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Although numerous studies have been conducted on hybrid speciation, our understanding of this process remains limited. Through an 18-year systematic investigation of all taxa of Populus on the Qinghai-Tibet Plateau, we discovered three new taxa with clear characteristics of sect. Leucoides. Further evidence was gathered from morphology, whole-genome bioinformatics, biogeography, and breeding to demonstrate synthetically that they all originated from distant hybridization between sect. Leucoides and sect. Tacamahaca. P. gonggaensis originated from the hybridization of P. lasiocarpa with P. cathayana, P. butuoensis from the hybridization of P. wilsonii with P. szechuanica, and P. dafengensis from the hybridization of P. lasiocarpa with P. szechuanica. Due to heterosis, the three hybrid taxa possess greater ecological adaptability than their ancestral species. We propose a hybrid speciation process model that incorporates orthogonal, reverse, and backcrossing events. This model can adequately explain some crucial evolutionary concerns, such as the nuclear-cytoplasmic conflict on phylogeny and the extinction of ancestral species within the distribution range of hybrid species.
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Populus , Filogenia , Populus/genética , Evolução Biológica , Hibridização Genética , Hibridização de Ácido NucleicoRESUMO
While the diversity of species formation is broadly acknowledged, significant debate exists regarding the universal nature of hybrid species formation. Through an 18-year comprehensive study of all Populus species on the Qinghai-Tibet Plateau, 23 previously recorded species and 8 new species were identified. Based on morphological characteristics, these can be classified into three groups: species in section Leucoides, species with large leaves, and species with small leaves in section Tacamahaca. By conducting whole-genome re-sequencing of 150 genotypes from these 31 species, 2.28 million single nucleotide polymorphisms (SNPs) were identified. Phylogenetic analysis utilizing these SNPs not only revealed a highly intricate evolutionary network within the large-leaf species of section Tacamahaca but also confirmed that a new species, P. curviserrata, naturally hybridized with P. cathayana, P. szechuanica, and P. ciliata, resulting in 11 hybrid species. These findings indicate the widespread occurrence of hybrid species formation within this genus, with hybridization serving as a key evolutionary mechanism for Populus on the plateau. A novel hypothesis, "Hybrid Species Exterminating Their Ancestral Species (HSEAS)," is introduced to explain the mechanisms of hybrid species formation at three different scales: the entire plateau, the southeastern mountain region, and individual river valleys.
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Especiação Genética , Hibridização Genética , Filogenia , Polimorfismo de Nucleotídeo Único , Populus , Populus/genética , Populus/classificação , TibetRESUMO
Excess nitrogen (N) discharged into streams and rivers degrades freshwater quality and threatens ecosystems worldwide. Land use patterns may influence riverine N export, yet the effect of location on N export and removal is not fully understood. We proposed a hybrid model to analyze N export and removal within the watersheds. The proposed model is satisfied for the riverine N modelling. The KGE and R2 are 0.75 and 0.72 in the calibration period which are 0.76 and 0.61 in the validation period. Human-impacted land use may modify the N yield in the watershed, and the net N export from built-up to the in-stream system was highest in the urbanized sub-watersheds (0.81), followed by the agricultural sub-watersheds (0.88), and forested sub-watersheds (0.96). Agricultural activities make a large contribution to the N exports in the watersheds, and the mean N input from the agricultural land use to in-stream were 2069-4353 kg km-2 yr-1. Besides, the excess inputs of N by overapplication of fertilizer and manure during the agricultural activities may increase legacy N in soil and groundwater. Biological processes for the riverine N removal may be controlled by the available substrate in the freshwater system, and temperature sensitivity of denitrification is highest in the flood seasons, especially for the human-impacted sub-watersheds. The riverine biological processes may be limited by other competitions. Our model results provide evidence that quantity and location of specific land use may control biogeochemistry within watersheds. We demonstrate the need to understand nutrient export and removal within watersheds by improving the representation of spatial patterns in existing watershed models, and we consider this study to be a new effort for the spatially explicit modeling to support land-use based N management in watersheds.
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Agricultura , Nitrogênio , Rios , Nitrogênio/análise , Rios/química , Modelos Teóricos , Ecossistema , Nutrientes/análise , Monitoramento AmbientalRESUMO
A sandwiched piezoelectric accelerometer is developed and optimized for acquiring low-frequency, wide-band seismic data. The proposed accelerometer addresses the challenges of capturing seismic signals in the low-frequency range while maintaining a broad frequency response through the design of multi-stage charge amplifiers and a sandwiched structure. The device's design, fabrication process, and performance evaluation are discussed in detail. Experimental results demonstrate its performance in amplitude and phase response characteristics.
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Water security is a critical concern due to intensifying anthropogenic activities and climate change. Delineating a water-related ecological security pattern can help to optimize spatial configuration, which in turn can inform sustainable water management. However, the methodology remains unclear. In this study, we developed a framework linking ecosystem service flow to water-related ecological security pattern; hence, we identified the sources, sinks, key corridors, and vulnerable nodes in Fujian Province, China. Our results revealed that the sources were located inland at high altitudes with a decreasing area trend in the south and an increasing area trend in the north, whereas the sinks were spread in coastal areas and exhibited a decreasing trend with relatively stable spatial distribution. The water-related ecological security has degraded as represented by a decreasing ecological supply-demand ratio over the last 30 years. Key corridors were identified in 17.12% of the rivers, and 22.5% of the vulnerable nodes were recognized as early warning nodes. Climate variability affected source distribution, while anthropogenic activities drove sink dynamics. These findings have important implications including landscape pattern planning and sustainable water management in the context of accelerated land use/cover and climate changes.
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Conservação dos Recursos Naturais , Ecossistema , China , Rios , Efeitos AntropogênicosRESUMO
mascRNA is a small cytoplasmic RNA derived from the lncRNA MALAT1. After being processed by the tRNA processing enzymes RNase P and RNase Z, mascRNA undergoes CCA addition like tRNAs and folds into a tRNA-like cloverleaf structure. While MALAT1 functions in multiple cellular processes, the role of mascRNA was largely unknown. Here, we show that mascRNA binds directly to the multi-tRNA synthetase complex (MSC) component glutaminyl-tRNA synthetase (QARS). mascRNA promotes global protein translation and cell proliferation by positively regulating QARS protein levels. Our results uncover a role of mascRNA that is independent of MALAT1, but could be part of the molecular mechanism of MALAT1's function in cancer, and provide a paradigm for understanding tRNA-like structures in mammalian cells.
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RNA Longo não Codificante , Pequeno RNA não Traduzido , Animais , Biossíntese de Proteínas , Processamento Pós-Transcricional do RNA , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , RNA de Transferência/genética , RNA de Transferência/metabolismoRESUMO
As a highly contagious disease, COVID-19 caused a worldwide pandemic and it is still ongoing. However, the infection in China has been successfully controlled although its initial transmission was also nationwide and has caused a serious public health crisis. The analysis on the early-stage COVID-19 transmission in China is worth investigating for its guiding significance on prevention to other countries and regions. In this study, we conducted the experiments from the perspectives of COVID-19 occurrence and intensity. We eliminated unimportant factors from 113 variables and applied four machine learning-based classification and regression models to predict COVID-19 occurrence and intensity, respectively. The influence of each important factor was analysed when applicable. Our optimal model on COVID-19 occurrence prediction presented an accuracy of 91.91% and the best R2 of intensity prediction reached 0.778. Linear regression-based model was identified as unable to fit and predict the intensity, and thus only the variable influence on COVID-19 occurrence can be explained. We found that (1) CO VID-19 was more likely to occur in prosperous cities closer to the epicentre and located on higher altitudes, (2) and the occurrence was higher under extreme weather and high minimum relative humidity. (3) Most air pollutants increased the risk of COVID-19 occurrence except NO2 and O3, and there existed a lag effect of 6-7 days. (4) NPIs (non-pharmaceutical interventions) did not show apparent effect until two weeks after.
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Poluentes Atmosféricos , Poluição do Ar , COVID-19 , Poluentes Atmosféricos/análise , Poluição do Ar/análise , COVID-19/epidemiologia , China/epidemiologia , Cidades , Humanos , Aprendizado de Máquina , Material Particulado/análise , SARS-CoV-2 , Fatores SociaisRESUMO
Metal(loid)s in cultivated land become an important issue with respect to human health and food security. However, it remains challenging to identify metal(loid) pollution characteristics due to varying environmental settings at the local scale. In this study, the geographic information system and categorical regression model were applied to analyze the spatial distribution and influencing factors of metal(loid)s in cultivated land using 90 sampling sites in Xianjia Town, Southeast China. The pollution levels and ecological risks of five metal(loid)s-Cd, Pb, Cr, Hg, and As-were further investigated using the single pollution index (PI), Nemerow comprehensive pollution index (PN), and potential ecological risk index (RI). The results indicate that the cultivated soils were affected by Cd and Pb pollution, with 3.06 and 6.30 times higher average concentrations than the soil environment background values (SEBV) of Fujian Province, respectively. Based on the CATREG model, crop type had a great impact on Pb and Hg contents. Cr contents were higher in rice fields, while Hg and As concentrations were higher in turmeric fields. Cr and Hg contents under five crop types did not exceed the SEBV of Fujian Province. The average Pb contents in rice fields were 1.25 and the Cd contents in vegetable fields 1.09 times higher than the average value in sampled soils. According to the RI, 63.66% of the sampling points were at medium to high risk. These findings enhance our understanding of the metal(loid)s pollution characteristics and their ecological risks in cultivated land at the local scale.
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Mercúrio , Metais Pesados , Oryza , Poluentes do Solo , Cádmio , China , Monitoramento Ambiental , Humanos , Chumbo , Metais Pesados/análise , Solo , Poluentes do Solo/análiseRESUMO
BACKGROUND: Trees such as Populus are planted extensively for reforestation and afforestation. However, their successful establishment greatly depends upon ambient environmental conditions and their relative resistance to abiotic and biotic stresses. Polyphenol oxidase (PPO) is a ubiquitous metalloproteinase in plants, which plays crucial roles in mediating plant resistance against biotic and abiotic stresses. Although the whole genome sequence of Populus trichocarpa has long been published, little is known about the PPO genes in Populus, especially those related to drought stress, mechanical damage, and insect feeding. Additionally, there is a paucity of information regarding hormonal responses at the whole genome level. RESULTS: A genome-wide analysis of the poplar PPO family was performed in the present study, and 18 PtrPPO genes were identified. Bioinformatics and qRT-PCR were then used to analyze the gene structure, phylogeny, chromosomal localization, gene replication, cis-elements, and expression patterns of PtrPPOs. Sequence analysis revealed that two-thirds of the PtrPPO genes lacked intronic sequences. Phylogenetic analysis showed that all PPO genes were categorized into 11 groups, and woody plants harbored many PPO genes. Eighteen PtrPPO genes were disproportionally localized on 19 chromosomes, and 3 pairs of segmented replication genes and 4 tandem repeat genomes were detected in poplars. Cis-acting element analysis identified numerous growth and developmental elements, secondary metabolism processes, and stress-related elements in the promoters of different PPO members. Furthermore, PtrPPO genes were expressed preferentially in the tissues and fruits of young plants. In addition, the expression of some PtrPPOs could be significantly induced by polyethylene glycol, abscisic acid, and methyl jasmonate, thereby revealing their potential role in regulating the stress response. Currently, we identified potential upstream TFs of PtrPPOs using bioinformatics. CONCLUSIONS: Comprehensive analysis is helpful for selecting candidate PPO genes for follow-up studies on biological function, and progress in understanding the molecular genetic basis of stress resistance in forest trees might lead to the development of genetic resources.
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Catecol Oxidase , Proteínas de Plantas/genética , Populus , Catecol Oxidase/genética , Secas , Regulação da Expressão Gênica de Plantas , Filogenia , Populus/enzimologia , Populus/genética , Estresse FisiológicoRESUMO
Poplar are planted extensively in reforestation and afforestation. However, their successful establishment largely depends on the environmental conditions of the newly established plantation and their resistance to abiotic as well as biotic stresses. NF-X1, a widespread transcription factor in plants, plays an irreplaceable role in plant growth, development, and stress tolerance. Although the whole genome sequence of Populus trichocarpa has been published for a long time, little is known about the NF-X1 genes in poplar, especially those related to drought stress, mechanical damage, insect feeding, and hormone response at the whole genome level. In this study, whole genome analysis of the poplar NF-X1 family was performed, and 4 PtrNF-X1 genes were identified. Then, bioinformatics analysis and qRT-PCR were applied to analyze the gene structure, phylogeny, chromosomal localization, gene replication, Cis-elements, and expression patterns of PtrNF-X1genes. Sequence analysis revealed that one-quarter of the PtrNF-X1 genes did not contain introns. Phylogenetic analysis revealed that all NF-X1 genes were split into three subfamilies. The number of two pairs of segmented replication genes were detected in poplars. Cis-acting element analysis identified a large number of elements of growth and development and stress-related elements on the promoters of different NF-X1 members. In addition, some PtrNF-X1 could be significantly induced by polyethylene glycol (PEG) and abscisic acid (ABA), thus revealing their potential role in regulating stress response. Comprehensive analysis is helpful in selecting candidate NF-X1 genes for the follow-up study of the biological function, and molecular genetic progress of stress resistance in forest trees provides genetic resources.
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Genes de Plantas , Proteínas de Plantas/genética , Populus/genética , Fatores de Transcrição/genética , Arabidopsis/genética , Cromossomos de Plantas/genética , Evolução Molecular , Duplicação Gênica , Perfilação da Expressão Gênica , Regulação da Expressão Gênica no Desenvolvimento , Regulação da Expressão Gênica de Plantas , Genoma de Planta , Família Multigênica , Oryza/genética , Filogenia , Populus/crescimento & desenvolvimento , Especificidade da Espécie , Estresse Fisiológico/genética , SinteniaRESUMO
Land use change simulation is an important issue for its role in predicting future trends and providing implications for sustainable land management. Hybrid models have become a recognized strategy to inform decision-makers, but further attempts are needed to warrant the reliability of their projected results. In view of this, three hybrid models, including the cellular automata-Markov chain-artificial neural network, cellular automata-Markov chain-logistic regression, and Markov chain-artificial neural network, were applied to simulate land use change on the largest island in Iran, Qeshm Island. The Figure of Merit (FOM) was used to measure the modeling accuracy of the simulations, with the FOMs for the three models 6.7, 5.1, and 4.5, respectively. Consequently, the cellular automata-Markov chain-artificial neural network most precisely simulates land use change on Qeshm Island and is, thus, used to simulate land use change until 2026. The simulation shows that the incremental trend of the built-up class will continue in the coming years. Meanwhile, the areas of valuable ecosystems, such as mangroves, tend to decrease. Despite the protection plans for mangroves, these areas require more attention and conservation planning. This study demonstrates a referential example to select the proper land use models for informing planning and management in similar coastal zones.
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Conservação dos Recursos Naturais , Ecossistema , Monitoramento Ambiental , Irã (Geográfico) , Ilhas , Reprodutibilidade dos TestesRESUMO
Mitochondrial proteins are encoded in both mitochondrial and nuclear genomes. The expression levels of these two pools of mitochondrial genes are co-regulated and synchronized. Import and assembly of the nucleus-encoded oxidative phosphorylation (OXPHOS) subunits affect protein synthesis in the mitochondrial matrix by engaging the mitochondrial ribosomes. How the ribosomes at the outside of mitochondria are regulated by mitochondria, however, remains mostly unexplored. Here, using an array of biochemical assays and genetic knockdown and overexpression in HEK293 or mouse cells, we show that cytosolic rRNAs that are associated with the mitochondrial outer membrane have very different decay patterns from those of both endoplasmic reticulum-associated and -nonassociated cytosolic rRNAs. Mitochondrial intermembrane space RNase T2 (RNASET2), which has been previously shown to degrade mitochondrial RNAs, is also responsible for selective degradation of the cytosolic rRNAs on the outer membrane. We noted that the degradation activity also has a positive effect on nuclear transcription of rRNAs, suggesting a compensatory feedback mechanism, and affects protein translations in and out of mitochondria. These findings establish a mechanism for the co-regulation of gene expression programs inside and outside of mitochondria in mammalian cells.
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Citosol/metabolismo , Mitocôndrias/metabolismo , Ribonucleases/metabolismo , Ribossomos/metabolismo , Proteínas Supressoras de Tumor/metabolismo , Células HEK293 , Humanos , Transporte Proteico , RNA Ribossômico/metabolismoRESUMO
Phytoremediation soil polluted by cadmium has drawn worldwide attention. However, how to improve the efficiency of plant remediation of cadmium contaminated soil remains unknown. Previous studies showed that nitrogen (N) significantly enhances cadmium uptake and accumulation in poplar plants. In order to explore the important role of nitrogen in plants' responses to cadmium stress, this study investigates the poplar proteome and phosphoproteome difference between Cd stress and Cd + N treatment. In total, 6573 proteins were identified, and 5838 of them were quantified. With a fold-change threshold of > 1.3, and a p-value < 0.05, 375 and 108 proteins were up- and down-regulated by Cd stress when compared to the control, respectively. Compared to the Cd stress group, 42 and 89 proteins were up- and down-regulated by Cd + N treatment, respectively. Moreover, 522 and 127 proteins were up- and down-regulated by Cd + N treatment compared to the CK group. In addition, 1471 phosphosites in 721 proteins were identified. Based on a fold-change threshold of > 1.2, and a p-value < 0.05, the Cd stress up-regulated eight proteins containing eight phosphosites, and down-regulated 58 proteins containing 69 phosphosites, whereas N + Cd treatment up-regulated 86 proteins containing 95 phosphosites, and down-regulated 17 proteins containing 17 phosphosites, when compared to Cd stress alone. N + Cd treatment up-regulated 60 proteins containing 74 phosphosites and down-regulated 37 proteins containing 42 phosphosites, when compared to the control. Several putative responses to stress proteins, as well as transcriptional and translational regulation factors, were up-regulated by the addition of exogenous nitrogen following Cd stress. Especially, heat shock protein 70 (HSP70), 14-3-3 protein, peroxidase (POD), zinc finger protein (ZFP), ABC transporter protein, eukaryotic translation initiation factor (elF) and splicing factor 3 B subunit 1-like (SF3BI) were up-regulated by Cd + N treatment at both the proteome and the phosphoproteome levels. Combing the proteomic data and phosphoproteomics data, the mechanism by which exogenous nitrogen can alleviate cadmium toxicity in poplar plants was explained at the molecular level. The results of this study will establish the solid molecular foundation of the phytoremediation method to improve cadmium-contaminated soil.
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Cádmio/uso terapêutico , Nitrogênio/farmacologia , Fosfoproteínas/metabolismo , Proteínas de Plantas/metabolismo , Populus/metabolismo , Proteoma/metabolismo , Fosforilação/efeitos dos fármacosRESUMO
Brown adipose tissue (BAT) is specialized to dissipate energy as heat, therefore reducing fat deposition and counteracting obesity. Brown adipocytes arise from myoblastic progenitors during embryonic development by the action of transcription regulator PRDM16 binding to PPARγ, which promotes BAT-like phenotype in white adipose tissue. To investigate the capability of converting white adipose tissue to BAT or browning by PPARγ in vivo, we generated transgenic mice with over-expressed PPARγ2. The transgenic mice showed strong brown fat features in subcutaneous fat in morphology and histology. To provide molecular evidences on browning characteristics of the adipose tissue, we employed quantitative real-time PCR to determine BAT-specific gene expressions. The transgenic mice had remarkably elevated mRNA level of UCP1, Elovl3, PGC1α and Cebpα in subcutaneous fat. Compared with wild-type mice, UCP1 protein levels were increased significantly in transgenic mice. ATP concentration was slightly decreased in the subcutaneous fat of transgenic mice. Western blotting analysis also confirmed that phosphorylated AMPK and ACC proteins were significantly (P<0.01) increased in the transgenic mice. Therefore, this study demonstrated that over-expression of PPARγ2 in skeletal muscle can promote conversion of subcutaneous fat to brown fat formation, which can have beneficial effects on increasing energy metabolisms and combating obesity.
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Tecido Adiposo Marrom/crescimento & desenvolvimento , PPAR gama/genética , Regulação para Cima , Proteínas Quinases Ativadas por AMP/metabolismo , Acetil-CoA Carboxilase/metabolismo , Trifosfato de Adenosina/metabolismo , Tecido Adiposo Marrom/metabolismo , Tecido Adiposo Branco/metabolismo , Animais , Feminino , Regulação da Expressão Gênica no Desenvolvimento , Canais Iônicos/genética , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Mitocôndrias/metabolismo , Proteínas Mitocondriais/genética , PPAR gama/metabolismo , RNA Mensageiro/genética , Gordura Subcutânea/crescimento & desenvolvimento , Gordura Subcutânea/metabolismo , Transgenes , Proteína Desacopladora 1RESUMO
The aim of this study is to improve the understanding of land changes in the Jiulong River watershed, a coastal watershed of Southeast China. We developed a stratified classification methodology for land mapping, which combines linear stretching, an Iterative Self-Organizing Data Analysis (ISODATA) clustering algorithm, and spatial reclassification. The stratified classification for 2002 generated less overall error than an unstratified classification. The stratified classifications were then used to examine temporal differences at 1986, 1996, 2002, 2007 and 2010. Intensity Analysis was applied to analyze land changes at three levels: time interval, category, and transition. Results showed that land use transformation has been accelerating. Woodland's gains and losses were dormant while the gains and losses of Agriculture, Orchard, Built-up and Bare land were active during all time intervals. Water's losses were active and stationary. The transitions from Agriculture, Orchard, and Water to Built-up were systematically targeting and stationary, while the transition from Woodland to Built-up was systematically avoiding and stationary.
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A template-based solvothermal method was successfully developed for the controlled synthesis of two-dimensional (2D) monoclinic WO3 nanoplate/nanosheet arrays and three-dimensional (3D) hexagonal WO3 nanosphere/nanocage structures with single crystal petals. The structure-directing agents played an important role in controlling the morphology and phase of WO3 samples. The results showed that the WO3 nanospheres exhibited the highest visible light absorption capacity and a photocurrent density of 0.37 mA cm-2 at 1.23 V vs. RHE under simulated sunlight. Moreover, the photocatalytic dye results displayed 83.2% methylene blue degradation and 87.9% rhodamine B degradation within 120 min under visible light irradiation. The high performance of the WO3 nanospheres, resulted from the hierarchical structure, increased surface area and enhanced light absorption, which improved the photogenerated charge carrier transfer and separation capability.
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Fluorescence sensing of latent fingerprints (LFPs) has gained extensive attention due to its high sensitivity, non-destructive testing, low biotoxicity, ease of operation, and the potential for in situ visualization. However, the realization of in situ visualization of LFPs especially with green emission and rapid speed is still a challenge. Herein, we synthesized an amphibious green-emission AIE-gen TPE-NI-AOH (PLQY = 62%) for instant in situ LFP detecting, which integrates the excellent fluorescence properties of naphthalimide (NI) with a hydrophilic head and the AIE character as well as the donating property of tetraphenylethene (TPE). TPE-NI-AOH in ethanol/water binary solvent was used as an environmentally friendly LFP developer and achieved in situ green-fluorescence visualization of LFPs. The fluorescence signal achieves its 60% saturated intensity in 0.37 s and nearly 100% in 2.50 s, which is an instant process for the naked eye. Moreover, level 3 details and super-resolution images of LFPs could be observed clearly. Besides, the TPE-NI-AOH developer could be stored for at least 6 months, suitable for long-term storage. This instant in situ highlighting method does not require post-processing operations, providing a more convenient, rapid, and efficient detection method of LFPs. This work would inspire the further advancement of fluorescent sensors for fingerprint imaging.
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INTRODUCTION: Growing attention has been drawn to urologic tumors due to their rising incidence and suboptimal clinical treatment outcomes. Cancer therapy resistance poses a significant challenge in clinical oncology, limiting the efficacy of conventional treatments and contributing to disease progression. Recent research has unveiled a complex interplay between the host microbiota and cancer cells, highlighting the role of the microbiota in modulating therapeutic responses. AREAS COVERED: We used the PubMed and Web of Science search engines to identify key publications in the fields of tumor progression and urologic tumor treatment, specifically focusing on the role of the microbiota. In this review, we summarize the current literature on how microbiota influence the tumor microenvironment and anti-tumor immunity, as well as their impact on treatments for urinary system malignancies, highlighting promising future applications. EXPERT OPINION: We explore how the composition and function of the gut microbiota influence the tumor microenvironment and immune response, ultimately impacting treatment outcomes. Additionally, we discuss emerging strategies targeting the microbiota to enhance therapeutic efficacy and overcome resistance. The application of antibiotics, fecal microbiota transplantation, and oncolytic bacteria has improved tumor treatment outcomes, which provides a novel insight into developing therapeutic strategies for urologic cancer.
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Intravitreal (IVT) injection of anti-vascular endothelial growth factor (anti-VEGF) has greatly improved the treatment of many retinal disorders, including wet age-related macular degeneration (wAMD), which is the third leading cause of blindness. However, frequent injections can be difficult for patients and may lead to various risks such as elevated intraocular pressure, infection, and retinal detachment. To address this issue, researchers have found that IVT injection of anti-VEGF proteins at their maximally viable concentration and dose can be an effective strategy. However, the intrinsic protein structure can limit the maximum concentration due to stability and solution viscosity. To overcome this challenge, we developed a novel anti-VEGF protein called nanoFc by fusing anti-VEGF nanobodies with a crystallizable fragment (Fc). NanoFc has demonstrated high binding affinity to VEGF165 through multivalency and potent bioactivity in various bioassays. Furthermore, nanoFc maintains satisfactory chemical and physical stability at 4°C over 1 month and is easily injectable at concentrations up to 200 mg/mL due to its unique architecture that yields a smaller shape factor. The design of nanoFc offers a bioengineering strategy to ensure both strong anti-VEGF binding affinity and high protein concentration, with the goal of reducing the frequency of IV injections.
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Forest trees face many abiotic stressors during their lifetime, including drought, heavy metals, high salinity, and chills, affecting their quality and yield. The RING-type ubiquitin ligase E3 is an invaluable component of the ubiquitin-proteasome system (UPS) and participates in plant growth and environmental interactions. Interestingly, only a few studies have explored the RING ZINC FINGER PROTEIN (RZFP) gene family. This study identified eight PtrRZFPs genes in the Populus genome, and their molecular features were analyzed. Gene structure analysis revealed that all PtrRZFPs genes contained >10 introns. Evolutionarily, the RZFPs were separated into four categories, and segmental replication events facilitated their amplification. Notably, many stress-related elements have been identified in the promoters of PtrRZFPs using Cis-acting element analysis. Moreover, some PtrRZFPs were significantly induced by drought and sorbitol, revealing their potential roles in regulating stress responses. Particularly, overexpression of the PtrRZFP1 gene in poplars conferred excellent drought tolerance; however, PtrRZFP1 knockdown plants were drought-sensitive. We identified the potential upstream transcription factors of PtrRZFPs and revealed the possible biological functions of RZFP1/4/7 in resisting osmotic and salt stress, laying the foundation for subsequent biological function studies and providing genetic resources for genetic engineering breeding for drought resistance in forest trees. This study offers crucial information for the further exploration of the functions of RZFPs in poplars.