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1.
J Infect Dis ; 216(8): 936-944, 2017 11 15.
Artigo em Inglês | MEDLINE | ID: mdl-29149338

RESUMO

Background: Respiratory tract infections are frequent causes of hospitalization and initiation of empirical antimicrobial therapy. Testing for a broad panel of respiratory viruses has been advocated as a useful tool for antibiotic stewardship. We conducted a prospective observational study to assess the impact of rapid viral test results on antimicrobial prescriptions and clinical outcomes among hospitalized adults. Methods: Eight hundred patients admitted with respiratory symptoms were tested by a 12-virus respiratory panel (RVP) during 3 consecutive winters in Montreal, Canada. The primary outcome measure was change in antimicrobial prescriptions (ie, de-escalation of empirical antimicrobial therapy or commencement of new antimicrobial therapy) after RVP results were available. Clinical outcomes were also assessed. Results: Influenza virus was identified in 53% of individuals in the study population, and other viruses were identified in 10%. Influenza virus positivity was associated with shorter duration of hospitalization and appropriate antiviral management. Antibiotic management was most significantly correlated with radiographic suspicion of pneumonia and less so with results of the RVP. Positivity for viruses other than influenza virus was not correlated with significantly different outcomes. Conclusions: Physicians respond to results of testing for influenza virus when managing hospitalized adult patients but respond less to test results for other viruses. These data can inform the design of stewardship interventions and the selection of viral testing panels for hospitalized patients.


Assuntos
Anti-Infecciosos/uso terapêutico , Gestão de Antimicrobianos , Infecções Respiratórias/diagnóstico , Viroses/diagnóstico , Vírus/isolamento & purificação , Idoso , Idoso de 80 Anos ou mais , Antivirais/uso terapêutico , Canadá , Feminino , Hospitalização , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase Multiplex , Nasofaringe/virologia , Estudos Prospectivos , Infecções Respiratórias/tratamento farmacológico , Infecções Respiratórias/virologia , Viroses/tratamento farmacológico , Viroses/virologia , Vírus/genética
2.
Methods Mol Biol ; 801: 41-63, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-21987246

RESUMO

There are many methods presently available to produce recombinant proteins in mammalian systems. The BacMam system is a simple straightforward method which overlaps two well-established technologies, namely the BEVS insect cell system and the transduction of mammalian cells in vitro. This chapter describes a method for the study of gene expression in mammalian cells in a series of simple steps. Protocols outlined include the design and construction of the recombinant baculovirus, cell culture techniques required to maintain both insect and mammalian cells, generation of baculovirus stocks, and methods to obtain maximal and reproducible gene expression in mammalian cells. Currently available statistical techniques using factorial design of experiment to optimize conditions for recombinant protein in vitro are outlined. Then details with respect to process scale-up in disposable bioreactors are included.


Assuntos
Baculoviridae/genética , Engenharia Genética/métodos , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Vírion/genética , Animais , Reatores Biológicos , Linhagem Celular , Expressão Gênica , Vetores Genéticos/genética , Células HEK293 , Humanos , Insetos/citologia , Microscopia , Proteínas Recombinantes/biossíntese , Transdução Genética
3.
Biotechnol Bioeng ; 97(2): 332-45, 2007 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-17054119

RESUMO

The development of insect cells expressing recombinant proteins in a stable continuous manner is an attractive alternative to the BEV system for recombinant protein production. High cell density fed batch and continuous perfusion processes can be designed to maximize the productivity of stably transformed cells. A cell line (Sf-9SEAP) expressing high levels of the reporter protein SEAP stably was obtained by lipid-mediated transfection of Sf-9 insect cells and further selection and screening. The expression of the Sf-9SEAP cells was compared with the BEVS system. It was observed that, the yield obtained in BEVS was similar to the batch Sf-9SEAP at 8 and 7 IU/mL, respectively. The productivity of this foreign gene product with the stable cells was enhanced by bioprocess intensification employing the fed-batch and perfusion modes of culture to increase the cell density in culture. The fed batch process yielded a maximum cell density of 28 x 10(6) cells/mL and 12 IU/mL of SEAP. Further improvements in the productivity could be made using the perfusion process, which demonstrated a stable production rate for extended periods of time. The process was maintained for 43 days, with a steady-state cell density of 17-20 x 10(6) cells/mL and 7 IU/mL SEAP. The total yield obtained in the perfusion process (394 IU) was approximately 22 and 8 times higher than that obtained in a batch (17.6 IU) and fed batch (46.1 IU) process, respectively.


Assuntos
Fosfatase Alcalina/biossíntese , Biotecnologia/métodos , Proliferação de Células , Fosfatase Alcalina/metabolismo , Animais , Baculoviridae/genética , Reatores Biológicos , Técnicas de Cultura de Células/métodos , Células Cultivadas , Perfusão , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Spodoptera
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