RESUMO
Although much effort has been extended to the efficient cancer therapies, the drug resistance is still a major obstacle in cancer chemotherapeutic treatments. Almost 90% of the cancer therapy failure is caused by the relative problems. Recently, the application of drug coated polymer nanospheres and nanoparticles to inhibit the related drug resistance has attracted much attention. In this report, we have explored a novel strategy to inhibit the multidrug resistance of the targeted tumor cells by combining the unique properties of tetraheptylammonium capped Fe(3)O(4) magnetic nanoparticles with the drug accumulation of anticancer drug daunorubicin. Our results of confocal fluorescence and atomic force microscopy (AFM) as well as electrochemical studies demonstrate the remarkable synergistic effect of Fe(3)O(4) nanoparticles on drug uptake of daunorubicin in leukemia K562 cells. These observations indicate that the interaction between the magnetic nanoparticles Fe(3)O(4) and biologically active molecules on the membrane of leukemia cell lines may contribute to their beneficial effect on cellular uptake so that the synergistic enhanced effect of magnetic nanoparticles Fe(3)O(4) on drug uptake of drug resistance leukemia K562 cells could be observed upon application of the Fe(3)O(4) nanoparticles.
Assuntos
Antibióticos Antineoplásicos/farmacologia , Daunorrubicina/farmacologia , Resistência a Múltiplos Medicamentos , Resistencia a Medicamentos Antineoplásicos , Compostos Férricos , Leucemia/tratamento farmacológico , Nanopartículas , Antibióticos Antineoplásicos/uso terapêutico , Materiais Revestidos Biocompatíveis , Daunorrubicina/uso terapêutico , Sistemas de Liberação de Medicamentos , Resistência a Múltiplos Medicamentos/efeitos dos fármacos , Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos , Humanos , Células K562 , Leucemia/patologia , Magnetismo , Compostos de Amônio QuaternárioRESUMO
Fe3O4 nanoparticles are the most commonly used magnetic materials with promising applications in biomedical and biochemical engineering. In this study, a novel application of the tetraheptylammonium capped Fe3O4 nanoparticles in controllable biorecognition process of anticancer drug doxorubicin through combination with external static magnetic field has been demonstrated. Our AFM and electrochemical studies illustrate that the presence of the tetraheptylammonium capped Fe3O4 nanoparticles could promote the binding behavior of doxorubicin to DNA. And the results of the electrochemical contact angle measurements indicate that the controllable biomolecular recognition of doxorubicin could be readily achieved by combining these functionalized Fe3O4 nanoparticles with changing the positions of external magnetic field.
Assuntos
Antibióticos Antineoplásicos/química , Doxorrubicina/química , Compostos Férricos/química , Magnetismo , Nanopartículas Metálicas/química , Nanocompostos/química , Engenharia Biomédica , Carbono/química , DNA/química , Eletroquímica/métodos , Microscopia de Força Atômica , Microscopia Eletrônica de Transmissão , Modelos Químicos , Compostos de Amônio Quaternário/químicaRESUMO
The interaction between procaine hydrochloride and DNA/DNA bases in the absence and presence of cadmium sulfide (CdS) nanoparticles has been explored in this study by using differential pulse voltammetry, atomic force microscopy (AFM) and so on, which illustrates the different binding behaviors of procaine hydrochloride with different DNA bases. The results clearly indicate that the binding of purines to procaine hydrochloride is stronger than that of pyrimidines and the binding affinity is in the order of G > A > T > C. In addition, it was observed that the presence of CdS nanoparticles could remarkably enhance the probing sensitivity for the interaction between procaine hydrochloride and DNA/DNA bases. Furthermore, AFM study illustrates that procaine hydrochloride can bind to some specific sites of DNA chains, which indicates that procaine hydrochloride may interact with some special sequences of DNA.
Assuntos
Compostos de Cádmio/farmacologia , DNA/química , Procaína/química , Purinas/química , Pirimidinas/química , Sulfatos/farmacologia , Sítios de Ligação , DNA/efeitos dos fármacos , DNA/ultraestrutura , Microscopia de Força Atômica , Modelos Moleculares , Nanoestruturas/químicaRESUMO
A one pot method for organic/colloidal CdSe nanoparticle hybrid material synthesis is presented. Relative to traditional ligand exchange processes, these materials require smaller amounts of the desired capping ligand, shorter syntheses and fewer processing steps, while maintaining nanoparticle morphology.