Root-to-shoot signaling positively mediates source-sink relation in late growth stages in diploid and tetraploid wheat.

Non-hydraulic root source signaling (nHRS) is a unique positive response to soil drying in the regulation of plant growth and development. However, it is unclear how the nHRS mediates the tradeoff between source and sink at the late growth stages and its adaptive mechanisms in primitive wheat. To address this issue, a root-splitting design was made by inserting solid partition in the middle of the pot culture to induce the occurrence of nHRS using four wheat cultivars (MO1 and MO4, diploid; DM22 and DM31, tetraploid) as materials. Three water treatments were designed as 1) both halves watered (CK), 2) holistic root system watered then droughted (FS), 3) one-half of the root system watered and half droughted (PS). FS and PS were designed to compare the role of the full root system and split root system to induce nHRS. Leaves samples were collected during booting and anthesis to compare the role of nHRS at both growth stages. The data indicated that under PS treatment, ABA concentration was significantly higher than FS and CK, demonstrating the induction of nHRS in split root design and nHRS decreased cytokinin (ZR) levels, particularly in the PS treatment. Soluble sugar and proline accumulation were higher in the anthesis stage as compared to the booting stage. POD activity was higher at anthesis, while CAT was higher at the booting stage. Increased ABA (nHRS) correlated with source-sink relationships and metabolic rate (i.e., leaf) connecting other stress signals. Biomass density showed superior resource acquisition and utilization capabilities in both FS and PS treatment as compared to CK in all plants. Our findings indicate that nHRS-induced alterations in phytohormones and their effect on source-sink relations were allied with the growth stages in primitive wheat.

Transcriptome analysis of Acinetobacter calcoaceticus HX09 strain with outstanding crude-oil-degrading ability.

Microbial remediation plays a pivotal role in the elimination of petroleum pollutants, making it imperative to investigate the capabilities of microorganisms in degrading petroleum. The present study describes the isolation of a promising strain, Acinetobacter sp. HX09, from petroleum-contaminated water. GC-MS analysis revealed a remarkable removal efficiency for short and medium-chain alkanes, with a rate of approximately 64% after a 7-days incubation at 30 °C. Transcriptome analysis of HX09 exhibited a predominant upregulation in gene expression levels by the induce of crude oil. Notably, genes such as alkane 1-monooxygenase, dehydrogenases and fatty acid metabolic enzymes exhibited fold changes range from 3.16 to 1.3. Based on the alkB gene sequences in HX09, the Phyre2 algorithm generated a three-dimensional structure that exhibited similarity to segments of acyl coenzyme desaturases and acyl lipid desaturases. Furthermore, three biodegradation-related gene clusters were predicted in HX09 based on the reference genome sequence. These findings contribute to our understanding of the hydrocarbon-degrading mechanisms employed by Acinetobacter species and facilitate the development of effective remediation strategies for crude oil- polluted environments.

Response of source-sink relationship to progressive water deficit in the domestication of dryland wheat.

It is crucial to clarify the physiological responses of wheat (T. aestivum) plants to source-sink manipulation and assimilation transportation under drought stress during domestication of dryland wheat. In this research, a two-year field experiment was conducted using nine wheat cultivars in a semiarid site of northwest China. The source-sink manipulation treatments including defoliation of flag leaves and 50% removal of ears were applied at the anthesis stage under two levels of drought stress conditions i.e. progressive water supply (PWS) and rainfed drought treatment (RDT). Our results indicated that drought stress reduced the dry weight of leaves, sheaths and stems, as well as caused a significant yield reduction. High ploidy wheat exhibits a greater capacity to sustain higher grain yields when subjected to drought stress, primarily due to its stronger buffer capacity between source supply and sink demand. All wheat species with different ploidy levels had a certain degree of source limitation and sink restriction. During the domestication of wheat, the type of source and sink might be ploidy-dependent with progressive water deficit, but similar interactive relationships. The source-sink ratio of tetraploid species was the largest, while that of hexaploid species was the lowest.

Differentiate responses of tetraploid and hexaploid wheat (Triticum aestivum L.) to moderate and severe drought stress: a cue of wheat domestication.

Differentiate mechanism of wheat species in response to contrasting drought stress gradients implies a cue of its long-term domestication. In the present study, three water regimes including well-watered control (WW, 80% field water capacity (FC)), moderate drought stress (MS, 50% FC,) and severe drought stress (SS, 30% FC) were designed to reveal different responses of eight wheat species (four tetraploid and four hexaploid) representing different breeding decades and genetic origins to drought stresses. The data indicated that 50% FC and 30% FC fell into the soil moisture threshold range of non-hydraulic and hydraulic root signal occurrence, respectively. In general, grain yield, grain number/spike weight per plant, aboveground biomass, harvest index (HI) and water use efficiency (WUE) were significantly higher in hexaploid species than those of tetraploid species under drought stress (P < .05). Particularly, non-hydraulic root signal was triggered and continuously operated under 50% FC, while hydraulic root signal was observed under 30% FC, respectively. Under 80% FC, the allometric exponent (ɑ) of Maboveground vs Mroot decreased from tetraploid to hexaploid (both were of <1), indicating that during the domestication, the hexaploid species allocated less biomass to root system. For the relationship of Mear vs Mvegetative, the ɑ value was significantly greater in the hexaploid species, showing that hexaploid wheat distributed more biomass to ear than tetraploid to improve yield. Under 50% FC, this trend was enhanced. However, under 30% FC, there was no significant difference in the ɑ value between two species. Additionally, correlation analyses on yield formation affirmed the above results. Therefore, drought tolerance tended to be enhanced in hexaploid species under the pressure of artificial selection than that of tetraploid species. When drought stress exceeded a certain threshold, both species would be negatively seriously affected and followed a similar mechanism for better survival.