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The structure and solvation behavior of binary liquid mixtures of Methanol (MeOH) and N, N-Dimethylformamide (DMF) are explored by ascertaining their intermolecular interactions with either Rhodamine-B (RhB) or Rhodamine101 (Rh101) dye through steady-state absorption, emission, and two-photon induced fluorescence. Specifically, in the present investigation, we examine the strong synergistic solvation observed for the combinations of hydrogen bond donating (MeOH) and accepting (DMF) solvent pairs. Solvatochromism causes the solvatochromic probe molecules to sense increased polarity compared to their bulk counterparts. The origin of synergism was explained in terms of solute-solvent and solvent-solvent interactions in binary solvent mixtures interactions, as evidenced by probe dependence. The solvation behavior of the Methanol and DMF binary solvent mixture shows strong probe dependence, with Rh101 showing synergism while RhB does not.
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INTRODUCTION: Prior estimates of dementia prevalence in India were based on samples from selected communities, inadequately representing the national and state populations. METHODS: From the Longitudinal Aging Study in India (LASI) we recruited a sample of adults ages 60+ and administered a rich battery of neuropsychological tests and an informant interview in 2018 through 2020. We obtained a clinical consensus rating of dementia status for a subsample (N = 2528), fitted a logistic model for dementia status on this subsample, and then imputed dementia status for all other LASI respondents aged 60+ (N = 28,949). RESULTS: The estimated dementia prevalence for adults ages 60+ in India is 7.4%, with significant age and education gradients, sex and urban/rural differences, and cross-state variation. DISCUSSION: An estimated 8.8 million Indians older than 60 years have dementia. The burden of dementia cases is unevenly distributed across states and subpopulations and may therefore require different levels of local planning and support. HIGHLIGHTS: The estimated dementia prevalence for adults ages 60+ in India is 7.4%. About 8.8 million Indians older than 60 years live with dementia. Dementia is more prevalent among females than males and in rural than urban areas. Significant cross-state variation exists in dementia prevalence.
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Demência , Masculino , Feminino , Humanos , Demência/epidemiologia , Prevalência , Envelhecimento , Testes Neuropsicológicos , Índia/epidemiologiaRESUMO
Fluid systems exhibit thermal lens effects due to laser irradiation accompanied by convection and in contrast primarily conductive heat dissipation is observed in solids. The presence of a significant convective mode modifies the temperature gradient in fluids resulting in a deviation of the experimental results from theories that are based on pure conduction. Herein, we present a carefully designed femtosecond laser experiment that keeps the heat generation process constant in order to account for the effect of molecular properties on thermal dissipation. We derive a theoretical model that introduces and characterizes the additional convective heat transfer in thermal lens (TL) spectroscopy which explains our observed experimental results. We measured the TL signal for a series of liquid aliphatic alkanes, ranging from hexane to decane, and their comparison has proven the validity of our model. The influence of convective heat transfer on the TL signal is predicted in terms of the dimensionless Peclet number (PE). The lower values of PE for alkanes with longer carbon chains indicate that the convective flow of heat slows down substantially for larger molecules.
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This study deals with the effects of different sets of binary solvents on the Two-Photon Induced Fluorescence (TPIF), a non-linear process, of the Rhodamine-6G (Rh6G) dye, which is a well-known xanthene dye. This work examines the importance of inter-molecular interactions, which results in the modulation of the TPIF of the Rh6G. In this work, we have investigated three binary solvent mixtures representing varying polarity and intermolecular interactions. Specific solvent mixtures used are methanol-water, methanol-dimethyl formamide, and methanol-chloroform. Since the solvent polarity across these binary solvents differs, there are significant intermolecular interactions in the binary mixture solvents, which modulate the two-photon process of Rh6G when irradiated with high-intensity laser light at 780 nm. In our studies, we find that Rh6G in the MeOH-H2O binary solvent has maximum red-shift and minimum intensity as compared to other pairs of binary liquids when the volume fraction of methanol decreases due to more extensive hydrogen bonding between the two components. Additionally, at 1:1 ratio of binary mixtures, Rh6G is found to have the highest TPEACS value for methanol-chloroform binary solvent and reason for that is related to the formation of weak H-bond networks between proton donor chloroform and proton acceptor methanol.
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A facile synthesis and detailed photophysical investigation of E/Z-isomerization of fluorescent diphenylamine tethered stilbene derivatives (DPASs) under white light exposure have been carried out to understand the effect on fluorescence, electrochemical properties, and photostability under various activation/deactivation pathways. In solution state, in the dark, the E-isomer of DPASs (6a-d) exhibited high fluorescence quantum yields (Φfl ≈ 53% to 60% in DMSO). However, on white light exposure, 1H NMR and HPLC studies revealed that pure E-isomer of the DPAS 6a (â¼9.5 mM) started converting into its Z-form by photoisomerization until it reaches to nearly equilibrium. At low concentrations (â¼10 µM), the absorption band of the pure E-isomer in the range of 350-450 nm gradually decreased to adopt Z-conformation 6a' until a photostationary state was reached. The structure of the E-isomer 6a was unequivocally confirmed by X-ray diffraction analysis. The synthesized DPAS compounds 6a-d possessed positive solvatochromic properties, two photon absorption properties, and good thermal stability. The electrochemical investigations using DPASs showed reversible oxidation resulting in formation of a stable radical cation. Owing to useful photophysical, electrochemical and thermal properties, these DPAS derivatives are suitable for their application in biomedical imaging as well as in fabrication of electroluminescent materials.
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Rhodamine group of molecules are widely used dyes for imaging of biological molecules. Application of these dyes however includes a limitation that these molecules absorb in the visible range of the spectrum, which does not fall in the 'biologically transparent window' (BTW). Two photon absorption (TPA) process could come up with an alternate solution to this as these dyes could be excited in the near infrared (NIR) window to extract similar information. To validate this we have investigated TPA cross section (TPACS, σ2) of two rhodamine dyes, namely Rhodamine 6G (R6G), Rhodamine B (RhB), site selectively bound with a model protein, bovine serum albumin (BSA), by exciting at 800 nm. Two photon spectroscopy and imaging confirms the binding of the dye to the protein. The decreases in TPACS with increasing temperature at a fixed BSA concentration excellently follows the temperature induced structural transition of BSA as the protein transforms from a molten globule to unfolded conformation beyond 60 °C, which has previously been established through circular dichroism (CD) measurements. The thus established resemblance in TPACS and CD measurement trends thus strongly affirms the suitability of TPA process in protein imaging and as an alternative marker to tracking its conformational transformations using NIR radiation.
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Corantes Fluorescentes/química , Fótons , Desnaturação Proteica , Rodaminas/química , Soroalbumina Bovina/química , Conformação Proteica , Espectrometria de FluorescênciaRESUMO
Using a degenerate pump probe technique at 800 nm, Ground State Vibrational Coherence (GSVC) of a cyanine dye (IR780) is explored in various solvents. Five representative solvents were studied, of which GSVC is present in three: methanol (MeOH), ethanol (EtOH) and dichloromethane (DCM). The maximum number of damped oscillations was observed in DCM solvent and the oscillations persisted for 1.2 ps. However, GSVC is absent in two solvents: acetonitrile (ACN) and dimethyl sulfoxide (DMSO). For nondegenerate pump probe studies with a 400 nm pump and a supercontinuum probe, GSVC was absent for IR780 in all of the five solvents studied. Instead these studies showed excited state absorption corresponding to S1 â Sn absorption bands in the 450 to 650 nm range. Decay profiles for these correspond to the higher electronic states (Sn, n ≥ 2) in contrast to the degenerate case involving S1. Complete dynamics of IR780 with 30 fs pulses are reported by invoking three time-constants to fit the kinetic traces that can, in turn, be assigned as: vibrational cooling, intermediate state time, and ground state recovery (GSR). All these dynamics exhibit noticeable solvent dependence. A plateau region is also observed in the early part of the GSR dynamics, strongly suggesting the existence of an intermediate state between the ground (S0) and excited singlet (S1) states.
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Effect of solution pH on two-photon absorption cross-section of highly fluorescent Coumarin and Rhodamine dyes with high repetition rate femtosecond laser pulses at 780 nm is presented using two-photon induced fluorescence technique. A correspondence in the measured two-photon and single-photon cross-section values is seen when the pH changes from acidic to basic conditions (pH = 2-10) for solutions in 1:1 water-ethanol binary mixture. By plotting changes in the single-photon and two-photon fluorescence in this pH range, the excited state pKa values are found. The ground state pKa values are also affected by the protonation deprotonation equilibrium as a result of variation in pH from acidic to basic, which are characterized by changes in absorbance spectra. Most of these single-photon and two-photon induced fluorescence spectra show characteristic blue shifts. Different fluorescence quantum yields calculated at each pH reflect a change in structure corresponding to their associated properties as a result of acid base equilibrium.
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We have investigated the concentration dependent of two-photon induced fluorescence (TPIF) in methanolic solution of Rhodamine 6G and Rhodamine B dye using 120 fs laser pulses at 780 nm, 76 MHz repetition rate. TPIF study of these dyes was compared with their respective one photon fluorescence intensity. We have shown the effect of chopper on TPIF intensity from Rhodamine dyes, which have shown direct influence on the determined TPA Cross section of these dyes.
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We report a structure of ferrocene-pyrene conjugate (1) comprising electro and photo-active dual-signaling units. In particular, 1 upon interaction with Cu(II), displays selectively one-photon fluorescence quenching, but it shows two-photon absorption (TPA) cross-section 1230 GM (at 780â nm). Further, 1 displayed two irreversible oxidative waves at 0.39 V and 0.80 V (vs Ag/AgCl), in the electrochemical analysis which upon addition of Cu2+, led to the negative potential shift in both the oxidative waves to appear at 0.25 V and 0.68 V. The triple mode changes in presence of Cu(II) suggesting the possible application of 1 for the detection of Cu(II) in aqueous media. Graphical Abstract.
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Using the close-aperture Z-scan technique, the pure nonlinear refractive index (n2) of carbon disulfide is measured with a 76 MHz repetition rate femtosecond laser. Strong interference of thermal effects exists with high-repetition-rate lasers that result in negative values of n2. We remove the thermal effect completely by continuously increasing the sample flow rate (F) in a sample cell as indicated by the change in sign of n2 from negative to positive. The positive value of n2 is due to Kerr-type nonlinearity. At sufficiently high values of F of >25 ml/min, all thermal effects are removed, resulting in an n2 value that matches low-repetition-rate experiments.
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In situ dynamics of colloidal cluster formation from nanoparticles is yet to be addressed. Using two-photon fluorescence (TPF) that has been amply used for single particle tracking, we demonstrate in situ measurement of effective three-dimensional optical trap stiffness of nanoparticles and their aggregates without using any position sensitive detector. Optical trap stiffness is an essential measure of the strength of an optical trap. TPF is a zero-background detection scheme and has excellent signal-to-noise-ratio, which can be easily extended to study the formation of colloidal cluster of nanospheres in the optical trapping regime. TPF tracking can successfully distinguish colloidal cluster from its monomer.
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We investigate the effect of cetyl-trimethyl-ammonium-bromides (CTAB) concentration on the fluorescence of Rhodamine-6G in water. This spectroscopic study of Rhodamine-6G in presence of CTAB was performed using two-photon-induced-fluorescence at 780 nm wavelength using high repetition rate femtosecond laser pulses. We report an increment of â¼10 % in the fluorescence in accordance with â¼12 % enhancement in the absorption intensity of the dye molecule around the critical micellar concentration. We discuss the possible mechanism for the enhancement in the two-photon fluorescence intensity and the importance of critical micellar concentration.
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We present a novel method of microrheology based on femtosecond optical tweezers, which in turn enables us to directly measure and control in situ temperature at microscale volumes at the solid-liquid interface. A noninvasive pulsed 780 nm trapped bead spontaneously responds to changes in its environment induced by a co-propagating 1560 nm pulsed laser due to mutual energy transfer between the solvent molecules and the trapped bead. Strong absorption of the hydroxyl group by the 1560 nm laser creates local heating in individual and binary mixtures of water and alcohols. "Hot Brownian motion" of the trapped polystyrene bead is reflected in the corner frequency deduced from the power spectrum. Changes in corner frequency values enable us to calculate the viscosity as well as temperature at the solid-liquid interface. We show that these experimental results can also be theoretically ratified.
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Stable optical trapping of dielectric nanoparticles with low power high-repetition-rate ultrafast pulsed excitation has received considerable attention in recent years. However, the exact role of such excitation has been quite illusive so far since, for dielectric micron-sized particles, the trapping efficiency turns out to be similar to that of continuous-wave excitation and independent of pulse chirping. In order to provide a coherent explanation of this apparently puzzling phenomenon, we justify the superior role of high-repetition-rate pulsed excitation in dielectric nanoparticle trapping which is otherwise not possible with continuous-wave excitation at a similar average power level. We quantitatively estimate the optimal combination of pulse peak power and pulse repetition rate leading to a stable trap and discuss the role of inertial response on the dependence of trapping efficiency on pulse width. In addition, we report gradual trapping of individual quantum dots detected by a stepwise rise in a two-photon fluorescence signal from the trapped quantum dots which conclusively proves individual particle trapping.
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We use a femtosecond pump-probe Z-scan technique to measure the thermal lens (TL) signal in a homologous series of primary alcohols. The trend in these experimentally measured TL signals deviates in a counterintuitive manner from the ones calculated using theoretical models that are only based on the macroscopic parameters. Introspection shows that the present TL theories are based on heat conduction for low absorbing samples without considering any convective mode of heat transfer. Our studies on highly absorbing samples indicate the importance of convective mode of heat transfer in TL studies.
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We explore the photothermal response of methanol, ethylene glycol, and glycerol using the femtosecond laser-induced thermal lens spectroscopy (FTLS) technique. A mode mismatched pump-probe spectroscopic technique was utilized to analyze the influence of localized thermal heating on the photothermal response of solvents. The findings revealed a strong dependence on both the input pump power and the molecular characteristics of the solvents. At significantly high pump power, the excess heat load deposited to the solvent is found to be responsible for the induction of the convection currents in the heat transfer mechanisms. Our results highlight that the influence of pump power on photothermal and thermal lens characteristics is intricately linked to the natural drifting and heat transfer mechanisms of solvent molecules. The molecular motion and existing connective processes were correlated with the molecular characteristics of the samples. The present finding reveals that FTLS is a sensitive probe for comprehending the impact of input laser power, molecular structure, and intermolecular H bonding on the photothermal characteristics and thermo-optical properties of the alcoholic medium.