Full-length Sequencing and Genotyping of Hepatitis A Virus among Acute Hepatic Patients in Iran.

BACKGROUND: Given the lack of access to a full-length sequence of hepatitis A virus (HAV) and scarce information about its circulating genotype, sub-type and strain in Iran, two specimens were isolated from two patients with clinical symptoms of acute HAV to determine the full-length sequence of HAV. Following the phylogenetic and molecular study, we determined HAV genotype, sub-genotype, and strain of circulating virus in Iran. METHODS: According to real-time PCR results, 16 pairs of overlapped specific primers were used to determine the full-length sequence of HAV by whole-genome amplification (WGA) and using the Sanger method. Moreover, the results were assessed using Chromas, CLC Genomics Workbench, Mega 6, and RDP software. RESULTS: The full-length genome of HAV was amplified and sequenced with a length of 7,182 nucleotides. According to the obtained sequences, the phylogenetic tree of the mentioned viruses was drawn using MEGA 6 software and 44 full-genome viruses registered in the GenBank worldwide. Afterwards, the same process was repeated based on the protein sequence of VP1-P2A fragment in Iranian samples along with the other 22 registered protein sequences of GenBank to confirm the results of the full-genome phylogenetic tree. CONCLUSIONS: In this study, complete sequencing of two HAV specimens was carried out using the overlapping amplification and Sanger methods. According to the results of the phylogenetic tree, the circulating HAV in Iran had Genotype I and sub-genotype B and strain HM-175. In the present study, the full sequences of HAV of the two specimens were registered with accession numbers of BankIt 2277890/MN746031 and BankIt 2287607/MN746032.

Intralesional gene expression profile of JAK-STAT signaling pathway and associated cytokines in Leishmania tropica-infected patients.

Background: The JAK-STAT signaling pathway is a central cascade of signal transduction for the myriad of cytokines in which dysregulation has been implicated in progression of inflammatory and infectious diseases. However, the involvement of this pathway in human cutaneous leishmaniasis (CL) due to Leishmania (L.) tropica warrants further investigation. Methods: This study sought to investigate differential gene expression of several cytokines and their associated jak-stat genes in the lesions of L. tropica-infected patients byquantitative Real-Time PCR. Further, the expression of five inhibitory immune checkpoint genes was evaluated. Results: Results showed that the gene expression levelsof both Th1 (ifng, il12, il23) and Th2 (il4, il10) types cytokines were increased in the lesion of studied patients. Further, elevated expression levels of il35, il21, il27 and il24 genes were detected in the lesions of CL patients. Notably, the expression of the majority of genes involved in JAK/STAT signaling pathway as well as checkpoint genes including pdl1, ctla4 and their corresponding receptors was increased. Conclusion: Our finding revealed dysregulation of cytokines and related jak-stat genes in the lesion of CL patients. These results highlight the need for further exploration of the functional importance of these genes in the pathogenesis of, and immunity to, CL.

Increase resistant rates and ESBL production between E. coli isolates causing urinary tract infection in young patients from Iran.

Emerging antimicrobial resistance rates and Extended-spectrum beta-lactamase producing Escherichia coli recovered from urinary tract infections (UTI) is an increasing problem in specific regions, limiting therapeutic options. One hundred E. coli isolates causing UTI in patients with age from 2 months to 12 years admitted at CMC in the period of April 2009 to March 2010 were tested for antibiotic susceptibility using the disk diffusion method. Surprisingly high resistance rates were recorded for E. coli against TMP/SMX (84%), cefalotin (66%), cefuroxime (50%), cefixime (50%) and ceftriaxone (45%). Antimicrobial susceptibility of E. coli isolates was followed by meropenem (98%), amikacin (95%), nitrofurantoin (91%) and gentamicin (68%). Extended spectrum beta-lactamase production, was observed in 32% of community and 42% of nosocomial isolates. The results of this study and numerous observations regarding the increasing resistance to these antibiotics, in several countries, emphasize the need for local population-specific surveillance for guiding empirical therapy for UTI in children.

Investigating the Effects of Cement and Polymer Grouting on the Shear Behavior of Rock Joints.

This study carried out a comparison between cement grouting and chemical grouting, using epoxy and polyurethane, with respect to their effects on the shear behavior of joints. Joint replicas, with three different grades of surface roughness, were molded and grouted by means of cement and epoxy grouts of various mixtures. To investigate their shear behavior, samples were subjected to direct shear tests under constant normal load (CNL) condition. According to the results obtained, grouting improves the overall shear strength of the rock joints. All the grouted samples yielded higher maximum and residual shear strength in comparison with the non-grouted joint. Grouting resulted in an improvement in the cohesion of all the samples. However, a fall in friction angle by 5.26° in the sample with JRC of nine was observed, yet it was reduced by 2.36° and 3.26° for joints with JRC of 14 and 19, respectively. Cement grouts were found to have a more brittle behavior, whereas the chemical grouts were more ductile. Higher amounts of cement used in the grout mixture do not provide as much cohesion and only increase the brittleness of the grout. As a result of being more brittle, cement grout breaks into small pieces and joint planes are in better contact during shearing; consequently, there would be less of a fall in friction angle as opposed to epoxy grout whose ductile characteristic prevents grout chipping; therefore, joint planes are not in contact and a greater fall in the friction angle occurs. There was no noticeable change in the cohesion of the larger grouted joints. However, the friction angle of both natural and grouted joints increased in the larger joint. This can be related to the distribution of random peaks and valleys on the joint surface, which increases with the joint size.

Risk factors of congenital heart diseases: A hospital-based case-control study in Isfahan, Iran.

BACKGROUND: Improving knowledge towards risk factors for congenital heart disease (CHD) is important because of its high mortality and morbidity and trying for prevention of occurrence of CHD. METHODS: This case-control study was conducted on a total of 898 children with their mothers, who referred to the Clinic of Pediatric Cardiology of School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran, during the years of 2014 to 2016. Cases comprised of 464 children with CHD diagnosed by echocardiography and controls were 434 sex- and age-matched children without any evidence of CHD, who were admitted for a heart check-up at the same study period and in similar conditions. The children's parents completed check lists for collecting demographic characteristics, family history of CHD, history of obesity in mother, history of abortion and diseases in mother, use of medicine during pregnancy, exposure to teratogens during pregnancy, and children characteristics such as birth height and birth weight, etc. RESULTS: Based on the results of data analyses with multiple logistic regression model [odds ratio (OR) with 95% confidence interval (CI)], history of obesity in mother before pregnancy, history of abortion, parental consanguinity, exposure to cigarette smoke during pregnancy, exposures to teratogens in the first trimester of the pregnancy, and use of medicine during pregnancy were associated with an increased odds of CHDs. CONCLUSION: Results of this study emphasizes the use of policies that enhance pre-marital counseling, regular counseling during pregnancy, treatment of mothers' disease, and enhancing knowledge of women of childbearing age about exposure to certain teratogens for controlling risk factors of CHD.

The effects of aerobic training, resistance training, combined training, and healthy eating recommendations on lipid profile and body mass index in overweight and obese children and adolescents: A randomized clinical trial.

BACKGROUND: The aim of this study was to evaluate the effects of 8 weeks of aerobic training, resistance training (RT), combined training, and nutritional recommendations on lipid profile and body mass index (BMI) in obese and overweight children and adolescents. METHODS: This randomized, clinical trial was conducted on 120 children and adolescents (10-19 years of age) with overweight and obesity. Participants were divided into 4 groups, the 3 intervention groups of high-intensity interval training (HIIT), RT, and combined training, and 1 non-exercising control group with healthy eating recommendations. We considered 24 sessions of training during 8 weeks for the intervention groups. The participants' anthropometric indices and lipid profile were assessed before and after the intervention. RESULTS: There were no significant differences between the groups in terms of anthropometric indices and lipid profiles before the intervention. After the intervention, there was a significant difference between the groups in terms of high-density lipoprotein (HDL) level; the control group (37.70 ± 9.45) and the HIIT group (43.65 ± 9.09) displayed the lowest and highest mean, respectively (P = 0.040). Comparison of physical variables and blood lipid profiles before and after the intervention showed a significant difference in waist circumference (P = 0.030), hip circumference (P ≤ 0.001), and HDL level (P = 0.040) in RT, HIIT, combined, and control groups. CONCLUSION: These results demonstrate that the combined training program and HIIT program with nutritional recommendations in overweight and obese children and adolescents were more effective in reducing BMI and hip circumference, respectively.

Increase resistant rates and ESBL production between E. coli isolates causing urinary tract infection in young patients from Iran

Emerging antimicrobial resistance rates and Extended-spectrum beta-lactamase producing Escherichia coli recovered from urinary tract infections (UTI) is an increasing problem in specific regions, limiting therapeutic options. One hundred E. coli isolates causing UTI in patients with age from 2 months to 12 years admitted at CMC in the period of April 2009 to March 2010 were tested for antibiotic susceptibility using the disk diffusion method. Surprisingly high resistance rates were recorded for E. coli against TMP/SMX (84%), cefalotin (66%), cefuroxime (50%), cefixime (50%) and ceftriaxone (45%). Antimicrobial susceptibility of E. coli isolates was followed by meropenem (98%), amikacin (95%), nitrofurantoin (91%) and gentamicin (68%). Extended spectrum beta-lactamase production, was observed in 32% of community and 42% of nosocomial isolates. The results of this study and numerous observations regarding the increasing resistance to these antibiotics, in several countries, emphasize the need for local population-specific surveillance for guiding empirical therapy for UTI in children.