RESUMO
As most mechanisms of adaptive immunity evolved during the divergence of vertebrates, the immune systems of extant vertebrates represent different successful variations on the themes initiated in their earliest common ancestors. The genes involved in elaborating these mechanisms have been subject to exceptional selective pressures in an arms race with highly adaptable pathogens, resulting in highly divergent sequences of orthologous genes and the gain and loss of members of gene families as different species find different solutions to the challenge of infection. Consequently, it has been difficult to transfer to the chicken detailed knowledge of the molecular mechanisms of the mammalian immune system and, thus, to enhance the already significant contribution of chickens toward understanding the evolution of immunity. The availability of the chicken genome sequence provides the opportunity to resolve outstanding questions concerning which molecular components of the immune system are shared between mammals and birds and which represent their unique evolutionary solutions. We have integrated genome data with existing knowledge to make a new comparative census of members of cytokine and chemokine gene families, distinguishing the core set of molecules likely to be common to all higher vertebrates from those particular to these 300 million-year-old lineages. Some differences can be explained by the different architectures of the mammalian and avian immune systems. Chickens lack lymph nodes and also the genes for the lymphotoxins and lymphotoxin receptors. The lack of functional eosinophils correlates with the absence of the eotaxin genes and our previously reported observation that interleukin- 5 (IL-5) is a pseudogene. To summarize, in the chicken genome, we can identify the genes for 23 ILs, 8 type I interferons (IFNs), IFN-gamma, 1 colony-stimulating factor (GM-CSF), 2 of the 3 known transforming growth factors (TGFs), 24 chemokines (1 XCL, 14 CCL, 8 CXCL, and 1 CX3CL), and 10 tumor necrosis factor superfamily (TNFSF) members. Receptor genes present in the genome suggest the likely presence of 2 other ILs, 1 other CSF, and 2 other TNFSF members.
Assuntos
Quimiocinas/genética , Galinhas/genética , Citocinas/genética , Genômica , Sequência de Aminoácidos , Animais , Citocinas/química , Humanos , Inflamação/genética , Dados de Sequência Molecular , Filogenia , Receptores de Quimiocinas/genética , Alinhamento de SequênciaRESUMO
Based upon the recognition of antiviral compounds and single stranded viral RNA the Toll-like receptors TLR7 and TLR8 are suggested to play a significant role in initiating antiviral immune responses. Here we report the molecular characterization of the chicken TLR7/8 loci which revealed an intact TLR7 gene and fragments of a TLR8-like gene with a 6-kilobase insertion containing chicken repeat 1 (CR1) retroviral-like insertion elements. The chicken TLR7 gene encodes a 1047-amino-acid protein with 62% identity to human TLR7 and a conserved pattern of predicted leucine-rich repeats. Highest levels of chicken TLR7 mRNA were detected in immune-related tissues and cells, especially the spleen, caecal, tonsil and splenic B cells. Alternative spliced forms of TLR7 mRNA were identified in chicken, mouse and human and expressed in similar tissues and cell types to the major form of chicken TLR7. The chicken TLR7+ HD11 cell line and fresh splenocytes produced elevated levels of interleukin-1beta (IL-1beta) mRNA after exposure to the agonists R848 and loxoribine. Interestingly, none of the TLR7 agonists stimulated increased type I interferon (IFN) mRNA whereas poly(I:C) (a TLR3 agonist) up-regulated both chicken IFN-alpha and chicken IFN-beta mRNA. In contrast, TLR7 agonists, particularly R848 and poly(U) stimulated up-regulation of chicken IL-1beta, and chicken IL-8 mRNAs more effectively than poly(I:C). Stimulation of chicken TLR7 with R848 was chloroquine sensitive, suggesting signalling within an endosomal compartment, as for mammalian TLR7. The deletion of TLR8 in galliforms, accompanied with the differential response after exposure to TLR7 agonists, offers insight into the evolution of vertebrate TLR function.
Assuntos
Linfócitos B/química , Guanosina/análogos & derivados , Glicoproteínas de Membrana/genética , RNA Mensageiro/análise , Receptores de Superfície Celular/genética , Viroses/imunologia , Sequência de Aminoácidos , Animais , Linfócitos B/imunologia , Ceco/imunologia , Linhagem Celular , Galinhas , Sequência Conservada , Guanosina/farmacologia , Humanos , Imidazóis/farmacologia , Hibridização in Situ Fluorescente , Indutores de Interferon/farmacologia , Interferon-alfa/metabolismo , Interferon beta/metabolismo , Interleucina-1/genética , Glicoproteínas de Membrana/agonistas , Camundongos , Dados de Sequência Molecular , Tonsila Palatina/química , Poli I-C/farmacologia , Dobramento de Proteína , Receptores de Superfície Celular/agonistas , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Alinhamento de Sequência , Organismos Livres de Patógenos Específicos , Baço/imunologia , Estimulação Química , Receptor 3 Toll-Like , Receptor 7 Toll-Like , Receptor 8 Toll-Like , Receptores Toll-LikeRESUMO
Toll-like receptors (TLRs) are a major component of the pattern recognition receptor repertoire that detect invading microorganisms and direct the vertebrate immune system to eliminate infection. In chickens, the differential biology of Salmonella serovars (systemic versus gut-restricted localization) correlates with the presence or absence of flagella, a known TLR5 agonist. Chicken TLR5 (chTLR5) exhibits conserved sequence and structural similarity with mammalian TLR5 and is expressed in tissues and cell populations of immunological and stromal origin. Exposure of chTLR5+ cells to flagellin induced elevated levels of chicken interleukin-1beta (chIL-1beta) but little upregulation of chIL-6 mRNA. Consistent with the flagellin-TLR5 hypothesis, an aflagellar Salmonella enterica serovar Typhimurium fliM mutant exhibited an enhanced ability to establish systemic infection. During the early stages of infection, the fliM mutant induced less IL-1beta mRNA and polymorphonuclear cell infiltration of the gut. Collectively, the data represent the identification and functional characterization of a nonmammalian TLR5 and indicate a role in restricting the entry of flagellated Salmonella into systemic sites of the chicken.
Assuntos
Glicoproteínas de Membrana/fisiologia , Receptores de Superfície Celular/fisiologia , Salmonelose Animal/imunologia , Salmonella typhimurium/imunologia , Sequência de Aminoácidos , Animais , Galinhas , Flagelina/metabolismo , Interleucina-1/genética , Interleucina-6/genética , Glicoproteínas de Membrana/química , Glicoproteínas de Membrana/genética , Dados de Sequência Molecular , RNA Mensageiro/análise , Receptores de Superfície Celular/química , Receptores de Superfície Celular/genética , Receptor 5 Toll-Like , Receptores Toll-LikeRESUMO
CD4 has a central role in thymocyte differentiation and cell-mediated immunity. We isolated and analyzed chicken CD4. The gene spans 11.5 kb and is composed of ten exons. The promoter is TATA-less and similar to the mouse and human CD4 promoters, with two transcription start sites as determined by 5'RACE analysis. In general the introns are short, although the 5'untranslated region includes a large intron of 5.6 kb containing binding sites of the putative CD4 silencer. The single-strand conformation polymorphism technique was used to identify a polymorphism to map the gene, which lies on chicken Chromosome 1 in a position showing conserved synteny to mouse and human. This is the first report describing the organization of CD4 from a non-mammalian species. The structure and localization of chicken CD4 and many sequence motifs important in its regulation have remained conserved during evolution.