RESUMO
BACKGROUND: The Arabidopsis RUS (ROOT UV-B SENSITIVE) gene family contains six members, each of which encodes a protein containing a DUF647 (domain of unknown function 647) that is commonly found in eukaryotes. Previous studies have demonstrated that RUS1 and RUS2 play critical roles in early seedling development. All six RUS genes are expressed throughout the plant, but little is known about the functional roles of RUS3, RUS4, RUS5 and RUS6. RESULTS: We used a reverse-genetic approach to identify knockout mutants for RUS3, RUS4, RUS5 and RUS6. Each mutant was confirmed by direct DNA sequencing and genetic segregation analysis. No visible phenotypic differences were observed in rus3, rus4, or rus5 knockout mutants under standard growth conditions, but rus6 knockout mutants displayed a strong embryo-lethal phenotype. Two independent knockout lines for RUS6 were characterized. The rus6 mutations could only be maintained through a heterozygote, because rus6 homozygous mutants did not survive. Closer examinations of homozygous rus6 embryos from rus6/ + parent plants revealed that RUS6 is required for early embryo development. Loss of RUS6 resulted in embryo lethality, specifically at the mid-globular stage. The embryo-lethality phenotype was complemented by a RUS6::RUS6-GFP transgene, and GFP signal was detected throughout the embryo. Histological analyses with the ß-glucuronidase reporter gene driven by the RUS6 promoter showed tissue- and development-specific expression of RUS6, which was highest in floral tissues. CONCLUSION: Our data revealed that RUS6 is essential for early embryo development in Arabidopsis, and that the RUS gene family functions in multiple stages of plant development.
Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/genética , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Arabidopsis/embriologia , Proteínas de Arabidopsis/genética , Peptídeos e Proteínas de Sinalização Intracelular/genética , Mutação , Fenótipo , Regiões Promotoras Genéticas/genéticaRESUMO
Active-learning pedagogies have been repeatedly demonstrated to produce superior learning gains with large effect sizes compared with lecture-based pedagogies. Shifting large numbers of college science, technology, engineering, and mathematics (STEM) faculty to include any active learning in their teaching may retain and more effectively educate far more students than having a few faculty completely transform their teaching, but the extent to which STEM faculty are changing their teaching methods is unclear. Here, we describe the development and application of the machine-learning-derived algorithm Decibel Analysis for Research in Teaching (DART), which can analyze thousands of hours of STEM course audio recordings quickly, with minimal costs, and without need for human observers. DART analyzes the volume and variance of classroom recordings to predict the quantity of time spent on single voice (e.g., lecture), multiple voice (e.g., pair discussion), and no voice (e.g., clicker question thinking) activities. Applying DART to 1,486 recordings of class sessions from 67 courses, a total of 1,720 h of audio, revealed varied patterns of lecture (single voice) and nonlecture activity (multiple and no voice) use. We also found that there was significantly more use of multiple and no voice strategies in courses for STEM majors compared with courses for non-STEM majors, indicating that DART can be used to compare teaching strategies in different types of courses. Therefore, DART has the potential to systematically inventory the presence of active learning with â¼90% accuracy across thousands of courses in diverse settings with minimal effort.
Assuntos
Aprendizagem Baseada em Problemas/normas , Ciência/educação , Ensino/normas , Humanos , Som , Estudantes , Tecnologia , Universidades/normasRESUMO
All sun-exposed organisms are affected by UV-B [(UVB) 280-320 nm], an integral part of sunlight. UVB can cause stresses or act as a developmental signal depending on its fluence levels. In plants, the mechanism by which high-fluence-rate UVB causes damages and activates DNA-repair systems has been extensively studied. However, little is known about how nondamaging low-fluence-rate UVB is perceived to regulate plant morphogenesis and development. Here, we report the identification of an Arabidopsis mutant, root UVB sensitive 1 (rus1), whose primary root is hypersensitive to very low-fluence-rate (VLF) UVB. Under standard growth-chamber fluorescent white light, rus1 displays stunted root growth and fails to form postembryonic leaves. Experiments with different monochromatic light sources showed that rus1 phenotypes can be rescued if the seedlings are allowed to grow in light conditions with minimum UVB. We determined that roots, not other organs, perceive the UVB signal. Genetic and molecular analyses confirmed that the root light-sensitive phenotypes are independent of all other known plant photoreceptors. Three rus1 alleles have been identified and characterized. A map-based approach was used to identify the RUS1 locus. RUS1 encodes a protein that contains DUF647 (domain of unknown function 647), a domain highly conserved in eukaryotes. Our results demonstrate a root VLF UVB-sensing mechanism that is involved in Arabidopsis early seedling morphogenesis and development.
Assuntos
Arabidopsis/crescimento & desenvolvimento , Folhas de Planta/crescimento & desenvolvimento , Raízes de Plantas/crescimento & desenvolvimento , Plântula/crescimento & desenvolvimento , Raios Ultravioleta , Alelos , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Mutação , Folhas de Planta/genética , Raízes de Plantas/genética , Raízes de Plantas/metabolismo , Locos de Características Quantitativas/fisiologia , Plântula/genética , Plântula/metabolismoRESUMO
Gene fragment swapping and site-directed mutagenesis are commonly required in dissecting functions of gene domains. While there are many approaches for seamless fusion of different gene fragments, new methods are yet to be developed to offer higher efficiency, better simplicity, and more affordability. In this study, we showed that in most cases overlap-PCR was highly effective in creating site-directed mutagenesis, gene fragment deletion, and substitutions using RUS1 and RUS2 as example. While for cases where the overlap-PCR approach is not feasible due to complex secondary structure of gene fragments, a unique restriction site can be generated at the overlapped region of the primers through synonymous mutations. Then different gene fragments can be seamlessly fused through traditional restriction digestion and subsequent ligation. In conclusion, while the classical overlap-PCR is not feasible, the modified overlap-PCR approaches can provide effective and alternative ways to seamlessly fuse different gene fragments.
Assuntos
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Fusão Gênica Artificial/métodos , Substituição de Aminoácidos , Arabidopsis/química , Proteínas de Arabidopsis/química , Enzimas de Restrição do DNA/metabolismo , DNA de Plantas/química , Mutagênese Sítio-Dirigida , Conformação de Ácido Nucleico , Reação em Cadeia da Polimerase/métodos , Deleção de SequênciaRESUMO
Over the past few years, three photorespiratory bypasses have been introduced into plants, two of which led to observable increases in photosynthesis and biomass yield. However, most of the experiments were carried out using Arabidopsis under controlled environmental conditions, and the increases were only observed under low-light and short-day conditions. In this study, we designed a new photorespiratory bypass (called GOC bypass), characterized by no reducing equivalents being produced during a complete oxidation of glycolate into CO2 catalyzed by three rice-self-originating enzymes, i.e., glycolate oxidase, oxalate oxidase, and catalase. We successfully established this bypass in rice chloroplasts using a multi-gene assembly and transformation system. Transgenic rice plants carrying GOC bypass (GOC plants) showed significant increases in photosynthesis efficiency, biomass yield, and nitrogen content, as well as several other CO2-enriched phenotypes under both greenhouse and field conditions. Grain yield of GOC plants varied depending on seeding season and was increased significantly in the spring. We further demonstrated that GOC plants had significant advantages under high-light conditions and that the improvements in GOC plants resulted primarily from a photosynthetic CO2-concentrating effect rather than from improved energy balance. Taken together, our results reveal that engineering a newly designed chloroplastic photorespiratory bypass could increase photosynthetic efficiency and yield of rice plants grown in field conditions, particularly under high light.
Assuntos
Cloroplastos/metabolismo , Cloroplastos/efeitos da radiação , Engenharia Genética , Luz , Oryza/citologia , Oryza/genética , Fotossíntese/genética , Dióxido de Carbono/metabolismo , Respiração Celular/genética , Respiração Celular/efeitos da radiação , Metabolismo Energético/genética , Metabolismo Energético/efeitos da radiação , Oryza/metabolismo , Oryza/efeitos da radiação , Fenótipo , Fotossíntese/efeitos da radiação , Plantas Geneticamente ModificadasRESUMO
Instructor Talk-noncontent language used by instructors in classrooms-is a recently defined and promising variable for better understanding classroom dynamics. Having previously characterized the Instructor Talk framework within the context of a single course, we present here our results surrounding the applicability of the Instructor Talk framework to noncontent language used by instructors in novel course contexts. We analyzed Instructor Talk in eight additional biology courses in their entirety and in 61 biology courses using an emergent sampling strategy. We observed widespread use of Instructor Talk with variation in the amount and category type used. The vast majority of Instructor Talk could be characterized using the originally published Instructor Talk framework, suggesting the robustness of this framework. Additionally, a new form of Instructor Talk-Negatively Phrased Instructor Talk, language that may discourage students or distract from the learning process-was detected in these novel course contexts. Finally, the emergent sampling strategy described here may allow investigation of Instructor Talk in even larger numbers of courses across institutions and disciplines. Given its widespread use, potential influence on students in learning environments, and ability to be sampled, Instructor Talk may be a key variable to consider in future research on teaching and learning in higher education.
Assuntos
Biologia/educação , Docentes , Ensino , Currículo , Coleta de Dados , Humanos , Aprendizagem , EstudantesRESUMO
The active form of vitamin B6, pyridoxal 5'-phosphate (PLP), plays an essential role in the catalytic mechanism of various proteins, including human glutamate-oxaloacetate transaminase (hGOT1), an important enzyme in amino acid metabolism. A recent molecular and genetic study showed that the E266K, R267H, and P300L substitutions in aspartate aminotransferase, the Arabidopsis analog of hGOT1, genetically suppress a developmentally arrested Arabidopsis RUS mutant. Furthermore, CD analyses suggested that the variants exist as apo proteins and implicated a possible role of PLP in the regulation of PLP homeostasis and metabolic pathways. In this work, we assessed the stability of PLP bound to hGOT1 for the three variant and wildtype (WT) proteins using a combined 6 µs of molecular dynamics (MD) simulation. For the variants and WT in the holo form, the MD simulations reproduced the "closed-open" transition needed for substrate binding. This conformational transition was associated with the rearrangement of the P15-R32 small domain loop providing substrate access to the R387/R293 binding motif. We also showed that formation of the dimer interface is essential for PLP affinity to the active site. The position of PLP in the WT binding site was stabilized by a unique hydrogen bond network of the phosphate binding cup, which placed the cofactor for formation of the covalent Schiff base linkage with K259 for catalysis. The amino acid substitutions at positions 266, 267, and 300 reduced the structural correlation between PLP and the protein active site and/or integrity of the dimer interface. Principal component analysis and energy decomposition clearly suggested dimer misalignment and dissociation for the three variants tested in our work. The low affinity of PLP in the hGOT1 variants observed in our computational work provided structural rationale for the possible role of vitamin B6 in regulating metabolic pathways.
Assuntos
Aspartato Aminotransferase Citoplasmática/genética , Aspartato Aminotransferase Citoplasmática/fisiologia , Fosfato de Piridoxal/metabolismo , Substituição de Aminoácidos/genética , Aspartato Aminotransferase Citoplasmática/ultraestrutura , Aspartato Aminotransferases/metabolismo , Sítios de Ligação/genética , Catálise , Domínio Catalítico , Simulação por Computador , Dimerização , Glutamatos/genética , Glutamatos/fisiologia , Humanos , Modelos Moleculares , Simulação de Dinâmica Molecular , Oxaloacetatos/metabolismo , Análise de Componente Principal , Domínios Proteicos/genética , Fosfato de Piridoxal/química , Fosfato de Piridoxal/fisiologia , Vitamina B 6/metabolismoRESUMO
Many efforts to improve science teaching in higher education focus on a few faculty members at an institution at a time, with limited published evidence on attempts to engage faculty across entire departments. We created a long-term, department-wide collaborative professional development program, Biology Faculty Explorations in Scientific Teaching (Biology FEST). Across 3 years of Biology FEST, 89% of the department's faculty completed a weeklong scientific teaching institute, and 83% of eligible instructors participated in additional semester-long follow-up programs. A semester after institute completion, the majority of Biology FEST alumni reported adding active learning to their courses. These instructor self-reports were corroborated by audio analysis of classroom noise and surveys of students in biology courses on the frequency of active-learning techniques used in classes taught by Biology FEST alumni and nonalumni. Three years after Biology FEST launched, faculty participants overwhelmingly reported that their teaching was positively affected. Unexpectedly, most respondents also believed that they had improved relationships with departmental colleagues and felt a greater sense of belonging to the department. Overall, our results indicate that biology department-wide collaborative efforts to develop scientific teaching skills can indeed attract large numbers of faculty, spark widespread change in teaching practices, and improve departmental relations.
Assuntos
Biologia/educação , Desenvolvimento de Programas , Ensino , Docentes , Objetivos , Humanos , Motivação , Aprendizagem Baseada em Problemas , Estudantes , Inquéritos e QuestionáriosRESUMO
Various chloroplast transit peptides (CTP) have been used to successfully target some foreign proteins into chloroplasts, but for other proteins these same CTPs have reduced localization efficiencies or fail completely. The underlying cause of the failures remains an open question, and more effective CTPs are needed. In this study, we initially observed that two E.coli enzymes, EcTSR and EcGCL, failed to be targeted into rice chloroplasts by the commonly-used rice rbcS transit peptide (rCTP) and were subsequently degraded. Further analyses revealed that the N-terminal unfolded region of cargo proteins is critical for their localization capability, and that a length of about 20 amino acids is required to attain the maximum localization efficiency. We considered that the unfolded region may alleviate the steric hindrance produced by the cargo protein, by functioning as a spacer to which cytosolic translocators can bind. Based on this inference, an optimized CTP, named RC2, was constructed. Analyses showed that RC2 can more effectively target diverse proteins, including EcTSR and EcGCL, into rice chloroplasts. Collectively, our results provide further insight into the mechanism of CTP-mediated chloroplastic localization, and more importantly, RC2 can be widely applied in future chloroplastic metabolic engineering, particularly for crop plants.
Assuntos
Proteínas de Cloroplastos/metabolismo , Oryza/metabolismo , Sequência de Aminoácidos , Proteínas de Escherichia coli/química , Proteínas de Escherichia coli/metabolismo , Oryza/genética , Plantas Geneticamente Modificadas , Dobramento de Proteína , Sinais Direcionadores de Proteínas , Estrutura Terciária de Proteína , Transporte Proteico , Proteólise , Protoplastos/metabolismo , Proteínas Recombinantes de Fusão/metabolismo , Ribulose-Bifosfato Carboxilase/metabolismo , Frações Subcelulares/metabolismoRESUMO
Rapid and dynamic change in hydrogen peroxide (H2O2) levels can serve as an important signal to regulate various biological processes in plants. The change is realized by tilting the balance between its production and scavenging rates, in which membrane-associated NADPH oxidases are known to play a crucial role. Functioning independently from NADPH oxidases, glycolate oxidase (GLO) was recently demonstrated as an alternative source for H2O2 production during both gene-for-gene and non-host resistance in plants. In this study, we show that GLO physically interacts with catalase (CAT) in rice leaves, and that the interaction can be deregulated by salicylic acid (SA). Furthermore, the GLO-mediated H2O2 accumulation is synergistically enhanced by SA. Based on the well-known mechanism of substrate channeling in enzyme complexes, SA-induced H2O2 accumulation likely results from SA-induced GLO-CAT dissociation. In the GLO-CAT complex, GLO-mediated H2O2 production during photorespiration is very high, whereas the affinity of CAT for H2O2 (measured Km ≈ 43 mM) is extraordinarily low. This unique combination can further potentiate the increase in H2O2 when GLO is dissociated from CAT. Taken together, we propose that the physical association-dissociation of GLO and CAT, in response to environmental stress or stimuli, seems to serve as a specific mechanism to modulate H2O2 levels in rice.
Assuntos
Oxirredutases do Álcool/metabolismo , Catalase/metabolismo , Peróxido de Hidrogênio/metabolismo , Oryza/metabolismo , Oryza/efeitos dos fármacos , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/metabolismo , Ácido Salicílico/farmacologiaRESUMO
Vitamin B6 (vitB6) serves as an essential cofactor for more than 140 enzymes. Pyridoxal 5'-phosphate (PLP), active cofactor form of vitB6, can be photolytically destroyed by trace amounts of ultraviolet-B (UV-B). How sun-exposed organisms cope with PLP photosensitivity and modulate vitB6 homeostasis is currently unknown. We previously reported on two Arabidopsis mutants, rus1 and rus2, that are hypersensitive to trace amounts of UV-B light. We performed mutagenesis screens for second-site suppressors of the rus mutant phenotype and identified mutations in the ASPARTATE AMINOTRANSFERASE2 (ASP2) gene. ASP2 encodes for cytosolic aspartate aminotransferase (AAT), a PLP-dependent enzyme that plays a key role in carbon and nitrogen metabolism. Genetic analyses have shown that specific amino acid substitutions in ASP2 override the phenotypes of rus1 and rus2 single mutants as well as rus1 rus2 double mutant. These substitutions, all shown to reside at specific positions in the PLP-binding pocket, resulted in no PLP binding. Additional asp2 mutants that abolish AAT enzymatic activity, but which alter amino acids outside of the PLP-binding pocket, fail to suppress the rus phenotype. Furthermore, exogenously adding vitB6 in growth media can rescue both rus1 and rus2. Our data suggest that AAT plays a role in vitB6 homeostasis in Arabidopsis.
Assuntos
Proteínas de Arabidopsis/genética , Arabidopsis/enzimologia , Aspartato Aminotransferases/genética , Supressão Genética , Vitamina B 6/metabolismo , Sequência de Aminoácidos , Substituição de Aminoácidos , Arabidopsis/genética , Arabidopsis/metabolismo , Arabidopsis/efeitos da radiação , Proteínas de Arabidopsis/metabolismo , Aspartato Aminotransferases/química , Aspartato Aminotransferases/metabolismo , Dados de Sequência Molecular , Alinhamento de Sequência , Raios UltravioletaRESUMO
NITRIC OXIDE-ASSOCIATED1 (NOA1) encodes a circularly permuted GTPase (cGTPase) known to be essential for ribosome assembly in plants. While the reduced chlorophyll and Rubisco phenotypes were formerly noticed in both NOA1-suppressed rice and Arabidopsis, a detailed insight is still necessary. In this study, by using RNAi transgenic rice, we further demonstrate that NOA1 functions in a temperature-dependent manner to regulate chlorophyll and Rubisco levels. When plants were grown at 30°C, the chlorophyll and Rubisco levels in OsNOA1-silenced plants were only slightly lower than those in WT. However, at 22°C, the silenced plants accumulated far less chlorophyll and Rubisco than WT. It was further revealed that the regulation of chlorophyll and Rubisco occurs at the anabolic level. Etiolated WT seedlings restored chlorophyll and Rubisco accumulations readily once returned to light, at either 30°C or 15°C. Etiolated OsNOA1-silenced plants accumulated chlorophyll and Rubisco to normal levels only at 30°C, and lost this ability at low temperature. On the other hand, de-etiolated OsNOA1-silenced seedlings maintained similar levels of chlorophyll and Rubisco as WT, even after being shifted to 15°C for various times. Further expression analyses identified several candidate genes, including OsPorA (NADPH: protochlorophyllide oxidoreductase A), OsrbcL (Rubisco large subunit), OsRALyase (Ribosomal RNA apurinic site specific lyase) and OsPuf4 (RNA-binding protein of the Puf family), which may be involved in OsNOA1-regulated chlorophyll biosynthesis and Rubisco formation. Overall, our results suggest OsNOA1 functions in a temperature-dependent manner to regulate chlorophyll biosynthesis, Rubisco formation and plastid development in rice.
Assuntos
Clorofila/biossíntese , Oryza/metabolismo , Proteínas de Plantas/fisiologia , Ribulose-Bifosfato Carboxilase/biossíntese , Temperatura , Sequência de Bases , Primers do DNA , Inativação Gênica , Análise de Sequência com Séries de Oligonucleotídeos , Oryza/enzimologia , Proteínas de Plantas/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transcrição GênicaRESUMO
Ultraviolet B light (UV-B; 280-320 nm) perception and signaling are well-known phenomena in plants, although no specific UV-B photoreceptors have yet been identified. We previously reported on the root UV-B sensitive1 (rus1) mutants in Arabidopsis (Arabidopsis thaliana), which display a block to development under very-low-fluence-rate UV-B (<0.1 mumol m(-2) s(-1)) after the seedling emerges from the seed. Here, we report the analysis and cloning of the rus2-1 mutation in Arabidopsis. The phenotype of rus2-1 mutant seedlings is virtually indistinguishable from the phenotype of rus1 seedlings. A map-based approach was used to clone RUS2. RUS2 encodes a domain of unknown function (DUF647)-containing protein that is homologous to the RUS1 protein. rus1-2 rus2-1 double mutant seedlings have the same phenotype as both rus1 and rus2 single mutants, suggesting that the two genes work in the same pathway. RUS2-Green Fluorescent Protein shows a similar expression pattern as that of RUS1-Green Fluorescent Protein, and RUS1 and RUS2 proteins interact physically in yeast. This protein-protein interaction depends on the DUF647 domain, and site-directed mutagenesis identified specific residues in DUF647 that are required for both protein-protein interaction and physiological function. Six RUS genes are found in Arabidopsis, rice (Oryza sativa), and moss (Physcomitrella patens), and one RUS member, RUS3, is conserved in plants and animals. Our results demonstrate that RUS2 works with RUS1 in a root UV-B-sensing pathway that plays a vital role in Arabidopsis early seedling morphogenesis and development.
Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Arabidopsis/efeitos da radiação , Raízes de Plantas/efeitos da radiação , Transdução de Sinais/efeitos da radiação , Raios Ultravioleta , Sequência de Aminoácidos , Animais , Arabidopsis/crescimento & desenvolvimento , Proteínas de Arabidopsis/química , Sequência de Bases , Mapeamento Cromossômico , Clonagem Molecular , Teste de Complementação Genética , Meristema/citologia , Meristema/metabolismo , Meristema/efeitos da radiação , Modelos Biológicos , Dados de Sequência Molecular , Mutação/genética , Fenótipo , Filogenia , Raízes de Plantas/citologia , Raízes de Plantas/crescimento & desenvolvimento , Ligação Proteica/efeitos da radiação , Estrutura Terciária de Proteína , Saccharomyces cerevisiae/metabolismo , Plântula/crescimento & desenvolvimento , Plântula/efeitos da radiação , Relação Estrutura-AtividadeRESUMO
Cellular oxalate, widely distributed in many plants, is implicated to play important roles in various functions and is also known to affect food qualities adversely in fruits and vegetables. How oxalate is regulated in plants is currently not well understood. Glycolate oxidase (GLO) has long been considered as an important player in oxalate accumulation in plants. To gain further insight into the biochemical and molecular mechanisms, the possible roles of GLO in the process were studied. Drastically different levels of oxalate could be achieved by treating rice with various nitrogen forms (nitrate versus ammonium). While nitrate stimulated oxalate accumulation, ammonium reduced its level. Such treatments resulted in similar pattern changes for some other related organic acids, such as glycolate, oxaloacetate, and malate. By feeding plants with exogenous glycolate it was possible almost completely to restore the ammonium-decreased oxalate level. Under the two treatments few differences were observed for GLO mRNA levels, protein levels, and in vitro activities. Both K(m) for glycolate/glyoxylate and K(i) for oxalate remained almost the same for GLO purified from either nitrate- or ammonium-fed leaves. A further in vivo study, with transgenic plants carrying an estradiol-inducible GLO antisense gene, showed that, while the estradiol-induced antisense expression remarkably reduced both GLO protein levels and activities, oxalate levels were not significantly altered in the estradiol-treated transgenic plants. Taken together, it is suggested that oxalate accumulation and regulation is independent of GLO in rice leaves.
Assuntos
Oxirredutases do Álcool/metabolismo , Oryza/metabolismo , Oxalatos/metabolismo , Folhas de Planta/metabolismo , Catálise , DNA Antissenso , DNA de Plantas , Expressão Gênica , Cinética , Nitrogênio/fisiologia , Oryza/genética , Plantas Geneticamente Modificadas/metabolismoRESUMO
The cell wall-associated receptor kinase (WAK) and WAK-like kinase (WAKL) gene family members are good candidates for physical linkers that signal between the cell wall and the cytoplasmic compartment. Previous studies have suggested that while some WAK/WAKL members play a role in bacterial pathogen and heavy-metal aluminum responses, others are involved in cell elongation and plant development. Here, we report a functional role for the WAKL4 gene in Arabidopsis (Arabidopsis thaliana) mineral responses. Confocal microscopic studies localized WAKL4-green fluorescent protein fusion proteins on the cell surfaces suggesting that, like other WAK/WAKL proteins, WAKL4 protein is associated with the cell wall. Histochemical analyses of the WAKL4 promoter fused with the -glucuronidase reporter gene have shown that WAKL4 expression is induced by Na+, K+, Cu2+, Ni2+, and Zn2+. A transgenic line with a T-DNA insertion at 40-bp upstream of the WAKL4 start codon was characterized. While the T-DNA insertion had little effect on the WAKL4 transcript levels under normal growth conditions, it significantly altered the expression patterns of WAKL4 under various conditions of mineral nutrients. Semiquantitative and quantitative reverse transcription-PCR analyses showed that the promoter impairment abolished WAKL4-induced expression by Na+, K+, Cu2+, and Zn2+, but not by Ni2+. Whereas the WAKL4 promoter impairment resulted in hypersensitivity to K+, Na+, Cu2+, and Zn2+, it conferred a better tolerance to toxic levels of the Ni2+ heavy metal. WAKL4 was required for the up-regulation of zinc transporter genes during zinc deficiency, and the WAKL4 T-DNA insertion resulted in a reduction of Zn2+ accumulation in shoots. A WAKL4-green fluorescent protein fusion gene driven by either the WAKL4 native promoter or the 35S constitutive promoter complemented the phenotypes. Our results suggest versatile roles for WAKL4 in Arabidopsis mineral nutrition responses.
Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/enzimologia , Proteínas de Membrana/metabolismo , Proteínas Quinases/metabolismo , Arabidopsis/efeitos dos fármacos , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Sequência de Bases , Parede Celular/enzimologia , DNA de Plantas/genética , Genes de Plantas , Proteínas de Membrana/genética , Minerais/farmacologia , Mutagênese Insercional , Níquel/farmacologia , Plantas Geneticamente Modificadas , Regiões Promotoras Genéticas , Proteínas Quinases/genéticaRESUMO
The wall-associated kinase (WAK) gene family, one of the receptor-like kinase (RLK) gene families in plants, plays important roles in cell expansion, pathogen resistance, and heavy-metal stress tolerance in Arabidopsis (Arabidopsis thaliana). Through a reiterative database search and manual reannotation, we identified 125 OsWAK gene family members from rice (Oryza sativa) japonica cv Nipponbare; 37 (approximately 30%) OsWAKs were corrected/reannotated from earlier automated annotations. Of the 125 OsWAKs, 67 are receptor-like kinases, 28 receptor-like cytoplasmic kinases, 13 receptor-like proteins, 12 short genes, and five pseudogenes. The two-intron gene structure of the Arabidopsis WAK/WAK-Likes is generally conserved in OsWAKs; however, extra/missed introns were observed in some OsWAKs either in extracellular regions or in protein kinase domains. In addition to the 38 OsWAKs with full-length cDNA sequences and the 11 with rice expressed sequence tag sequences, gene expression analyses, using tiling-microarray analysis of the 20 OsWAKs on chromosome 10 and reverse transcription-PCR analysis for five OsWAKs, indicate that the majority of identified OsWAKs are likely expressed in rice. Phylogenetic analyses of OsWAKs, Arabidopsis WAK/WAK-Likes, and barley (Hordeum vulgare) HvWAKs show that the OsWAK gene family expanded in the rice genome due to lineage-specific expansion of the family in monocots. Localized gene duplications appear to be the primary genetic event in OsWAK gene family expansion and the 125 OsWAKs, present on all 12 chromosomes, are mostly clustered.
Assuntos
Parede Celular/enzimologia , Regulação da Expressão Gênica de Plantas , Genes de Plantas/genética , Família Multigênica/genética , Oryza/enzimologia , Oryza/genética , Fosfotransferases/genética , Arabidopsis/genética , Cromossomos de Plantas , Biologia Computacional , Duplicação Gênica , Genoma de Planta , Hordeum/genética , Íntrons/genética , Análise em Microsséries , Oryza/citologia , Fosfotransferases/química , Fosfotransferases/classificação , Filogenia , Mapeamento Físico do Cromossomo , Proteínas de Plantas/química , Proteínas de Plantas/genética , Pseudogenes/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismoRESUMO
The Arabidopsis cell wall-associated kinase (WAK) and WAK-like kinase (WAKL) family of receptor-like kinase genes encodes transmembrane proteins with a cytoplasmic serine/threonine kinase domain and an extracellular region containing epidermal growth factor-like repeats. Previous studies have suggested that some WAK members are involved in plant defense and heavy metal responses, whereas others are required for cell elongation and plant development. The WAK/WAKL gene family consists of 26 members in Arabidopsis and can be divided into four groups. Here, we describe the characterization of group 2 members that are composed of a cluster of seven tandemly arrayed WAKL genes. The predicted WAKL proteins are highly similar in their cytoplasmic region but are more divergent in their predicted extracellular ligand-binding region. WAKL7 encodes a truncated WAKL isoform that is predicted to be secreted from the cytoplasm. Ratios of nonsynonymous to synonymous substitutions suggest that the extracellular region is subject to diversifying selection. Comparison of the WAKL and WAK gene clusters suggests that they arose independently. Protein gel-blot and immunolocalization analyses suggest that WAKL6 is associated with the cell wall. Histochemical analyses of WAKL promoters fused with the beta-glucuronidase reporter gene have shown that the expressions of WAKL members are developmentally regulated and tissue specific. Unlike WAK members whose expressions were found predominately in green tissues, WAKL genes are highly expressed in roots and flowers. The expression of WAKL5 and WAKL7 can be induced by wounding stress and by the salicylic acid analog 2,6-dichloroisonicotinic acid in an nonexpressor of pathogenesis-related gene 1-dependent manner, suggesting that they, like some WAK members, are wound inducible and can be defined as pathogenesis-related genes.
Assuntos
Arabidopsis/genética , Parede Celular/enzimologia , Proteínas de Membrana/genética , Proteínas Quinases/genética , Sequência de Aminoácidos , Arabidopsis/enzimologia , Proteínas de Arabidopsis/genética , Sequência de Bases , Parede Celular/genética , Primers do DNA , Meio Ambiente , Regulação da Expressão Gênica no Desenvolvimento/genética , Regulação Enzimológica da Expressão Gênica/genética , Regulação da Expressão Gênica de Plantas/genética , Genoma de Planta , Isoenzimas/química , Isoenzimas/genética , Proteínas de Membrana/química , Dados de Sequência Molecular , Família Multigênica , Regiões Promotoras Genéticas/genética , Proteínas Quinases/química , Alinhamento de SequênciaRESUMO
Here, we report the aluminum (Al)-induced organ-specific expression of a WAK1 (cell wall-associated receptor kinase 1) gene and cell type-specific localization of WAK proteins in Arabidopsis. WAK1-specific reverse transcriptase-polymerase chain reaction analysis revealed an Al-induced WAK1 gene expression in roots. Short- and long-term analysis of gene expression in root fractions showed a typical "on" and "off" pattern with a first peak at 3 h of Al exposure followed by a sharp decline at 6 h and a complete disappearance after 9 h of Al exposure, suggesting the WAK1 is a further representative of Al-induced early genes. In shoots, upon root Al exposure, an increased but stable WAK1 expression was observed. Using confocal microscopy, we visualized Al-induced closure of leaf stomata, consistent with previous suggestions that the Al stress primarily experienced in roots associated with the transfer of root-shoot signals. Elevated levels of WAK protein in root cells were observed through western blots after 6 h of Al exposure, indicating a lag time between the Al-induced WAK transcription and translation. WAK proteins are localized abundantly to peripheries of cortex cells within the elongation zone of the root apex. In these root cells, disintegration of cortical microtubules was observed after Al treatment but not after the Al analog lanthanum treatments. Tip-growing control root hairs, stem stomata, and leaf stomatal pores are characterized with high amounts of WAKs, suggesting WAKs are accumulating at plasma membrane domains, which suffer from mechanical stress and lack dense arrays of supporting cortical microtubules. Further, transgenic plants overexpressing WAK1 showed an enhanced Al tolerance in terms of root growth when compared with the wild-type plants, making the WAK1 one of the important candidates for plant defense against Al toxicity.