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1.
Trop Anim Health Prod ; 46(5): 739-45, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24633657

RESUMO

Caprine brucellosis is a bacterial zoonotic infection affecting goats especially in developing countries all over the world. In Malaysia, the risk factors associated with this infection in farms have not been studied. A case-control study was carried out in goat farms in four states of Malaysia to elucidate the risk factors associated with the infection on the farms using structured questionnaires and face-to-face interviews. Results indicate that the introduction of new animals (OR = 5.25; 90 % CI = 1.46, 18.88), younger age category of farms (OR = 5.53; 90 % CI = 1.09, 21.66), and farms with single breed of goats (OR = 8.50; 90 % CI = 1.27, 41.97) were significant risk factors for brucellosis. In order to control brucellosis or possibly eradicate it in goat farms, these factors need to be dealt with. Enforcing stringent importation protocols or complete ban of goat importation from brucellosis endemic countries will help reduce risk of introducing new infection into the country.


Assuntos
Brucella melitensis , Brucelose/veterinária , Doenças das Cabras/microbiologia , Aborto Animal/epidemiologia , Aborto Animal/microbiologia , Criação de Animais Domésticos , Animais , Estudos de Casos e Controles , Feminino , Doenças das Cabras/epidemiologia , Cabras , Malásia/epidemiologia , Fatores de Risco , Zoonoses
2.
Anal Sci ; 32(5): 543-7, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27169654

RESUMO

An on-cell aptamer sensor has the potential to reveal cell-cell communications by signalling molecules. We attempted to design new fluorescent aptamer sensors for the sensing of IFN-γ and adenine compounds on cells. BODIPY-labeled external quencher-free aptamer sensors have allowed a turn-on detection of the target molecule with improved off/on efficiency.


Assuntos
Adenina/análise , Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais/métodos , Compostos de Boro/química , Citometria de Fluxo/métodos , Corantes Fluorescentes/química , Interferon gama/análise , Adenina/química , Células Cultivadas , Humanos , Interferon gama/química , Estrutura Molecular
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