Dehydrated citrus pulp alters feedlot performance of crossbred heifers during the receiving period and modulates serum metabolite concentrations before and after an endotoxin challenge.

English × Continental heifers ( = 180) were sourced in 2 loads (219.3 ± 16.0 and 221.4 ± 16.4 kg, respectively) from commercial auction barns to study the effects of feeding dehydrated citrus pulp (DCP) on feedlot performance of newly received heifers. A completely randomized block design was used with BW nested within arrival load and blocked by BW into 3 dietary treatments (36 pens, 5 heifers/pen, 12 blocks, 3 pens/block, and 12 pens/treatment). Treatment diets contained 1) 0% DCP (control diet [CON]), 2) 10% DCP, or 3) 20% DCP on a DM basis. Diets containing DCP were exchanged with steam-flaked corn on a 1:1 basis. Cattle were fed a 63, 73, and 83% concentrate diet from d 0 to 28, d 28 to 42, and d 42 to 56, respectively. Over the 56-d trial period, as the amount of dietary DCP increased, DMI decreased ( = 0.01), ADG decreased ( < 0.01), and G:F decreased ( = 0.02). From d 0 to 28, there was no difference in the observed minus the predicted NEg of the diet ( = 0.73); from d 28 to 42, there was a linear increase in NEg favoring DCP treatments ( < 0.01); and from d 42 to 56, there was a linear decrease in NEg against the DCP treatments ( < 0.01). At the conclusion of the trial, a subset of heifers ( = 22; 307.89 ± 3.32 kg on d 63) were used to evaluate blood metabolite concentrations before and after a lipopolysaccharide (LPS) challenge. On d 63, heifers were fitted with jugular catheters and moved into individual stalls. On d 64, heifers were intravenously challenged with LPS (0.5 µg/kg BW), and blood samples were collected every 0.5 h from -2 to 8 h and at 24 h relative to the LPS challenge (0 h). Serum glucose, serum urea nitrogen (SUN), and NEFA concentrations were determined. Cattle lost less weight at both 24 and 72 h after the LPS challenge with increasing DCP percentage ( < 0.01). Glucose ( = 0.12) and NEFA ( = 0.13) concentrations did not differ before the LPS challenge; however, there was a treatment effect for SUN, with elevated concentrations of SUN in CON cattle ( < 0.01). After the LPS challenge, DCP-fed cattle had reduced glucose, elevated NEFA, and reduced SUN concentrations ( ≤ 0.01). Results indicate that dietary DCP modulated metabolite concentrations in heifers following an endotoxin challenge and affected feedlot performance when incorporated in receiving diets in replacement of corn. Future studies will need to address strategies to increase DMI or explore levels of DCP less than 10% in the diet of newly received heifer calves.

BAY u9773, a novel antagonist of cysteinyl-leukotrienes with activity against two receptor subtypes.

The effects of BAY u9773 (6(R)-(4'-carboxyphenylthio)-5(S)-hydroxy-7(E),9(E), 11(Z),14(Z)-eicosatetraenoic acid), a cysteinyl-leukotriene analogue, were investigated on a variety of smooth muscle preparations in order to determine its profile as a cysteinyl-leukotriene receptor antagonist. The tissues were contracted with leukotriene C4 or leukotriene D4 and their receptor characteristics defined as either 'typical' or 'atypical' according to the activity or inactivity, respectively, of the selective antagonists ICI 198615, MK 571 and SKF 104353. BAY u9773 antagonised 'typical' cysteinyl-leukotriene receptors with pA2 (or pKB) values in the range 6.8-7.4 and also antagonised 'atypical' receptors with pA2 values in the range 6.8-7.7. However, BAY u9773 had no effect at 10(-6) M against a selection of non-leukotriene stimuli in the same preparations. BAY u9773 competitively displaced [3H]leukotriene D4 binding to guinea-pig lung homogenate, with a pKi of 7.0 +/- 0.1. In the guinea-pig lung strip, BAY u9773 was found to be inactive at 10(-6)M against leukotriene C4- and leukotriene D4-induced contractions, which may suggest the existence of a third type of cysteinyl-leukotriene receptor. These data demonstrate that BAY u9773 is a selective cysteinyl-leukotriene receptor antagonist with comparable activity at both 'typical' and 'atypical' receptors and as such represents a valuable tool for the study of cysteinyl-leukotriene receptors.

Chromium supplementation alters both glucose and lipid metabolism in feedlot cattle during the receiving period.

Crossbred steers (n = 20; 235 ± 4 kg) were fed for 53 d during a receiving period to determine if supplementing chromium (Cr; KemTRACE Chromium Propionate 0.04%, Kemin Industries, Des Moines, IA) would alter glucose or lipid metabolism of newly received cattle. Chromium premixes were supplemented to add 0 (Con) or 0.2 mg/kg of Cr to the total diet on a DM basis. Cattle were fitted with jugular catheters on d 52. A glucose tolerance test (GTT) and an insulin sensitivity test (IST) were conducted on d 53. Blood samples were collected from -60 to 150 min relative to each infusion. Serum was isolated to determine glucose, insulin, and NEFA concentrations. Throughout GTT, no differences were detected in glucose concentrations, glucose clearance rates (k), or preinfusion insulin concentrations (P > 0.50), but insulin concentrations postinfusion tended (P = 0.06) to be greater for the Cr-supplemented steers. This caused an increase in the insulin to glucose ratio (I:G) from 0 to 150 min postinfusion for the Cr-supplemented steers (P = 0.03). In addition, NEFA concentrations during GTT were lower (P ≤ 0.01) for Cr-supplemented steers both preinfusion and postinfusion. During IST, there was no treatment effect on glucose concentrations preinfusion (P = 0.38), but postinfusion glucose concentrations were greater (P< 0.01) in the Cr-supplemented steers. The k of Cr-supplemented steers tended (P = 0.06) to be faster than Con steers from 30 to 45 min postinfusion. During the same test, there was no treatment effect detected for insulin concentrations (P > 0.33). The I:G were not affected by treatment (P > 0.40).Concentrations of NEFA were reduced (P < 0.01) both preinfusion and postinfusion during IST for Cr-supplemented steers. Results of this study indicate that supplementation of Cr can significantly alter lipid metabolism. This suggests that these steers had less dependence on lipid metabolism for energy or sensitivity of adipose tissue to antilipolytic signals was reduced. Results of glucose and insulin metabolism were inconsistently modified after a GTT and an IST.

Chromium supplementation alters the performance and health of feedlot cattle during the receiving period and enhances their metabolic response to a lipopolysaccharide challenge.

Crossbred steers (n = 180; 230 ± 6 kg) were fed during a 56-d receiving period to determine if supplementing chromium (Cr; KemTRACE Chromium Propionate 0.04%, Kemin Industries) would improve feedlot performance and health of newly-received cattle. A completely randomized block design (36 pens; 9 pens/treatment; 5 steers/pen) was used. Chromium premixes were supplemented to add 0 (Con), 0.1, 0.2, or 0.3 mg/kg of Cr to the total diet on a DM basis. No differences were detected on d 0 or after the first 14 d on feed. From d 0 to d 28, DMI (P = 0.07) and ADG increased linearly (P = 0.04) as Cr concentrations increased. From d 0 to d 56, BW (P = 0.08) displayed a tendency to increase linearly, and consequently ADG and G:F increased linearly (P ≤ 0.05) as Cr concentrations increased. The number of steers treated at least once for respiratory symptoms tended (P = 0.07) to linearly decrease as Cr concentrations increased. Twenty additional steers (235 ± 4 kg) were fed 56 d to determine if supplementing Cr (Con or 0.2 mg/kg Cr) would alter the metabolic response of newly-received cattle to a lipopolysaccharide (LPS) challenge. Cattle were fitted with jugular catheters on d 52. On d 55, blood samples were collected at 0.5-h intervals from -2 to 8 h, and again at 24 h relative to a LPS challenge (0.5 µg/kg BW) at 0 h. Serum glucose, insulin, and NEFA concentrations were determined from blood samples. Steer BW was also measured at cannulation, and 24 h and 8 d post-LPS. Steer BW did not differ at cannulation (P = 0.37), but 24 h post-LPS, Cr-supplemented steers had lost less BW (P = 0.03). Pre-LPS glucose concentration did not differ (P = 0.97). Post-LPS, there was a time × treatment interaction (P < 0.01) such that glucose concentration peaked earlier (0.5 h) and at a greater concentrations in Cr-supplemented steers (P < 0.01). Insulin concentration did not differ between treatments pre- or post-LPS (P > 0.13). Concentration of NEFA did not differ pre-LPS (P = 0.54); but 0.5 h post-LPS Cr-treated steers produced a greater peak NEFA concentration (P < 0.04). Results of this study indicate that supplementation of Cr to the basal diet can have beneficial effects on the performance and health of newly-received steers. These data also suggest that supplementation of Cr attenuated BW loss and allowed for a quicker recovery after a LPS challenge.

Early citizen review: does it make a difference?

Case review, designed to promote adequate and appropriate planning and service delivery for children in foster care, is expected to reduce time in foster care and to lead to permanent placement outcomes. This article reports the results of a study on the impact of external citizen review initiated 14 to 45 days after a child entered the court system. Comparisons were made between two study groups to determine the impact of early citizen review on services planned and provided, court delays, completeness of the written case plans, number of placements, time in placement, and achievement of permanent placement. Implications for policy and practice are discussed.

A study of temperature changes occurring in setting dental cements.

A study has been made of the rise in temperature associated with the setting of dental cements. Results are discussed in terms of the chemistry of the cement systems and are related to specification testing and clinical usage. Zinc oxide cements evolve more heat than those based on ion-leachable glasses, and phosphate bonded cements evolve more heat than polycarboxylate cements.

Assessing the accuracy of an automated coding system in emergency medicine.

Accuracy and speed are imperative when it comes to coding medical records. Completely automated approaches to coding are faster than human coders, but are they as accurate? To measure accuracy, a "gold standard" is required; however, establishing a standard for medical records coding is problematic given the inherent ambiguity in some of the coding rules and guidelines. This paper presents statistics regarding the variability amongst experienced coders and compares this variability with an automated system, LifeCode. The authors conclude that LifeCode is as accurate as the human coders used in this study and offers the potential for increased coding consistency and productivity.