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INTRODUCTION: Optimal exploitation of the huge amounts of agro-industrial residuals that are produced annually, which endangers the ecosystem and ultimately contributes to climate change, is one of the solutions available to produce value-added compounds. AIM AND OBJECTIVES: This study aimed at the economic production and optimization of surfactin. Therefore, the production was carried out by the microbial conversion of Potato Peel Waste (PPW) and Frying Oil Waste (FOW) utilizing locally isolated Bacillus halotolerans. Also, investigating its potential application as an antimicrobial agent towards some pathogenic strains. RESULTS: Screening the bacterial isolates for surfactin production revealed that the strain with the highest yield (49 g/100 g substrate) and efficient oil displacement activity was genetically identified as B. halotolerans. The production process was then optimized utilizing Central Composite Design (CCD) resulting in the amelioration of yield by 11.4% (from 49 to 55.3 g/100 g substrate) and surface tension (ST) by 8.3% (from 36 to 33 mN/m) with a constant level of the critical micelle concentration (CMC) at 125 mg/L. Moreover, the physiochemical characterization studies of the produced surfactin by FTIR, 1H NMR, and LC-MS/MS proved the existence of a cyclic lipopeptide (surfactin). The investigations further showed a strong emulsification affinity for soybean and motor oil (E24 = 50%), as well as the ability to maintain the emulsion stable over a wide pH (4-10) and temperature (10-100 °C) range. Interestingly, surfactin had a broad-spectrum range of inhibition activity against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, klebsiella pneumonia, and Candida albicans. CONCLUSION: Subsequently, the screening of the isolates and the utilized food-processing wastes along with the extraction technique resulted in a high yield of surfactin characterized by acceptable ST and CMC levels. However, optimization of the cultural conditions to improve the activity and productivity was achieved using Factor-At-A-Time (OFAT) and Central Composite Design (CCD). In contrast, surface activity recorded a maximum level of (33 mN/n) and productivity of 55.3 g/100 g substrate. The optimized surfactin had also the ability to maintain the stability of emulsions over a wide range of pH and temperature. Otherwise, the obtained results proved the promising efficiency of the surfactin against bacterial and fungal pathogens.
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Bacillus , Resíduos Industriais , Lipopeptídeos , Solanum tuberosum , Bacillus/metabolismo , Bacillus/genética , Bacillus/isolamento & purificação , Lipopeptídeos/farmacologia , Lipopeptídeos/metabolismo , Lipopeptídeos/biossíntese , Lipopeptídeos/química , Lipopeptídeos/isolamento & purificação , Solanum tuberosum/microbiologia , Peptídeos Cíclicos/farmacologia , Peptídeos Cíclicos/química , Peptídeos Cíclicos/isolamento & purificação , Peptídeos Cíclicos/biossíntese , Testes de Sensibilidade Microbiana , Anti-Infecciosos/farmacologia , Anti-Infecciosos/metabolismo , Anti-Infecciosos/química , Anti-Infecciosos/isolamento & purificação , Agricultura/métodosRESUMO
BACKGROUND AND OBJECTIVES: Microbial cells capability to tolerate the effect of various antimicrobial classes represent a major worldwide health concern. The flexible and multi-components nanocomposites have enhanced physicochemical characters with several improved properties. Thus, different biological activities of biosynthesized starch/silver-selenium nanocomposite (St/Ag-Se NC) were assessed. METHODOLOGY: The St/Ag-Se NC was biosynthesized using Cladosporium cladosporioides CBS 174.62 (C. cladosporioides) strain. The shape and average particle size were investigated using scanning electron microscope (SEM) and high-resolution transmission electron microscope (HR-TEM), respectively. On the other hand, the St/Ag-Se NC effect on two cancer cell lines and red blood cells (RBCs) was evaluated and its hydrogen peroxide (H2O2) scavenging effect was assessed. Moreover, its effects on various microbial species in both planktonic and biofilm growth forms were examined. RESULTS: The St/Ag-Se NC was successfully biosynthesized with oval and spherical shape and a mean particle diameter of 67.87 nm as confirmed by the HR-TEM analysis. St/Ag-Se NC showed promising anticancer activity toward human colorectal carcinoma (HCT-116) and human breast cancer (MCF-7) cell lines where IC50 were 21.37 and 19.98 µg/ml, respectively. Similarly, little effect on RBCs was observed with low nanocomposite concentration. As well, the highest nanocomposite H2O2 scavenging activity (42.84%) was recorded at a concentration of 2 mg/ml. Additionally, Staphylococcus epidermidis (S. epidermidis) ATCC 12,228 and Candida albicans (C. albicans) ATCC 10,231 were the highly affected bacterial and fungal strains with minimum inhibitory concentrations (MICs) of 18.75 and 50 µg/ml, respectively. Moreover, the noticeable effect of St/Ag-Se NC on microbial biofilm was concentration dependent. A high biofilm suppression percentage, 87.5% and 68.05%, were recorded with S. epidermidis and Staphylococcus aureus (S. aureus) when exposed to 1 mg/ml and 0.5 mg/ml, respectively. CONCLUSION: The biosynthesized St/Ag-Se NC showed excellent antioxidant activity, haemocompatibility, and anti-proliferative effect at low concentrations. Also, it exhibited promising antimicrobial and antibiofilm activities.
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Anti-Infecciosos , Cladosporium , Nanopartículas Metálicas , Nanocompostos , Selênio , Humanos , Prata/farmacologia , Prata/química , Selênio/farmacologia , Amido/química , Peróxido de Hidrogênio/farmacologia , Staphylococcus aureus , Anti-Infecciosos/farmacologia , Nanopartículas Metálicas/química , Testes de Sensibilidade Microbiana , Antibacterianos/farmacologia , Antibacterianos/químicaRESUMO
Heavy metals (HMs) like Zn, Cu, Pb, Ni, Cd, and Hg, among others, play a role in several environmental problems. The marine environment is polluted by several contaminants, such as HMs. A variety of physico-chemical methods usually available for sanitation HMs remediation suffer from either limitation. Bioremediation is a promising way of dealing with HMs pollution. Microbes have the ability with various potencies to resist HMs tension. The current review discusses the main sources and influences of HMs, the role of marine microorganisms in HMs bioremediation, as well as the microbial mechanisms for HMs detoxification and transformation. This review paper aims to provide an overview of the bioremediation technologies that are currently available for the removal of HMs ions from industrial and urban effluent by aquatic organisms such as bacteria, fungi, and microalgae, particularly those that are isolated from marine areas. The primary goals are to outline various studies and offer helpful information about the most important aspects of the bioelimination techniques. The biotreatment practices have been primarily divided into three techniques based on this topic. They are biosorption, bioaccumulation, bioleaching, and biotransformation. This article gives the brief view on the research studies about bioremediation of HMs using marine microorganisms. The current review also deals with the critical issues and recent studies based on the HMs biodetoxification using aquatic microorganisms.
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Metais Pesados , Microalgas , Biodegradação Ambiental , IndústriasRESUMO
Bacterial Cellulose (BC) is still the most renewable available biopolymer produced in fine nature from alternative microbial sources as bacteria. In the present study, newly BC producing bacteria were successfully isolated from acidic fruits. The most potent producer was isolated from strawberry and identified genetically using 16 s rRNA technique as Achromobacter S3. Different fruit peels were screened to produce BC using the cheapest culture medium. Among them, Mango peel waste (MPW) hydrolysate proved to be the significant inducible alternative medium without any extra nutrients for the maximum productivity. Improvement of the BC yield was successfully achieved via statistical optimization of the MPW culture medium, from 0.52 g/L to 1.22 g/L with 2.5-fold increased about the standard HS culture medium. Additionally, the physicochemical analysis affirmed the cellulose molecular structure as well as observed the crystallinity of nanofiber as 72 and 79% for BC produced by Achromobacter S33 on HS and MPW media, respectively. Moreover, the topographical study illustrated that the BC nanofibers had close characteristics upon fiber dimeter and length as about 10 and 200 nm, respectively.
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Celulose , Mangifera , Biopolímeros , Meios de Cultura/químicaRESUMO
Purification of L-methionine γ-lyase (MGL) from A. fumigatus was sequentially conducted using heat treatment and gel filtration, resulting in 3.04 of purification fold and 73.9% of enzymatic recovery. The molecular mass of the purified MGL was approximately apparent at 46 KDa based on SDS-PAGE analysis. The enzymatic biochemical properties showed a maximum activity at pH 7 and exhibited plausible stability within pH range 5.0-7.5; meanwhile the highest catalytic activity of MGL was observed at 30-40 °C and the enzymatic stability was noted up to 40 °C. The enzyme molecule was significantly inhibited in the presence of Cu2+, Cd2+, Li2+, Mn2+, Hg2+, sodium azide, iodoacetate, and mercaptoethanol. Moreover, MGL displayed a maximum activity toward the following substrates, L-methionine < DL-methionine < Ethionine < Cysteine. Kinetic studies of MGL for L-methioninase showed catalytic activity at 20.608 mM and 12.34568 µM.min-1. Furthermore, MGL exhibited anticancer activity against cancerous cell lines, where IC50 were 243 ± 4.87 µg/ml (0.486 U/ml), and 726 ± 29.31 µg/ml (1.452 U/ml) against Hep-G2, and HCT116 respectively. In conclusion, A. fumigatus MGL had good catalytic properties along with significantly anticancer activity at low concentration which makes it a probably candidate to apply in the enzymotherapy field.
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Aspergillus fumigatus , Liases de Carbono-Enxofre , Aspergillus fumigatus/metabolismo , Cinética , Liases de Carbono-Enxofre/química , Liases de Carbono-Enxofre/metabolismo , MetioninaRESUMO
BRIEF INTRODUCTION: Mucormycosis disease, which has recently expanded with the Covid 19 pandemic in many countries, endangers patients' lives, and treatment with common drugs is fraught with unfavorable side effects. AIM AND OBJECTIVES: This study deals with the economic production of sophorolipids (SLs) from different eight fungal isolates strains utilizing potato peels waste (PPW) and frying oil waste (FOW). Then investigate their effect against mucormycetes fungi. RESULTS: The screening of the isolates for SLs production revealed the highest yield (39 g/100 g substrate) with most efficiency was related to a yeast that have been identified genetically as Candida parapsilosis. Moreover, the characterizations studies of the produced SLs by FTIR, 1H NMR and LC-MS/MS proved the existence of both acidic and lactonic forms, while their surface activity was confirmed by the surface tension (ST) assessment. The SLs production was optimized utilizing Box-Behnken design resulting in the amelioration of yield by 30% (55.3 g/100 g substrate) and ST by 20.8% (38mN/m) with constant level of the critical micelle concentration (CMC) at 125 mg/L. The studies also revealed the high affinity toward soybean oil (E24 = 50%), in addition to maintaining the emulsions stability against broad range of pH (4-10) and temperature (10-100â). Furthermore, the antifungal activity against Mucor racemosus, Rhizopus microsporus, and Syncephalastrum racemosum proved a high inhibition efficiency of the produced SLs. CONCLUSION: The findings demonstrated the potential application of the SLs produced economically from agricultural waste as an effective and safer alternative for the treatment of infection caused by black fungus.
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COVID-19 , Mucorales , Solanum tuberosum , Humanos , Candida parapsilosis , Cromatografia Líquida , Espectrometria de Massas em TandemRESUMO
Human life has been significantly impacted by the creation and spread of novel species of antibiotic-resistant bacteria and virus strains that are difficult to manage. Scientists and researchers have recently been motivated to seek out alternatives and other sources of safe and ecologically friendly active chemicals that have a powerful and effective effect against a wide variety of pathogenic bacteria as a result of all these hazards and problems. In this review, endophytic fungi and their bioactive compounds and biomedical applications were discussed. Endophytes, a new category of microbial source that can produce a variety of biological components, have major values for study and broad prospects for development. Recently, endophytic fungi have received much attention as a source for new bioactive compounds. In addition, the variety of natural active compounds generated by endophytes is due to the close biological relationship between endophytes and their host plants. The bioactive compounds separated from endophytes are usually classified as steroids, xanthones, terpenoids, isocoumarins, phenols, tetralones, benzopyranones and enniatines. Moreover, this review discusses enhancement methods of secondary metabolites production by fungal endophytes which include optimization methods, co-culture method, chemical epigenetic modification and molecular-based approaches. Furthermore, this review deals with different medical applications of bioactive compounds such as antimicrobial, antiviral, antioxidant and anticancer activities in the last 3 years.
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Anti-Infecciosos , Fungos , Humanos , Fungos/metabolismo , Plantas/microbiologia , Anti-Infecciosos/metabolismo , Endófitos/metabolismo , Antibacterianos/metabolismoRESUMO
Nanotechnology is playing a critical role in several essential technologies with nanoscale structures (nanoparticles) in areas of the environment and biomedicine. In this work, the leaf extract of Pluchea indica was utilized to biosynthesize zinc oxide nanoparticles (ZnONPs) for the first time and evaluated for antimicrobial and photocatalytic activities. Different experimental methods were used to characterize the biosynthesized ZnONPs. The biosynthesized ZnONPs showed maximum Ultraviolet-visible spectroscopy (UV-vis) absorbance at a wavelength of 360 nm. The X-Ray diffraction (XRD) pattern of the ZnONPs exhibits seven strong reflection peaks, and the average particle size was 21.9 nm. Fourier-transform infrared spectroscopy (FT-IR) spectrum analysis reveals the presence of functional groups that help in biofabrication. The existence of Zn and O was confirmed by the Energy-dispersive X-ray (EDX) spectrum and the morphology by SEM images. Antimicrobial studies showed that the biosynthesized ZnONPs have antimicrobial efficacy against Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, Bacillus subtilis, Staphylococcus aureus, Candida albicans and Cryptococcus neoformans where inhibition zones at concentration 1000 µg/mL were 21.83 ± 0.76, 13.0 ± 1.1, 14.9 ± 0.85, 24.26 ± 1.1, 17.0 ± 1.0, 20.67 ± 0.57 and 19.0 ± 1.0 mm respectively. Under both dark and sunlight irradiation, the photocatalytic activity of ZnONPs was evaluated towards the degradation of the thiazine dye (methylene blue-MB). Approximately 95% of the MB dye was broken down at pH 8 after 150 min of sunlight exposure. The aforementioned results, therefore, suggest that ZnONPs synthesized by implementing environmentally friendly techniques can be employed for a variety of environmental and biomedical applications.
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Anti-Infecciosos , Nanopartículas Metálicas , Óxido de Zinco , Antibacterianos/farmacologia , Antibacterianos/química , Óxido de Zinco/farmacologia , Óxido de Zinco/química , Espectroscopia de Infravermelho com Transformada de Fourier , Nanopartículas Metálicas/química , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Anti-Infecciosos/farmacologia , Escherichia coli , Testes de Sensibilidade Microbiana , Difração de Raios XRESUMO
In the current study, clove oil nanoemulsion (CL-nanoemulsion) and emulsion (CL-emulsion) were prepared through an ecofriendly method. The prepared CL-nanoemulsion and CL-emulsion were characterized using dynamic light scattering (DLS) and a transmission electron microscope (TEM), where results illustrated that CL-nanoemulsion droplets were approximately 32.67 nm in size and spherical in shape, while CL-nanoemulsion droplets were approximately 225.8 nm with a spherical shape. The antibacterial activity of CL-nanoemulsion and CL-emulsion was carried out using a microbroth dilution method. Results revealed that the preferred CL-nanoemulsion had minimal MIC values between 0.31 and 5 mg/mL. The antibiofilm efficacy of CL-nanoemulsion against S. aureus significantly decreased the development of biofilm compared with CL-emulsion. Furthermore, results illustrated that CL-nanoemulsion showed antifungal activity significantly higher than CL-emulsion. Moreover, the prepared CL-nanoemulsion exhibited outstanding antifungal efficiency toward Candida albicans, Cryptococcus neoformans, Aspergillus brasiliensis, A. flavus, and A. fumigatus where MICs were 12.5, 3.12, 0.78, 1.56, and 1.56 mg/mL, respectively. Additionally, the prepared CL-nanoemulsion was analyzed for its antineoplastic effects through a modified MTT assay for evaluating apoptotic and cytotoxic effects using HepG2 and MCF-7 cell lines. MCF-7 breast cancer cells showed the lowest IC50 values (3.4-fold) in CL-nanoemulsion relative to that of CL-emulsion. Thus, CL-nanoemulsion induces apoptosis in breast cancer cells by inducing caspase-8 and -9 activity and suppressing VEGFR-2. In conclusion, the prepared CL-nanoemulsion had antibacterial, antifungal, and antibiofilm as well as anticancer properties, which can be used in different biomedical applications after extensive studies in vivo.
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Antibacterianos , Antifúngicos , Antineoplásicos , Biofilmes , Óleos Voláteis , Syzygium , Biofilmes/efeitos dos fármacos , Antineoplásicos/química , Antineoplásicos/farmacologia , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Emulsões , Syzygium/química , Difusão Dinâmica da Luz , Microscopia Eletrônica de Transmissão , Células Hep G2 , Células MCF-7 , Humanos , Apoptose , Antibacterianos/química , Antibacterianos/farmacologia , Antifúngicos/química , Antifúngicos/farmacologia , Sistemas de Liberação de Fármacos por Nanopartículas , Nanoestruturas/química , Staphylococcus aureus/efeitos dos fármacos , Fungos/efeitos dos fármacosRESUMO
Essential oil nanoemulsions have received much attention due to their biological activities. Thus, a thyme essential oil nanoemulsion (Th-nanoemulsion) was prepared using a safe and eco-friendly method. DLS and TEM were used to characterize the prepared Th-nanoemulsion. Our findings showed that the nanoemulsion was spherical and ranged in size from 20 to 55.2 nm. The micro-broth dilution experiment was used to evaluate the in vitro antibacterial activity of a Th-emulsion and the Th-nanoemulsion. The MIC50 values of the thymol nanoemulsion were 62.5 mg/mL against Escherichia coli and Klebsiella oxytoca, 250 mg/mL against Bacillus cereus, and 125 mg/mL against Staphylococcus aureus. Meanwhile, it emerged that the MIC50 values of thymol against four strains were not detected. Moreover, the Th-nanoemulsion exhibited promising antifungal activity toward A. brasiliensis and A. fumigatus, where inhibition zones and MIC50 were 20.5 ± 1.32 and 26.4 ± 1.34 mm, and 12.5 and 6.25 mg/mL, respectively. On the other hand, the Th-nanoemulsion displayed weak antifungal activity toward C. albicans where the inhibition zone was 12.0 ± 0.90 and MIC was 50 mg/mL. Also, the Th-emulsion exhibited antifungal activity, but lower than that of the Th-nanoemulsion, toward all the tested fungal strains, where MIC was in the range of 12.5-50 mg/mL. The in vitro anticancer effects of Taxol, Th-emulsion, and Th-nanoemulsion were evaluated using the standard MTT method against breast cancer (MCF-7) and hepatocellular carcinoma (HepG2). Additionally, the concentration of VEGFR-2 was measured, and the activities of caspase-8 (casp-8) and caspase-9 (casp-9) were evaluated. The cytotoxic effect was the most potent against the MCF-7 breast cancer cell line after the Th-nanoemulsion treatment (20.1 ± 0.85 µg/mL), and was 125.1 ± 5.29 µg/mL after the Th-emulsion treatment. The lowest half-maximal inhibitory concentration (IC50) value, 20.1 ± 0.85 µg/mL, was achieved when the MCF-7 cell line was treated with the Th-nanoemulsion. In addition, Th-nanoemulsion treatments on MCF-7 cells led to the highest elevations in casp-8 and casp-9 activities (0.66 ± 0.042 ng/mL and 17.8 ± 0.39 pg/mL, respectively) compared to those with Th-emulsion treatments. In comparison to that with the Th-emulsion (0.982 0.017 ng/mL), the VEGFR-2 concentration was lower with the Th-nanoemulsion treatment (0.672 ± 0.019ng/mL). In conclusion, the Th-nanoemulsion was successfully prepared and appeared in nanoform with a spherical shape according to DLS and TEM, and also exhibited antibacterial, antifungal, as well as anticancer activities.
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Anti-Infecciosos , Neoplasias da Mama , Óleos Voláteis , Humanos , Feminino , Timol/farmacologia , Antifúngicos/farmacologia , Receptor 2 de Fatores de Crescimento do Endotélio Vascular , Emulsões/farmacologia , Anti-Infecciosos/farmacologia , Óleos Voláteis/farmacologia , Antibacterianos/farmacologia , Candida albicansRESUMO
Microalgae have garnered scientific interest for their potential to produce bioactive compounds. However, the large-scale industrial utilization of microalgae faces challenges related to production costs and achieving optimal growth conditions. Thus, this study aimed to investigate the potential role of exogenous indole-3-acetic acid (IAA) application in improving the growth and production of bioactive metabolites in microalgae. To this end, the study employed different concentrations of exogenously administered IAA ranging from 0.36 µM to 5.69 µM to assess its influence on the growth and biochemical composition of Synechocystis and Chlorella. IAA exposure significantly increased IAA levels in both strains. Consequentially, improved biomass accumulation in parallel with increased total pigment content by approximately eleven-fold in both strains was observed. Furthermore, the application of IAA stimulated the accumulation of primary metabolites. Sugar levels were augmented, providing a carbon source that facilitated amino acid and fatty acid biosynthesis. As a result, amino acid levels were enhanced as well, leading to a 1.55-fold increase in total amino acid content in Synechocystis and a 1.42-fold increase in Chlorella. Total fatty acids content increased by 1.92-fold in Synechocystis and by 2.16-fold in Chlorella. Overall, the study demonstrated the effectiveness of exogenously adding IAA as a strategy for enhancing the accumulation of microalgae biomass and biomolecules. These findings contribute to the advancement of microalgae-based technologies, opening new avenues to produce economically important compounds derived from microalgae.
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Chlorella , Microalgas , Synechocystis , Synechocystis/metabolismo , Ácidos Graxos/metabolismo , Ácidos Indolacéticos/farmacologia , Ácidos Indolacéticos/metabolismo , Aminoácidos/metabolismo , Microalgas/metabolismo , Biomassa , BiocombustíveisRESUMO
In this study, a novel, non-toxic, eco-friendly zinc oxide nanoparticles (ZnO-NPs) was used instead of the synthetic fungicides widely used to control the destructive phytopathogenic fungus Fusarium oxysporum, the causative agent of wilt disease in Solanum melongena L. Herein, the biosynthesized ZnO-NPs was carried out by Penicillium expansum ATCC 7861. In vitro, mycosynthesized ZnO-NPs exhibited antifungal activity against Fusarium oxysporum. In vivo, ZnO-NPs suppressed Fusarium wilt disease in cultivated Solanum melongena L. by decreasing the disease severity with 75% of plant protection. Moreover, ZnO-NPs stimulated the recovery of eggplant as an indicated by improving of morphological and metabolic indicators including plant height(152.5%), root length(106.6%), plant fresh biomass (146%), chlorophyll a (102.8%), chlorophyll b (67.86%), total soluble carbohydrates (48.5%), total soluble protein (81.8%), phenol (10.5%), antioxidant activity and isozymes compared with infected control. Therefore, this study suggests using mycosynthesized ZnO-NPs as an alternative to synthetic fungicides not only to eradicate the Fusarium wilt disease in cultivated eggplant (Solanum melongena) but also to promote the growth parameters and metabolic aspects.
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Fungicidas Industriais , Fusarium , Nanopartículas , Solanum melongena , Óxido de Zinco , Clorofila A , Fungicidas Industriais/farmacologia , Solanum melongena/microbiologia , Óxido de Zinco/farmacologiaRESUMO
Background and Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) isolates are associated with various diseases ranged from mild superficial impairments to invasive infections. This study aimed to evaluate the ability of polymerase chain reaction (PCR) based methods namely, restriction fragment length polymorphism (RFLP) of the coa gene and random amplified polymorphic DNA (RAPD), to determine the genetic diversity of MRSA isolates. Materials and Methods: A total of 37 MRSA isolates were conventionally identified depending on their biochemical and microbiological culture characteristics. Genotypic confirmation was based on detection of the associated mecA gene. The genetic variation amongst MRSA isolates was evaluated following the coa gene-based RFLP and RAPD fingerprints. Results: Results illustrated that, the species specific coa gene was detected in all MRSA isolates. The irregular bands intensity, number, and molecular sizes of the PCR amplicons demonstrated the coa gene polymorphism. The incompatible AluI digestion patterns of these amplicons classified the tested MRSA isolates into 20 RFLP patterns which confirm the coa gene polymorphism. Additionally, the PCR-based RAPD analysis showed variable bands number with size range of approximately 130 bp to 4 kbp, which indicated the genetic variation of the tested MRSA isolates as it created 36 variable RAPD banding profiles. Conclusions: coa gene AluI enzymatic restriction sites, amongst the tested MRSA isolates, certify their genetic variation on the basis of the accurate but complicated and relatively expensive coa gene-based RFLP. Conversely, the results verified the excellent ability of the simple and cost-effective PCR-based RAPD analysis to discriminate between MRSA isolates without any preface data about the genome.
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Staphylococcus aureus Resistente à Meticilina , Infecções Estafilocócicas , Coagulase/genética , Coagulase/farmacologia , Coenzima A/genética , Coenzima A/farmacologia , DNA/farmacologia , Humanos , Staphylococcus aureus Resistente à Meticilina/genética , Polimorfismo de Fragmento de Restrição , Técnica de Amplificação ao Acaso de DNA Polimórfico , Infecções Estafilocócicas/diagnóstico , Staphylococcus aureus/genéticaRESUMO
In the present work, sustainable and green method was used to prepare silver nanoparticles (Ag-NPs), followed with incorporation into tertiary nanocomposite consisted of starch, oxidized cellulose and ethyl cellulose. The prepared tertiary silver-nanocomposite (Ag-NC) was fully characterized via instrumental analysis (UV-vis, FT-IR, XRD, SEM, EDX and TEM) and evaluated for antibacterial, antifungal, and antiviral activities. Ag-NC significantly suppressed growth of tested bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis) as compared with controls. Antifungal activity revealed that the prepared tertiary Ag-NC has a promising antifungal activity towards unicellular (Candida albicans) and multicellular fungi ( Aspergillus niger, A. terreus, A. flavus and A. fumigatus). In same line, both Ag-NC and free Ag-NPs have shown a dose-dependent reduction in Vero cell line with maximum non-toxic dose at 6.25 and 12.5 µg/mL, respectively. Both Ag-NPs and Ag-NC exhibited antiviral effects against Herpes simplex virus, Adenovirus and Coxsackie B virus in a dose-dependent manner. Combined treatment of Ag-NPs incorporated into tertiary nanocomposite based on starch, oxidized cellulose and ethyl cellulose opens new possibilities to be more efficient nanomaterials for preventing microbial growth. In conclusion, the prepared tertiary Ag-NC has a promising antibacterial, antifungal as well as antiviral activities.
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Nanopartículas Metálicas , Nanocompostos , Antibacterianos/química , Antibacterianos/farmacologia , Antifúngicos/química , Antifúngicos/farmacologia , Antivirais/farmacologia , Bacillus subtilis/metabolismo , Nanopartículas Metálicas/química , Testes de Sensibilidade Microbiana , Nanocompostos/química , Prata/química , Prata/farmacologia , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
Expression of concern for 'Promising antimicrobial and antibiofilm activities of reduced graphene oxide-metal oxide (RGO-NiO, RGO-AgO, and RGO-ZnO) nanocomposites' by Sherif Elbasuney et al., RSC Adv., 2021, 11, 25961-25975, https://doi.org/10.1039/D1RA04542C.
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In this study, novel Cu-complexes of heterocyclic cellulose which were synthesized via the reaction of carboxymethyl cellulose (CMC) from bagasse pulp with NH2NH2 to give hydrazide cellulose which easily reacted with CS2 to form salt and then cyclized in the presence of HCl to afford cellulose oxadiazole, or with hydrazine hydrate to give cellulose triazole. Furthermore, the cellulose oxadiazole and triazole moieties acting as chelating agents with metal ion Cu (II), and all synthesized compounds were examined for their spectral analysis to show the adsorption of Cu (II) on the surface of cellulose through intramolecular hydrogen bonding. Results illustrated that cellulose oxadiazole and Cu- cellulose oxadiazole exhibited antimicrobial activities more than triazole and Cu- cellulose triazole. Furthermore, anticancer results showed that both cellulose oxadiazole and triazole exhibited activity higher than Cu-cellulose oxadiazole and Cu-cellulose triazole, where the cellulose triazole showed the highest activity (IC50â¯=â¯58.7⯵g/µL). Additionally, the docking simulation of the synthesized cellulose complexes with different proteins such as PDBID:3t88, PDBID:4ynt, PDBID:1tgh, PDBID:2wje, and PDBID:4hdq and shortage bond length to confirm the experimental results. Optimization of metal complexes utilized the DFT/B3LYP/LANL2DZ basis set to confirm the stability of these metals theoretically and their physical descriptors and FMO analysis.
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Anti-Infecciosos , Antineoplásicos , Celulose , Cobre , Simulação de Acoplamento Molecular , Celulose/química , Cobre/química , Antineoplásicos/química , Antineoplásicos/farmacologia , Anti-Infecciosos/farmacologia , Anti-Infecciosos/química , Humanos , Complexos de Coordenação/química , Complexos de Coordenação/farmacologia , Complexos de Coordenação/síntese química , Teoria da Densidade Funcional , Testes de Sensibilidade Microbiana , Compostos Heterocíclicos/química , Compostos Heterocíclicos/farmacologia , Linhagem Celular TumoralRESUMO
In the current study, endophytic Aspergillus hiratsukae was used for the biosynthesis of silver nanoparticles (Ag-NPs) for the first time. The characterizations were performed using X ray diffraction (XRD), Transmission electron microscopy (TEM), Scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX), Dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), and UV-Vis spectroscopy. The obtained results demonstrated the successful formation of crystalline, spherical Ag-NPs with particle diameters ranging from 16 to 31 nm. The FT-IR studied and displayed the various functional groups involved, which played a role in capping and reducing agents for Ag-NPs production. The SEM-EDX revealed that the main constituent of the AS-formed sample was primarily Ag, with a weight percentage of 64.2%. The mycosynthesized Ag-NPs were assessed for antimicrobial as well as photocatalytic activities. The antimicrobial results indicated that the synthesized Ag-NPs possess notable antibacterial efficacy against Staphylococcus aureus, Bacillus subtilis, and Escherichia coli, with minimum inhibitory concentrations (MICs) of Ag-NPs ranging from 62.5 to 250 µg/mL. Moreover, the biosynthesized Ag-NPs demonstrated weak antifungal activity against Aspergillus brasiliensis and Candida albicans, with MICs of 500 and 1,000 µg/mL, respectively. In addition, the mycosynthesized Ag-NPs exhibited photocatalytic activity toward acid black 2 (nigrosine) dye under both light and dark stimulation. Notably, After 300 min exposure to light, the nigrosine dye was degraded by 93%. In contrast, 51% degradation was observed after 300 min in darkness. In conclusion, Ag-NPs were successfully biosynthesized using endophytic A. hiratsukae and also exhibited antimicrobial and photocatalytic activities that can be used in environmental applications.
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Microbial resistance is the first morbidity and mortality cause for patients as usually a secondary infection. Additionally, the MOF is a promising material that shows a nice activity in this field. However, these materials need a good formulation to enhance biocompatibility and sustainability. Cellulose and its derivatives are well as filers for this gap. In this presented work, a novel green active system based on carboxymethyl cellulose and Ti-MOF (MIL-125-NH2@CMC) modified with thiophene (Thio@MIL-125-NH2@CMC) was prepared by a post-synthetic modification (PSM) route based. FTIR, SEM and PXRD were utilized to characterize nanocomposites. In addition, transmission electron microscopy (TEM) was used to corroborate the nanocomposites' particle size and diffraction pattern as well as the DLS affirmed the size as 50 and 35 nm for MIL-125-NH2@CMC and Thio@MIL-125-NH2@CMC, respectively. The formulation of the nanocomposites was validated by physicochemical characterization techniques, while morphological analysis confirmed the nanoform of the prepared composites. The antimicrobial, antiviral and antitumor properties of MIL-125-NH2@CMC and Thio@MIL-125-NH2@CMC were assessed. Antimicrobial testing revealed that Thio@MIL-125-NH2@CMC possesses greater antimicrobial activity than MIL-125-NH2@CMC. Additionally, Thio@MIL-125-NH2@CMC demonstrated promising antifungal activity against C. albicans and A. niger where MICs were 31.25 and 0.97 µg/mL, respectively. Also, Thio@MIL-125-NH2@CMC exhibited antibacterial activity against E. coli and S. aureus where MICs were 1000 and 250 µg/mL, respectively. In addition, the results demonstrated that Thio@MIL-125-NH2@CMC displayed promising antiviral activity against both HSV1 and COX B4, with antiviral activities of 68.89% and 39.60%, respectively. Furthermore, Thio@MIL-125-NH2@CMC exhibited potential anticancer activity against MCF7 and PC3 cancerous cell lines, where IC50 was 93.16 and 88.45%, respectively. In conclusion, carboxymethyl cellulose/sulfur-functionalized Ti-based MOF composite was successfully synthesized which had antimicrobial, antiviral and anticancer activities.