Requirement of the exopolyphosphatase gene for cellular acclimation to phosphorus starvation in a cyanobacterium, Synechocystis sp. PCC 6803.

Polyphosphate, which is ubiquitous in cells in nature, is involved in a myriad of cellular functions, and has been recently focused on its metabolism related with microbial acclimation to phosphorus-source fluctuation. In view of the ecological importance of cyanobacteria as the primary producers, this study investigated the responsibility of polyphosphate metabolism for cellular acclimation to phosphorus starvation in a cyanobacterium, Synechocystis sp. PCC 6803, with the use of a disruptant (Δppx) as to the gene of exopolyphosphatase that is responsible for polyphosphate degradation. Δppx was similar to the wild type in the cellular content of polyphosphate to show no defect in cell growth under phosphorus-replete conditions. However, under phosphorus-starved conditions, Δppx cells were defective in a phosphorus-starvation dependent decrease of polyphosphate to show deleterious phenotypes as to their survival and the stabilization of the photosystem complexes. These results demonstrated some crucial role of exopolyphosphatase to degrade polyP in the acclimation of cyanobacterial cells to phosphorus-starved conditions. Besides, it was found that ppx expression is induced in Synechocystis cells in response to phosphorus starvation through the action of the two-component system, SphS and SphR, in the phosphate regulon. The information will be a foundation for a fuller understanding of the process of cyanobacterial acclimation to phosphorus fluctuation.

Preferential phosphatidylglycerol synthesis via phosphorus supply through rRNA degradation in the cyanobacterium, Synechocystis sp. PCC 6803, under phosphate-starved conditions.

Photosynthetic organisms often encounter phosphorus (P) limitation in natural habitats. When faced with P limitation, seed plants degrade nucleic acids and extra-plastid phospholipids to remobilize P, thereby enhancing their internal-P utilization efficiency. Although prokaryotic and eukaryotic photosynthetic organisms decrease the content of phosphatidylglycerol (PG) under P-limited conditions, it remains unclear whether PG is degraded for P remobilization. Moreover, information is limited on internal-P remobilization in photosynthetic microbes. This study investigates internal-P remobilization under P-starvation (-P) conditions in a cyanobacterium, Synechocystis sp. PCC 6803, focusing on PG and nucleic acids. Our results reveal that the PG content increases by more than double in the -P culture, indicating preferential PG synthesis among cellular P compounds. Simultaneously, the faster increases of glycolipids counteract this PG increase, which decreases the PG proportion in total lipids. Two genes, glpD and plsX, contribute to the synthesis of diacylglycerol moieties in glycerolipids, with glpD also responsible for the polar head group synthesis in PG. The mRNA levels of both glpD and plsX are upregulated during -P, which would cause the preferential metabolic flow of their P-containing substrates toward glycerolipid synthesis, particularly PG synthesis. Meanwhile, we find that RNA accounts for 62% of cellular P, and that rRNA species, which makes up the majority of RNA, are degraded under -P conditions to less than 30% of their initial levels. These findings emphasize the importance of PG in -P-acclimating cell growth and the role of rRNA as a significant internal-P source for P remobilization, including preferential PG synthesis.