The cropping system matters - Contrasting responses of winter faba bean (Vicia faba L.) genotypes to drought stress.

Intercropping of legumes and cereals provides many ecological advantages and contributes to a sustainable agriculture. These agricultural systems face ongoing shifts in precipitation patterns and seasonal drought. Although the effect of drought stress on legumes has been frequently studied, knowledge about water deficits influencing legumes under different cropping systems is still limited. Therefore, we investigated the impact of water deficit and re-irrigation on two winter faba bean genotypes (S_004 and S_062) and winter wheat (var. Genius) in pure and intercropped stands under greenhouse conditions. Various physiological and biochemical parameters, such as canopy surface temperature, leaf relative water content and proline content, were collected at three time points (beginning of water deficit, end of water deficit, after re-irrigation). In addition, water use efficiency (WUE) was analyzed at the end of the experiment. The overall drought stress tolerance was determined as conceptual analysis of all measured parameters. Water deficit significantly affected WUE, surface temperature and proline content of both winter faba bean genotypes. Interestingly, intercropping with wheat resulted in an overall high drought tolerance of genotype S_004, while genotype S_062 had a high drought tolerance in pure stands. Under water deficit, pure stands of S_062 substantially increased WUE by 30.5%. Intercropping of genotype S_004 increased the dry matter per plant by 31.7% compared to pure stands under water deficit. Contrary, intercropping of genotype S_062 did not improve the dry matter production. Our findings indicate that genotype S_004 benefits from resource complementarity in intercropping systems with wheat, whereas S_062 is better suitable for pure stands due to competitive effects. Thus, our study highlights that the drought tolerance of winter faba bean genotypes depends on the cropping system, leading to a demand for drought-adapted cultivars specifically selected for intercropping.

First Insights into the Draft Genome Sequence of the Endophyte Paenibacillus amylolyticus Strain GM1FR, Isolated from Festuca rubra L.

Paenibacillus amylolyticus strain GM1FR is an endophyte isolated from aerial plant tissues of Festuca rubra L. Here, we report the draft genome sequence (7.3 Mb) of GM1FR containing 6,241 protein-coding genes, some of which are potentially involved in plant growth promotion and biocontrol.

Draft Genome Sequence of the Endophyte Bacillus mycoides Strain GM5LP Isolated from Lolium perenne.

Bacillus mycoides GM5LP is a Gram-positive endophytic bacterium isolated from aerial plant tissues of Lolium perenne L. The 6.0-Mb draft genome harbors 6,132 protein-coding sequences, some of which might be involved in the biosynthesis of antimicrobial substances.

Draft Genome Sequence of Pseudomonas putida Strain GM4FR, an Endophytic Bacterium Isolated from Festuca rubra L.

Pseudomonas putida GM4FR is an endophytic bacterium isolated from aerial plant tissues of Festuca rubra L. Functional annotation of the draft genome (7.1 Mb) revealed 6,272 predicted protein-encoding genes. The genome provides insights into the biocontrol and plant growth-promoting potential of P. putida GM4FR.

The Effects of Cropping Regimes on Fungal and Bacterial Communities of Wheat and Faba Bean in a Greenhouse Pot Experiment Differ between Plant Species and Compartment.

Many bacteria and fungi in the plant rhizosphere and endosphere are beneficial to plant nutrient acquisition, health, and growth. Although playing essential roles in ecosystem functioning, our knowledge about the effects of multiple cropping regimes on the plant microbiome and their interactions is still limited. Here, we designed a pot experiment simulating different cropping regimes. For this purpose, wheat and faba bean plants were grown under controlled greenhouse conditions in monocultures and in two intercropping regimes: row and mixed intercropping. Bacterial and fungal communities in bulk and rhizosphere soils as well as in the roots and aerial plant parts were analyzed using large-scale metabarcoding. We detected differences in microbial richness and diversity between the cropping regimes. Generally, observed effects were attributed to differences between mixed and row intercropping or mixed intercropping and monoculture. Bacterial and fungal diversity were significantly higher in bulk soil samples of wheat and faba bean grown in mixed compared to row intercropping. Moreover, microbial communities varied between crop species and plant compartments resulting in different responses of these communities toward cropping regimes. Leaf endophytes were not affected by cropping regime but bacterial and fungal community structures in bulk and rhizosphere soil as well as fungal community structures in roots. We further recorded highly complex changes in microbial interactions. The number of negative inter-domain correlations between fungi and bacteria decreased in bulk and rhizosphere soil in intercropping regimes compared to monocultures due to beneficial effects. In addition, we observed plant species-dependent differences indicating that intra- and interspecific competition between plants had different effects on the plant species and thus on their associated microbial communities. To our knowledge, this is the first study investigating microbial communities in different plant compartments with respect to multiple cropping regimes using large-scale metabarcoding. Although a simple design simulating different cropping regimes was used, obtained results contribute to the understanding how cropping regimes affect bacterial and fungal communities and their interactions in different plant compartments. Nonetheless, we need field experiments to properly quantify observed effects in natural ecosystems.