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1.
Dev Dyn ; 249(5): 622-635, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-31900962

RESUMO

BACKGROUND: Splicing factor 3B subunit 4 (SF3B4) is a causative gene of an acrofacial dysostosis, Nager syndrome. Although in vitro analyses of SF3B4 have proposed multiple noncanonical functions unrelated to splicing, less information is available based on in vivo studies using model animals. RESULTS: We performed expression and functional analyses of Sf3b4 in mice. The mouse Sf3b4 transcripts were found from two-cell stage, and were ubiquitously present during embryogenesis with high expression levels in several tissues such as forming craniofacial bones and brain. In contrast, expression of a pseudogene-like sequence of mouse Sf3b4 (Sf3b4_ps) found by in silico survey was not detected up to embryonic day 10. We generated a Sf3b4 knockout mouse using CRISPR-Cas9 system. The homozygous mutant mouse of Sf3b4 was embryonic lethal. The heterozygous mutant of Sf3b4 mouse (Sf3b4+/- ) exhibited smaller body size compared to the wild-type from postnatal to adult period, as well as homeotic posteriorization of the vertebral morphology and flattened calvaria. The flattened calvaria appears to be attributable to mild microcephaly due to a lower cell proliferation rate in the forebrain. CONCLUSIONS: Our study suggests that Sf3b4 controls anterior-posterior patterning of the axial skeleton and guarantees cell proliferation for forebrain development in mice.


Assuntos
Prosencéfalo/metabolismo , Esqueleto/metabolismo , Animais , Feminino , Masculino , Camundongos , Mutação/genética , Fatores de Processamento de RNA/genética , Fatores de Processamento de RNA/metabolismo
2.
BMC Genomics ; 19(1): 318, 2018 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-29720086

RESUMO

BACKGROUND: CRISPR/Cas9 enables the targeting of genes in zygotes; however, efficient approaches to create loxP-flanked (floxed) alleles remain elusive. RESULTS: Here, we show that the electroporation of Cas9, two gRNAs, and long single-stranded DNA (lssDNA) into zygotes, termed CLICK (CRISPR with lssDNA inducing conditional knockout alleles), enables the quick generation of floxed alleles in mice and rats. CONCLUSIONS: The high efficiency of CLICK provides homozygous knock-ins in oocytes carrying tissue-specific Cre, which allows the one-step generation of conditional knockouts in founder (F0) mice.


Assuntos
Engenharia Genética/métodos , Alelos , Animais , Sequência de Bases , Sistemas CRISPR-Cas/genética , Injeções , Camundongos , Camundongos Knockout , Zigoto/metabolismo
3.
BMC Genomics ; 17(1): 979, 2016 11 28.
Artigo em Inglês | MEDLINE | ID: mdl-27894274

RESUMO

BACKGROUND: Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing long homology arms is a laborious process for high-throughput knock-in. We recently developed the CRISPR/Cas-based precise integration into the target chromosome (PITCh) system for a gene cassette knock-in without long homology arms mediated by microhomology-mediated end-joining. RESULTS: Here, we identified exonuclease 1 (Exo1) as an enhancer for PITCh in human cells. By combining the Exo1 and PITCh-directed donor vectors, we achieved convenient one-step knock-in of gene cassettes and floxed allele both in human cells and mouse zygotes. CONCLUSIONS: Our results provide a technical platform for high-throughput knock-in.


Assuntos
Técnicas de Introdução de Genes , Recombinação Homóloga , Zigoto , Animais , Sequência de Bases , Sistemas CRISPR-Cas , Linhagem Celular , Cromossomos , Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas , Exodesoxirribonucleases/metabolismo , Marcação de Genes , Loci Gênicos , Humanos , Camundongos , Nucleases dos Efetores Semelhantes a Ativadores de Transcrição
4.
Genome Biol ; 16: 87, 2015 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-25924609

RESUMO

Although the CRISPR/Cas system has enabled one-step generation of knockout mice, low success rates of cassette knock-in limit its application range. Here we show that cloning-free, direct nuclear delivery of Cas9 protein complex with chemically synthesized dual RNAs enables highly efficient target digestion, leading to generation of knock-in mice carrying a functional cassette with up to 50% efficiency, compared with just 10% by a commonly used method consisting of Cas9 mRNA and single guide RNA. Our cloning-free CRISPR/Cas system facilitates rapid one-step generation of cassette knock-in mice, accelerating functional genomic research by providing various in vivo genetic tools.


Assuntos
Sistemas CRISPR-Cas/genética , Clonagem Molecular , Técnicas de Introdução de Genes , Animais , Linhagem Celular , Feminino , Marcação de Genes , Genes Reporter , Loci Gênicos , Células HEK293 , Humanos , Masculino , Camundongos , Camundongos Knockout , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Análise de Sequência de DNA
5.
PLoS One ; 8(10): e75959, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24098411

RESUMO

In rice (Oryza sativa L.), there is a diversity in flowering time that is strictly genetically regulated. Some indica cultivars show extremely late flowering under long-day conditions, but little is known about the gene(s) involved. Here, we demonstrate that functional defects in the florigen gene RFT1 are the main cause of late flowering in an indica cultivar, Nona Bokra. Mapping and complementation studies revealed that sequence polymorphisms in the RFT1 regulatory and coding regions are likely to cause late flowering under long-day conditions. We detected polymorphisms in the promoter region that lead to reduced expression levels of RFT1. We also identified an amino acid substitution (E105K) that leads to a functional defect in Nona Bokra RFT1. Sequencing of the RFT1 region in rice accessions from a global collection showed that the E105K mutation is found only in indica, and indicated a strong association between the RFT1 haplotype and extremely late flowering in a functional Hd1 background. Furthermore, SNPs in the regulatory region of RFT1 and the E105K substitution in 1,397 accessions show strong linkage disequilibrium with a flowering time-associated SNP. Although the defective E105K allele of RFT1 (but not of another florigen gene, Hd3a) is found in many cultivars, relative rate tests revealed no evidence for differential rate of evolution of these genes. The ratios of nonsynonymous to synonymous substitutions suggest that the E105K mutation resulting in the defect in RFT1 occurred relatively recently. These findings indicate that natural mutations in RFT1 provide flowering time divergence under long-day conditions.


Assuntos
Flores/crescimento & desenvolvimento , Mutação , Oryza/crescimento & desenvolvimento , Oryza/genética , Proteínas de Plantas/genética , Sequência de Aminoácidos , Substituição de Aminoácidos , Cromossomos de Plantas/genética , Regulação da Expressão Gênica no Desenvolvimento , Genômica , Haplótipos , Desequilíbrio de Ligação , Dados de Sequência Molecular , Proteínas de Plantas/química , Polimorfismo de Nucleotídeo Único , Locos de Características Quantitativas/genética , Fatores de Tempo
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