RESUMO
Cutaneous Leishmaniasis is endemic in the tribal district of Khyber near the Pak-Afghan border and is caused by Leishmania tropica. In Pakistan, cutaneous leishmaniasis caused by L. tropica is considered anthroponotic and is thought to be maintained by a human-sand fly-human transmission cycle. Along with humans, other mammals may also be acting as reservoir hosts of leishmaniasis in the study area. To investigate the role of non-human mammals in the transmission of leishmaniasis, blood samples were collected from 245 animals from the CL endemic district of Khyber, Pakistan. Leishmania parasite in these samples was detected by amplifying the species-specific sequences in minicircle kinetoplast DNA, using PCR. L. tropica DNA was detected in 18 (7.35%) samples, comprising 11 cows (Bos taurus), 6 goats (Capra hircus), and 1 dog (Canus lupus familiaris). Only a single cow and dog had a leishmaniasis-like lesion, and the remaining positive samples were asymptomatic. None of the tested sheep (Ovis aries) and rat (Rattus rattus, Rattus norvegicus) was positive. The present study reports the first instance of molecular detection of L. tropica in domestic animals. Our study indicates that along with humans' cows, goats and dogs may also be playing an important role in the transmission of anthroponotic cutaneous leishmaniasis in district Khyber in particular and Pakistan in general.
Assuntos
Leishmania tropica , Leishmaniose Cutânea , Feminino , Humanos , Animais , Ratos , Bovinos , Cães , Ovinos , Leishmania tropica/genética , Paquistão/epidemiologia , Leishmaniose Cutânea/epidemiologia , Leishmaniose Cutânea/veterinária , Leishmaniose Cutânea/diagnóstico , Animais Domésticos , CabrasRESUMO
In this study, in vitro anti-leishmanial activity of buparvaquone was evaluated against promastigotes and intracellular amastigotes of Pakistani Leishmania tropica isolate KWH23 in relation to the current standard chemotherapy for leishmaniasis (sodium stibogluconate, sodium stibogluconate, amphotericin B and miltefosine). For buparvaquone, mean % inhibition in intracellular amastigotes at four different concentrations (1.35 µM, 0.51 µM, 0.17 µM and 0.057 µM) was 78%, 44%, 20% and 14% respectively, whereas, against promastigotes it was 89%, 77%, 45% and 35% respectively. IC50 values calculated to estimate the anti-leishmanial activity of buparvaquone against intra-cellular amastigotes and promastigotes was 0.53 µM (95% C.I. = 0.32-0.89) and 0.15 µM (95% C.I. = 0.01-1.84) respectively. Amphotericin B was the most potent in-vitro drug tested, with an IC50 of 0.075 µM (95% C.I. = 0.006-0.907) against promastigotes, and 0.065 µM (95% C.I. = 0.048-0.089) against intra-cellular amastigotes. Amphotericin B was more cytotoxic against THP1 cells, with an IC50 of 0.15 µM (95% C.I. = 0.01-0.95) and an apparent in-vitro therapeutic index of 2.0, than was buparvaquone, with an IC50 of 12.03 µM (95% C.I. = 5.36-26.96) against THP1 cells and a therapeutic index of 80.2. The study proposes that buparvaquone may be further investigated as a candidate drug for treatment of cutaneous leishmaniasis.
Assuntos
Antiprotozoários/farmacologia , Leishmania tropica/efeitos dos fármacos , Naftoquinonas/farmacologia , Anfotericina B/farmacologia , Anfotericina B/toxicidade , Gluconato de Antimônio e Sódio/farmacologia , Gluconato de Antimônio e Sódio/toxicidade , Antiprotozoários/toxicidade , Linhagem Celular Tumoral/efeitos dos fármacos , Criança , Humanos , Concentração Inibidora 50 , Leishmania tropica/crescimento & desenvolvimento , Leishmaniose Cutânea/tratamento farmacológico , Leishmaniose Cutânea/parasitologia , Macrófagos/parasitologia , Masculino , Meglumina/farmacologia , Meglumina/toxicidade , Antimoniato de Meglumina , Naftoquinonas/toxicidade , Compostos Organometálicos/farmacologia , Compostos Organometálicos/toxicidade , Paquistão , Testes de Sensibilidade ParasitáriaRESUMO
BACKGROUND: Oculocutaneous albinism (OCA) is a group of skin depigmentation disorders. Clinical presentation of OCA includes defects in melanocyte differentiation, melanin biosynthesis, and melanosome maturation and transport. OBJECTIVES: A molecular diagnostics study of families presenting oculocutaneous albinism. METHODS: In this study, 17 consanguineous OCA families consisting of 93 patients were investigated. Whole Exome Sequencing (WES) of the index patient in each family were performed. Short listed variants of WES were Sanger validated for Mendelian segregation in obligate carriers and other available family members. Variant prioritization and pathogenicity were classified as per the criteria of American College Medical Genetics and Genomics (ACMG). Comparative computational modelling was performed to predict the potential damaging effect of the altered proteins. RESULTS: 15 pathogenic variations: c.132 T > A, c.346C > T, c.488C > G, c.1037G > A in TYR, c.1211C > T, c.1441G > A, c.1706_1707insT, c.2020C > G, c.2402G > C, c.2430del, in OCA2, c.1067G > A in TYRP1 and c.451C > T, c.515G > T, c.766C > T, c.917G > A in MC1R genes were identified. Three variants in OCA2 gene were characterized: c.1706_1707insT, c.2430del, and c.2402G > C, all of which were not reported before in OCA families. CONCLUSION: A few studies focusing on mutation screening of OCA patients have been reported before; however, this study has uniquely presents the Pakhtun ethnic population residing on the North-Western boarder. It explains that TYR, OCA2, TYRP1, and MC1R variations lead to non-syndromic OCA phenotype The overlapping phenotypes of OCA can precisely be diagnosed for its molecular pathogenicity using WES. This study recommends WES as a first-line molecular diagnostic tool, and provides a basis for developing customized genetic tests i.e. pre-marital screening to reduce the disease burden in the future generations.
Assuntos
Albinismo Oculocutâneo , Humanos , Sequenciamento do Exoma , Albinismo Oculocutâneo/genética , Albinismo Oculocutâneo/diagnóstico , Testes Genéticos , Mutação , Proteínas de Membrana Transportadoras/genética , Glicoproteínas de Membrana/genética , Oxirredutases/genéticaRESUMO
OBJECTIVE: To observe the disease pattern of vivax malaria, and to identify the various laboratory abnormalities associated with it. METHODS: The descriptive cross-sectional study was conducted at the Department of Medicine, Abbasi Shaheed Hospital, Karachi, from July to September 2011. Clinical features and laboratory abnormalities of all patients who tested positive for Plasmodium Vivax mono-infections were collected and analysed to work out the disease pattern. SPSS 20 was used for statistical analysis. RESULTS: There were 107 patients who tested positive for vivax malaria. The most common clinical feature was fever which was present in all the 107 (100%) patients. Besides, 4 (3.7%) patients had haematemesis and 2 (1.9%) had haematuria. Thrombocytopenia was the commonest laboratory abnormality, found in 66 (61.7%) cases; 47 (43.9%) patients had significant leucopenia; between 2000-4000/cumm. One (0.93%) patient developed adult respiratory distress syndrome and expired. CONCLUSION: Atypical presentations with changing phase of severity were observed with plasmodium vivax infection. It can also lead to severe malaria, resulting in significant morbidity and mortality.
Assuntos
Epidemias/estatística & dados numéricos , Malária Vivax/epidemiologia , Plasmodium vivax/isolamento & purificação , Adolescente , Adulto , Idoso , Estudos Transversais , Diagnóstico Diferencial , Feminino , Humanos , Malária Vivax/diagnóstico , Malária Vivax/parasitologia , Masculino , Pessoa de Meia-Idade , Morbidade/tendências , Estudos Retrospectivos , Taxa de Sobrevida/tendências , Adulto JovemRESUMO
The genus Heremites Gray, 1845 is endemic to the Western Palearctic region, containing morphologically similar species with a not well resolved taxonomy. The genus has a broad distribution from North Africa to Central Asia, with the only known record from northeastern Afghanistan. Three species are currently recognized in the genus with one, H. septemtaeniatus (Reuss, 1834), representing populations at the eastern edge of the genus range. During extensive fieldwork, we discovered H. septemtaeniatus from northwestern Pakistan and provisionally suggest that this population could be morphologically defined as H. septemtaeniatus transcaucasicus (Chernov, 1926). This important contribution to the knowledge regarding the family Scincidae in Pakistan, however, needs further investigation using an integrative approach.