An introduced and a native vertebrate hybridize to form a genetic bridge to a second native species.

The genetic impacts of hybridization between native and introduced species are of considerable conservation concern, while the possibility of reticulate evolution affects our basic understanding of how species arise and shapes how we use genetic data to understand evolutionary diversification. By using mitochondrial NADH dehydrogenase subunit 2 (ND2) sequences and 467 amplified fragment-length polymorphism nuclear DNA markers, we show that the introduced white sucker (Catostomus commersoni) has hybridized with two species native to the Colorado River Basin--the flannelmouth sucker (Catostomus latipinnis) and the bluehead sucker (Catostomus discobolus). Hybrids between the flannelmouth sucker and white sucker have facilitated introgression between the two native species, previously isolated by reproductive barriers, such that individuals exist with contributions from all three genomes. Most hybrids had the mitochondrial haplotype of the introduced white sucker, emphasizing its pivotal role in this three-way hybridization. Our findings highlight how introduced species can threaten the genetic integrity of not only one species but also multiple previously reproductively isolated species. Furthermore, this complex three-way reticulate (as opposed to strictly bifurcating) evolution suggests that seeking examples in other vertebrate systems might be productive. Although the present study involved an introduced species, similar patterns of hybridization could result from natural processes, including stream capture or geological formations (e.g., the Bering land bridge).

Infection by a black spot-causing species of Uvulifer and associated opercular alterations in fishes from a high-desert stream in Wyoming.

Black spot is a common disease syndrome of freshwater fishes. This study provides information on the rank of density of the black spot agent and opercular bone alterations associated with at least one digenean, Uvulifer sp., infecting native and non-native catostomids and cyprinids of the Upper Colorado River Basin. We evaluated the density rank of pigmented metacercariae and associated alterations in the operculum of the bluehead sucker Catostomus discobolus, flannelmouth sucker C. latipinnis, white sucker C. commersoni, catostomid hybrids, roundtail chub Gila robusta, and creek chub Semotilus atromaculatus, sampled from Muddy Creek, Wyoming, USA in 2003 or 2004. All fish species contained individuals that exhibited gross signs of the black spot agent. Bluehead and flannelmouth suckers had 100% prevalence of infection. Although the other suckers and chubs contained encysted metacercariae in at least one individual, the presence of pigmented metacercariae was not apparent (i.e. based on gross observations) in many individuals. Catostomids had higher densities of metacercariae than cyprinids, as shown by frequency distributions of density ranks. Opercular holes (i.e. holes that completely penetrated the opercle and were in direct association with the pigment associated metacercariae) and pockets (depressions on the external surface of the opercle associated with metacercariae) were abundant among catostomids but rare among cyprinids.

Factors Influencing the Size Structure of Brook Trout and Brown Trout in Southeastern Wyoming Mountain Streams.

We used discriminant models to identify relations among size structures of stocks of brook trout Salvelinus fontinalis and brown trout Salmo trutta and features of their habitats in small mountain streams (2,377-2,975 m above mean sea level) in the Medicine Bow National Forest, Wyoming. Size structure was predicted from position of the study reach in the watershed, from channel gradient, and from composition of the salmonid community. Brook trout were predominantly small in high-elevation, moderate-gradient, forested reaches with allopatric populations; they were larger in mid-elevation, low-gradient, meadow reaches that contained some brown trout. Brown trout were mostly small in mid-elevation, moderate-gradient, forested reaches; however, more large brown trout occurred lower in the watersheds, in meadow or rangeland stream reaches that had low gradients and that supported allopatric populations.

Trout population responses to restoration of stream flows.

There are numerous demands for the limited water supplies in the Rocky Mountain (USA) region, and controversies surrounding instream flows abound. A specific problem involves water diversions (i.e., small dams that shunt water out of stream channels) during the summer irrigation season. We developed an approach to assess the effects of restoration of natural or less-than-natural summer flows on trout that accounts for variation in habitat over long segments of low-gradient, alluvial-valley streams. The approach has utility for managers because it can be conducted with hydologic data, aerial photographs, topographic maps, and a spreadsheet without extensive fieldwork. We applied the approach by assessing the effects of different summer flows on abundance of brown trout ( Salmo trutta) in several streams annually dewatered in the Salt River Valley of western Wyoming. The assessment approach can be calibrated for other trout species and areas of the Rocky Mountain region.