RESUMO
BACKGROUND: An avian papillomavirus genome has been cloned from a cutaneous exophytic papilloma from an African grey parrot (Psittacus erithacus). The nucleotide sequence, genome organization, and phylogenetic position of the Psittacus erithacus papillomavirus (PePV) were determined. This PePV sequence represents the first complete avian papillomavirus genome defined. RESULTS: The PePV genome (7304 basepairs) differs from other papillomaviruses, in that it has a unique organization of the early protein region lacking classical E6 and E7 open reading frames. Phylogenetic comparison of the PePV sequence with partial E1 and L1 sequences of the chaffinch (Fringilla coelebs) papillomavirus (FPV) reveals that these two avian papillomaviruses form a monophyletic cluster with a common branch that originates near the unresolved center of the papillomavirus evolutionary tree. CONCLUSIONS: The PePV genome has a unique layout of the early protein region which represents a novel prototypic genomic organization for avian papillomaviruses. The close relationship between PePV and FPV, and between their Psittaciformes and Passeriformes hosts, supports the hypothesis that papillomaviruses have co-evolved and speciated together with their host species throughout evolution.
Assuntos
Genoma Viral , Proteínas Imediatamente Precoces/genética , Papillomaviridae/genética , Papagaios/virologia , Regiões 5' não Traduzidas/genética , Animais , Clonagem Molecular , DNA Viral/genética , Genes Virais/genética , Dados de Sequência Molecular , Fases de Leitura Aberta/genética , Infecções por Papillomavirus/genética , Filogenia , Sequências Reguladoras de Ácido Nucleico/genética , Análise de Sequência de DNA , Aves Canoras/virologia , Infecções Tumorais por Vírus/genética , Proteínas Estruturais Virais/genéticaAssuntos
Academias e Institutos , Neoplasias Pulmonares , Academias e Institutos/história , História do Século XX , História do Século XXI , Humanos , Kentucky/epidemiologia , Neoplasias Pulmonares/etiologia , Neoplasias Pulmonares/mortalidade , Neoplasias Pulmonares/terapia , Fumar/efeitos adversos , Universidades/históriaRESUMO
A novel canine papillomavirus, CfPV-2, was cloned from a footpad lesion of a golden retriever. Unlike the known canine oral papillomavirus (COPV), which has a double-stranded DNA genome size of 8607 bps, the genome of CfPV-2 is 8101 bps. Some of this size difference is due to an abbreviated early-late region (ELR), which is 1200 bps shorter than that of COPV. However, CfPV-2 has other differences from COPV, including the presence of an E5 ORF between the E2 gene and the ELR and an enlarged E4 ORF (one of the largest PV E4 open reading frames). The genome of CfPV-2 shares low homology with all the other papillomaviruses and, even in the most highly conserved ORF of L1, the nucleotide sequence shares only 57% homology with COPV. Due to this highly divergent DNA sequence, CfPV-2 establishes a new PV genus, with its closest phylogenetic relatives being amongst the Xi and Gamma genuses. CfPV-2 also has unique biological features; it induces papillomas on footpads and interdigital regions which, if infection is persistent, can progress to highly metastatic squamous cell carcinoma. CfPV-2 does not induce oral papillomas in immunocompetent animals and antibodies generated against COPV and CfPV-2 are type-specific. The availability of a new canine papillomavirus with differing genetic and biological properties now makes it possible to study type-specific host immune responses, tissue tropism and the comparative analysis of viral gene functions in the dog.
Assuntos
Doenças do Cão/virologia , Papillomaviridae/classificação , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/veterinária , Animais , Carcinoma de Células Escamosas/veterinária , Carcinoma de Células Escamosas/virologia , DNA Viral/química , DNA Viral/genética , Doenças do Cão/patologia , Cães , Pé/patologia , Pé/virologia , Genoma Viral , Histocitoquímica , Lambdapapillomavirus/genética , Microscopia Eletrônica de Transmissão , Dados de Sequência Molecular , Fases de Leitura Aberta , Papiloma/veterinária , Papiloma/virologia , Papillomaviridae/ultraestrutura , Infecções por Papillomavirus/patologia , Infecções por Papillomavirus/virologia , Filogenia , Análise de Sequência de DNA , Deleção de Sequência , Homologia de Sequência do Ácido Nucleico , Vírion/ultraestruturaRESUMO
Utilizando la técnica de hibridización in situ, se investigó la presencia de papilomavirus humano (PVH) en el material biópsico proveniente de 10 pacientes con cáncer del cuello uterino con recurrencia pelviana luego de radioterapia. Se utilizaron sondas de ácido ribonucleico (ARN) marcado con el radioisótopo S35 para los tipos de PVH 6, 11, 16 y 18. Nueve de estas pacientes tenían también biópsias del tumor primario antes de la radioterapia. Se detectó el PVH 16 en el tumor primario y recurrente en cuatro pacientes, y solamente en el primario en un caso. También se encontró PVH 16 en un cáncer recurrente que no tenía biópsias evaluables del tumor primario. La comparación del tumor primario y recurrente evidenció que la radioterapia no altera la señal de hibridización. La persistencia del PVH 16 en el cáncer recurrente del cérvix dá fundamento a la teoría que el PVH jugaría un rol en el mantenimiento del estado maligno