3D Dirac semimetal supported thermal tunable terahertz hybrid plasmonic waveguides.

By depositing the trapezoidal dielectric stripe on top of the 3D Dirac semimetal (DSM) hybrid plasmonic waveguide, the thermal tunable propagation properties have been systematically investigated in the terahertz regime, taking into account the influences of the structure of the dielectric stripe, temperature and frequency. The results manifest that as the upper side width of the trapezoidal stripe increases, the propagation length and figure of merit (FOM) both decrease. The propagation properties of hybrid modes are closely associated with temperature, in that when the temperature changes in the scope of 3-600 K, the modulation depth of propagation length is more than 96%. Additionally, at the balance point of plasmonic and dielectric modes, the propagation length and FOM manifest strong peaks and indicate an obvious blue shift with the increase of temperature. Furthermore, the propagation properties can be improved significantly with a Si-SiO2 hybrid dielectric stripe structure, e.g., on the condition that the Si layer width is 5 µm, the maximum value of the propagation length reaches more than 6.46 × 105 µm, which is tens of times larger than those pure SiO2 (4.67 × 104 µm) and Si (1.15 × 104 µm) stripe. The results are very helpful for the design of novel plasmonic devices, such as cutting-edge modulator, lasers and filters.

3D Dirac semimetals-dielectric elliptical fiber supported tunable terahertz hybrid waveguide.

Based on the proposed elliptical dielectric fiber-polyethylene gap-3D Dirac semimetal (DSM) hybrid plasmonic waveguide structure, the tunable propagation characteristics have been systematically investigated in the terahertz region, taking into account the influences of the structural parameters, the modified dielectric fiber, and the 3D DSM Fermi levels. The results show that as the ratio of the elliptical semi-axis along the y-direction ay and the x-direction ax (ay/ax) increases, the hybrid mode confinement increases. The real part of the effective mode index and propagation length increase with increasing the refractive index of the elliptical fiber. The propagation length and figure of merit of the hybrid modes reach 1.56×104µm and 300, respectively. In addition, by changing the Fermi level of the 3D DSM layer, the propagation properties of the hybrid modes can also be modulated in a wide range, e.g., the modulation depth of the propagation length reaches about 71.53% if the Fermi level varies in the range of 0.03-0.15 eV. The propagation properties of the hybrid modes are enhanced significantly by utilizing the modified three elliptical fiber structures, the real part of the effective mode index, and the propagation length of the modified structure are enhanced simultaneously. The results are very helpful for understanding the tunable mechanism of the 3D DSM devices and aids the design of novel plasmonic devices, e.g., lasers, modulators, and resonators.

Tunable 3D Dirac-semimetals supported mid-IR hybrid plasmonic waveguides.

Based on 3D Dirac-semimetal (DSM) modified hybrid waveguides, tunable propagation properties have been investigated, including the effects of Fermi levels, structural parameters, and operation frequency. The results show that if the operation frequency is smaller (larger) than the transition frequency (ℏω≈2|µc|), the proposed hybrid waveguides indicate strong (weak) confinement because the DSM layer manifests a high plasmonic (dielectric low) loss property. The dielectric fiber shape affects the propagation property obviously, as the elliptical parameter decreases, the confinement and figure of merit increase, and the loss reduces. With the increase in Fermi level, the propagation constant increases, and the frequency (amplitude) modulation depth is 32.31% (12.93%) if the Fermi level changes in the range of 0.01-0.15 eV. The results are very helpful in understanding the tunable mechanisms of hybrid waveguides and designing novel plasmonic devices in the future, e.g., modulators, filters, lasers, and resonators.

Quasi-bound states in the continuum in metal complementary periodic cross-shaped resonators at terahertz frequencies.

We numerically and experimentally demonstrate quasi-bound states in the continuum (BICs) in free-standing metal complementary periodic cross-shaped resonators (CPCRs) at terahertz frequencies. Such induced quasi-BICs arise from the broken symmetry in CPCRs. By slightly breaking mirror symmetry through reducing the length of one arm in CPCRs, the measured Q-factor of the quasi-BIC can reach 102, which is significantly high compared with that of conventional modes. Further study shows that the high Q-factor of the quasi-BIC is mainly determined not by the radiative loss but the material loss, as the asymmetry of the structures is unremarkable. The sharp quasi-BICs realized in the proposed structure may immediately boost the exploration of ultra-narrowband filters and ultra-sensitive sensors at terahertz frequencies.

Graphene-supported tunable bidirectional terahertz metamaterials absorbers.

Based on asymmetric graphene ellipses, the tunable propagation characteristics of metamaterial absorber (MMA) have been investigated in the THz region. Two distinct absorption peaks of 84% and 90% are observed at 1.06 THz and 1.67 THz, respectively. Besides a high Q factor exceeding 20, the Fano resonance can also be modulated in a wide range (e.g., the frequency modulation depth reaches more than 43.8% if the Fermi energy level changes in the range of 0.2-1.0 eV). Additionally, a bidirectional THz MMA is achieved by replacing the metal substrate with a uniform graphene layer. If the terahertz wave is incident in the forward direction, the proposed graphene double stripe microstructure shows a typical MMA with its absorption reaching 88%. On the other hand, if the terahertz wave is incident in the reverse direction, the graphene double stripe microstructure behaves as a reflective modulator, and its amplitude and frequency MD will reach 60% and 85%. These results contribute to the design of tunable THz devices, such as filters, absorbers, and modulators.

Identification of DNA hydroxymethylation associated genes in osteoarthritis by combined analysis of hydroxymethylation and gene expression.

BACKGROUND: Our study aimed to explore more mechanistic insights into the epigenetic regulation of osteoarthritis (OA). METHODS: The expression profiles (accession number: GSE64393 and GSE64394) were downloaded from the Gene Expression Omnibus database. The differentially hydroxymethylated regions (DhMRs) and differentially expressed genes (DEGs) between OA and control groups were identified. The distribution of DhMRs in the whole genome and the correlation between DhMRs and DEGs were analyzed. Functional module mining for the DEGs and DhMRs was conducted, followed by protein-protein interaction (PPI) analysis. The transcriptional factor (TF) was predicted. RESULTS: Total 52,282 DhMRs were obtained, among which 31,452 ones were annotated to 9726 genes. Additionally, 1806 DEGs were selected. Hydroxymethylation mainly occurred in gene body region. Correlation analysis revealed that more than 70% of DhMRs were uncorrelated with DEGs expression. Functional module mining for the DEGs and DhMRs identified 2 functional modules, which were involved in pathways of regulation of actin cytoskeleton, and TGF-ß signaling pathway. A PPI network was constructed, and ITGB3 had the highest degree. Furthermore, 7 TFs were predicted, which regulated 12 candidate genes, such as HES1-PTEN. CONCLUSIONS: The onset and progression of OA may be associated with the upregulated hydroxymethylation in gene body region of PTEN. HES1 may be important TF in the pathogenesis of OA. Additionally, pathways of regulation of actin cytoskeleton, and TGF-beta signaling pathway may also play important roles in OA progression.

Investigation of terahertz all-dielectric metamaterials.

The propagation properties of Si-based all-dielectric metamaterials (ADMs) structures were investigated systematically, taking into account the effects of structural parameters, operation frequencies, and graphene Fermi levels. The results manifested that ADMs indicated sharp resonant curves with large Q-factors of more than 60, and a figure of merit of approximately 20. Compared with that of thin metal metamaterial counterparts, the thickness of ADMs (in the range of tens of micrometers) required to excite obvious resonant curves was much larger. By introducing an asymmetrical structure, an obvious Fano-resonant peak was observed, which also became stronger with increasing asymmetrical degree. In addition, by unitizing a uniform graphene layer, the Fano-resonant curves can be flexibly modulated over a wide range, and the amplitude-modulation depth of the Fano peak was approximately 40% when the Fermi level varied in the range of 0.01-1.0 eV. These results are very useful for the design of high Q-factor dielectric devices in the future (e.g., biosensors, modulators, and filters).

Tunable MoS2 modified hybrid surface plasmon waveguides.

The tunable propagation properties of MoS2 supported hybrid surface plasmon waveguides based on dielectric fiber-gap-metal substrate structures have been investigated by using the finite element method, including the effects of structural parameters, the dielectric fiber shape and carrier concentration of the MoS2 layer. The results reveal that as the dielectric fiber radius increases, the confinement of the hybrid mode increases, and the losses show a peak. The shape of the dielectric fiber affects the propagation properties obviously, with an optimum structural parameter (a large value of the elliptical parameter) the confinement and figure of merits increase, and the dissipation decreases simultaneously. In addition, as the carrier concentration of the MoS2 layer increases, the modulation depth of absorption reaches more than 40%, and the propagation constants manifest obvious double peaks at wavelengths of 610 nm (2.03 eV) and 660 nm (1.88 eV), coming from the excitons' absorption of the MoS2 layer. The results are very useful in helping one to understand the tunable mechanisms of hybrid mode waveguide structures and for the design of novel surface plasmonic devices in the future, e.g. absorbers, modulators, lasers, and resonators.

Application of fiber optic high repetition rate laser-ablation spark-induced breakdown spectroscopy on the elemental analysis of aluminum alloys.

Fiber optic high repetition rate laser-ablation spark-induced breakdown spectroscopy was applied to realize elemental analysis of aluminum alloys. A compact fiber laser was used as source of laser-ablation and spark discharge was used to enhance the atomic emission of the laser-induced plasma. Plasma emission spectra were recorded with a compact fiber spectrometer in a nongated signal recording mode. Calibration curves of Cr, Cu, Mn, Mg, and Zn in aluminum alloys were built under appropriate experimental conditions and the detection limits of these elements were determined to be 4.4, 5.6, 4.9, 8.3, and 31.1 ppm, respectively. Compared to those obtained in fiber optic high repetition rate laser-induced breakdown spectroscopy using the same fiber laser, a 3-18 improvement factor in the detection limit has been demonstrated. This system is compact and cost effective, and the technique can be applied to rapid and convenient element analysis of different alloy samples.

Graphene patterns supported terahertz tunable plasmon induced transparency.

The tunable plasmonic induced transparency has been theoretically investigated based on graphene patterns/SiO2/Si/polymer multilayer structure in the terahertz regime, including the effects of graphene Fermi level, structural parameters and operation frequency. The results manifest that obvious Fano peak can be observed and efficiently modulated because of the strong coupling between incident light and graphene pattern structures. As Fermi level increases, the peak amplitude of Fano resonance increases, and the resonant peak position shifts to high frequency. The amplitude modulation depth of Fano curves is about 40% on condition that the Fermi level changes in the scope of 0.2-1.0 eV. With the distance between cut wire and double semi-circular patterns increases, the peak amplitude and figure of merit increases. The results are very helpful to develop novel graphene plasmonic devices (e.g. sensors, modulators, and antenna) and find potential applications in the fields of biomedical sensing and wireless communications.

Tunable high Q-factor terahertz complementary graphene metamaterial.

Based on the complementary graphene asymmetric double bars patterns, the tunable Fano resonances with large Q-factors have been investigated in the terahertz regime, including the effects of Fermi levels, structural parameters and operation frequency. The results reveal that compared with existed graphene tunable devices, the Fano resonant curve is very narrow and indicates a large Q-factor of about 60. The strong Fano resonant curves can be convenient tailored. As Fermi level increases, the amplitude of the Fano dip decreases, and the resonant peak position shifts to high frequency. The amplitude modulation depth (MD) of the Fano curve is more, about 90%, if the Fermi level changes in the scope of 0.2-1.0 eV. With the increase of the sample refractive index, the resonant Fano dip shifts low frequency, and the dip amplitude MD can reach more than 40%. The results are very helpful to understand the tunable mechanisms of graphene based Fano systems and to design high sensitivity functional devices, e.g. sensors, modulators, and antenna.

Switchable metamaterial for enhancing and localizing electromagnetic field at terahertz band.

In this article, a novel metamaterial is designed aimed at generating a single electromagnetic hot spot, in order to realize the localization of the incident electromagnetic field at terahertz band, and this kind of metastructure is an ideal candidate for many research fields such as spintronics, nonlinear magnetic response, near-field optics, and optical antenna, etc. The specially tailored metamaterial takes the shape of diabolo with a metal triangle pair connected by a cubic gallium arsenide (GaAs) gap. We demonstrated by simulation that both electric- and magnetic-field of incident THz pulse can be confined in the small GaAs gap when a synchronized femtosecond laser pulse is illuminated. The numerical simulation results show that 2 orders of magnitude of field enhancement can be obtained for a 1-by-1 µm GaAs gap, and the field enhancement factor can also be further improved by tailoring the GaAs gap down to nanometer scale.

Terahertz tunable graphene Fano resonance.

By depositing graphene circular double rings (DR) on a SiO2/Si/polymer substrate, the tunable Fano resonance has been theoretically investigated in the terahertz regime, including the effects of the graphene Fermi level, structural parameters and operation frequency. The results demonstrate that the obvious Fano peak can be efficiently modulated because of strong coupling between the incident waves and graphene ribbons. As the Fermi level increases, the peak amplitude of the Fano curve increases, and the resonant peak position shifts to a high frequency. The amplitude modulation depth of the Fano curves is about 30% if the Fermi level changes in the scope of 0.1-1.0 eV. The optimum gap distance between the DR is about 8-12 µm, where the value of the figure of merit shows a peak. The results are very helpful in order to develop novel graphene plasmonic devices, e.g. sensors and modulators.

Graphene-supported tunable near-IR metamaterials.

By integrating the metallic metamaterials (MMs) with a graphene layer, the resonant properties of an active tunable device based on the metal-SiO(2)-graphene (MSiO(2)G) structure have been theoretically investigated in the near-IR spectral region. The results manifest that the influences of the graphene layer on the propagation properties are significant. Owing to the tunability of the Fermi level of graphene, the resonance of transmitted or reflected curves can be tuned in a wide range (160-193 THz). To an original metal unit cell structure, an elevated Fermi level of graphene layer enhances the resonance dips and shifts it to the higher frequency. Compared with the original structure, the corresponding complementary MMs structure shows a much sharper spectral curve and can be used to fabricate a switcher or filters. The results are very helpful for designing graphene plasmonic devices.

Graphene-supported tunable extraordinary transmission.

By depositing a graphene layer on the metallic film with subwavelength hole arrays, the tunable extraordinary transmission property based on the metal-dielectrics-graphene (MDG) structure has been investigated in the terahertz (THz) and near-infrared (NIR) regimes. The influences of operation frequency, composed materials, and the Fermi level of the graphene layer have been taken into account. The results show that by varying the Fermi level of the graphene layer, the transmission of the MDG structure can be tuned in a wide range and the modulation depth of the peak value of the transmission can reach more than 50%. But the tunable mechanisms in the THz and NIR regimes are quite different. In the infrared (THz) regime, the graphene behaves like the dielectric (metallic) layer; its dielectric constant decreases (increases) with the increase of Fermi level, resulting in the transmission increasing (decreasing). Compared with the metallic structure, the transmission of the semiconductor structure can also be modulated by using the doping or varying temperature; its peak position can also be changed in a much broader range. The results are very useful to understand the mechanism of the graphene plasmonic devices and to design novel filters, switchers, modulators, and sensors.

Selaginellin derivatives from Selaginella tamariscina and evaluation for anti-breast cancer activity.

A phytochemical investigation of Selaginella tamariscina led to the isolation of 17 selaginellin derivatives. Their inhibitory activities against breast cancer cells were screened, and preliminary structure-activity relationships were also established. Among them, dimeric selaginellin 17 showed potential activity against MDA-MB-231 cells with an IC50 value of 3.2 ± 0.1 µM, corresponding to 4-fold higher potency than the reference compound 5-FU (IC50 14.8 ± 0.2 µM). Mechanistic studies indicated that 17 could cause G2/M phase arrest in MDA-MB-231 cells and induce apoptosis accompanied by increased ROS levels.

Application of quantitative analysis for aluminum alloy in femtosecond laser-ablation spark-induced breakdown spectroscopy using one-point and multi-line calibration.

To achieve the quantitative elemental analysis of aluminum alloy samples, the one-point and multi-line calibration (OP-MLC) method and femtosecond laser-ablation spark-induced breakdown spectroscopy (fs-LA-SIBS) were combined in this study. An ultrafast Ti: sapphire laser with high single-pulse energy and high repetition-rate operation was used as the laser source to ablate the sample at a 1k Hz pulse repetition rate and achieve re-excitation using spark discharge. Subsequently, an enhanced spectral peak intensity was achieved, which increased the sensitivity and analytical capabilities of laser-induced breakdown spectroscopy (LIBS) technique. Considering only one confirmed sample, the OP-MLC method was used to calculate the concentration ratios of trace elements Mg, Cr, Cu, Mn and Zn in four aluminum alloy samples to the calibration samples by the integral intensity ratio of the elemental characteristic lines between the two samples. The average relative errors with the identified concentrations were 3.99%, 15.13%, 8.76%, 9.30% and 9.64%, respectively. The results showed that the combination of OP-MLC method and fs-LA-SIBS has good accuracy in the quantitative analysis of elements, and has the advantages of simplicity, rapidity and high sensitivity.

The application of an intraoral banana peel suturing model in surgical training of dental students.

OBJECTIVES: This study investigated the application of an intraoral banana peel suturing model in helping students to acquire intraoral surgical techniques. METHODS: This is a self-control study conducted from January 2021 to March 2021. An intraoral banana peel suturing model was implemented to provide oral suture experience for undergraduates majoring in stomatology. The sutures students placed in the model were photographed and evaluated blindly by a professional team using an established scoring system. Training scores were recorded before (training 1) and after 2 months of training (training 2). Linear regression was used to examine factors related to the scores. Suturing training was conducted in the School and Hospital of Stomatology at Peking University. A total of eighty-two students in Peking University School and Hospital of Stomatology were in their fourth pre-clinical year and followed a workshop on surgical sutures according to the curriculum. All students who should take this course were included, and the response rate was 100%. RESULTS: The mean training 2 score (23.04 ± 3.83) was higher than the mean training 1 score (13.94 ± 3.15). The training 1 score was not significantly correlated with any of the students' general characteristics. The training 2 score was correlated with the training 1 score and the cumulative duration of practice outside of class. CONCLUSION: The intraoral banana peel suturing model can be used for suture training, and dental students' suture ability was improved after using the banana peel for suture practice.

Linkages between Plant Community Composition and Soil Microbial Diversity in Masson Pine Forests.

Plant species identity influences soil microbial communities directly by host specificity and root exudates, and indirectly by changing soil properties. As a native pioneer species common in early successional communities, Masson pine (Pinus massoniana) forests are widely distributed in subtropical China, and play a key role in improving ecosystem productivity. However, how pine forest composition, especially the dominance of plant functional groups, affects soil microbial diversity remains unclear. Here, we investigated linkages among woody plant composition, soil physicochemical properties, and microbial diversity in forests along a dominance gradient of Masson pine. Soil bacterial and fungal communities were mainly explained by woody plant community composition rather than by woody species alpha diversity, with the dominance of tree (without including shrub) species and ectomycorrhizal woody plant species accounting for more of the variation among microbial communities than pine dominance alone. Structural equation modeling revealed that bacterial diversity was associated with woody plant compositional variation via altered soil physicochemical properties, whereas fungal diversity was directly driven by woody plant composition. Bacterial functional groups involved in carbohydrate and amino acid metabolism were negatively correlated with the availability of soil nitrogen and phosphorus, whereas saprotrophic and pathogenic fungal groups showed negative correlations with the dominance of tree species. These findings indicate strong linkages between woody plant composition than soil microbial diversity; meanwhile, the high proportion of unexplained variability indicates great necessity of further definitive demonstration for better understanding of forest-microbe interactions and associated ecosystem processes.

Association of WHSC1/NSD2 and T-cell infiltration with prostate cancer metastasis and prognosis.

Progress in immunotherapy for prostate cancer (PCa) lags that for other cancers, mainly because of limited immune infiltration in PCa. This study aimed to assess the feasibility of NSD2 as an immunotherapeutic target in PCa. Immunohistochemistry was performed to evaluate the expression pattern of NSD2 in 34 cases of benign prostatic hyperplasia (BPH), 36 cases of prostatic intraepithelial neoplasia (PIN), and 57 cases of PCa, including 19 cases of metastatic castration-resistant prostatic cancer (mCRPC). Single-cell RNA sequencing and gene set enrichment analysis (GSEA) were used to correlate NSD2 with certain downstream pathways. Furthermore, the Immuno-Oncology-Biological-Research (IOBR) software package was used to analyze the potential roles of NSD2 in the tumor microenvironment. We found that the positive expression rate of NSD2 increased progressively in BPH, PIN and PCa. mCRPC had the highest staining intensity for NSD2. High NSD2 expression was positively correlated with the infiltration level of CD4+ tumor-infiltrating lymphocytes (TILs) and negatively correlated with that of CD8+ TILs. Importantly, a new immune classification based on NSD2 expression and CD4+ TILs and CD8+ TILs was successfully used to stratify PCa patients based on OS.PSA and CD4+ TILs are independent risk factors for PCa bone metastasis. This study demonstrates a novel role for NSD2 in defining immune infiltrate on in PCa and highlights the great potential for its application in immunotherapy response evaluation for prostate malignancies.