Comprehensive evaluation of water quality status for entire stretch of Yamuna River, India.

This study represented the first comprehensive assessment of the physicochemical water quality status of the entire Yamuna River stretch in India. The upper zone had "excellent-to-good" water quality index (WQI) with mean 5-day biochemical oxygen demand (BOD5) values of 2.1 and 2.4 mg/L during monsoon and non-monsoon, respectively. The middle region was described by "poor-to-marginal" WQI with average BOD5 values of 13.1 mg/L (monsoon) and 32.3 mg/L (non-monsoon). The low WQI observations at the midstream region were due to the negative impact of two major drains, namely Najafgarh and Shahdara, that carry partially treated effluents from industrial units. Further, BOD5 decreased to 1.9 mg/L (monsoon) and 1.8 mg/L (non-monsoon) in the lower zone, and the WQI values improved to "good" and "excellent". The dilution and depuration effects of the Chambal, Sindh, Betwa, and Ken Rivers recovered the environmental conditions in downstream stations. The oxygen sag curve complied with the water quality status along the river stretch. Based on the principal component analysis, the Yamuna River was strongly influenced by dissolved mineral salts originating from atmospheric deposition, weathering of soils and rocks, and application of deicing chemicals and landfills. Moreover, organic and nutrient substances and biological activities resulting from the discharge of sewage, and the utilization of fertilizers in agriculture, were the second contributors to pollution. The statistical techniques employed in this work could be beneficial for decision-makers (government and stakeholders) to identify the pollution sources/factors and to determine the viability of water bodies for domestic applications.

Fluoride contamination in drinking water in rural habitations of Central Rajasthan, India.

Fluoride concentration in groundwater sources used as major drinking water source in rural area of block Nawa (Nagaur District), Rajasthan was examined and the toxic effects by intake of excess fluoride on rural habitants were studied. In block 13, habitations (30%) were found to have fluoride concentration more than 1.5 mg/l (viz. maximum desirable limit of Indian drinking water standards IS 10500, 1999). In five habitations (11%), fluoride concentration in groundwater is at toxic level (viz. above 3.0 mg/l). The maximum fluoride concentration in the block is 5.91 mg/l from Sirsi village. As per the desirable and maximum permissible limit for fluoride in drinking water, determined by World Health Organization or by Bureau of Indian Standards, the groundwater of about 13 habitations of the studied sites is unfit for drinking purposes. Due to the higher fluoride level in drinking water, several cases of dental and skeletal fluorosis have appeared at alarming rate in this region. There is an instant need to take ameliorative steps in this region to prevent the population from fluorosis. Groundwater sources of block Nawa can be used for drinking after an effective treatment in absence of other safe source. The evaluation of various defluoridation methods on the basis of social and economical structure of India reveals that the clay pot chip, activated alumina adsorption, and Nalgonda techniques are the most promising.

Heavy metal contamination in the complete stretch of Yamuna river: A fuzzy logic approach for comprehensive health risk assessment.

River Yamuna is one of the most sacred major tributaries of river Ganga. This study aimed to assess the level of heavy metals in monsoon and non-monsoon season in river Yamuna in Uttar Pradesh, India and to assess the possible source of contamination and its associated health risk. Except for iron (Fe), the mean levels of all metals were within drinking water safe limits in both seasons. Except for chromium (Cr), lower values were observed for other metals in the monsoon season could be attributed dilution effect. Multivariate analysis indicated that both geogenic and anthropogenic sources contribute to heavy metals in river Yamuna in monsoon and non-monsoon seasons. The health risk in terms of hazard index (HI) and fuzzy-logic hazard index (FHI) demonstrated that both HI and FHI values among children exceeded the safe limit in most of the sites in non-monsoon seasons and in few in monsoon season. For adults, HI and FHI values were within safe limit.

Docosahexaenoic Acid (DHA, C22:6, ω-3) Composition of Milk and Mammary Gland Tissues of Lactating Mother Rats Is Severely Affected by Lead (Pb) Exposure.

Docosahexaenoic acid (DHA, C22:6, ω-3), an ω-3 polyunsaturated fatty acid (PUFA), is critical for brain growth, development, and cognitive ability. It is consumed by offspring via milk during lactation. However, the toxic heavy metal lead (Pb) readily passes into the mammary glands of mother animals and then to offspring through milk. Here, we investigated whether DHA composition of milk and mammary gland tissues is affected by Pb exposure. Mother rats were exposed to Pb via drinking water (0.1%). The fatty acid profile and levels of reduced glutathione (GSH), lipid peroxide (LPO), and pro-inflammatory TNF-α in milk and mammary tissues were measured. Levels of DHA and antioxidant GSH decreased (P < 0.05), while LPO and TNF-α levels increased (P < 0.05) both in milk and mammary tissues. Our results suggest that toxic Pb exposure can upset the level of milk DHA, which may affect brain growth and development, and hence cognitive ability in adulthood and later life.

Cholesterol Lowering and Antioxidative Effect of Pregerminated Brown Rice in Hypercholesterolemic Rats.

Pregerminated brown rice (GBR) is assumed to be more beneficial than polished white rice (WR), with regard to nutrition and cardiovascular health. To support this with scientific evidence, cholesterol-lowering and antioxidative effects of GBR were studied in the present investigation. The most popular rice variety in Bangladesh BIRI-29 was used to prepare GBR and WR. Initially, we analyzed the proximate composition, antioxidative phytochemicals, in vitro 2, 2-diphenyl-1-picrylhydrazyl (DPPH)-free radical scavenging ability and anti-hemolytic effects of GBR. To examine the dietary impact and possible benefits of the GBR, experimentally-induced hypercholesterolemic (HC, 1% cholesterol) rats were fed with GBR against WR for 12 wk. At the end, plasma total cholesterol (TC), low- and high-density lipoprotein-cholesterol (LDL-C and HDL-C), triglyceride (TG), fecal TC, and hepatic TC, lipid peroxide (LPO) and proinflammatory TNFα levels were determined. Relative to WR, GBR contained higher amounts of total polyphenols, total flavonoids, ß-carotene and lycopene, and exhibited a stronger in vitro DPPH-free radical scavenging ability and antihemolytic potentials. Levels of plasma TC, LDL-C, TG, and hepatic TC and TG significantly decreased, while plasma HDL-C and fecal TC levels significantly increased in the GBR-fed HC-rats, indicating dietary GBR demonstrates a stronger antilipidemic effect than WR. The hepatic levels of LPO and TNFα also decreased (p<0.05) to a greater extent in GBR-fed HC-rats than those in the WR-fed rats. It is thus concluded that dietary GBR could be a natural treatment of hypercholesterolemia and related cardiovascular risk factors, and a source of antioxidants to reduce hemolysis and related anemia.

Rice Germosprout Extract Protects Erythrocytes from Hemolysis and the Aorta, Brain, Heart, and Liver Tissues from Oxidative Stress In Vitro.

Identifying dietary alternatives for artificial antioxidants capable of boosting antihemolytic and antioxidative defense has been an important endeavor in improving human health. In the present study, we studied antihemolytic and antioxidative effects of germosprout (i.e., the germ part along with sprouted stems plus roots) extract prepared from the pregerminated rice. The extract contained considerable amounts of antioxidant ß-carotene (414 ± 12 ng/g of extract) and phytochemicals such as total polyphenols (12.0 ± 1.1 mg gallic acid equivalent/g of extract) and flavonoids (11.0 ± 1.4 mg catechin equivalent/g of extract). The antioxidant potential of the extract was assessed by its DPPH- (2,2-diphenyl-1-picrylhydrazyl-) free radical scavenging activity where we observed that germosprout extract had considerable antioxidative potentials. To evaluate antihemolytic effect of the extract, freshly prepared erythrocytes were incubated with either peroxynitrite or Fenton's reagent in the absence or presence of the extract. We observed that erythrocytes pretreated with the extract exhibited reduced degree of in vitro hemolysis. To support the proposition that germosprout extract could act as a good antioxidative agent, we also induced in vitro oxidative stress in erythrocyte membranes and in the aorta, brain, heart, and liver tissue homogenates in the presence of the extract. As expected, germosprout extract decreased oxidative stress almost to the same extent as that of vitamin E, as measured by lipid peroxide levels, in all the mentioned tissues. We conclude that rice germosprout extract could be a good natural source of antioxidants to reduce oxidative stress-induced hemolysis and damage of blood vessels and other tissues.