RESUMO
The changes of microRNA expression in rat hippocampus after traumatic brain injury (TBI) were explored. Adult SD rats received a single controlled cortical impact injury, and the ipsilateral hippocampus was harvested for the subsequent microarray assay at three time points after TBI: 1st day, 3rd day and 5th day, respectively. We characterized the microRNA expression profile in rat hippocampus using the microRNA microarray analysis, and further verified microarray results of miR-142-3p and miR-221 using quantitative real-time PCR. Totally 205 microRNAs were identified and up-/down-regulated more than 1.5 times. There were significant changes in 17 microRNAs at all three time points post-TBI. The quantitative real-time PCR results of miR-142-3p and miR-221 indicated good consistency with the results of the microarray method. MicroRNAs altered at different time points post-TBI. MiR-142-3p and miR-221 may be used as potentially biological markers for TBI assessment in forensic practice.
Assuntos
Lesões Encefálicas/metabolismo , Regulação da Expressão Gênica , Hipocampo/metabolismo , MicroRNAs/biossíntese , Animais , Biomarcadores/metabolismo , Lesões Encefálicas/patologia , Feminino , Genética Forense , Perfilação da Expressão Gênica , Hipocampo/patologia , Masculino , Ratos , Ratos Sprague-DawleyRESUMO
OBJECTIVE: To discuss characteristics of height growth such as Peak Height Velocity (PHV) and Age at Peak Height Velocity (PHA) during adolescence, and to compare the results with other research findings. METHODS: Primary and middle school students' annual physical examination data of Zhongshan in 2005 - 2010 was used. The height velocity by age, PHV, PHA, height velocity by PHA were calculated. RESULTS: The average peak height velocity boys was (10.03 ± 1.67) cm/yr. and that of the girls was (8.39 ± 1.05) cm/yr. Both findings were close to the results from previous similar findings. The average age at which peak height velocity reached 12.28 ± 1.30 years for boys and 10.78 ± 1.04 years for girls, both lower than the previous findings. The correlation coefficients, between height level and PHA were -0.357 (P < 0.001) for boys and -0.338 (P < 0.001) for girls. CONCLUSION: The height levels were positively related to the height velocity before PHA. The Zhongshan students' PHA was lower than the Beijing, Shanghai and Shenyang students, also lower than American and Britain students', but their PHVs were similar.