Melatonin duration gates photoperiodic vocal state change in a songbird.

Seasonally breeding animals concentrate courtship to a particular time of year such that their offspring will be reared in a favorable environment. In house sparrows, Passer domesticus, primary (gonads) and secondary (song, plumage, beak color, etc) sexual characteristics are expressed differentially depending on the photoperiod. Removal of the pineal gland (PINX) has no effect on seasonal rhythms in gonad size but alters the photostimulated increase in vocal rate and complexity. Administration of long durations of melatonin, indicative of short days of winter, prevents seasonal recrudescence of song control nuclei in photostimulated house sparrows. In this study, male PINX house sparrows were exposed to three durations of melatonin, while vocalization and locomotor behavior were recorded as they were transitioned from short photoperiod to equinoctial photoperiods of spring. Birds receiving short duration melatonin or vehicle control increased dawn and dusk choruses as well as call complexity. Long durations of melatonin prevented this expansion to a spring-like vocal state observed in birds receiving the short duration of melatonin or vehicle control. The daily distribution of locomotor activity, beak color, and testis size was unaffected by treatment. Vocal state change was defined by our measures in two capacities: (i) increased dawn and dusk choruses, and (ii) an increase in calls associated with territory and mate attraction compared to the winter-like "social song." We conclude that house sparrows use the calendar information provided by melatonin duration to control seasonal vocalization behavior, independent of effects on and of the gonads.

The role of the pineal gland in the photoperiodic control of bird song frequency and repertoire in the house sparrow, Passer domesticus.

Temperate zone birds are highly seasonal in many aspects of their physiology. In mammals, but not in birds, the pineal gland is an important component regulating seasonal patterns of primary gonadal functions. Pineal melatonin in birds instead affects seasonal changes in brain song control structures, suggesting the pineal gland regulates seasonal song behavior. The present study tests the hypothesis that the pineal gland transduces photoperiodic information to the control of seasonal song behavior to synchronize this important behavior to the appropriate phenology. House sparrows, Passer domesticus, expressed a rich array of vocalizations ranging from calls to multisyllabic songs and motifs of songs that varied under a regimen of different photoperiodic conditions that were simulated at different times of year. Control (SHAM) birds exhibited increases in song behavior when they were experimentally transferred from short days, simulating winter, to equinoctial and long days, simulating summer, and decreased vocalization when they were transferred back to short days. When maintained in long days for longer periods, the birds became reproductively photorefractory as measured by the yellowing of the birds' bills; however, song behavior persisted in the SHAM birds, suggesting a dissociation of reproduction from the song functions. Pinealectomized (PINX) birds expressed larger, more rapid increases in daily vocal rate and song repertoire size than did the SHAM birds during the long summer days. These increases gradually declined upon the extension of the long days and did not respond to the transfer to short days as was observed in the SHAM birds, suggesting that the pineal gland conveys photoperiodic information to the vocal control system, which in turn regulates song behavior.

Context and novelty increase strength of auditory cues as a weak circadian zeitgeber in songbirds.

Light is the best-studied external cue (zeitgeber) for the entrainment of circadian rhythms. Non-photic entrainment is also possible; some organisms can entrain to rhythmic temperatures, drug administration, feeding, water turbulence, exercise and social cues. One such social cue that has the capacity to act as a weak zeitgeber to songbirds is the rhythmic presentation of conspecific vocalization. To better characterize this phenomenon, we performed several trials in which male and female zebra finches were maintained in constant dim light and allowed to free-run for 1 week before being presented with different audio cues of various lengths of playback and audio design every day at the same time of day for 15-31 days. Live audio monitoring from a nearby colony housed in light: dark (LD) conditions proved the strongest zeitgeber we tested, suggesting the phenomenon is enhanced with dynamic, context-appropriate vocalizations. Live colony playback was more efficacious than was a 2 h or 4 h presentation of the same, single zebra finch song but not a 1 h presentation, suggesting that habituation may have occurred in some of these experiments. The monitoring of the colony was also not statistically different from a 4 h playback of that same song, reversed, suggesting that social context is not required. It was, however, more effective than a 4 h presentation of synthesized, pseudorandom tones. When birds entrained to the period of the zeitgebers, their expressed period closely matched 24 h with phases closely matched to the onset of the zeitgeber. Masking was not evident in contrast to masking observed following transfer from constant dim light to LD and vice versa.This series of experiments could prove a means of quantifying the capacity for reciprocal social interaction, a state which can be dynamic in songbirds, as well as the integration between sociality and the circadian clock.

Physical activity reduces prostate carcinogenesis in a transgenic model.

BACKGROUND: Several epidemiological studies have reported an inverse association between physical activity and the risk of prostate cancer. To date, there are few animal studies looking at physical activity and cancer incidence, although the results are consistent with the epidemiological evidence. In general, as exercise intensity increased in the rats/mice, the likelihood that physical activity inhibited carcinogenesis increased. METHODS: The present study used voluntary wheel running with C3(1)Tag mice that are predisposed to prostate cancer due to the directed expression of SV40 oncogenes. After 10 weeks, the prostates were collected from run and non-run mice and histopathology performed for the presence or absence of low grade or high grade PINS. RESULTS: We found that for those mice that ran >5Km/group, 83% of the dorsolateral prostates were classified as within normal levels vs. 43% for the <5 km/day group (p = 0.16). In addition, there was a relationship between average distance run and pathologic progression to high-grade PIN and local invasion, considered to be an early event in the formation of prostate adenocarcinoma. Forty three percent of dorsalateral prostates from mice that ran less than 5 km/day were classified with advanced pathology as compared to none (0) in mice that ran more than 5.0 km/day (p = 0.05). CONCLUSIONS: The results presented herein indicate that prostatic cancer progression is likely delayed or diminished by wheel running activity in a dose dependent manner in transgenic mice. These findings provide further evidence that exercise acts to decrease the progression of prostate cancer and they establish a transgenic animal model for future studies to define mechanism(s). Prostate 69: 1372-1377, 2009. (c) 2009 Wiley-Liss, Inc.

Circadian regulation of bird song, call, and locomotor behavior by pineal melatonin in the zebra finch.

As both a photoreceptor and pacemaker in the avian circadian clock system, the pineal gland is crucial for maintaining and synchronizing overt circadian rhythms in processes such as locomotor activity and body temperature through its circadian secretion of the pineal hormone melatonin. In addition to receptor presence in circadian and visual system structures, high-affinity melatonin binding and receptor mRNA are present in the song control system of male oscine passeriform birds. The present study explores the role of pineal melatonin in circadian organization of singing and calling behavior in comparison to locomotor activity under different lighting conditions. Similar to locomotor activity, both singing and calling behavior were regulated on a circadian basis by the central clock system through pineal melatonin, since these behaviors free-ran with a circadian period and since pinealectomy abolished them in constant environmental conditions. Further, rhythmic melatonin administration restored their rhythmicity. However, the rates by which these behaviors became arrhythmic and the rates of their entrainment to rhythmic melatonin administration differed among locomotor activity, singing and calling under constant dim light and constant bright light. Overall, the study demonstrates a role for pineal melatonin in regulating circadian oscillations of avian vocalizations in addition to locomotor activity. It is suggested that these behaviors might be controlled by separable circadian clockworks and that pineal melatonin entrains them all through a circadian clock.