RESUMO
The nuclear accident occurred at Fukushima Dai-ichi Nuclear Power Plant (NPP) (March 11, 2011) similarly to the accident at the Chernobyl NPP (April 26, 1986) is related to the level 7 of the INES. It is of interest to make an analysis of the radionuclide composition of the fallout following the both accidents. The results of the spectrometric measurements were used in that comparative analysis. Two areas following the Chernobyl accident were considered: (1) the near zone of the fallout - the Belarusian part of the central spot extended up to 60 km around the Chernobyl NPS and (2) the far zone of the fallout--the "Gomel-Mogilev" spot centered 200 km to the north-northeast of the damaged reactor. In the case of Fukushima accident the near zone up to about 60 km considered. The comparative analysis has been done with respect to refractory radionuclides (95Zr, 95Nb, 141Ce, 144Ce), as well as to the intermediate and volatile radionuclides 103Ru, 106Ru, 131I, 134Cs, 137Cs, 140La, 140Ba and the results of such a comparison have been discussed. With respect to exposure to the public the most important radionuclides are 131I and 137Cs. For the both accidents the ratios of 131I/137Cs in the considered soil samples are in the similar ranges: (3-50) for the Chernobyl samples and (5-70) for the Fukushima samples. Similarly to the Chernobyl accident a clear tendency that the ratio of 131I/137Cs in the fallout decreases with the increase of the ground deposition density of 137Cs within the trace related to a radioactive cloud has been identified for the Fukushima accident. It looks like this is a universal tendency for the ratio of 131I/137Cs versus the 137Cs ground deposition density in the fallout along the trace of a radioactive cloud as a result of a heavy accident at the NPP with radionuclides releases into the environment. This tendency is important for an objective reconstruction of 131I fallout based on the results of 137Cs measurements of soil samples carried out at late dates after the Fukushima accident.
Assuntos
Radioisótopos de Césio/análise , Acidente Nuclear de Chernobyl , Acidente Nuclear de Fukushima , Radioisótopos do Iodo/análise , Cinza Radioativa/análise , Humanos , Japão , Centrais Nucleares , Doses de Radiação , Monitoramento de Radiação/métodos , Poluentes Radioativos do Solo/análise , Espectrometria gama , U.R.S.S.RESUMO
Nocturnal polyuria is the most frequent cause of nocturia, a common disease associated with a compromised quality of life and increased mortality. Its pathogenesis is complex, and the detailed underlying mechanism remains unknown. Herein, we report that concomitant intake of a high-salt diet and reduced nitric oxide (NO) production achieved through Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME) administration in mice resulted in nocturnal polyuria recapitulating the clinical features in humans. High salt intake under reduced NO production overactivated the angiotensin II-SPAK (STE20/SPS1-related proline-alanine-rich protein kinase)-NCC (sodium chloride co-transporter) pathway in the kidney, resulting in the insufficient excretion of sodium during the day and its excessive excretion at night. Excessive Na excretion at night in turn leads to nocturnal polyuria due to osmotic diuresis. Our study identified a central role for the intrarenal angiotensin II-SPAK-NCC pathway in the pathophysiology of nocturnal polyuria, highlighting its potential as a promising therapeutic target.
Assuntos
Angiotensina II , Noctúria , Angiotensina II/metabolismo , Angiotensina II/farmacologia , Animais , Camundongos , Camundongos Knockout , Óxido Nítrico , Fosforilação , Poliúria/etiologia , Proteínas Serina-Treonina Quinases , Qualidade de Vida , Cloreto de Sódio na Dieta/efeitos adversosRESUMO
In a previous paper, Takamiya et al. calculated (63)Ni production in copper samples exposed to the Hiroshima atomic bomb. More specifically, they used their experimental cross-section values of the (63)Cu(n,p)(63)Ni reaction and compared the result with that of the corresponding calculation in the radiation dosimetry system DS02, which used another set of cross-section values. These results were different, and the following two reasons were found: typographical errors in several energy boundary values in the DS02 report that was also used in the calculation by Takamiya et al. and an inappropriate assumption on the cross-section values of the low neutron energy region in the calculation by Takamiya et al. These two issues are described and amended in the present report.
Assuntos
Cobre/análise , Níquel/análise , Monitoramento de Radiação/métodos , Radioisótopos/análise , Japão , Nêutrons , Guerra Nuclear , Armas Nucleares , RadiometriaRESUMO
The first measurements of (63)Ni produced by A-bomb fast neutrons (above approximately 1 MeV) in copper samples from Hiroshima encompassed distances from approximately 380 to 5062 m from the hypocenter (the point on the ground directly under the bomb). They included the region of interest to survivor studies (approximately 900 to 1500 m) and provided the first direct validation of fast neutrons in that range. However, a significant measurement gap remained between the hypocenter and 380 m. Measurements close to the hypocenter are important as a high-value anchor for the slope of the curve for neutron activation as a function of distance. Here we report measurements of (63)Ni in copper samples from the historic Hiroshima Atomic Bomb Dome, which is located approximately 150 m from the hypocenter. These measurements extend the range of our previously published data for (63)Ni providing a more comprehensive and consistent A-bomb activation curve. The results are also in good agreement with calculations based on the current dosimetry system (DS02) and give further experimental support to the accuracy of this system that forms the basis for radiation risk estimates worldwide.
Assuntos
Cobre/química , Nêutrons Rápidos , Armas Nucleares , Japão , Espectrometria de Massas , Níquel/análise , Radioisótopos/análiseRESUMO
An efficient system for the import of newly synthesized proteins into highly purified rat liver peroxisomes was reconstituted in vitro. 35S-Labeled acyl-CoA oxidase (AOx) was incorporated into peroxisomes in a proteinase K-resistant fashion. This import was specific (did not occur with mitochondria) and was dependent on temperature, time, and peroxisome concentration. Under optimal conditions approximately 30% of [35S]AOx became proteinase resistant. The import of AOx into peroxisomes could be dissociated into two steps: (a) binding occurred at 0 degrees C in the absence of ATP; (b) translocation occurred only at 26 degrees C and required the hydrolysis of ATP. GTP would not substitute for ATP and translocation was not inhibited by carbonylcyanide-m-chlorophenylhydrazone, valinomycin, or other ionophores.
Assuntos
Microcorpos/metabolismo , Oxirredutases/metabolismo , Acil-CoA Oxidase , Trifosfato de Adenosina/metabolismo , Transporte Biológico Ativo , Carbonil Cianeto m-Clorofenil Hidrazona/farmacologia , Fracionamento Celular , Centrifugação com Gradiente de Concentração , Técnicas In Vitro , Iohexol , Ionóforos/farmacologia , Cinética , Magnésio/farmacologia , Cloreto de Magnésio , Potenciais da Membrana , Cloreto de Potássio/farmacologia , TemperaturaRESUMO
Peroxisomes are apparently missing in Zellweger syndrome; nevertheless, some of the integral membrane proteins of the organelle are present. Their distribution was studied by immunofluorescence microscopy. In control fibroblasts, peroxisomes appeared as small dots. In Zellweger fibroblasts, the peroxisomal membrane proteins were located in unusual empty membrane structures of larger size. These results suggest that the primary defect in this disease may be in the mechanism for import of matrix proteins.
Assuntos
Doenças Genéticas Inatas/patologia , Proteínas de Membrana/análise , Microcorpos/patologia , Fibroblastos/análise , Fibroblastos/ultraestrutura , Imunofluorescência , Doenças Genéticas Inatas/metabolismo , Humanos , Membranas Intracelulares/análise , Membranas Intracelulares/patologia , Microcorpos/análise , Organoides/análise , Organoides/patologia , SíndromeRESUMO
Recently, we reported that baicalein 5,6,7-trimethyl ether (BTM), a flavonoid, is capable of activating fatty acid beta-oxidation in X-linked adrenoleukodystrophy (X-ALD) fibroblasts (FEBS Lett. 2005; 579: 409-414). The objective of this study was to clarify whether BTM activates peroxisomal and/or mitochondrial fatty acid beta-oxidation. We first analysed the effect of BTM on fatty acid beta-oxidation in fibroblasts derived from healthy controls as well as patients with X-ALD, mitochondrial carnitine-acylcarnitine translocase (CACT) deficiency, and peroxisome biogenesis disorder, Zellweger syndrome. Lignoceric acid (C(24:0)) beta-oxidation in the fibroblasts was stimulated by treatment with BTM, except for Zellweger fibroblasts. In contrasts, palmitic acid (C(16:0)) beta-oxidation was increased (2.8-fold) only in CACT-deficient fibroblasts. In U87 glioblastoma cells, C(24:0) beta-oxidation was also activated by treatment with BTM but C(16:0) beta-oxidation was not. The C(16:0) beta-oxidation was, however, significantly increased in the presence of 2-[5-(4-chlorophenyl)pentyl]oxirane-2-carboxylate (POCA), a carnitine palmitoyltransferase I inhibitor. These results indicate that BTM activates peroxisomal but not mitochondrial fatty acid beta-oxidation. In addition, we found that BTM did not upregulate the expression of ABCD2/ALDR, ABCD3/PMP70, ACOX1 and FATP4 genes but slightly increased ACSVL1 gene expression.
Assuntos
Ácidos Graxos/metabolismo , Flavanonas/farmacologia , Mitocôndrias/efeitos dos fármacos , Peroxissomos/efeitos dos fármacos , Subfamília D de Transportador de Cassetes de Ligação de ATP , Transportadores de Cassetes de Ligação de ATP/genética , Adrenoleucodistrofia/metabolismo , Carnitina Aciltransferases/deficiência , Células Cultivadas , Coenzima A Ligases/genética , Compostos de Epóxi/farmacologia , Fibroblastos/metabolismo , Glioblastoma/metabolismo , Humanos , Mitocôndrias/metabolismo , Oxirredução , Peroxissomos/metabolismo , Síndrome de Zellweger/metabolismoRESUMO
The village of Dolon located about 60 km northeast from the border of the Semipalatinsk Nuclear Test Site in Kazakhstan is one of the most affected inhabited settlements as a result of nuclear tests by the former USSR. Radioactive contamination in Dolon was mainly caused by the first USSR nuclear test on 29 August 1949. As part of the efforts to reconstruct the radiation dose in Dolon, Cs and Pu in soil samples collected from 26 locations in the vicinity of and within the village were measured to determine the width and position of the center-axis of the radioactive plume that passed over the village from the 29 August 1949 nuclear test. Measured soil inventories of Cs and Pu were plotted as a function of the distance from the supposed center-axis of the plume. A clear shape similar to a Gaussian function was observed in their spatial distributions with each maximum around a center-axis. It was suggested that the plume width that contaminated Dolon was at most 10 km and the real center-axis of the radioactive plume passed 0.7-0.9 km north of the supposed centerline. A peak-like shape with the maximum near the center-axis was also observed in the spatial distribution of the Pu/Cs activity ratio, which may reflect the fractionation effect between Pu and Cs during the deposition process. These results support the recently reported results. The data obtained here will provide useful information on the efforts to estimate radiation dose in Dolon as reliably as possible. Health Phys. 94(4):328-337; 2008.
Assuntos
Poluentes Radioativos do Ar/análise , Radioisótopos de Césio/análise , Plutônio/análise , Cinza Radioativa/análise , Poluentes Radioativos do Solo/análise , Exposição Ambiental/efeitos adversos , Cazaquistão , RadioisótoposRESUMO
Newly synthesized peroxisomal proteins enter preexisting peroxisomes posttranslationally in vivo, generally without proteolytic processing. An efficient reconstitution of this process in vitro together with cloned DNAs for peroxisomal proteins would make possible investigation of the molecular information that targets proteins to peroxisomes. We have previously reported the isolation of clones for Candida tropicalis peroxisomal proteins; here we describe the association (and possible import) of peroxisomal proteins with peroxisomes in vitro. C. tropicalis was grown in a medium containing Brij 35, resulting in the induction of a moderate number of medium-sized peroxisomes. These peroxisomes, isolated in a sucrose gradient, had a catalase latency of 54% and were sufficiently stable to be concentrated and used in an import assay. The reticulocyte lysate translation products of total RNA from oleate-grown cells were incubated with the peroxisomes at 26 degrees C in the presence of 50 mM KCl, protease inhibitors, 0.5 M sucrose, 2.5 mM MOPS (morpholinepropanesulfonic acid) (pH 7.2), and 0.5 mM EDTA. Ten major translation products (which could be immunoprecipitated with antiserum against peroxisomal protein) became progressively associated with the peroxisomes during the first 30 min of incubation (some up to approximately 70%). These include acyl coenzyme A oxidase and the trifunctional protein hydratase-dehydrogenase-epimerase. This association did not occur at 4 degrees C nor did it occur if the peroxisomes were replaced with mitochondria.
Assuntos
Candida/fisiologia , Catalase/metabolismo , Compartimento Celular , Microcorpos/fisiologia , Oxirredutases/metabolismo , Acil-CoA Oxidase , Transporte Biológico Ativo , Fracionamento Celular , Sistema Livre de Células , Clonagem Molecular , Microscopia Eletrônica , Cloreto de Potássio/farmacologia , Processamento de Proteína Pós-Traducional , TemperaturaRESUMO
In order to resolve the discrepancy between the measured and calculated 152Eu activity induced by the atomic bomb at Hiroshima, extremely low background gamma-ray spectrometry was performed for 17 granite samples collected from 134 m to more than 3 km from the hypocenter. Measurements agreed well with theoretical calculations based on DS02 up to 1.4 km from hypocenter.
Assuntos
Algoritmos , Európio/análise , Substâncias Explosivas/efeitos adversos , Nêutrons Rápidos/efeitos adversos , Guerra Nuclear , Dióxido de Silício/análise , Japão , Doses de Radiação , Espectrometria gama/métodosRESUMO
BACKGROUND: Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) is the key enzyme of the Calvin-Benson cycle and catalyzes the primary reaction of CO2 fixation in plants, algae, and bacteria. Rubiscos have been so far classified into two types. Type I is composed of eight large subunits (L subunits) and eight small subunits (S subunits) with tetragonal symmetry (L8S8), but type II is usually composed only of two L subunits (L2). Recently, some genuinely active Rubiscos of unknown physiological function have been reported from archaea. RESULTS: The crystal structure of Rubisco from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 (Tk-Rubisco) was determined at 2.8 A resolution. The enzyme is composed only of L subunits and showed a novel (L2)5 decameric structure. Compared to previously known type I enzymes, each L2 dimer is inclined approximately 16 degrees to form a toroid-shaped decamer with its unique L2-L2 interfaces. Differential scanning calorimetry (DSC), circular dichroism (CD), and gel permeation chromatography (GPC) showed that Tk-Rubisco maintains its secondary structure and decameric assembly even at high temperatures. CONCLUSIONS: The present study provides the first structure of an archaeal Rubisco, an unprecedented (L2)5 decamer. Biochemical studies indicate that Tk-Rubisco maintains its decameric structure at high temperatures. The structure is distinct from type I and type II Rubiscos and strongly supports that Tk-Rubisco should be classified as a novel type III Rubisco.
Assuntos
Ribulose-Bifosfato Carboxilase/química , Thermococcus/enzimologia , Sequência de Aminoácidos , Varredura Diferencial de Calorimetria , Cromatografia em Gel , Dicroísmo Circular , Cristalografia por Raios X , Íons , Modelos Moleculares , Dados de Sequência Molecular , Conformação Proteica , Homologia de Sequência de AminoácidosRESUMO
Human hepaotoblastoma cells (Hep G2) were cultured with a competitive inhibitor of HMG-CoA reductase, CS-514. The synthesis of cholesterol was markedly inhibited after 1 h preincubation with CS-514. The synthesis and secretion of apolipoprotein (apo) B and A-1, however, were not affected. A long-term incubation (21-24 h) of cells with CS-514 did not change apo B synthesis and secretion, although a slight depression of apo A-1 synthesis was observed. Hep G2 cells were found to secrete LDL- and HDL-like lipoproteins which were poor in cholesterol when cells were incubated with the drug. These results suggest that the modulation of cholesterol synthesis affects neither the synthesis and secretion of apo B and A-1 nor the formation of lipoproteins.
Assuntos
Apolipoproteínas A/biossíntese , Apolipoproteínas B/biossíntese , Carcinoma Hepatocelular/metabolismo , Ácidos Heptanoicos/farmacologia , Inibidores de Hidroximetilglutaril-CoA Redutases , Naftalenos/farmacologia , Apolipoproteína A-I , Apolipoproteínas A/metabolismo , Apolipoproteínas B/metabolismo , Colesterol/metabolismo , Ensaio de Imunoadsorção Enzimática , Humanos , Lipoproteínas/metabolismo , Neoplasias Hepáticas , Pravastatina , Células Tumorais Cultivadas/efeitos dos fármacos , Células Tumorais Cultivadas/metabolismo , UltracentrifugaçãoRESUMO
Arthrofactin (AF) and surfactin (SF) are the most effective cyclic lipopeptide biosurfactants ever reported. Linear AF and linear SF were prepared by saponification of lactone ring. The oil displacement activities decreased to one third of their respective original values. When residues of both an aspartic acid and a glutamic acid of SF were methylated or amidated, the activity increased by 20%, although their water solubility was lost. When these amino acid residues were modified by aminomethane sulfonic acid, the activity was drastically decreased probably owing to charge repulsion and structural distortion inhibiting micelle formation. Both AF and SF expressed higher activity under alkaline conditions than acidic conditions. AF was more resistant to acidic conditions than SF and it kept high activity even under pH 0.5. Although SF drastically reduced its activity under acidic conditions, surfactin-Asp/Glu-amido ester and surfactin-Asp/Glu-methyl ester retained similar activities irrespective of the pH change. A couple of conformers of SF prepared by reverse-phase HPLC showed the same oil displacement activity but different surface tension-reducing activity. AF was produced as a series of different fatty acid chain lengths (from C8 to C12). Among them, AF with fatty acid chain length of C10, which was the main product of the strain, showed the highest activity.
Assuntos
Lipoproteínas/química , Tensoativos/química , Proteínas de Bactérias/química , Ácidos Graxos/química , Concentração de Íons de Hidrogênio , Lactonas/química , Lipopeptídeos , Peptídeos Cíclicos/química , Isoformas de Proteínas/química , Relação Estrutura-Atividade , Tensão Superficial , Tensoativos/síntese químicaRESUMO
The inhibitory effect of suramin on the import of [35S]acyl-CoA oxidase into purified rat liver peroxisomes was investigated in vitro. The import of acyl-CoA oxidase was inhibited completely by 10 microM suramin, whilst the latency of catalase remained unchanged. The important value decreased 60% by pretreatment of peroxisomes with 10 microM suramin, but it did not decrease by pretreatment of translation products. Polysulfonate compounds which have two clusters of negative charges, such as Cibacron blue F3GA and Trypan blue, as well as suramin, inhibited the import, whilst mono- and disulfonate compounds did not.
Assuntos
Fígado/enzimologia , Microcorpos/efeitos dos fármacos , Oxirredutases/metabolismo , Suramina/farmacologia , Acil-CoA Oxidase , Adenosina Trifosfatases/efeitos dos fármacos , Sequência de Aminoácidos , Animais , Transporte Biológico/efeitos dos fármacos , Catalase/metabolismo , Microcorpos/enzimologia , Dados de Sequência Molecular , RatosRESUMO
BALB/c mice were immunized with peroxisomal membranes prepared from rat liver. Spleen cells were fused with myeloma cells (P3/U1) and the hybridomas were selected using peroxisomal membranes. A monoclonal antibody (PXM1a/207B) which recognized peroxisomal membranes was selected. Using the antibody, a novel 57 kDa polypeptide was identified in the peroxisomal membrane fraction. Immunoblot analysis of the subcellular fractions demonstrated that the 57 kDa polypeptide was present exclusively in peroxisomal membranes. The 57 kDa polypeptide was partially digested by trypsin and chymotrypsin under conditions where peroxisomal particles remained intact, indicating that the polypeptide is exposed to the cytosolic face of the peroxisomal membrane. The amount of 57 kDa polypeptide increased in parallel with proliferation of peroxisomes by administration of clofibrate.
Assuntos
Membranas Intracelulares/química , Proteínas de Membrana/análise , Microcorpos/química , Animais , Anticorpos Monoclonais , Fracionamento Celular/métodos , Feminino , Immunoblotting , Membranas Intracelulares/imunologia , Membranas Intracelulares/metabolismo , Fígado/química , Fígado/ultraestrutura , Proteínas de Membrana/biossíntese , Proteínas de Membrana/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Microcorpos/imunologia , Microcorpos/metabolismo , Peso Molecular , RatosRESUMO
An acid lipase was purified from rabbit liver lysosomes by, in sequence, osmotic treatment of the lysosomal fraction, Sephadex LH-20, DEAE-Sephadex A-50, Bio-Gel A-5m, hydroxyapatite and, finally, Sephadex G-200 column chromatography. The substrate was 4-methylumbelliferyl oleate. The enzyme was solubilized by Sephadex LH-20 column chromatography instead of detergents and organic solvents, to obtain an intrinsic macromolecule. 4-Methylumbelliferyl oleate hydrolase, osmotically released from lysosomal particles, had a very high molecular weight (greater than 800 000) which was reduced by gel filtration on a Sephadex LH-20 column; the final molecular weight of the purified enzyme was 58 000. The specific activity of 4-methylumbelliferyl oleate hydrolase increased at almost the same rate as acid cholesterol esterase and triacylglycerol lipase after Sephadex LH-20 column chromatography; the thermal stability of the activity of the three enzymes was almost identical. We also discuss the properties of the enzyme molecule and the interaction between the enzyme and the lysosomal membrane.
Assuntos
Lipase/isolamento & purificação , Fígado/enzimologia , Animais , Feminino , Concentração de Íons de Hidrogênio , Himecromona/análogos & derivados , Himecromona/metabolismo , Cinética , Lipase/metabolismo , Lisossomos/enzimologia , Peso Molecular , Coelhos , Esterol Esterase/metabolismoRESUMO
Trifluoperazine, a calmodulin antagonist, suppressed the clofibric acid-evoked induction of the peroxisomal cyanide-insensitive fatty acyl-CoA oxidizing system and carnitine acetyltransferase in rat liver and also in cultured rat hepatocytes. H-7, a potent inhibitor of protein kinase C, also suppressed the induction of these enzymes by clofibric acid, bezafibrate, Wyl4,643 or mono(2-ethylhexyl)phthalate in cultured rat hepatocytes. This suppressive effect was also confirmed by the protein composition of hepatocytes treated with clofibric acid and these antagonists, where the increase in the amount of peroxisomal bifunctional enzyme by peroxisome proliferator was markedly suppressed by above two antagonists. Profile of the time-dependent changes in the activities of the two enzymes after clofibric acid treatment showed that there might be two phases in the induction process. The initial phase (0-3 days after the treatment) showed a relative low inducing rate and subsequent phase (3-5 days after the treatment) showed an abrupt induction. The suppressive effect of the above two antagonists was significant in the later phase. In a time course study of the induction process of peroxisomal catalase, bifunctional enzyme or 69 kDa integral membrane protein using immunochemical detection, the induction of the membrane protein by clofibric acid was delayed compared with that of the bifunctional enzyme, where the induction was inhibited almost completely by nicardipine. These experimental results suggest that calmodulin- and protein kinase C-dependent processes play an important role in the process of marked induction of peroxisomal enzymes and membrane protein by drugs in rat liver.
Assuntos
Bezafibrato/farmacologia , Calmodulina/metabolismo , Catalase/metabolismo , Clofibrato/farmacologia , Dietilexilftalato/análogos & derivados , Fígado/metabolismo , Proteínas de Membrana/metabolismo , Microcorpos/metabolismo , Proteína Quinase C/metabolismo , Pirimidinas/farmacologia , Trifluoperazina/farmacologia , 1-(5-Isoquinolinasulfonil)-2-Metilpiperazina , Animais , Peso Corporal/efeitos dos fármacos , Calmodulina/antagonistas & inibidores , Células Cultivadas , Dietilexilftalato/farmacologia , Eletroforese em Gel de Poliacrilamida , Isoquinolinas/farmacologia , Cinética , Fígado/efeitos dos fármacos , Fígado/enzimologia , Masculino , Proteínas de Membrana/isolamento & purificação , Microcorpos/efeitos dos fármacos , Microcorpos/enzimologia , Peso Molecular , Tamanho do Órgão/efeitos dos fármacos , Oxirredução , Piperazinas/farmacologia , Proteína Quinase C/antagonistas & inibidores , Ratos , Ratos Endogâmicos , Valores de ReferênciaRESUMO
Determination of the nucleotide sequence of the gene encoding a lipase from Pseudomonas sp. MIS38 (PML) revealed that PML is a member of the lipase family I.3 and is composed of 617 amino acid residues with a calculated molecular weight of 64510. Recombinant PML (rPML) was overproduced in Escherichia coli in an insoluble form, solubilized in the presence of 8 M urea, purified in a urea-denatured form and refolded by removing urea in the presence of the Ca(2+) ion. Gel filtration chromatography suggests that this refolded protein is monomeric. rPML showed relatively broad substrate specificities and hydrolyzed glyceryl tributyrate and olive oil with comparable efficiencies. rPML was active only in the form of a holo-enzyme, in which at least 12 Ca(2+) ions bound. These Ca(2+) ions bound too tightly to be removed from the protein upon dialysis, but were removed from it upon EDTA treatment. The resultant apo-enzyme was fully active in the presence of 10 mM CaCl(2), but was inactive in the absence of the Ca(2+) ion. PML has a GXSXG motif, which is conserved in lipases/esterases and generally contains the active-site serine. The mutation of Ser(207) within this motif to Ala completely inactivated PML, suggesting that Ser(207) is the active-site serine of PML.
Assuntos
Proteínas de Bactérias/genética , Escherichia coli/metabolismo , Lipase/genética , Pseudomonas/genética , Sequência de Aminoácidos , Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/química , Cálcio/química , Escherichia coli/genética , Concentração de Íons de Hidrogênio , Lipase/biossíntese , Lipase/química , Dados de Sequência Molecular , Mutação , Pseudomonas/enzimologia , Alinhamento de Sequência , TemperaturaRESUMO
A new monoclonal antibody against malondialdehyde (MDA)-treated low density lipoprotein (LDL) was raised using homogenate of human atheroma as immunogen. This antibody, DLH2, was obtained by selecting the clones which did not react to native LDL but did react to copper-induced oxidized LDL (OxLDL). DLH2 showed a greater reactivity to MDA-LDL than to OxLDL. When LDL was treated with various aldehyde containing reagents, treatment of LDL with glutaraldehyde or MDA greatly increased the reactivity to the antibody, while LDL treated with 2,4-hexadienal or 4-hydroxynonenal was not reactive. Among many proteins tested, high density lipoprotein, bovine serum albumin and hemoglobin showed significant reactivity to DLH2 after they were treated with MDA or glutaraldehyde. When low density and high density lipoproteins treated with MDA were subjected to immunoblot analysis, newly formed products larger than the original apolipoproteins were detected with the antibody, suggesting that this antibody recognizes aggregated proteins with divalent short chain cross linkers. The antigenic materials were shown by immunohistochemical analysis to be present in foamy macrophages in human atheromatous lesions. DLH2 antigen did not colocalize either with apolipoprotein B. Furthermore, we found a massive accumulation of the antigenic material in Kupffer cells in the liver of rats treated with alcohol and carbonyl iron, a model of hepatic fibrosis due to oxidative stress. These results suggest the presence of cross linked proteins in damaged tissues.
Assuntos
Anticorpos Monoclonais/imunologia , Lipoproteínas LDL/imunologia , Cirrose Hepática Experimental/imunologia , Animais , Especificidade de Anticorpos , Bovinos , Reações Cruzadas , Humanos , Imuno-Histoquímica , Peroxidação de Lipídeos , Fígado/imunologia , Fígado/patologia , Masculino , Malondialdeído , Ratos , Ratos WistarRESUMO
To examine the prevalence of abnormalities in the insulin receptor structure gene in Japanese with non-insulin-dependent diabetes mellitus (NIDDM), a population of 51 patients with NIDDM was screened for mutations in this gene. Patient genomic DNAs of both alleles corresponding to 22 exons of the gene were amplified by polymerase chain reaction (PCR). The PCR products on pUC19 were sequenced. Three patients with heterozygous missense mutation Thr831-->Ala831 in exon 13 and one patient with heterozygous missense mutation Tyr1334-->Cys1334 in exon 22 of the beta-subunits were identified. Linkage analysis of one of the families plus statistical studies showed that the mutation Thr831-->Ala831 is possibly responsible for the onset of NIDDM. In COS cells transiently expressing both mutant receptor cDNAs and a cDNA of a M(r) 85,000 regulatory subunit of phosphatidylinositol 3-kinase (PI 3-kinase), the mutation Tyr1334-->Cys1334 impaired binding of the receptor with the M(r) 85,000 subunit of PI 3-kinase, but linkage analysis of the family showed that the mutation did not cosegregate with NIDDM in the pedigree. Therefore, one missense mutation (Thr831-->Ala831) in the insulin receptor, as found in three patients, is possibly involved in the etiology of a subset of the 51 NIDDM patients.