Blood-based assessment of oxidative stress, inflammation, endocrine and metabolic adaptations in eventing horses accounting for plasma volume shift after exercise.

BACKGROUND: After submaximal exercise, blood values of eventing horses show physiological reactions. OBJECTIVES: This prospective longitudinal study investigated blood parameters in 20 elite eventing horses before and after two-four-star cross-country rides. METHODS: Using a mixed model adjusting for plasma volume shift, we assessed exercise-dependent parameters and compared blood values with reference ranges for healthy horses at rest. RESULTS: Following exercise, cortisol, triiodothyronine (T3) and thyroxine (T4) showed short-term increases, and superoxide-dismutase showed a small short-term increase. Hepatic values showed short-term (haemoglobin [HGB], globulins) or sustained increases (bilirubin, glutamate dehydrogenase, alanine aminotransferase). Digestion-related parameters showed small short-term increases (α-amylase, triglycerides) or decreases (cholesterol, DGGR-lipase), apparent through plasma shift adjustment. Zinc decreased in the short term, and iron showed a delayed decrease. White blood cell count increased persistently after training, whereas serum amyloid A remained unchanged. CONCLUSIONS: Exercised eventing horses had consistently elevated HGB and cortisol levels 10 and 30 min after submaximal exercise, exceeding the reference ranges for healthy horses at rest. Exercise activates the hypothalamic-pituitary-adrenocortical and hypothalamic-pituitary-thyroid axes. Antioxidant activity was observed. Increased energy requirements led to the mobilization of energy reserves, and a sustained increase in liver enzymes indicated hepatocellular injury. Mild haemolysis suggested increased muscle metabolism, whereas signs of inflammation were subtle. Further research is needed to identify which horses deviate from mean values.

Blood-Based Markers for Skeletal and Cardiac Muscle Function in Eventing Horses before and after Cross-Country Rides and How They Are Influenced by Plasma Volume Shift.

Horses competing in cross-country tests are subjected to high physical demands. Within the scope of this prospective longitudinal study, blood values of 20 elite eventing horses were examined before and after two- to four-star cross-country rides. The aim was to find out whether blood-based markers for skeletal muscle and cardiac muscle function change after cross-country exercise. Parameters that provide information about fluid balance, muscle enzymes, metabolites and cardiac muscle-specific markers were investigated. We developed an approach to eliminate the concentration changes caused by reduced plasma volume. Parameters were measured pre, 10 and 30 min post exercise and the next morning and were evaluated using a mixed model. Thirty minutes after exercise, most parameter concentrations changed in an exercise-dependent manner. The next morning, most exercise-related markers recovered rapidly, while creatine kinase (CK) (26% increase; p = 0.008) and lactate dehydrogenase (LDH) (15% increase; p < 0.001) showed a declining but sustained increase. Cardiac troponin I (cTnI) increased above the reference range in 40 of the 55 rides (73%) and in 18 of 20 horses in the morning after exercise.

Monitoring Performance in Show Jumping Horses: Validity of Non-specific and Discipline-specific Field Exercise Tests for a Practicable Assessment of Aerobic Performance.

Show jumping is a highly specialized equestrian discipline that requires technical skill but also power and fitness. Monitoring the horses' aerobic performance is therefore essential in order to verify whether the training has induced the desired cardiovascular and muscular adaptations. This study therefore aimed at evaluating the validity of non-specific and discipline-specific field exercise tests for objective evaluation of aerobic performance in show jumpers. For this purpose, data obtained from horses competing at Junior and Young Rider level during show jumping competitions as well as field exercise tests were retrospectively analyzed. The effect of the level of difficulty, the horses' age, the penalty score and the horses' previous level of performance on blood lactate concentrations after show jumping competitions (100 observations in 49 horses) was evaluated by linear mixed effects models (horse as random effect). Estimated marginal means significantly increased from 140 (4.1 mmol/L) to 150 cm (5.2 mmol/L) classes (P = 0.02). Furthermore, post-exercise lactate values significantly increased with the horses' age (P = 0.001). Another group of 12 horses performed a standardized incremental field exercise test on a track (SETtrack), a standardized show jumping course (SETcourse) and a standardized grid exercise (SETgrid) each on three consecutive days. Indices of aerobic performance, derived from the SETtrack [velocity at a heart rate of 140 bpm (V140) and at a lactate concentration of 2 mmol/L (V La2 )] were highly correlated with heart rate (V140: r = -0.75, P = 0.005; V La2 : r =-0.66, P = 0.02) and lactate (V140: r = -0.73, P = 0.02; V La2 : r = -0.72, P = 0.02) in response to SETcourse as well as heart rate during SETgrid (V140: r = -0.73, P = 0.02; V La2 : r = -0.76, P = 0.01). Subjective rating of muscular fatigue was significantly correlated to the mean heart rate during SETcourse (r = -0.64, P = 0.05) and SETgrid (r = -0.74, P = 0.02) but not to the aerobic indices calculated from SETtrack. Besides non-specific incremental field tests, performance monitoring in show jumpers should therefore also include discipline-specific tests that more closely reflect the internal load induced by show jumping competitions.