RESUMO
Celiac disease is a systemic autoimmune disease that affects 1.26% of the Argentine pediatric population. Our purpose was to study the prevalence of specific antibodies of celiac disease in a risk group and to find the association with specific factors. This was a cross-sectional study conducted in children ofa Nutritional Recovery Program from 1 year and 9 months to 6 years and 9 months old, from one Health Area of Tucumán Province, during 2010 and 2011, in a random cluster sample. It was studied a population of 175 children. We identified 3 cases with positive serology, equivalent to 1.7% of the study population. The association between cases with positive serology for celiac disease and other variables referred could not be established.
Assuntos
Autoanticorpos/sangue , Doença Celíaca/imunologia , Argentina , Criança , Pré-Escolar , Estudos Transversais , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imunodifusão , Imunoglobulina A/sangue , Lactente , Masculino , Transglutaminases/imunologiaRESUMO
We typed 1541 Y-STR haplotypes from reference samples along forensic casework investigations. In three haplotypes, we detected a variant allele designed as 16.3 at locus DYS533. This was confirmed by amplification using two commercial kits. Sanger sequencing revealing a novel motif corresponding to [TATC]12 repeats with a 19-bp insertion in the flanking upstream region. We propose its origin as an insertion at - 9.1 upstream of the repeat motifs. We searched other local databases and found this allele in various geographical areas of Argentina and neighbouring countries. The haplotypes share a common core of 10 Y-STRs (DYS389-I/13; DYS389-II/30; DYS19/14; DYS481/22; DYS438/12; DYS437/16; DYS635/23; DYS392/13; DYS393/13; GATA H4/11) and belong to the R1b haplogroup. This 16.3 allele is restricted to southern South America, which allows us to propose a local and relatively recent origin. The sequence described herein constitutes a novelty that could be considered in future criteria for the nomenclature of STRs based on massively parallel sequencing.