Vestibuloplasty by modified Kazanjian technique in treatment with dental implants.

Dentists are often faced with extensively resorbed mandibular ridges with shallow buccal vestibule and high insertion of the mentalis muscle in relation to the crest of the ridge, causing the displacement of the prosthesis. Vestibuloplasty techniques aim at eliminating the muscle insertions, reposition the mucosa, and increase the area chapeável, giving more stability to the prosthesis. Among the techniques to deepen the vestibule are submucosal vestibuloplasties by secondary epithelialization and with mucosal and skin grafts. We will discuss vestibuloplasty by secondary epithelialization with emphasis on the so-called modified Kazanjian technique. This technique provides an appropriate result and does not require hospitalization, additional surgery at the donor, or prolonged periods without the use of prosthesis.

Epidemiologic evaluation of mandibular fractures in the Rio de Janeiro high-complexity hospital.

The aim of this study was to gather data on trauma etiology and mandibular fracture localization in patients who presented at the General Hospital of Nova Iguaçu, Rio de Janeiro, Brazil. From March 2007 to December 2008, 95 patients with mandibular fracture were registered in a medical form, at the Bucomaxillofacial Surgery Department of the General Hospital of Nova Iguaçu, Rio de Janeiro, Brazil. Concerning mandibular fracture etiology, 21.05% were caused by motorcycle accidents, followed by interpersonal violence without use of weapons (punches, kicks, bumps with the head, blows with the elbow, etc) (16.84%) and interpersonal violence with firearm (14.73%). It was found that 52.63% of the patients had a single fracture line. The most affected fracture area was the parasymphysis (26.02%), followed by the condyle (22.60%) and mandibular angle (18.49%). Concerning the injury area, 24.21% were directed to the mandibular symphysis, 22.17% of the patients did not remember the injury area, and 18.94% had multiple injuries. When the injury was directed to the symphysis, the result was more condyle fractures (11.64%), and injuries at the mandibular angle resulted in fractures at the angle itself (8.90%). The most common fracture cause was traffic accidents, mainly motorcycle accidents, and the most affected areas were the parasymphysis and the condyle. The mandible isolated fractures occurred in half of the cases. Motorcycle accidents resulted in more fractures in the parasymphysis area, and when the symphysis area is affected by injuries, the result is a higher percentage in condyle fractures.

Comparative analysis of 2-flap designs for extraction of mandibular third molar.

OBJECTIVE: The objective of the study was to analyze 2-flap designs for surgical extraction of third molar, evaluating the periodontal status of the second lower molar. STUDY DESIGN: Forty-five lower third molars were extracted from 24 patients. In 23 teeth, a vertical incision to the mandibular ramus was used (technique A), whereas 22 teeth were submitted to classic L-shaped flap (technique B) with controls at 60 and 90 days postoperatively. RESULTS: Pearson correlation coefficient analysis showed a significant correlation only between immediate preoperative probing depth variables from techniques A and B in the studied surfaces. Statistical significances in the preoperative (vestibular) and postoperative day 60 (distovestibular and vestibular) were noted. In contrast, Student t-test showed no statistical difference in probing depths between preoperative and postoperative values, as well as no statistically significant difference regarding the type of incision alone. CONCLUSIONS: Technique A allowed a less traumatic surgery, guaranteeing a more comfortable postoperative period.

Sialolithiasis of the submandibular gland.

Sialolithiasis of the salivary gland is a benign pathology that occurs most frequently in the submandibular gland because of its anatomic features. Depending on the sialolith size and calcification degree, it can be visible in radiographic examinations. Commonly, patients may experience pain and/or edema, when the ducts are obstructed. The authors report the case of sialolithiasis of the submandibular gland in a 42-year-old, female, white-skinned patient, noticed during routine dental examination. Following diagnosis confirmed by clinical and radiographic examinations, the treatment plan consisted of surgery for removal of the calcified mass. The prognosis is often good, and generally there is no recurrence.

Foreign body in preauricular region.

Foreign bodies, although they are often found throughout the body, to a lesser degree in the face, still constitute a diagnostic challenge for the trauma surgeon. Its removal means danger of damaging important facial anatomic structures, even if its exact position from the image data was known. So, the objective is to describe a clinical report of a patient (42 years of age, male sex) who experienced falling to the ground, attended by the Department of Surgery and Traumatology Bucco-Maxillo-Facial Surgery, Faculty of Dentistry of Araçatuba, São Paulo State University, and 2 days after the trauma, he reported difficulty in mouth opening and pain. After clinical evaluation, we observed the presence of injury in the left preauricular region already in the process of healing. During the intraoral physical examination, a limitation of the mouth opening was noted. Radiographic posteroanterior and profile of the face showed 2 radiopaque foreign bodies in the left side, lying apparently at the region of the mandibular condylar process. Under local anesthesia, foreign body removal was carried from there with access to it through the preexisting facial injury. Further clinical examinations showed an improvement in mouth opening, absence of pain complaints, and/or functional complaints.

Occurrence of tooth injuries in patients treated in hospital environment in the region of Araçatuba, Brazil during a 6-year period.

Dental trauma has been considered as a public health problem that affects mainly children and youngsters and due to its impact on the patient's quality of life. This study presents the results of a 6-year survey of the occurrence and characteristics of dental trauma in patients admitted to the Service of Surgery and Oral and Maxillofacial Traumatology of the School of Dentistry of Araçatuba (UNESP, Brazil) after emergency care in hospital facilities in the region of Araçatuba, SP, Brazil. For such purpose, the clinical files of patients treated at the Service between 1999 and 2005 were reviewed. Information regarding gender, age, number of traumatized teeth, etiology and diagnosis of the trauma was collected from the files of patients with tooth injuries and recorded in case report forms specifically designed for this purpose. The results showed that from a total of 4112 patients admitted to the Service within the surveyed period, 266 (6.5%) had tooth injuries (172 males - 64.7%; 94 females - 35.3%). The total number of traumatized teeth was 496. Most patients belonged to the 16-20 year-old age group (20.3%) and the most frequent causes of tooth injuries were bicycle accidents (28.6%), motorcycle accidents (19.2%) and falls (18.8%). Injuries to the periodontal tissues were the most frequent type of tooth injuries (408 teeth; 82.26%), occurring in 118 primary and 290 permanent teeth. Among the injuries to the periodontal tissues, avulsion was the most common (32.86%) (29.41% for primary and 34.0% for permanent teeth), followed by extrusive luxation (19.15%) (25.21% for primary and 17.24% for permanent teeth). In conclusion, in the surveyed population, cases of tooth injuries were more frequent in males aged 16-20 years old due to cyclist accidents with predominance of injuries to the periodontal tissues, in particular, avulsions.

Supragingival and subgingival microbiota from patients with poor oral hygiene submitted to radiotherapy for head and neck cancer treatment.

OBJECTIVE: This case-control study aimed to evaluate the effects of conventional radiotherapy (RT) on the prevalence and populations of oral microorganisms in head and neck cancer patients who did not receive adequate preventive dental care. It was hypothesized that side effects of radiotherapy could be associated with radiation dose, microbiological aspects, and socioeconomic conditions of the patients. DESIGN: Twenty-eight dentate patients with head and neck cancer submitted to RT were included in the study. Radiation dose received varied from 4320 to 7020 cGy. Patients with the same demographic and health conditions, but no history of cancer or antineoplastic treatment were used as controls. Clinical examinations were carried out before RT, 15-22 days after starting RT, immediately after and 6 months after RT. Supra and subgingival biofilms were collected and cultivated onto selective and non-selective media. Isolates were identified by biochemical and physiological characteristics. Stimulated and unstimulated salivary flow rate and saliva buffer capacity were also determined. RESULTS: Mucositis, dermatitis, xerostomia, dysgeusia, dysphagia and candidiasis were common after starting RT and during the treatment period. Xerostomia was followed by a decrease in salivary pH and buffer capacity, which showed association with the increase of cariogenic cocci and yeast populations, which were also associated with deterioration of hygiene. Candida and family Enterobacteriaceae showed increased prevalence with RT, and were associated with the occurrence of mucositis and xerostomia. CONCLUSIONS: Modifications in oral biofilms of irradiated patients showed association with xerostomia and hygiene conditions, which reinforces the necessity of improving patient compliance to oral health care programs.

Epidermoid Cyst: Clinical and Surgical Case Report.

The epidermoid cyst is a development cyst, skin, filled with keratin and imprisoned by stratified squamous epithelium similar to skin. They are more frequent in males. Clinically presenting as flabby, prevalent over the face, neck and back. They are usually asymptomatic and its etiology is directly linked to trauma, as well as the imprisonment of epithelial rests during embryonic fusion. This study aims to present a case report of a patient 54 years old, with an epidermal cyst in the face, which was treated surgically.

Enucleation of Odontogenic Cyst with Bone Graft / Enucleación de Quiste Odontogénico con Injerto Óseo

ABSTRACT: Periapical cyst originates from an inflammatory reaction in the body that occurs due to a long-term endodontic aggression. It is more prevalent in caucasian male, during the third decade of life, in the anterior portion of the maxilla. They are commonly radiographic findings, due to their asymptomatic aspect. This study reports a Periapical Cyst in the portion corresponding to teeth 21, 22 and 23, which was treated by enucleation of the cyst, apicoectomy and retrograde root filling with Mineral trioxide aggregate (M.T.A) of teeth 21 and 22, filling of the cyst cavity with xenogeneic bone graft GenOx and a collagen membrane Gen-Derm. Observations after three months show good and rapid bone regeneration, periodontal and periapical health of the teeth involved.

RESUMEN: El quiste periapical se origina de una reacción inflamatoria que ocurre debido a una agresión endodóntica a largo plazo. Es más frecuente en el varón caucásico, durante la tercera década de la vida, en la porción anterior del maxilar. Son comúnmente hallazgos radiográficos, debido a su aspecto asintomático. En este estudio informamos acerca de un quiste periapical, en la porción correspondiente a los dientes 21, 22 y 23, que se trató mediante enucleación, apicectomía y relleno de raíz retrógrada con agregado de trióxido mineral (MTA) de los dientes 21 y 22, relleno de la cavidad del quiste con injerto óseo xenogénico Gen-Ox y una membrana de colágeno GenDerm. Las observaciones después de tres meses mostraron una buena y rápida regeneración ósea, con conservación de la salud periodontal y periapical de los dientes involucrados.

The Use of 2.4-mm Locking Plate System in Treating Comminuted Mandibular Fracture by Firearm.

Maxillofacial trauma caused by firearms has considerably increased, in which the mandibular body is the site of highest incidence of firearm projectiles. In these cases, the use of titanium plates and screws allows the early restoration of form and function of the mandible with stable and predictable results. Recently, conventional plates have been extensively used to treat comminuted mandibular fractures. Nevertheless, the conventional system presents several limitations such as screw compression against the bone interface and the necessity of precise fit of plate to the bone. To overcome such drawbacks, the locking plates have emerged. The present clinical case reported the operative treatment of mandibular fracture caused by firearm projectiles with the use of locking plate. The indications, advantages, and disadvantages of this system are presented.

Osteonecrose dos maxilares associada ao uso de bisfosfonato / Osteonecrosis of the jaws associated with the use of bisphosphonate

Os bisfosfonatos são eficazes na prevenção e tratamento de doenças ósseas onde há uma excessiva atividade osteoclástica, incluindo doença de Paget, Osteoporose, Osteogênese imperfeita, Displasia fibrosa, Mieloma Múltiplo, metástases ósseas de neoplasias de pulmão, mama e próstata. Necrose avascular dos maxilares é cada vez mais reconhecida como uma complicação séria da terapia à longo prazo com bisfosfonatos. O efeito antiangiogênico do bisfosfonato e sua capacidade de causar necrose óssea avascular foram bem reconhecidos. Porém o tratamento ainda se mantém polêmico. Objetivo: o objetivo do presente trabalho foi realizar uma revisão bibliográfica a respeito da osteonecrose associada ao uso de bisfosfonatos, abordando também suas características clínicas e radiográficas, etiopatogenia, tratamento e prevenção. Conclusão: embora alguns protocolos de tratamento tenham sido criados, os mesmo não são consensuais no meio profissional, sendo assim, o foco deve ser a prevenção e divulgação da osteonecrose dos maxilares associada ao uso dos bisfosfonatos..

Introduction: bisphosphonates are effective in the prevention and treatment of bone diseases where there is an excessive osteoclast activity, including Paget`s disease, osteoporosis, osteogenesis imperfecta, fibrous dysplasia, multiple myeloma, bone metastasis from cancers of the lung, breast and prostate. Avascular necrosis of the jaws is increasingly recognized as a serious complication of long-term therapy with bisphosphonates. The antiangiogenic effect of bisphosphonates and their ability to cause avascular bone necrosis were well recognized. However, this treatment still remains controversial. Objective: the objective of this study was to review literature regarding osteonecrosis associated with bisphosphonates also addressing their clinical and radiographic features, pathogenesis, treatment and prevention. Conclusion: despite the use of some protocols to treat osteonecrosis associated with bisphosphonates, one should focus on the prevention and dissemination of information on this complication...

Lipoma intrabucal: um caso atípico / Intraoral lipoma: an atypical case

Lipoma é um tumor benigno de origem mesenquimal composto de tecido adiposo que raramente ocorre na cavidade oral. Apresentam-se com crescimento lento, assintomático, séssil ou pediculado, com prevalência a partir da 4ª década de vida. O prognóstico é favorável, com raras recidivas, e o tratamento de escolha é a excisão cirúrgica. Objetivo: o presente caso relata a presença de um lipoma de língua de tamanho atípico, medindo 3,0 cm x 2,2 cm em suas maiores extensões, que se manifestou entre 06 e 08 meses em uma paciente de 55 anos do gênero feminino. Relato de caso: relatava a paciente que o tumor atrapalhava sua fala e mastigação. A paciente foi submetida à biópsia incisional, sendo diagnosticado através do exame histopatológico o lipoma. O tumor então foi removido completamente sob anestesia local e há 10 meses vem sendo acompanhado sem nenhuma alteração. Conclusão: Devido a inúmeros tumores benignos de tecidos moles, a biópsia incisional é de grande valia para a realização de tratamento de lesões com volumes extensos e de diagnóstico clínico duvidoso...

Lipoma is a benign tumor of mesenchymal origin composed of fatty tissue and rarely occurs in the oral cavity. They are presented with slow growing, asymptomatic, sessile or pediculate, with prevalence from the 4th decade of life. The prognosis is favorable, with rare recurrence, and treatment of choice is surgical excision. Objetive: the present case reports the presence of a tongue lipoma with an atypical size, measuring 3.0 x 2.2 cm in its greatest extension, which was manifested between 06 and 08 months in a 55 years old female patient. Case report: she complained that the tumor interfered with her speech and chewing. The patient underwent incisional biopsy and was diagnosed by histopathologic examination lipoma. Then, tumor was completely removed under local anesthesia and 10 months ago has been followed without any change. Conclusion: due to many types of soft tissue benign tumors the incisional biopsy is essential to the treatment of extensive lesions with doubtful diagnosis...

Radiographic and clinical evaluations of the zygomatico-maxillary complex fractures treated by 2. 0 miniplates system / Evaluaciones radiográficas y clínicas de fracturas del complejo cigomáticomaxilar tratadas por el sistema de mini placas 2. 0

The zygomaticomaxillary complex (ZMC) is the second highly incidental of facial fractures. According to the anatomical complexity, there are many reports in the literature about this trauma, mainly related to treatment for these fractures. With the purpose of evaluating clinically and radiographically the stability of unilateral zygomatic fractures treated by surgical reduction and fixed in two points by stable internal fixation, this research was proposed. Twenty patients with zygomatic fractures were evaluated and compared with twenty nonfractured patients. The results showed that there were no statistically significant differences among the obtained data, perimeter and area, of the treated and contra-lateral sides of the experimental group. When compared to the control group the differences were not statistically significant. We also performed a comparison of the distance between the nasal bone and zygomatic prominence in all groups the results were also satisfactory

Las fracturas del complejo cigomaticomaxilar son las segundas más frecuente del territorio facial. De acuerdo con la complejidad anatómica, existen muchos informes en la literatura sobre este trauma, principalmente relacionadas con el tratamiento de estas fracturas. El propósito de esta investigación fue evaluar clínica y radiográficamente la estabilidad de las fracturas cigomáticas unilaterales tratadas por reducción quirúrgica y fijadas en dos puntos por fijación interna estable. Veinte pacientes con fracturas cigomáticas fueron evaluados y comparados con veinte pacientes sin fracturas. En el grupo experimental, los resultados mostraron que no hubo diferencias estadísticamente significativas entre los datos obtenidos del perímetro y el área de los lados tratados y contralaterales. Al comparar estos datos con el grupo de control las diferencias no fueron estadísticamente significativas. También se realizó una comparación de la distancia entre el hueso nasal y la prominencia cigomática en todos los grupos, estos resultados también fueron satisfactorios

Odontogenic keratocyst tumor: report of two cases / Tumor queratoquiste odontogénico: reporte de dos casos

In 2005, odontogenic cyst was classified as keratocyst odontogenic tumor due to being aggressive and recurrent. The keratocyst odontogenic tumor has characteristics, with slow development, does not cause metastases and provides great bone destruction. The aim of this study was to discuss the aspects regarding the diagnosis, prognosis and treatment of odontogenic keratocyst tumor, through the report of two cases. Both initially underwent decompression of the lesion present proximity of anatomical structures to be great and noble, aiming to prevent pathological fractures. We carried out the clinical-radiographic and after regression of the lesion, patients underwent enucleation total.

El año 2005 quiste odontogénico fue clasificado como un tumor queratoquiste odontogénico (TQO) debido a su agresividad y recurrencia. El TQO tiene las características del tumor: crecimiento lento, no causa metástasis y proporciona una gran destrucción ósea. El objetivo de este estudio fue examinar los aspectos relacionados con el diagnóstico, pronóstico y tratamiento del TQO mediante la presentación de dos casos. Ambos casos fueron inicialmente sometidos a descompresión debido al gran tamaño de la lesión y la proximidad de ésta con estructuras anatómicas importantes, con el objetivo de prevenir las fracturas patológicas. Se llevó a cabo un seguimiento clínico-radiográfico y después de tener una regresión de la lesión postdescompresión, los pacientes fueron sometidos la enucleación total.

Tracionamento ortodôntico-cirúrgico para caninos superiores impactados: revisão crítica e sugestão de protocolo / Surgical-orthodontic traction for impacted maxillary canines: a critical review and suggested protocolc

Maxillary canine impaction is a common occurrence, especially in the palate, despite sufficient space in the arch for tooth alignment. A proper approach requires knowledge of different specialties of dentistry, such as orthodontics, surgery, radiology, and periodontology, which are generally not centered on a single professional. The causes for canine impaction may be either generalized or localized, and the diagnosis should be made through specific clinical and radiographic examination. The prognosis of surgical–orthodontic treatment depends on the position of the canine in relation to the neighboring teeth and height of the alveolar process, in addition to careful surgical technique, considering that there are risks involved, such as ankylosis, loss of tooth vitality, root resorption of the involved tooth and adjacent teeth, and damage to supporting tissues. Given the important role played by impacted maxillary canines, their traction is the treatment of choice in orthodontically treated patients. The present study reviews the literature on important factors to be considered when approaching impacted canines, such as therapeutic possibilities, their advantages and disadvantages.

A impactação de caninos superiores é uma ocorrência freqüente, especialmente na região palatina, ainda que na presença de espaço suficiente para seu alinhamento na arcada. A abordagem exige conhecimentos de diferentes especialidades da odontologia, tais como a ortodontia, cirurgia, radiologia e periodontia e que, em geral, não se centram em um único profissional. Pode ser causada por fatores de ordem geral ou local, sendo que seu diagnóstico deve ser realizado por meio de exames clínico e radiográfico específicos. O prognóstico do tratamento ortodôntico–cirúrgico ira depender da posição do canino em relação aos dentes vizinhos e da sua altura no processo alveolar, além de técnica cirúrgica cuidadosa, haja vista que envolve riscos, como: anquilose, desvitalização, reabsorção radicular do dente envolvido e dos dentes adjacentes e dano aos tecidos de suporte. Em virtude das importantes funções desempenhadas pelos caninos inclusos superiores, o seu aproveitamento através do tracionamento é o tratamento de eleição nos pacientes assistidos ortodonticamente. O presente trabalho traz uma revisão bibliográfica acerca de importantes fatores a serem considerados na abordagem dos caninos impactados, tais como as possibilidades terapêuticas, suas vantagens e desvantagens.

Taxa de sobrevivência de implantes e biomateriais no levantamento do seio maxilar com pneumatização significativa: um estudo retrospectivo com 79 pacientes, durante seis meses a dez anos / Survival rate of immediate dental implants and biomaterials after large sinus lifting procedures: a retrospective study on 79 patients with a 6-month to 10-year follow-up

Objetivo: avaliar retrospectivamente a taxa de sucesso de implantes e biomateriais após o levantamento do seio maxilar. Material e métodos: foram utilizados prontuários de pacientes tratados entre 1998 e 2014, incluídos os que possuíam rebordo maxilar com altura menor do que 5 mm. Todos os procedimentos de enxertia foram realizados com instalação de implante de forma mediata e acesso ao seio maxilar pela janela óssea lateral, colocação do biomaterial, membrana e sutura. Resultados: foram analisados 79 pacientes, tendo sido utilizados dois biomateriais. O tempo de reavaliação variou de seis meses a dez anos. Nos dados de altura óssea radiográfica antes e depois do procedimento (70 implantes, 36 pacientes), houve um aumento médio de altura na região do seio maxilar com o Bio-Oss de 17,23 mm e com o Orthogen de 13,12 mm (p < 0,05), sendo a média geral de 15,17 mm. Na relação sobrevida do implante e enxerto utilizado (92 implantes, 43 pacientes), os valores foram de 96,8% (autógeno) e 98,3% (autógeno + biomaterial). Conclusão: os resultados permitiram concluir que tanto os enxertos autógenos como os biomateriais são alternativas viáveis para os seios maxilares com grande pneumatização.

Objective: to retrospectively evaluate the success rate of implants and biomaterials after maxillary sinus lift. Material and methods: records of patients treated between 1998 and 2014 were used, including those with maxillary ridge height less than 5 mm. All grafting procedures were performed with implant placement and access to the maxillary sinus through the lateral bony window, placement of the biomaterial, membrane and suture. Results: 79 patients were analyzed and two biomaterials were used. The follow-up period ranged from six months to ten years. In the radiographic bone height data before and after the procedure (70 implants, 36 patients), there was a mean increase in height in the maxillary sinus region with the Bio-Oss of 17.23 mm and with the Orthogen of 13.12 mm (p < 0.05), the overall mean being 15.17 mm. Regarding implant and graft survival (92 implants, 43 patients), the values were 96.8% (autogenous) and 98.3% (autogenous + biomaterial). Conclusion: both autogenous grafts and biomaterials are viable alternatives for the maxillary sinuses with great pneumatization.

Tratamento conservador de fratura de arco zigomático: uma visão conservadora / Conservative treatment of fracture of the zygomatic arch: a conservative view

Introdução: O complexo zigomático maxilar é a segunda área da face mais atingida por injúrias, superada, apenas, pelos ossos nasais. O arco zigomático fratura-se sob ação direta de traumas (soco, cabeçada), graças à sua estrutura frágil, perdendo a curvatura convexa normal na área temporal. Objetivo: Relatar um caso clínico, utilizando uma técnica de redução de fratura do arco zigomático por meio de um gancho de Ginestet ou de Barros. Relato do caso: Paciente do sexo masculino, 25 anos de idade, leucoderma, apresentando fratura do arco zigomático a direita. O tratamento realizado foi redução incruenta com o gancho adaptado sobre a pele. O paciente foi instruído a manter cuidado com a região por pelo menos quatro semanas.

Introduction: The zygomatic maxillary complex is the second most affected area of the face due to injuries, surpassed only by the nasal bones. The zygomatic arch fracture is under the direct action of trauma (punch, head butt), its fragile structure, losing its normal convex curvature in the temporal area. Objective: To report a case, using a reduction technique of zygomatic arch fracture using a hook or Ginestet Barros. Case report: A male patient, 25 years of age, leukoderma, showing zygomatic arch fracture right. The patient underwent closed reduction with the hook adapted to the skin. The patient was instructed to keep care of the region for at least four weeks.

Contaminação Microbiana das Soluções de Processamento Radiográfico: Risco de Infecção Cruzada / Microbial Contamination of Radiographic Developing and Fixing Solutions: Risk of Cross Infection

Objetivo: Avaliar a contaminação microbiana em amostras de revelador e fixador radiográficos água de processamento radiográfico, água do equipo odontológico e da fonte externa de água do consultório. Método: Amostras de 50 consultórios particulares e de 20 consultórios da Faculdade de Odontologia de Araçatuba-UNESP foram coletadas. As amostras foram submetidas à neutralização dos agentes inibitórios residuais e submetidas à pré-enriquecimento em água peptonada e caldo EVA e inoculadas em meios de cultura seletivos e não seletivos. O DNA nas amostras foi extraído e a presença de microrganismos superinfectantes foi avaliada por PCR. As diferenças na prevalência dos microrganismos nas amostras de soluções foram submetidas ao teste análise de variância de medidas repetitivas para dados categóricos, enquanto os testes de Qui-Quadrado e de Mann-Whitney foram utilizados para avaliar a existência de possíveis associações entre o número total de microrganismos heterotróficos e os protocolos de controle de infecção. O nível de significância adotado foi de 5%.Resultados: Verificou-se que as amostras de fixador eram contaminadas em uma freqüência muito menor do que o revelador e água de processamento radiográfico, sendo que essa última mostrava microrganismos tipicamente originados da microbiota bucal e da pele humanas, bem como microrganismos freqüentemente encontrados na água do equipo odontológico. A contaminação da água de processamento foi menor em consultórios onde o invólucro da película radiográfica recebia desinfecção antes do processamento ou era recoberto com filme plástico. Adicionalmente, todas as amostras de água da fonte externa do consultório se mostraram potáveis.Conclusão: A utilização de barreiras filmes plásticos de proteção a película mostrou ser a melhor forma de reduzir a contaminação dessas soluções

Objective: To evaluate the microbial contamination in samples of radiographic developing and fixing solutions, radiographic processing water, dental unit waterlines and dental officeÆs external water supply. Methods: Samples of 50 private dental offices and 20 dental offices of the School of Dentistry de Araçatuba-UNESP were collected. The samples were subjected to neutralization of the residual inhibitory agents followed by pre-enrichment in peptone water and EVA broth and were inoculated in selective and non-selective culture media. The DNA of the samples was extracted and the presence of superinfectant microorganisms was evaluated by PCR. The differences in the prevalence of the microorganisms in the samples of the solutions were analyzed by repeated-measures analysis of variance for category data, while the Chi-Square and Mann-Whitney tests were used to evaluate the existence of associations between the total number of heterotrophic microorganisms and infection control protocols. The significance level was set at 5%.Results: Samples of radiographic fixing solution presented a remarkably less frequent contamination than radiographic developing solution and radiographic processing water. The latter exhibited microorganisms typically originated from the oral microbiota and human skin as well as microorganisms frequently found in dental unit waterlines. There was less contamination of radiographic processing water in the dental offices where the x-ray film packets were disinfected before processing or were covered by a plastic film. Additionally, all samples of dental officeÆs external water supply were drinkable.Conclusion: Covering the x-ray film packets with a plastic film protection barrier was proven the best manner to reduce the contamination of the evaluated solutions