Wintertime pharmacokinetics of intravenously and orally administered meloxicam in semi-domesticated reindeer (Rangifer tarandus tarandus).

OBJECTIVE: To investigate the pharmacokinetics of orally and intravenously (IV) administered meloxicam in semi-domesticated reindeer (Rangifer tarandus tarandus). STUDY DESIGN: A crossover design with an 11 day washout period. ANIMALS: A total of eight young male reindeer, aged 1.5-2.5 years and weighing 74.3 ± 6.3 kg, mean ± standard deviation. METHODS: The reindeer were administered meloxicam (0.5 mg kg-1 IV or orally). Blood samples were repeatedly collected from the jugular vein for up to 72 hours post administration. Plasma samples were analysed for meloxicam concentrations with ultraperformance liquid chromatography combined with triple quadrupole mass spectrometry. Noncompartmental analysis for determination of pharmacokinetic variables was performed. RESULTS: The pharmacokinetic values, median (range), were determined. Elimination half-life (t½) with the IV route (n = 4) was 15.2 (13.2-16.8) hours, the volume of distribution at steady state was 133 (113-151) mL kg-1 and clearance was 3.98 (2.63-5.29) mL hour-1 kg-1. After oral administration (n = 7), the peak plasma concentration (Cmax) was detected at 6 hours, t½ was 19.3 (16.7-20.5) hours, Cmax 1.82 (1.17-2.78) µg mL-1 and bioavailability (n = 3) 49 (46-73)%. No evident adverse effects were detected after either administration route. CONCLUSIONS AND CLINICAL RELEVANCE: A single dose of meloxicam (0.5 mg kg-1 IV or orally) has the potential to maintain the therapeutic concentration determined in other species for up to 3 days in reindeer plasma.

Invited review: Lying time and the welfare of dairy cows.

Adequate time lying down is often considered an important aspect of dairy cow welfare. We examine what is known about cows' motivation to lie down and the consequences for health and other indicators of biological function when this behavior is thwarted. We review the environmental and animal-based factors that affect lying time in the context of animal welfare. Our objective is to review the research into the time that dairy cows spend lying down and to critically examine the evidence for the link with animal welfare. Cows can be highly motivated to lie down. They show rebound lying behavior after periods of forced standing and will sacrifice other activities, such as feeding, to lie down for an adequate amount of time. They will work, by pushing levers or weighted gates, to lie down and show possible indicators of frustration when lying behavior is thwarted. Some evidence suggests that risk of lameness is increased in environments that provide unfavorable conditions for cows to lie down and where cows are forced to stand. Lameness itself can result in longer lying times, whereas mastitis reduces it. Cow-based factors such as reproductive status, age, and milk production influence lying time, but the welfare implications of these differences are unknown. Lower lying times are reported in pasture-based systems, dry lots, and bedded packs (9 h/d) compared with tiestalls and freestalls (10 to 12 h/d) in cross-farm research. Unfavorable conditions, including too few lying stalls for the number of cows, hard or wet lying surfaces, inadequate bedding, stalls that are too small or poorly designed, heat, and rain all reduce lying time. Time constraints, such as feeding or milking, can influence lying time. However, more information is needed about the implications of mediating factors such as the effect of the standing surface (concrete, pasture, or other surfaces) and cow behavior while standing (e.g., being restrained, walking, grazing) to understand the effect of low lying times on animal welfare. Many factors contribute to the difficulty of finding a valid threshold for daily lying time to use in the assessment of animal welfare. Although higher lying times often correspond with cow comfort, and lower lying times are seen in unfavorable conditions, exceptions occur, namely when cows lie down for longer because of disease or when they spend more time standing because of estrus or parturition, or to engage in other behaviors. In conclusion, lying behavior is important to dairy cattle, but caution and a full understanding of the context and the character of the animals in question is needed before drawing firm conclusions about animal welfare from measures of lying time.

A comparison of sedative effects of xylazine alone or combined with levomethadone or ketamine in calves prior to disbudding.

OBJECTIVE: To compare the sedative effects of intramuscular xylazine alone or combined with levomethadone or ketamine in calves before cautery disbudding. STUDY DESIGN: Randomized, blinded, clinical trial. ANIMALS: A total of 28 dairy calves, aged 21 ± 5 days and weighing 61.0 ± 9.3 kg (mean ± standard deviation). METHODS: Calves were randomly allocated to three groups: xylazine (0.1 mg kg-1) and levomethadone (0.05 mg kg-1; group XL), xylazine (0.1 mg kg-1) and ketamine (1 mg kg-1; group XK) and xylazine alone (0.2 mg kg-1; group X). Local anaesthesia (procaine hydrochloride) and meloxicam were administered subcutaneously 15 minutes after sedation and 15 minutes before disbudding. The calves' responses to the administration of local anaesthesia and disbudding were recorded. Sedation was assessed at baseline and at intervals up to 240 minutes postsedation. Times of recumbency, first head lift and first standing were recorded. Drug plasma concentrations were measured. RESULTS: Data were obtained from 27 animals. All protocols resulted in sedation sufficient to administer local anaesthesia and to perform disbudding. Sedation scores significantly correlated with drug plasma concentrations (p ≤ 0.002). Times to recumbency did not differ among protocols (2.8 ± 0.3, 3.1 ± 1.1 and 2.1 ± 0.8 minutes for groups XL, XK and X, respectively), whereas interval from drug(s) administration until first head lift was significantly shorter in group XK than X (47.3 ± 14.1, 34.4 ± 5.3 and 62.6 ± 31.9 minutes for groups XL, XK and X, respectively). The area under the time-sedation curve was significantly greater in group X than XK or XL (754 ± 215, 665 ± 118 and 1005 ± 258 minutes for groups XL, XK and X, respectively). CONCLUSIONS AND CLINICAL RELEVANCE: Levomethadone or ketamine with a low dose of xylazine produced short but sufficient sedation for local anaesthesia and disbudding with minimum resistance.

The First Rehoming of Laboratory Beagles in Finland: The Complete Process from Socialisation Training to Follow-up.

The fate of experimental animals represents an ethical dilemma and a public concern. In the EU, Directive 2010/63/EU allows the rehoming of former experimental animals instead of euthanasia. However, to our knowledge, there are no previous reports of rehoming Beagles in Finland. This study aimed to describe the process behind the first rehoming of laboratory Beagles at the University of Helsinki and evaluate its success. In total, 16 former laboratory Beagles were rehomed in collaboration with animal protection organisations and the University of Helsinki. The dogs had participated in animal cognition studies and had undergone minor procedures during the development of a veterinary drug. While the dogs were still in the laboratory, a socialisation training programme lasting several months was undertaken. Through surveying of the adoptive owners, and interviewing the various stakeholders involved (researchers, animal protection organisations and animal caretakers), the overall process was evaluated, including: the socialisation training programme; the comparative success of rehoming younger compared to older animals; the criteria that were used for the selection of the adoptive owners; and the eventual success of rehoming the dogs with the new owners. The majority of the dogs adjusted well to their new home environment. Euthanasia at the end of their experimental use would have been unnecessary and possibly against the objectives of European directives.

Rapid eye movement sleep time in dairy cows changes during the lactation cycle.

The importance of rest and sleep is well established; we know, for example, that lack of sleep impairs immune function in rats and increases pain sensitivity in humans. However, little is known about sleep in dairy cows, but a lack of rest and sleep is discussed as a possible welfare problem in cattle. A first step toward a better understanding of sleep in dairy cows is to quantify the time cows spend awake and asleep in different stages of lactation. Using electrophysiological recordings on 7 occasions in wk -2, 2, 7, 13, 22, 37, and 45 in relation to calving, we investigated changes in rapid eye movement (REM) sleep time as well as non-rapid eye movement (NREM) sleep, drowsing, awake, and rumination in 19 dairy cows of the Swedish Red breed kept in single pens with ad libitum access to feed and water. The recordings on wk -2 and 45 were conducted during the dry period, and all others during lactation. The PROC MIXED procedure in SAS (SAS Institute Inc., Cary, NC) was used to test for significant differences in REM, NREM, drowsing, awake, and rumination between the different stages of lactation cycle. Pairwise comparisons between all recording occasions showed that total REM sleep duration was shorter for cows in wk 2 relative to calving compared with wk -2, and the number of REM sleep bouts were fewer in wk 2 compared with wk -2. The REM sleep was recorded during both the day (0500-2100 h) and night (2100-0500 h), but predominantly performed at night compared with daytime, and the bout duration was longer during nighttime compared with daytime. A tendency was observed for time spent in NREM sleep to be shorter in wk 2 relative to calving compared with wk -2. The duration spent drowsing was shorter for cows in wk 2 and 13 relative to calving compared with wk -2. We found no effect of stage of lactation cycle on the duration of awake or ruminating. Our study is the first to assess sleep distribution during a lactation cycle, and our results show that stage of lactation is important to consider when moving forward with sleep investigations in dairy cows. The shortest REM sleep duration was found for cows 2 wk after calving and longest 2 wk before calving, and the difference was due a higher number of REM sleep bouts in the recording 2 wk before calving. The REM sleep and rumination predominantly occurred at night but were recorded during both day and night.

Teaching Tip-Studying to Become a Veterinarian: A Course for Student Support.

During the last decade, concerns over veterinary students' stress have been expressed in several studies, and the need for student support has become evident. In addition, the importance of professional and personal identity development in veterinary curricula has been widely recognized. There is a need to integrate academic and professional skills instruction with training in personal-life balance. Even though tools for student support and stress management exist within universities, reports on active and creative practices in veterinary education are scarce. We report here a course that has been organized twice as an optional part of veterinary studies to provide students with tools for everyday life and personal development toward a future veterinary career. Students defined their own learning objectives in this course, and they reported having received tools and knowledge especially for time management and stress control. The course gave the students an opportunity to step back from their busy schedules, think over their lives and actions, and even take concrete actions that have a positive effect on their well-being. The rich qualitative material collected during the pilot course has been used not only for developing the course further but also for development of the mandatory curriculum.

How dogs scan familiar and inverted faces: an eye movement study.

Faces play an important role in communication and identity recognition in social animals. Domestic dogs often respond to human facial cues, but their face processing is weakly understood. In this study, facial inversion effect (deficits in face processing when the image is turned upside down) and responses to personal familiarity were tested using eye movement tracking. A total of 23 pet dogs and eight kennel dogs were compared to establish the effects of life experiences on their scanning behavior. All dogs preferred conspecific faces and showed great interest in the eye area, suggesting that they perceived images representing faces. Dogs fixated at the upright faces as long as the inverted faces, but the eye area of upright faces gathered longer total duration and greater relative fixation duration than the eye area of inverted stimuli, regardless of the species (dog or human) shown in the image. Personally, familiar faces and eyes attracted more fixations than the strange ones, suggesting that dogs are likely to recognize conspecific and human faces in photographs. The results imply that face scanning in dogs is guided not only by the physical properties of images, but also by semantic factors. In conclusion, in a free-viewing task, dogs seem to target their fixations at naturally salient and familiar items. Facial images were generally more attractive for pet dogs than kennel dogs, but living environment did not affect conspecific preference or inversion and familiarity responses, suggesting that the basic mechanisms of face processing in dogs could be hardwired or might develop under limited exposure.

Sublingual administration of detomidine to calves prior to disbudding: a comparison with the intravenous route.

OBJECTIVE: To study the effects of oromucosal detomidine gel administered sublingually to calves prior to disbudding, and to compare its efficacy with intravenously (IV) administered detomidine. STUDY DESIGN: Randomised, prospective clinical study. ANIMALS: Twenty dairy calves aged 12.4 ± 4.4days (mean ± SD), weight 50.5 ± 9.0 kg. METHODS: Detomidine at 80 µg kg(-1) was administered to ten calves sublingually (GEL) and at 30 µg kg(-1) to ten control calves IV (V. jugularis). Meloxicam (0.5 mg kg(-1) ) and local anaesthetic (lidocaine 3 mg kg(-1) ) were administered before heat cauterization of horn buds. Heart rate (HR), body temperature and clinical sedation were monitored over 240 minutes. Blood was collected from the V. cephalica during the same period for drug concentration analysis. Pharmacokinetic variables were calculated from the plasma detomidine concentration-time data using non-compartmental methods. Statistical analyses compared routes of administration by Student's t-test and linear mixed models as relevant. RESULTS: The maximum plasma detomidine concentration after GEL was 2.1 ± 1.2 ng mL(-1) (mean ±SD) and the time of maximum concentration was 66.0 ± 36.9 minutes. The bioavailability of detomidine was approximately 34% with GEL. Similar sedation scores were reached in both groups after administration of detomidine, but maximal sedation was reached earlier in the IV group (10 minutes) than in the GEL group (40 minutes). HR was lower after IV than GEL from 5 to 10 minutes after administration. All animals were adequately sedated, and we were able to administer local anaesthetic without resistance to all of the calves before disbudding. CONCLUSIONS AND CLINICAL RELEVANCE: Oromucosally administered detomidine is an effective sedative agent for calves prior to disbudding.

Visual event-related potentials of dogs: a non-invasive electroencephalography study.

Previously, social and cognitive abilities of dogs have been studied within behavioral experiments, but the neural processing underlying the cognitive events remains to be clarified. Here, we employed completely non-invasive scalp-electroencephalography in studying the neural correlates of the visual cognition of dogs. We measured visual event-related potentials (ERPs) of eight dogs while they observed images of dog and human faces presented on a computer screen. The dogs were trained to lie still with positive operant conditioning, and they were neither mechanically restrained nor sedated during the measurements. The ERPs corresponding to early visual processing of dogs were detectable at 75-100 ms from the stimulus onset in individual dogs, and the group-level data of the 8 dogs differed significantly from zero bilaterally at around 75 ms at the most posterior sensors. Additionally, we detected differences between the responses to human and dog faces in the posterior sensors at 75-100 ms and in the anterior sensors at 350-400 ms. To our knowledge, this is the first illustration of completely non-invasively measured visual brain responses both in individual dogs and within a group-level study, using ecologically valid visual stimuli. The results of the present study validate the feasibility of non-invasive ERP measurements in studies with dogs, and the study is expected to pave the way for further neurocognitive studies in dogs.

Euthanasia and the rehabilitation of wildlife casualties in Finland: decision-making varies depending on the background education of the caregivers.

Caring for wildlife casualties is a common aspect of animal-protection work. The range of care options of wildlife in Finland vary from professional zoos to voluntary members of the public. There are complex ethical concerns to be considered in deciding whether an injured animal should be treated or euthanized. Differing opinions and poor communication may lead to unnecessary conflicts among caregivers. We investigated opinions behind the decision-making of caregivers related to wildlife casualties using a web-based questionnaire. We asked the respondents to rate their level of agreement with 27 statements on a seven-point Likert scale. Seventy-eight respondents were included in our analysis. Animal-related education was classified as veterinarian (n = 14), other (n = 18), and none (n = 49). The median (IQR) levels of age and work experience were 43 (17) and 5 (9) years, respectively, regardless of educational level. The groups were tested for differences level of agreement with the statements in Kruskall-Wallis tests (with Bonferroni-corrected pair-wise tests). Overall, the strongest disagreement was with statements proposing euthanasia on the grounds that the species was common [1 (2)], the treatment would be costly [1 (4)] or long-term [1 (4)], or there was no end-of-life-solution immediately available [1 (2)]. The highest agreement was with the statement advocating not euthanizing the animal if it could easily be returned to its natural habitat [7 (0)]. The respondents differed in their perceptions depending on their animal-related education. The cost and length of treatment, the prevalence of the species, and a known end-of-life solution influenced the euthanasia-related decisions of veterinarians more than of respondents in the other educational groups. Those with no animal-related education expressed the least willingness to euthanize an injured wild animal, even if it would be partly dependent on humans for the rest of its life or even if the treatment would be very stressful. We concluded that attitudes and practices related to euthanasia differ depending on the respondents' education, and that more discussion is needed on the ethical aspects behind the decision-making. This would help to increase mutual understanding among caregivers and facilitate the development of uniform standards that would potentially benefit animal welfare.

Histopathological findings in a pilot study of dairy calves disbudded with hot cauterization or caustic paste.

We describe the histological tissue damage and compare the healing process in 16 dairy calves disbudded at a mean age of 6 days by cauterization or alkaline caustic paste application. Biopsies were taken 2 days (T2) and 2 weeks (T14) after disbudding from sedated calves treated with local anaesthetic and non-steroidal anti-inflammatory drugs. At T2, the cauterized horn buds generally had eosinophilic coagulative necrosis of the epidermis and superficial dermis, bordered basally by a neutrophilic demarcation zone. Lateral to the direct heat contact area, dermal blood vessels were thrombosed, with wall damage and perivascular neutrophils. In the caustic paste-treated horn buds, the epidermis and dermis had diffuse full-thickness liquefactive necrosis directly under the paste contact area. The necrosis spread laterally in the dermis beyond the area of paste contact and was bordered by a neutrophilic infiltrate. At T14, the cauterized horn buds had epidermal to superficial dermal ulceration and crusting, dermal neutrophilic infiltration and granulation tissue formation. In contrast, most of the caustic paste-treated horn buds consisted of a superficial dermal crust or predominantly necrotic tissue fragments. The remaining viable areas had histiocytic inflammation with peripheral neutrophils and early granulation tissue formation. Caustic paste disbudding caused poorly demarcated lesions that were more severe and extensive and took longer to heal than those due to cautery. Cauterization induced a more intense acute reaction adjacent to the primary lesion compared with caustic paste.

Dogs do look at images: eye tracking in canine cognition research.

Despite intense research on the visual communication of domestic dogs, their cognitive capacities have not yet been explored by eye tracking. The aim of the current study was to expand knowledge on the visual cognition of dogs using contact-free eye movement tracking under conditions where social cueing and associative learning were ruled out. We examined whether dogs spontaneously look at actual objects within pictures and can differentiate between pictures according to their novelty or categorical information content. Eye movements of six domestic dogs were tracked during presentation of digital color images of human faces, dog faces, toys, and alphabetic characters. We found that dogs focused their attention on the informative regions of the images without any task-specific pre-training and their gazing behavior depended on the image category. Dogs preferred the facial images of conspecifics over other categories and fixated on a familiar image longer than on novel stimuli regardless of the category. Dogs' attraction to conspecifics over human faces and inanimate objects might reflect their natural interest, but further studies are needed to establish whether dogs possess picture object recognition. Contact-free eye movement tracking is a promising method for the broader exploration of processes underlying special socio-cognitive skills in dogs previously found in behavioral studies.

Animal Welfare Control-Inspection Findings and the Threshold for Requesting a Police Investigation.

An increasing proportion of animal welfare violations in Finland are related to companion animals. However, only a small number of these issues are investigated or prosecuted. The aims of this study were (i) to describe the inspection findings and the resulting actions of the official municipal veterinarians in the Finnish Capital Region and (ii) to identify the factors that predict their submissions of investigation requests to the police. Our data consisted of animal welfare complaints and official veterinarians' inspection reports and decisions from 811 cases of animal welfare control in the Finnish Capital Region. The data covered the period from March 2019 to April 2020. We performed logistic regression analyses to identify the factors that best predict when official veterinarians detect non-compliances and report the cases for police investigation. In 86% (696/811) of the cases, the veterinarians performed at least one animal welfare inspection, and/or received information from the police, or otherwise investigated the complaint. The most common forms of non-compliance were lack of basic maintenance and care (42%, 295/696) and insufficient veterinary care (27%, 185/696). The veterinarians requested for a police investigation in 9.6% (44/460) of all cases with detected non-compliances. The best predictors for detecting non-compliances with the animal welfare legislation were complaints of insufficient veterinary care (OR 1.9, CI 1.1-3.4), the cases assessed by the information from the police and/or an animal shelter (OR 15.2, CI 7.9-29.2), at least one inspection in an animal's premises with prior warning (OR 11.2, CI 5.5-22.6), and without prior warning (OR 17.0, CI 9.7-29.5). Complaints of violence against animals were negatively associated with detecting non-compliances (OR 0.5, CI 0.3-0.9). However, the detection of violence against animals predicted requests for police investigations (OR 9.3, CI 3.1-27.9), as did the execution of permanent urgent measures by official veterinarians (OR 4.9, CI 1.9-12.9). To improve the animal welfare control system and the investigation of crimes against animals, cooperation between officials should be developed. Further studies are needed to improve the understanding of the prevalence of violence against animals, and to advance methods used by animal welfare control to identify cases of violence.

Evaluation of Criminal Sanctions Concerning Violations of Cattle and Pig Welfare.

EU legislation requires the violations of animal welfare standards to be sanctioned. Our aim was to evaluate criminal sanctions concerning violations of cattle and pig welfare on Finnish farms. We analyzed 196 court cases heard in Finnish district courts from 2011 to 2016. Almost all the cases (95%) concerned the violations of cattle welfare, of which 61% occurred on small farms. The lack of cleanliness and inadequate feeding and watering were the most common reported violations. Median time span from the start date of an offending to a judgement was slightly less than two years. Of the cases, 96% resulted in conviction. The court did not perceive the violations as being highly blameworthy as a small fine and a short conditional imprisonment were the most often imposed sanctions. A ban on the keeping of animals was used as a precautionary measure in half of the cases. Veterinarians were shown to have an important role in the initiation of criminal procedures, providing evidence for the police, and acting as witnesses. Therefore, it is crucial to achieve a well-functioning collaboration between veterinarians and the police and prosecutors. The expertise of these authorities on animal welfare legislation should also be emphasized to improve the efficacy of criminal procedures.

Veterinarians Experience Animal Welfare Control Work as Stressful.

The aim of the study was to evaluate the job satisfaction of official veterinarians working in the field of animal welfare control and identify both positive features and challenges of their work. An electronic questionnaire was designed to evaluate job satisfaction. The questionnaire was responded to by 73 of the 98 Finnish official veterinarians working in the field of animal welfare control. The Spearman's rank correlation coefficient was used to evaluate the relation between stress and different work-related factors. More than half of the respondents reported work-related stress or fatigue. Threatening situations, disturbed work-private life balance and a high amount of overtime work were found to be frequent underlying causes of stress. Fieldwork, especially when working alone, was perceived as the most challenging part of the work. Of the respondents, three out of four performed animal welfare inspections mainly alone. Although the respondents reported getting additional help to perform an inspection most of the times they needed it, a wish to work in a pair was highlighted. The results of the present study indicate that official veterinarians often experience work-related stress and fatigue. By testing interventions shown to be beneficial, such as providing adequate support within the work community, decreasing the workload and enabling inspections to be done in pairs, job satisfaction could be improved.

Assessment of Welfare Problems in Finnish Cattle and Pig Farms Based on Official Inspection Reports.

The competent authorities of the Member States of the European Union are required to perform animal welfare inspections on livestock farms. The data obtained from these official inspections performed in Finnish cattle and pig farms in 2010-2015 were used with the aim of estimating the prevalence of the most common non-compliances and identifying underlying risk factors. The prevalence of non-compliant cattle and pig farms was 24.2% and 27.9%, respectively. In cattle, the most common problem was an inadequate lying area followed by deficient housing conditions for calves; in pigs, it was a lack of enrichment material. The non-compliances concerning cattle were most frequently detected in autumn and in farms with small herd size, with tie-stall housing and outdoor rearing year-round. The pig farms with a farrow-to-finish unit had a higher prevalence of non-compliances than other production types. The prevalence of the non-compliant farms differed notably between the regions. It can be concluded that the cattle welfare inspections should be performed with a focus on the cold and rainy seasons and at small farms, whereas the pig welfare inspections should mainly focus on farrow-to-finish units. The data received from official inspections should be efficiently utilized in the development of animal welfare inspection system, with the aim of risk-based, consistent and uniform inspections. In addition, the data should be utilized in targeting information for farmers.

Salivary IgG levels in neonatal calves and its association to serum IgG: an observational pilot study.

The diagnosis of inadequate transfer of colostrum immunoglobulin G (IgG) to calf serum, often known as failure of passive transfer (<10 g/L IgG1 at 24 to 48 h), necessitates blood sampling from the calf and in some instances the presence of a veterinarian. Sampling saliva is both less invasive and easy for the producer. Previous research has shown that quantification of saliva IgG is possible in juvenile and adult cattle. The objectives of this observational pilot study were to investigate whether IgG can be quantified in neonatal calf saliva, if it is correlated to serum IgG concentrations, and if the indirect quantification of saliva IgG is achievable by use of a digital refractometer. Paired blood and saliva samples were collected from 20 healthy dairy calves aged 1 to 3 d. In these samples, IgG was quantified directly with single radial immunodiffusion and indirectly by use of a digital refractometer indicating Brix % (a subsample of n = 12 saliva samples). A strong positive correlation (r = 0.7, P < 0.001) between saliva IgG (mean ± SD; 0.2 ± 0.11 g/L) and serum IgG (32.1 ± 11.94 g/L) was found. Saliva IgG ranged from the lowest detectable value, 0.1 g/L (n = 6 samples) to 0.6 g/L. Saliva Brix (1.2 ± 0.69%) was not significantly correlated to serum IgG (n = 12, r = 0.43, P = 0.155); however, it was significantly correlated to saliva IgG (n = 12, r = 0.7, P = 0.018) and Brix in serum (n = 12, r = 0.7, P = 0.013). We conclude that IgG was quantifiable in most of the saliva samples. For saliva IgG to be of any value with regards to detecting failure of passive transfer, future studies should investigate methods that can detect IgG <0.1 g/L. The results indicate that saliva IgG can be used to predict serum IgG at levels above 10 g/L, which may warrant further exploration of the use of saliva in the surveillance of failure of passive transfer. The results of the current pilot study did not support the potential usage of a Brix % refractometer to quantify saliva IgG.

Animal Ethical Views and Perception of Animal Pain in Veterinary Students.

Veterinary students face several ethical challenges during their curriculum. We used the Animal Ethics Dilemma to study animal ethical views of Finnish veterinary students, and also asked them to score the level of pain perception in 13 different species. Based on the 218 respondents, the utilitarian view was the dominating ethical view. Mammals were given higher pain scores than other animals. The proportion of the respect for nature view correlated negatively, and that of the animal rights view positively, with most animal pain scores. Fifth year students had a higher percentage of contractarian views, as compared to 1st and 3rd year students, but this might have been confounded by their age. Several pain perception scores increased with increasing study years. We conclude that the utilitarian view was clearly dominating, and that ethical views differed only slightly between students at different stages of their studies. Higher pain perception scores in students at a later stage of their studies might reflect an increased knowledge of animal capacities.

First-night effect on sleep time in dairy cows.

In human sleep studies, the probability of discomfort from the electrodes and the change in environment usually results in first-night recordings being discarded. Sleep recordings from the first night in human subjects often differ in amount of REM (rapid eye movement) sleep and the overall sleep architecture. This study investigated whether recordings of sleep states in dairy cows also show a first-night effect. Non-invasive electrophysiological recordings were carried out on nine cows of the Swedish Red breed during three consecutive 24-hour periods (recording days 1-3). Overall, cows spent 12.9 ± 1.4 hours awake, 8.2 ± 1 hours ruminating, 57.2 ± 20.3 min drowsing, 44.1 ± 20.2 min in REM sleep and 64.3 ± 38.1 min in NREM (non-rapid eye movement) sleep (mean ± SD) and there were no significant differences between recording days in total duration for any of the sleep and awake states. However, the bouts of REM sleep and rumination were longer, and the awake bouts were shorter, at night time compared to daytime, regardless of recording day. The awake bouts also showed an interaction effect with longer bouts at daytime during day 1 compared to daytime on day 3. Data on sleep and awake states recorded in adult dairy cows during three consecutive 24-h periods showed great variation in sleep time between cows, but total time for each state was not significantly affected by recording day. Further and more detailed studies of how sleep architecture is affected by recording day is necessary to fully comprehend the first-night effect in dairy cows.