RESUMO
A nanoscale biodegradable polymersome with pH-tuning on-off membrane is prepared via the self-assembly of poly(ß-amino ester)-based amphiphilic copolymers. The pH-sensitive polymersome-like vesicle structure includes two layers that can encapsulate either hydrophobic or hydrophilic therapeutic drugs at physiological pH 7.4. Below a pH of 7.0, the polymersome membrane forms tunnels through which the drug cargo can be rapidly released. The size and morphology of the polymersome are measured by transmission electron microscopy (TEM) and dynamic light scattering (DLS). The pH sensitivity is confirmed by fluorescence spectroscopy. The pH-sensitive drug-delivery polymersome provides a simple and powerful smart carrier for the delivery and controlled release of drugs.
Assuntos
Sistemas de Liberação de Medicamentos , Membranas/ultraestrutura , Polímeros/química , Humanos , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Células MCF-7 , Espectroscopia de Ressonância Magnética , Membranas/química , Microscopia Eletrônica de TransmissãoRESUMO
Physical polymeric hydrogels have significant potential for use as injectable depot drug/protein-delivery systems. In this study, a series of novel injectable, biodegradable and pH/temperature-sensitive multiblock co-polymer physical hydrogels composed of poly(ethylene glycol) (PEG) and poly(ß-amino ester urethane) (PEU) was synthesized by the polyaddition between the isocyanate groups of 1,6-diisocyanato hexamethylene and the hydroxyl groups of PEG and a synthesized monomer BTB (or ETE) in chloroform in the presence of dibutyltin dilaurate as a catalyst. The synthesized co-polymers were characterized by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy and gel-permeation chromatography. Aqueous solutions of the co-polymers showed a sol-to-gel phase transition with increasing pH and a gel-to-sol phase transition with increasing temperature. The gel regions covered the physiological conditions (37°C, pH 7.4) and could be controlled by changing the molecular weight of PEG, PEG/PEU ratio and co-polymer solution concentration. A gel formed rapidly in situ after injecting the co-polymer solution subcutaneously into SD rats and remained for more than 2 weeks in the body. The cytotoxicity tests confirmed the non-cytotoxicity of this co-polymer hydrogel. The controlled in vitro release of the model anticancer drug, doxorubicin, from this hydrogel occurred over a 7-day period. This hydrogel is a potential candidate for biomedical applications and drug/protein-delivery systems.