Body size and social status in medieval Alba (Cuneo), Italy.

OBJECTIVES: Previous work by Vercellotti et al. in 2011 found significant status-related differences in body size in males but not in females from the Italian bioarchaeological assemblage of San Michele di Trino (8th-14th centuries CE). The purpose of the present work is twofold: (a) to determine if status-related body size differences could be observed in the nearby collection of San Lorenzo di Alba (7th-15th centuries CE) and (b) to add to the emerging narrative of medieval Italians. MATERIALS AND METHODS: Osteometric data (maximum length for the humerus, radius, ulna, femur, tibia, and fibula; bicondylar length of the femur, condylo-malleolar length of the tibia, foot height, maximum vertebral heights, and basion-bregma height) were collected for 50 (20 female, 30 male) individuals from Alba, and Monte Carlo analysis was used to assess differences in skeletal element size, skeletal height, living stature, and body mass across sex and status. RESULTS: Significant differences were detected between high status and low status males in Alba for radial maximum length (p = 0.013), tibial maximum length (p = 0.011), tibial condylo-malleolar length (p = 0.012), skeletal height estimated from condylo-malleolar tibial length (p = 0.002), and stature estimated from condyle-malleolar tibial length with the age component (p = 0.003). In contrast, no significant status-based differences were observed between female subsamples (p > 0.05). DISCUSSION: The patterns of intrapopulation variation observed at Alba are similar but not as pronounced as those observed at Trino, suggesting that overall life conditions experienced by the two groups were comparable.

Induction of chromosomal aberrations and micronuclei by 2-hydroxy-4-methoxybenzophenone (oxybenzone) in human lymphocytes.

Oxybenzone or benzophenone-3 (2-hydroxy-4-methoxybenzophenone; BP-3) is a filter used in a variety of personal care products for protection of human skin and hair from damage by ultraviolet radiation. BP-3 is suspected to exhibit endocrine disruptive properties. Indeed, it was found to be able to interact with the endocrine system causing alteration of its homeostasis, with consequent adverse health effects. Moreover, it is ubiquitously present in the environment, mostly in aquatic ecosystems, with consequent risks to the health of aquatic organisms and humans. In the present study, we analyzed the cytogenetic effects of BP-3 on human lymphocytes using in vitro chromosomal aberrations and micronuclei assays. Blood samples were obtained from five healthy Italian subjects. Lymphocyte cultures were exposed to five concentrations of BP-3 (0.20, 0.10, 0.05, 0.025, and 0.0125 µg/mL) for 24 and 48 h (for chromosomal aberrations and micronuclei tests, respectively). The concentration of 0.10 µg/mL represents the acceptable/tolerable daily intake reference dose established by European Union, whereas 0.20, 0.05, 0.025, and 0.0125 µg/mL represent multiple and sub-multiple of this concentration value. Our results reported cytogenetic effects of BP-3 on cultured human lymphocytes in terms of increased micronuclei and chromosomal aberrations' frequencies at all tested concentrations, including concentrations lower than those established by European Union. Vice versa, after 48-h exposure, a significant reduction of the cytokinesis-block proliferation index value in cultures treated with BP-3 was not observed, indicating that BP-3 does not seem to produce effects on the proliferation/mitotic index when its concentration is equal to or less than 0.20 µg/mL.