RESUMO
The Jacobin is a breed of fancy pigeon developed over many years of selective breeding that originated in Asia. In the present work, we report the complete mitochondrial genome sequence of Jacobin pigeon for the first time. The total length of the mitogenome was 17,245 bp with the base composition of 30.18% for A, 23.98% for T, 31.88% for C, and 13.96% for G and an A-T (54.17 %)-rich feature was detected. It harbored 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 1 non-coding control region. The arrangement of all genes was identical to the typical mitochondrial genomes of pigeon. The complete mitochondrial genome sequence of Jacobin pigeon would serve as an important data set of the germplasm resources for further study.
Assuntos
Columbidae/genética , Genoma Mitocondrial , Animais , Composição de Bases , Fases de Leitura Aberta/genética , RNA Ribossômico/genética , RNA de Transferência/genética , Análise de Sequência de DNARESUMO
The ice pigeon is a breed of fancy pigeon developed over many years of selective breeding. In the present work, we report the complete mitochondrial genome sequence of ice pigeon for the first time. The total length of the mitogenome was 17,236 bp with the base composition of 30.2% for A, 24.0% for T, 31.9% for C, and 13.9% for G and an A-T (54.2 %)-rich feature was detected. It harbored 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 1 non-coding control region (D-loop region). The arrangement of all genes was identical to the typical mitochondrial genomes of pigeon. The complete mitochondrial genome sequence of ice pigeon would serve as an important data set of the germplasm resources for further study.
Assuntos
Columbidae/genética , Genoma Mitocondrial , Mitocôndrias/genética , Animais , Composição de Bases , Cruzamento , Ordem dos Genes , RNA Ribossômico/genética , RNA de Transferência/genética , Análise de Sequência de DNARESUMO
The king pigeon is a breed of pigeon developed over many years of selective breeding primarily as a utility breed. In the present work, we report the complete mitochondrial genome sequence of king pigeon for the first time. The total length of the mitogenome was 17,221 bp with the base composition of 30.14% for A, 24.05% for T, 31.82% for C, and 13.99% for G and an A-T (54.22 %)-rich feature was detected. It harbored 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and one non-coding control region (D-loop region). The arrangement of all genes was identical to the typical mitochondrial genomes of pigeon. The complete mitochondrial genome sequence of king pigeon would serve as an important data set of the germplasm resources for further study.
Assuntos
Columbidae/genética , Genoma Mitocondrial , Animais , Composição de Bases , Fases de Leitura Aberta/genética , RNA Ribossômico/genética , RNA de Transferência/genética , Análise de Sequência de DNARESUMO
A sustainable approach that highly mimics bone-material deposition is reported to produce mechanically stable, degradable composites with nanostructures resembling that of natural bone. Molecular self-assembly combining intermolecular crosslinking leads to resilient matrices possessing long-range ordered aqueous domains, inside which moderately aligned poorly crystalline apatite is converted from the transient amorphous calcium phosphate phase.
Assuntos
Materiais Biomiméticos/química , Osso e Ossos/química , Nanocompostos/química , Apatitas/química , Modelos Moleculares , Conformação Molecular , Nanopartículas/químicaRESUMO
As pigs are susceptible to infection with both avian and human influenza A viruses, they have been proposed to be an intermediate host for the generation of pandemic virus through reassortment. The broad susceptibility of pigs to influenza viruses emphasizes the importance of surveillance of swine influenza virus. Thus, A latex agglutination test (LAT) was developed for rapid detection of antibodies to swine influenza virus. The nucleoprotein (NP) gene of the H9N2 swine influenza virus isolated from local farms was cloned, and expressed in Escherichia coli. Reactivity of the expressed protein was confirmed by Western blot. Subsequently, the NP gene was purified and used as the diagnostic antigen to develop a NP-based LAT for detecting antibodies to swine influenza virus. The LAT is shown to be specific for swine influenza virus and does not cross-react with swine sera that have antibodies to other swine viruses. The NP-LAT and HI test had a high agreement ratio in detecting 10 serum samples from naïve pigs, 28 serum samples from experimentally infected and vaccinated pigs. Compared with the hemagglutination inhibition (HI) test, the corresponding specificity, sensitivity, and correlation were 92.9%, 94.1%, and 94.1%, respectively, in detecting 321 serum samples from vaccinated pigs. The NP-LAT developed in our laboratory is a rapid and simple test suitable for field monitoring of antibodies to swine influenza virus. We conclude that it was specific and sensitive and it has great application potential in China's long-term prevention and control of swine influenza virus.