[The efficacy of sorafenib to prevent relapse in patients with FLT3-ITD mutation positive acute myeloid leukemia after allogeneic hematopoietic stem cell transplantation].

To study the efficacy of sorafenib to prevent relapse in patients with FLT3-ITD mutation positive acute myeloid leukemia (AML) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). A total of 7 cases with FLT3-ITD positive AML have received allo-HSCT in our department from May 2013 to January 2015. Six cases were administrated with sorafenib after hematopoietic reconstruction. Another patient relapsed on day 192 past allo-HSCT, then she started to use sorafenib after remission of re-induction regimens. Five patients survived. The median progression free survival was 280(126-366)day. This study suggests that sorafenib might prevent relapse past allo-HSCT and improve survival in patients with FLT3-ITD positive AML.

[Textual research on the unclear source of citations from Hua Tuo correlation works cited by the Compendium of Materia Medica].

The Compendium of Materia Medica is a magnum opus, in which a large number of literatures were cited. By searching with keywords, there were 30 citations related with Hua Tuo in this book. However, some citations were not specific, even lack of the source information. The objective of this article is to find out the contents, first-hand source and editions of the primary literatures by doing textual research.As a result, the sources of quotations about the Hua Tuo's Prescription for Madam Pengcheng and Hua Tuo's Emergency Cases Prescriptions were discovered.Meanwhile, it was confirmed that Zhongzang Jing (, Classic of the Central Viscera) with 3 volumes and books of prescriptions during Song and Ming Dynasties were cited by the Compendium of Materia Medica. This solve the long term problem about Hua Tuo's citations, and might benefit further study about the citations of the Compendium of Materia Medica.

The effects of dietary supplementation of microencapsulated and the extract of seed on growth performance, immune functions, and serum biochemical parameters in broiler chickens.

This experiment was conducted to investigate the effects of microencapsulted (MEF) and the extract of seed (ECOS) on growth performance, immune functions, and serum biochemical parameters in broiler chickens. A total of 240 1-d-old male broilers were randomly allotted into 6 treatments with 8 replicates/treatment and 5 broilers in each cage. The dietary treatments included 1) a basal diet without antibiotic (group A), 2) the basal diet + 1 g MEF/kg diet (1 × 10 cfu/g MEF; group B), 3) the basal diet + 300 mg ECOS/kg diet (group C), 4) the basal diet + 300 mg ECOS/kg diet + 1 g MEF/kg diet (group D), 5) the basal diet + 500 mg ECOS/kg diet (group E), and 6) the basal diet + 500 mg ECOS/kg diet + 1 g MEF/kg diet (group F). The feeding experiment included 2 phases: the starter phase from Day 1 through 21 and the grower phase from Day 22 through 42. The results showed that a diet supplemented with MEF and ECOS had no significant effect on ADG, ADFI, feed conversion ratio, and average BW during the whole experimental period ( > 0.05), but group F showed an improving trend in growth performance. Serum IL-2, IgA, and IgG levels and spleen index were significantly affected by dietary treatment ( < 0.05). Serum IgA and IgG levels and spleen index in group F were significantly higher than in the group A ( < 0.05), and the IL-2 level was significantly decreased ( < 0.05) on Days 21 and 42. Compared with the group A, diets supplemented with MEF and ECOS can significantly decrease total cholesterol, low-density lipoprotein cholesterol, triglycerides, and blood urea nitrogen levels ( < 0.05) and increase the high-density lipoprotein cholesterol level on Days 21 and 42. Concentrations of serum biochemical parameters were significantly increased in group F ( < 0.05). In summary, the results indicated that dietary supplementation of MEF and/or ECOS had no significant effect on growth performance but significantly increased spleen index and the levels of serum IgA and IgG and improved serum lipid metabolism. The 1 g MEF/kg diet (1 × 10 cfu/g diet) plus 500 mg ECOS/kg diet was the optimum supplemental dose in this experiment.

Primary malignant melanoma of the vagina: a report of seven cases.

Seven patients with primary melanoma of the vagina, five untreated and two recurrent, are reported. The ages ranged from 25-55 years, with an average of 42.3 years. The common symptoms were vaginal bleeding and a vaginal mass, and the most common site was the posterior wall of the vagina (three of seven patients). Two patients survived for more than five years.

[Endodermal sinus tumor of ovary--analysis of 35 cases].

Thirty-five patients with pure endodermal sinus tumor of the ovary treated in our hospital from 1962 to 1984 are reported. There were 8 Stage I, 13 Stage III lesions and 14 with recurrent tumor from other hospitals. All the patients were treated by surgery followed by chemotherapy including single drug or combined chemotherapy. Five of 7 patients on high-dose DDP (120 mg/M2) responded. Two of the Stage I patients survived for 33 and 39 months. One of 2 patients by AcFuCy regime was free of tumor for 82 months. Four of 6 patients with advanced tumor treated by VAC regime responded, but only one was alive without evidence of tumor for 30 months. In 21 patients treated with other combination or single drug chemotherapy before 1980, only one (Stage Ia) is alive. It is indicated that surgery combined with multi-drug chemotherapy is the best management and high-dose DDP, VAC and AcFuCy are effective in treating pure endodermal sinus tumor of the ovary. Monitoring of serum AFP levels of the patients with endodermal sinus tumor can predict the course of the disease.

[Lung metastasis from carcinoma of the uterine cervix following radiotherapy--analysis of 157 cases].

From Mar. 1958 to Apr. 1975, 9,384 patients with carcinoma of the cervix were treated by radiotherapy in our hospital. Up to Apr. 1981, 222 (2.4%) developed lung metastasis. The diagnosis, therapy and survival rate of 157 patients were analysed. It is shown that the lung metastasis which occurs more frequently on the right side is closely related to the stage of the disease. However, there is no difference between the upper and lower lobe in unilateral lung metastasis. The early diagnosis of lung metastasis of carcinoma of the cervix is very important for which close and regular follow up of the patient should be stressed. The treatment depends on the extent of metastasis and condition of the patient. The best treatment for single metastasis is pneumonectomy as well as radiotherapy. Chemotherapy is indicated only for advanced lesions.

[Superficial lymph node metastasis from ovarian cancer--an analysis of 31 cases].

From March 1958 to June 1988, 53 cases of ovarian cancer with superficial lymph node metastasis (SLM) were treated in our hospital comprising 5.6% of patients with malignant ovarian tumors in the same period. Of 53 cases, 31 were proven by histopathology, and 27 had serous adenocarcinoma. Of these 31 cases, 15 with Stage IV lesion had SLM at time when first seen in the clinic and the others developed SLM during treatment or afterwards. The median survival was 34.7, 11.8 and 12.9 months in Grades I, II and III, respectively. Prognosis is related to pathological grade. The median survival was 36.5 months in 10/31 patients treated with surgery combined with chemotherapy and radiotherapy, 9.1 months in 6/31 treated with surgery plus chemotherapy, 22.4 months in 3/31 treated with radiotherapy only and 8.5 months in 6/31 treated with chemotherapy only. It is suggested that aggressive treatment can improve survival.

[Dysgerminoma of the ovary--a clinical report of 27 patients].

Twenty seven patients with dysgerminoma of the ovary were treated in our hospital from 1959 to 1982. 18 of them had no prior treatment and the other 9 had undergone operation and were admitted for recurrence. Among these 18 untreated patients, all were successfully operated. Apart from 4, the others were supplemented by radiotherapy and/or chemotherapy. 17 (94%) were free of disease from 3 to 24 years, only one died of tumor recurrence within 5 years after the initial treatment. The 5 year survival rate was 94%. However, among the 9 recurrent patients, 6 were treated by operation, supplemented by postoperative radiotherapy and/or chemotherapy but only 3 survived for 7, 14 and 15 years respectively without any evidence of disease. The 5 year survival rate was 33%. As those patients who received conservative operation initially gave a high recurrence rate, the authors propose that the patients be treated individually according to the condition of disease. The choice of treatment for the individual patient is discussed.

[Treatment of 63 advanced ovarian carcinomas].

62 patients with advanced epithelial ovarian adenocarcinoma (37 stage III, 4 stage IV and 22 recurrent lesions) were treated in our hospital from 1977 to 1981, 38 patients underwent surgery. 25 with inoperable tumor initially received preoperative chemotherapy (VCF protocol: vincristine + cyclophosphamide + fluorouracil chiefly) followed by surgery. The response rate of chemotherapy was 48%. Apart from 1 progressive tumor during chemotherapy, 24/25 were explored. There were 10/24 with residual tumor less than 2 cm in diameter, giving a resection rate of 41.7% as compared with the resection rate of 42.1% (16/38) in the operation group. It is shown that the preoperative chemotherapy improves the resection rate. 44 patients received postoperative chemotherapy and 12, radiotherapy combined with chemotherapy or radiotherapy only. The overall 2.3 and 5 year survival rates of these 63 patients were 39.7%, 31.7% and 23.8%. The 2 and 3 year survivals were 41.9% and 32.3% in 31 patients with postoperative chemotherapy using VCF protocol. The results indicate that VCF protocol is effective for advanced epithelial ovarian cancer, especially patients with residual tumor less than 2 cm in size. Chemotherapy with more than 3 courses has better effect.

A multiplex touchdown PCR for detection of Streptococcus pneumoniae, Haemophilus influenzae type b and Mycobacterium tuberculosis complex in sputum samples.

Rapid and accurate detection of Streptococcus pneumoniae (Sp), Haemophilus influenzae type b (Hib) and Mycobacterium tuberculosis complex (MTBC) in sputum by conventional methods remains problematic. Primers based on capsular polysaccharide biosynthesis gene (cpsA), the region II of the capsulation locus (cap), the insertion sequence IS6110 were designed for Sp, Hib, MTBC respectively. These primers were incorporated in a multiplex touchdown PCR assay for simultaneous detection of Sp, Hib and MTBC. The multiplex touchdown PCR assay was evaluated using standard strains and clinical sputum samples. The multiplex touchdown PCR assay showed 100% specificity in identifying Sp, Hib, MTBC from pure culture of standard strains. The sensitivities of the multiplex touchdown PCR assay were 94%, 98%, 98% for detection of Sp, Hib and MTBC respectively based on culture results while evaluated using 492 consecutive qualified clinical sputum samples; the specificities were all 100%. This highly sensitive and specific multiplex touchdown PCR assay offers a rapid and simple method for detection of Sp, Hib and MTBC in clinical sputum samples.

[Effect of moxibustion on the antibody-dependent cell-mediated cytotoxicity activity in peripheral blood of patients with Hashimoto's thyroiditis].

To investigate the immune regulating effect of moxibustion on the patients with Hashimoto's thyroiditis, 35 patients were treated by moxibustion and the changes of antibody-dependent cell-mediated cytotoxicity (ADCC) activity in the peripheral blood of the patients and their relationship with thyroid microsomal antibody (MCA) before and after treatment were observed. It was found that the activity of ADCC and the combining rate of MCA were higher than normal and they were closely related to each other. After treatment, both of them declined remarkably and the relationship between them remained the same. The result indicated that the treatment of Hashimoto's thyroiditis by moxibustion might well be related to its effect of decreasing the activity of ADCC and the titer of anti-thyroid antibodies.

Astrogliosis in culture. IV. Effects of basic fibroblast growth factor.

Previous studies have shown that the mechanical wounding of 3-week-old cultured rat astrocytes results in cell proliferation and hypertrophy resembling astrocyte responses to a brain injury in vivo. We now report the effects of basic fibroblast growth factor (bFGF) and an anti-bFGF antibody on astrocyte morphology, proliferation, and migration following in vitro wounding of confluent secondary cultures. Addition of bFGF (20 ng/ml) to wounded cultures induced morphological changes characteristic of differentiation in wounded and nonwounded areas of the culture. Combined treatment with bFGF and an anti-bFGF antibody (100 micrograms/ml) prevented this effect. Astrocyte proliferation along the edges of a scratch wound was at maximum 24 hr after wounding in cells growing in Eagle's minimum essential medium (EMEM) containing 10% serum. Low serum concentration and treatment with dibutyryl cyclic adenosine monophosphate (dbc-AMP) reduced injury-associated astrocyte proliferation. Addition of bFGF to cultures in EMEM with serum increased astrocyte proliferation at 18 and 24 hr after wounding. This effect was reduced considerably by treatment of cultures with bFGF in combination with an anti-bFGF antibody. The combined treatment and the antibody alone reduced cell division to a level lower than in control cultures. Twenty-four hr following wounding, astrocytes along the edges of the wound exhibited extension of thick, flat processes into the wound area. At 3 and 5 days after wounding, a bodily migration of astrocytes into the wounded area was observed. Addition of bFGF significantly increased astrocyte migration 1 day after wounding, with maximum effect on day 3 and no subsequent increase on day 5. A combination of bFGF and anti-bFGF antibody as well as the antibody alone reduced astrocyte migration to a level lower than in controls. Immunohistochemical localization and isoform pattern of bFGF in astrocytes did not change with dbc-AMP treatment or wounding. We conclude that mechanically wounded confluent astrocytes respond to bFGF added to the culture medium by enhancing cell division, differentiation, and migration. In addition, the results of the antibody treatment also suggest a role for endogenous bFGF in astrocyte proliferation and migration elicited by wounding in vitro. These results support the notion that in vivo, both bFGF released by injury and endogenous bFGF synthesized by astrocytes, contribute to the cellular responses that lead to astrogliosis.