher C(max) and a shorter t(max) following solution. CONCLUSIONS: A once-daily oral furosemide solution is more effective than the same dosage in tablet form in patients with mild, but not those with severe, CHF. In patients with severe CHF, the natriuretic and diuretic effects are similar whether oral furosemide in tablet or solution is administered in a once or twice daily schedule.