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As an essential enzyme in the uridine diphosphate (UDP)-GlcNAc biosynthesis pathway, the significant role of N-acetylglucosamine phosphate mutase (AGM) remains unknown in plants. In the present study, a functional plant AGM (AtAGM) was identified from Arabidopsis thaliana. AtAGM catalyzes the isomerization of GlcNAc-1-P and GlcNAc-6-P, and has broad catalytic activity on different phosphohexoses. UDP-GlcNAc contents were significantly decreased in AtAGM T-DNA insertional mutants, which caused temperature-dependent growth defects in seedlings and vigorous growth in adult plants. Further analysis revealed that protein O-GlcNAcylation but not N-glycosylation was dramatically impaired in Atagm mutants due to UDP-GlcNAc shortage. Combined with the results from O-GlcNAcylation or N-glycosylation deficient mutants, and O-GlcNAcase inhibitor all suggested that protein O-GlcNAcylation impairment mainly leads to the phenotypic variations of Atagm plants. In conclusion, based on the essential role in UDP-GlcNAc biosynthesis, AtAGM is important for plant growth mainly via protein O-GlcNAcylation-level regulation.
Assuntos
Arabidopsis , Arabidopsis/metabolismo , Acetilglucosamina/metabolismo , Proteínas/metabolismo , Glicosilação , Fosfatos/metabolismo , Processamento de Proteína Pós-TraducionalRESUMO
Chitosan oligosaccharide (COS) is an effective plant immunity elicitor; however, its induction mechanism in plants is complex and needs further investigation. In this study, the Arabidopsis-Pseudomonas syringae pv. tomato DC3000 (hereafter called DC3000) interaction was used to investigate the induction effect and the underlying mechanisms of COS. COS is effective in inducing resistance to DC3000 in Arabidopsis, and our results demonstrate that treatment with COS 3 days before DC3000 inoculation provided the most effective resistance. Disease severity in jar1 (jasmonic acid [JA]-deficient mutant), NahG, and sid2 (salicylic acid [SA]-deficient mutants) suggest both the SA and JA pathways are required for the Arabidopsis response to DC3000. COS pretreatment induced resistance in wild type (WT), jar1, and also, although to a lesser degree, in NahG and sid2 plants, implying that the SA and JA pathways play redundant roles in COS-induced resistance to DC3000. In COS-pretreated plants, expression of genes related to the SA pathway (PR1, PR2, and PR5) and SA content increased in both WT and jar1. Moreover, expression of genes related to the JA pathway (PDF1.2 and VSP2) and JA content both increased in WT and NahG. In conclusion, COS induces resistance to DC3000 in Arabidopsis by activating both SA- and JA-mediated pathways, although SA and JA pathways play redundant roles in this COS-induced resistance.
Assuntos
Arabidopsis/imunologia , Quitosana/farmacologia , Oligossacarídeos/farmacologia , Doenças das Plantas/imunologia , Imunidade Vegetal/efeitos dos fármacos , Pseudomonas syringae/fisiologia , Arabidopsis/efeitos dos fármacos , Arabidopsis/microbiologia , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Ciclopentanos/metabolismo , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Transferases Intramoleculares/genética , Transferases Intramoleculares/metabolismo , Oxigenases de Função Mista/genética , Oxigenases de Função Mista/metabolismo , Mutação , Nucleotidiltransferases/genética , Nucleotidiltransferases/metabolismo , Oxilipinas/metabolismo , Doenças das Plantas/microbiologia , Reguladores de Crescimento de Plantas/metabolismo , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/imunologia , Folhas de Planta/microbiologia , Ácido Salicílico/metabolismo , Transdução de Sinais/efeitos dos fármacosRESUMO
Chitosan oligosaccharide (COS), derived through hydrolysis of chitosan, has been proved to be an effective plant immunity elicitor, eco-friendly, and easily soluble in water, and influenced several secondary metabolites content to improve fruit qualities. COS are widely used in agriculture to improve the defense response in plants. The purpose of this study was to investigate the pre-harvest treatment effect of COS on the quality of strawberry (Fragaria × ananassa cv.qingxiang). COS was dissolved in distilled water at a concentration of 50 mg·L-1 and sprayed at four different growth stages of strawberry plants, namely seedling stage, before flowering, fruit coloring (the stage of fruit from white to red) and full bloom. Uniform size, shape, color, without any visible damage, and disease-free fruits were harvested for determining the quality. The results showed that the fruit firmness, viscosity, lignin, sugars, protein, total soluble solid, and titratable acidity content increased in COS-treated fruits compared to control. In addition, COS pre-harvest treatment had a positive effect on anthocyanin, total phenol, flavonoid, vitamin C content and DPPH(2,2-diphenyl-1-picrylhydrazyl) scavenging activity of strawberry. Moreover, COS also increased the cell wall composition and regulated gene expression of some important enzymes involved in ethylene compound biosynthesis and cell wall degradation. The finding of this study suggests that pre-harvest application of COS is very useful for improving quality and antioxidant capacity of strawberry.
Assuntos
Quitosana/farmacologia , Produtos Agrícolas/efeitos dos fármacos , Fragaria/efeitos dos fármacos , Fragaria/metabolismo , Oligossacarídeos/farmacologia , Antocianinas/metabolismo , Ácido Ascórbico/metabolismo , Compostos de Bifenilo/metabolismo , Parede Celular/química , Parede Celular/enzimologia , Parede Celular/metabolismo , Quitosana/química , Produtos Agrícolas/química , Produtos Agrícolas/metabolismo , Flavonoides/metabolismo , Flores/química , Flores/metabolismo , Qualidade dos Alimentos , Fragaria/química , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Oligossacarídeos/química , Picratos/metabolismo , Plântula/química , Plântula/metabolismo , Água/químicaRESUMO
Rhizoctonia solani (R. solani) is an important pathogenic fungus that causes symptoms of sheath blight, and the polysaccharide-rich cell wall plays a major role in plant-pathogen interactions. However, the composition and structure of its cell wall polysaccharides are insufficiently understood, and its specific function in plant-pathogen interactions is unknown, which makes effective control of sheath blight difficult at present. Herein, five cell wall polysaccharides (WF-1, WF-2, CAF-1, HAF-1 and HAF 2-1) were sequentially extracted by boiling water, cold and hot alkali from R. solani AG1 IA. They were heteropolysaccharides containing mainly glucose, mannose and galactose and less fucose, with molecular weights above 1100 kDa. These five polysaccharides mainly composed of â4)-Glcp-(1â, â6)-Glcp-(1â, â4,6)-Glcp-(1â, â3,4)-Glcp-(1â, and Manp-(1â. Several polysaccharides, except WF-1, showed different induced resistance degrees on rice plant, with HAF 2-1 having the most significant effect. Further analysis using NMR confirmed that the backbone of HAF 2-1 mainly consisted of â4)-α-D-Glcp-(1â and â6)-α-D-Glcp-(1â with branches of â4,6)-D-Glcp-(1â. HAF 2-1 enhance the resistance of rice against R. solani through salicylic acid (SA)-mediated immune signaling pathway. This work improves our knowledge of the cell wall polysaccharides in plant pathogens and facilitates the study of pathogenic mechanisms and effective disease control.
Assuntos
Oryza , Doenças das Plantas , Doenças das Plantas/microbiologia , Rhizoctonia , Parede Celular , Oryza/microbiologia , PolissacarídeosRESUMO
Phytopathogen cell wall polysaccharides have important physiological functions. In this study, we isolated and characterized the alkali-insoluble residue on the inner layers of the Rhizoctonia solani AG1 IA cell wall (RsCW-AIR). Through chemical composition and structural analysis, RsCW-AIR was mainly identified as a complex of chitin/chitosan and glucan (ChCsGC), with glucose and glucosamine were present in a molar ratio of 2.7:1.0. The predominant glycosidic bond linkage of glucan in ChCsGC was ß-1,3-linked Glcp, both the α and ß-polymorphic forms of chitin were presented in it by IR, XRD, and solid-state NMR, and the ChCsGC exhibited a degree of deacetylation measuring 67.08 %. RsCW-AIR pretreatment effectively reduced the incidence of rice sheath blight, and its induced resistance activity in rice was evaluated, such as inducing a reactive oxygen species (ROS) burst, leading to the accumulation of salicylic acid (SA) and the up-regulation of SA-related gene expression. The recognition of RsCW-AIR in rice is partially dependent on CERK1.
Assuntos
Parede Celular , Quitina , Quitosana , Glucanos , Oryza , Doenças das Plantas , Rhizoctonia , Rhizoctonia/efeitos dos fármacos , Oryza/microbiologia , Oryza/química , Parede Celular/química , Quitosana/química , Quitosana/farmacologia , Quitina/química , Quitina/farmacologia , Glucanos/química , Glucanos/farmacologia , Doenças das Plantas/microbiologia , Resistência à Doença , Espécies Reativas de Oxigênio/metabolismoRESUMO
Steviol glycosides (SGs) are a variety of important natural sweeteners. They are 200-350 times sweeter than sucrose without calories. Currently, their production is still mainly dependent on extraction from Stevia rebaudiana Bertoni (stevia). Oligosaccharides are environmentally friendly elicitors that promote plant growth and accumulation of secondary metabolites. In the present study, different concentrations of chitosan oligosaccharides (COS) and alginate oligosaccharides (AOS) were applied to stevia to explore their effect on growth and SGs biosynthesis. It was found that both COS and AOS promoted biomass production by increasing the leaf number and photosynthetic efficiency, which may be related to the decreased content of abscisic acid. The content of SGs was significantly increased after 50 mg/L AOS treatment, which not only increased the contents of stevioside (STV) and rebaudioside A (Reb A) significantly, but some important minority glucosides, like Reb E, Reb D, and Reb M. The increased SGs contents were the combined effect of the higher expression of SGs biosynthesis related genes, including KAH, UGT74G1, UGT85C2, and UGT91D2. The geometry changes of stem induced by COS and AOS may help to increase the lodging resistance of stevia. Thus, COS and AOS can be used in the field planting of stevia to increase the yield of SGs for industrial purposes.
Assuntos
Diterpenos do Tipo Caurano , Stevia , Stevia/metabolismo , Biomassa , Glucosídeos/metabolismo , Diterpenos do Tipo Caurano/metabolismo , Sacarose/metabolismo , Folhas de Planta/metabolismo , Glicosídeos/metabolismoRESUMO
Sheath blight, caused by Rhizoctonia solani (R. solani), is one of the most serious diseases of rice. Extracellular polysaccharides (EPS) are complex polysaccharides secreted by microbes that have a pivotal role in the plant-microbe interaction. At present, many studies have been carried out on R. solani, but it is not very clear whether the EPS is secreted by R. solani exists. Therefore, we isolated and extracted the EPS from R. solani, two kinds of EPS (EW-I and ES-I) were obtained by DEAE-cellulose 52 and Sephacryl S-300HR column further purification, and their structures were characterized by FT-IR, GC-MS, and NMR analysis. The results showed that EW-I and ES-I had similar monosaccharide composition but different molar ratio, they were composed of fucose, arabinose, galactose, glucose, and mannose with a ratio of 7.49: 27.72: 2.98: 6.66: 55.15 and 3.81: 12.98: 6.15: 10.83: 66.23, and their backbone may be composed of â2)-α-Manp-(1â residues, beside ES-I was highly branched compared to EW-I. The exogenous application of EW-I and ES-I had no effect on the growth of R. solani AG1 IA itself, but their pretreatment of rice induced plant defense through activation of the salicylic acid pathway, resulting in enhanced resistance to sheath blight.
Assuntos
Oryza , Espectroscopia de Infravermelho com Transformada de Fourier , Doenças das Plantas , Polissacarídeos/farmacologiaRESUMO
Dibutyl phthalate (DBP) is an organic pollutant frequently detected in soil, and is a reproductive poison that harms animals both before and after birth and has mutagenic, teratogenic, and carcinogenic effects. DBP removal from farmland has been the subject of extensive research in recent years. Efficient DBP degrading bacterial strains were screened in the laboratory. GFP (Green fluorescent protein) labeled degradation strain GFP-DNB-S1 was analyzed for its activity and dynamics. Using sodium alginate (SA) and nano-hydroxyapatite (n-HAP) as carrier materials and CaCl2 as a cross-linking agent, the immobilized microbial agent n-HAP/SA + DNB-S1 was prepared by embedding cross-linking immobilization technology to study the remediation effect of DBP contaminated soil. The best formation effect of immobilized materials (n-HAP/SA) was found when the SA to n-HAP ratio was 3:2. When compared to single SA immobilized bacteria, n-HAP/SA immobilized bacteria improved the surface roughness and porosity of the microspheres. After 70 days, LED light revealed that the immobilized bacteria's GFP green fluorescent protein expression was stable. At 70 days, the initial DBP concentration of 500 mg â L-1 degraded at a rate of 69.9%. The degrading bacteria had no effect on DBP degradation before and after being labeled with GFP. The n-HAP/SA immobilized bacteria offered a better living environment for microorganisms due to their rougher surface and a greater number of pores. This protected the microorganisms and increased the efficiency of DBP degradation. When the concentration of DBP in contaminated soil was set to 20 mg â kg-1 and the n-HAP/SA + DNB-S1 immobilized bacterial agent was applied to the soil, the rate of DBP degradation was determined to be 93.34%. The degradation process followed First-order degradation kinetics, which improved the physical and chemical properties of the soil as well as its fertility.
Assuntos
Dibutilftalato , Poluentes do Solo , Dibutilftalato/metabolismo , Biodegradação Ambiental , Proteínas de Fluorescência Verde/metabolismo , Bactérias/metabolismo , Solo , Poluentes do Solo/metabolismoRESUMO
Heavy metal pollution is increasing, and rare earth elements (REE) play an important role in the environmental impact of heavy metals. Mixed heavy metal pollution is a major issue with complex effects. Despite substantial research on single heavy metal pollution, relatively few studies have focused on pollution from rare earth heavy metal composites. We studied the effects of different concentrations of Ce-Pb on the antioxidant activity in root tip cells and biomass of Chinese cabbage. We also used the integrated biomarker response (IBR) to evaluate the toxic effects of rare earth-heavy metal pollution on Chinese cabbage. We used programmed cell death (PCD) for the first time to reflect the toxicological effects of heavy metals and rare earths and studied the interaction between Ce and Pb in root tip cells in depth. Our results showed that Ce-Pb compound pollution can induce PCD in the root cells of Chinese cabbage, and the toxicity of compound pollutants is greater than that of single pollutants. Our analyses also provide the first evidence that Ce and Pb exert interaction effects in the cell. Ce induces Pb transfer in plant cells. The Pb content in the cell wall decreases from 58% to 45%. Additionally, Pb induced Ce valence changes. Ce (III) decreased from 50% to 43%, while Ce (IV) increased from 50% to 57%, directly resulting in PCD in the roots of Chinese cabbage. These findings improve our understanding of the harmful effects of compound pollution with rare earth metals and heavy metals on plants.
Assuntos
Brassica , Poluentes Ambientais , Metais Pesados , Metais Terras Raras , Poluentes do Solo , Chumbo/análise , Meristema , Poluentes do Solo/análise , Metais Pesados/análise , Metais Terras Raras/análise , Poluentes Ambientais/análise , Brassica/metabolismo , Apoptose , SoloRESUMO
With the continuous development of modern science, more and more attention is paid to the application of science and technology for agricultural production. First of all, this article analyzes the development trend of modern agriculture, and designs the system through an overall plan of four parts: automatic acquisition, terminal control, network transmission, and cloud platform and terminal application. Taking automatic irrigation system and temperature automatic control system as examples, design an automatic control algorithm based on fuzzy neural network, and obtain the best control strategy through fuzzy inference and neural network training. Then, this paper studies the reliability of "price setting" in agricultural income insurance. This article takes the main crops of a certain province as an example to verify and analyze the risks of farmers' income levels from the viewpoint of corn yield risks and price risks, and advocate the necessity and feasibility of agricultural income insurance. Finally, from the perspective of different participants, this article analyzes the impact of insurance premium subsidies on the enthusiasm of agricultural producers to participate in insurance, the impact of insurance companies on the improvement of insurance operation efficiency in the planting industry, and the stability of the government's promotion of agricultural production. It also influences to design a set of IoT automatic control system for intelligent agriculture. The system realizes the acquisition and remote control of agricultural data by establishing an Internet of Things cloud platform, and uses automatic control algorithms based on fuzzy neural networks to realize automatic water-saving irrigation, automatic temperature control, and other functions. Based on this research, the main actions of joining agricultural income insurance and the participation model based on the perspective of tripartite participants.
Assuntos
Agricultura , Seguro , Produtos Agrícolas , Humanos , Redes Neurais de Computação , Reprodutibilidade dos TestesRESUMO
Background: Cancer patients with POLE or POLD1 mutations may be excellent candidates for immune checkpoint inhibitors (ICIs) therapy and have favorable prognosis, but their potential in stomach adenocarcinoma (STAD) remains unknown. Therefore, the clinical significance of POLE and POLD1 mutations in STAD was evaluated. Methods: A summary of POLE/POLD1 mutations and clinical characteristics was performed on all 613 STAD samples, from which 360 samples were screened for analysis of the potential clinical relevance of POLE/POLD1 mutations to prognosis and immunotherapy. Results: The total frequency of both POLE and POLD1 mutations was 7.99% in STAD patients, correlating with an older age of onset and more frequently in the antrum anatomic subdivisions. Several genes that related to prognosis and immunotherapy also had high mutation frequencies in POLE/POLD1-mutant STADs. Furthermore, the STAD subgroup with POLE/POLD1 mutations had longer progression free survival (PFS) and overall survival (OS) in the subpopulation under 80. More importantly, STAD patients with POLE/POLD1 mutations exhibited adaptive immune resistance tumor microenvironment (TME) and deficient mismatch repair (dMMR) status, and possessed significantly higher PD-L1 expression level, higher tumor mutational load (TMB), higher microsatellite instability (MSI) percentage, and lower aneuploidy score, all of which may have potential implications for better ICIs treatment outcomes. Conclusions: POLE and POLD1 mutations are promising useful biomarkers to improve the clinical efficiency of practicing precision medicine in STAD patients, including as positive prognostic markers and predictive biomarkers of immunotherapy outcomes for STAD patients.
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OBJECTIVE: The clinical features of solitary pulmonary nodules (SPN) in breast cancer patients were retrospectively analyzed, and the clinical features of primary lung cancer (PLC) and metastatic pulmonary breast cancer (MBC) in breast cancer patients were compared, and the treatment plan, curative effect and influencing factors were analyzed. METHODS: The clinical data of 106 patients of SPN combined with breast cancer surgery in our hospital from January 2015 to June 2020 were analyzed. There were 65 patients of PLC and 41 patients of MBC. Record the characteristics of the primary breast cancer lesion in our patient, the interval between the initial diagnosis of breast cancer and the appearance of SPN, the previous treatment history of our patient, and the characteristics and surgical method of SPN. The survival status of all patients during the follow-up period was recorded. RESULTS: The onset age, interval, maximum nodule diameter, ER expression positive rate and radiotherapy history ratio of PLC patients were higher than those of MBC patients, and the lymph node positive rate and triple negative rate were lower than those of MBC patients (P < 0.05). Median survival was 51 months in patients with PLC and 37 months in patients with MBC. The 1, 3, and 5 year overall survival rates in patients with PLC were higher than those in patients with MBC (P < 0.05). Vascular tumor thrombus, SPN type and chemotherapy were all independent factors affecting the prognosis of patients with breast cancer combined with SPN (P < 0.05). CONCLUSION: PLC patients and MBC patients have significant differences in pathological characteristics, like the onset age, interval, maximum nodule diameter, ER expression positive rate, radiotherapy history ratio, the lymph node positive rate, and triple negative rate. Septum, vascular tumor thrombus, SPN type, and chemotherapy are all independent factors that affect the curative effect of breast cancer patients with SPN. Based on the nature of SPN, it can provide reference for clinicians to decide the treatment plan, improve patients' quality of life and prolong their survival time.
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Rebaudioside D (Reb D) and rebaudioside M (Reb M) are commercially important low/no-calorie natural sweeteners. However, they are present in a minor proportion of all steviol glycosides (SGs) in Stevia rebaudiana Bertoni (S. rebaudiana). Strain-dependent deviation in Reb D and Reb M biosynthesis is one key breach for breeding of S. rebaudiana, which has not been studied at the transcriptional level. Herein, five different S. rebaudiana varieties with distinct SGs contents, one cultivar having high stevioside content (HST), one cultivar having high Reb A content (HRA) and three cultivars having high Reb D and Reb M content (HDM1, HDM2, HDM3), were selected for RNA-seq analysis. In total, 131,655 de novo assembled unigenes were found in the RNA-seq data. According to Reb D and Reb M content divergence of S. rebaudiana accessions, 2186 differentially expressed genes (DEGs) were selected as potential genes related to Reb D and Reb M biosynthesis. Weighted Gene Co-expression Network Analysis (WGCNA) was used to explore the genes associated with the Reb D and Reb M biosynthesis. The unigenes from the positively associated turquoise module formed a layered co-expression network. There are 7 UDP-dependent glycosyltransferases (UGT) and 76 transcription factors (TFs) distributing at different regions which represented varying coherence of Reb D and Reb M biosynthesis. Particularly, two TFs having a strong correlation with two UGTs in the network were also discovered. The present study provided a comprehensive insight into networks for regulation of Reb D and Reb M contents in S. rebaudiana.
Assuntos
Diterpenos do Tipo Caurano , Stevia , Glicosídeos , Melhoramento Vegetal , Folhas de Planta , Stevia/genética , Transcriptoma , TrissacarídeosRESUMO
BACKGROUND: Application of chitin attracts much attention in the past decades as the second abundant polysaccharides in the world after cellulose. Chitin oligosaccharides (CTOS) and its deacetylated derivative chitosan oligosaccharides (COS) were shown great potentiality in agriculture by enhancing plant resistance to abiotic or biotic stresses, promoting plant growth and yield, improving fruits quality and storage, etc. Those applications have already served huge economic and social benefits for many years. However, the recognition mode and functional mechanism of CTOS and COS on plants have gradually revealed just in recent years. OBJECTIVE: Recognition pattern and functional mechanism of CTOS and COS in plant together with application status of COS in agricultural production will be well described in this review. By which we wish to promote further development and application of CTOS and COS-related products in the field.
Assuntos
Quitosana , Agricultura , Quitina , Humanos , Oligossacarídeos , PlantasRESUMO
RNA-binding proteins (RBPs) are pivotal participants in post-transcriptional gene regulation. They interact with RNA directly to perform several post-transcriptional RNA regulatory functions or direct metabolic processes. Despite the essential importance, the understanding of plant RBPs is elementary, which derives mainly from other kingdoms via bioinformatic extrapolation or mRNA-binding proteins captured through UV crosslinked method. Recently, orthogonal organic phase separation (OOPS) method for RBP identification has been used in mammals and Escherichia coli. And plentiful RBPs were enriched without molecular tagging or capture of polyadenylated RNA in an unbiased way. In our study, OOPS was conducted on Arabidopsis and 468 RBPs were discovered including 244 putative RBPs. There were 17 peroxidases in 232 RBPs with enzymatic activities. In addition, Arabidopsis thaliana-Pst DC3000-chitinpentaose interaction system was chosen to explore whether OOPS can be used to dig specific RBPs under special physiological conditions. Eighty-four differential RBPs in this system were found and some of them involved in reactive oxygen species (ROS) metabolic pathway. These results showed OOPS can be applied to plants successfully and would be a useful method to identify RBPomes and specific RBPs.
Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Moléculas com Motivos Associados a Patógenos/metabolismo , Proteoma/metabolismo , Proteínas de Ligação a RNA/metabolismo , Redes e Vias Metabólicas/fisiologia , Oligossacarídeos/metabolismo , Peroxidases/metabolismo , RNA/metabolismo , Espécies Reativas de Oxigênio/metabolismoRESUMO
Oligogalacturonides (OGAs) are a biologically active carbohydrate derived from homogalacturonan, a major element of cell wall pectin. OGAs induced resistance and mechanism were assessed in Arabidopsis thaliana-Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) interaction. The effective resistance was mainly observed at 25 mg/L OGAs with reduced disease index, bacterial multiplication, higher transcript level of salicylic acid (SA) pathway related genes (PR1, PR2, PR5) and jasmonic acid (JA) pathway related genes (PDF1.2, VSP2) as well as SA, JA content and production of reactive oxygen species (ROS), nitric oxide (NO). In SA (NahG, sid2) and JA (jar1) deficient mutants, disease severity indicated that both SA and JA pathways are necessary for Arabidopsis response to Pst DC3000. OGAs triggered less resistance to Pst DC3000 in JA-deficient mutant, and SA-deficient mutants signifying that SA and JA play redundant roles in OGAs induced resistance. Therefore, these evidences further reveal the signaling pathways of OGAs resistance, which is conducive to its application in agriculture to protect plants from diseases.
Assuntos
Arabidopsis/microbiologia , Arabidopsis/fisiologia , Ciclopentanos/metabolismo , Resistência à Doença , Redes e Vias Metabólicas , Oligossacarídeos/metabolismo , Oxilipinas/metabolismo , Doenças das Plantas/microbiologia , Ácido Salicílico/metabolismo , Fenômenos Químicos , Regulação da Expressão Gênica de Plantas , Monossacarídeos/química , Óxido Nítrico/metabolismo , Oligossacarídeos/química , Fenótipo , Folhas de Planta/metabolismo , Folhas de Planta/microbiologia , Pseudomonas syringae , Espécies Reativas de Oxigênio/metabolismo , Análise EspectralRESUMO
Roles of protein N-glycosylation in chitosan oligosaccharide (COS) induced resistance were investigated in the present study. Results demonstrated that N-glycosylation deficient Arabidopsis mutants (stt3a and ManI) were more susceptible against Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) than wild type (WT) plants. Surprisingly, in stt3a and ManI, COS-induced resistance to Pst DC3000 was mostly intact, and the up-regulation effect on SA- and JA-mediated signalling pathways also similar like WT. Nucleotide sugars accumulation and N-glycosylation related genes expression were differently regulated after COS treatment. Global glycomics analysis quantified 157 N-glycan isomers, and 56.7, 50.3 and 47.1 % of them were significantly changed in COS, mock + Pst, and COS + Pst treated plants, respectively. Moreover, COS pretreatment could reverse the effect of Pst DC3000 on many N-glycans, suggesting that COS regulates protein N-glycosylation via a non-canonical pattern compared with plant defense, which may contribute to its obvious disease control effect when N-glycosylation impairment occurs.
Assuntos
Arabidopsis/imunologia , Quitosana/química , Resistência à Doença/imunologia , Oligossacarídeos/farmacologia , Doenças das Plantas/imunologia , Polissacarídeos/metabolismo , Pseudomonas syringae/fisiologia , Arabidopsis/efeitos dos fármacos , Arabidopsis/metabolismo , Arabidopsis/microbiologia , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Resistência à Doença/efeitos dos fármacos , Regulação da Expressão Gênica de Plantas , Glicosilação , Solanum lycopersicum/microbiologia , Doenças das Plantas/microbiologiaRESUMO
Breast cancer is a heterogeneous disease highly diverse in different subtypes, including hormone receptor positive and hormone receptor negative subtypes with variable malignancy, therapy regimen, and different prognosis. In this study, we develop a hormone receptor-specific mRNA-miRNA-lncRNA ceRNA network to identify whether several RNAs play fundamental roles in development and metastasis of breast cancer. To understand the association of ceRNA expression profiles in different breast cancer subgroups, the expression profiles and clinical information of 428 HR+/Her-2- breast cancer samples and 113 triple negative breast cancer samples were downloaded from The Cancer Genome Atlas database (TCGA). We comprehensively integrated and compared expression profiles of mRNAs, miRNAs, and lncRNAs between the two subgroups mentioned. Aberrantly expressed hormone receptor specific RNAs were identified, whereas lncRNA-miRNA interactions predicted by miRcode and miRNA-targeted mRNA interactions were validated by miRTarBase, Targetscan, and miRDB database. In this study, mRNA-miRNA-lncRNA ceRNA network was constructed that consisted of 44 miRNA-lncRNA interaction pairs and 2 miRNA-mRNA interaction pairs, and visualized by Cytoscape software. Prognostic markers of HR-specific subtype of breast cancer associated with overall survival were identified by Kaplan-Meier survival analysis. Finally, SFRP1, AC006449.1, and MUC2 were novel clinical predictors that may also provide a new therapeutic target in the future.
Assuntos
Neoplasias da Mama/genética , Neoplasias da Mama/mortalidade , MicroRNAs/genética , RNA Longo não Codificante/genética , Idoso , Biomarcadores Tumorais/genética , Neoplasias da Mama/patologia , Feminino , Regulação Neoplásica da Expressão Gênica , Redes Reguladoras de Genes , Humanos , Peptídeos e Proteínas de Sinalização Intercelular/genética , Estimativa de Kaplan-Meier , Proteínas de Membrana/genética , Pessoa de Meia-Idade , Mucina-2/genética , Prognóstico , RNA Mensageiro/genética , Receptor ErbB-2/metabolismo , Receptores de Esteroides/metabolismo , Neoplasias de Mama Triplo Negativas/genética , Neoplasias de Mama Triplo Negativas/mortalidade , Neoplasias de Mama Triplo Negativas/patologiaRESUMO
BACKGROUND: Her-2 positive subtype breast cancer is characterized as Her-2 gene amplification with poor survival and increased invasiveness accounting for 20-30% of invasive infiltrated breast cancer. A lncRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network is constructed to detect Her-2 specific RNAs in the development and progression of HER-2 positive breast cancer which may overcoming the anti-HER-2 therapy resistance of breast cancer cells. METHODS: One thousand one hundred and nine breast cancer samples obtained from The Cancer Genome Atlas (TCGA) database were classified into two cohorts including ER+/PR+ (n=461) and ER-/PR- breast cancer (n=152). Differently expressed mRNAs, lncRNAs and miRNAs were screened in ER+/PR+ and ER-/PR- breast cancer cohorts, respectively. lncRNA-miRNA interactions were preformed to predicted and verified by miRcode. miRNA-mRNA interactions were selected to predict targeted mRNAs of miRNAs by miRanda, Targetscan and miRTarBase. RESULTS: lncRNA-miRNA-mRNA ceRNA network was constructed by retained lncRNAs, miRNAs and mRNAs. Fifteen DEmiRNAs, 129 DElncRNAs and 269 DEmRNAs were retained in ER+/PR+ cohort after intersection with DEmiRNAs, DElncRNAs and DEmRNAs between breast cancer and normal tissues. Six hundred and ninety-three DEmRNAs, 25 DEmiRNAs and 364 DElncRNAs were retained in ER-/PR- cohort. ceRNA network in ER+/PR+ breast cancer cohort was constructed of the interactions of 4 DElncRNA-DEmiRNA pairs and 2 DEmiRNA-DEmRNA pairs included 4 DElncRNAs, 1 DEmiRNAs, and 2 DEmRNAs. ceRNA network in ER-/PR- breast cancer cohort was constructed of the interactions of 24 DElncRNA-DEmiRNA pairs and 1 DEmiRNA-DEmRNA pairs included 19 DElncRNAs, 4 DEmiRNAs, and 1 DEmRNA. MIR7-3HG- hsa-mir-204-NTRK2 axis was identified in both ER+/PR+ and ER-/PR- cohort in our study. CONCLUSIONS: Based on the ceRNA hypothesis, a potential Her-2 related regulatory ceRNA networks are constructed which may provide novel insights into the mechanism underlying the biological processes of Her-2 positive breast cancer.
RESUMO
Chitosan oligosaccharides (COS) worked effectively in multiple plant-pathogen interactions as plant immunity regulator, however, due to the complexity of the COS-induced immune signaling network, the topic requires further investigation. In the present study, quantitative analysis of proteins was performed to investigate the underlying mechanism of COS induced resistance to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) in Arabidopsis thaliana. 4303 proteins were successfully quantified, 186, 217 and 207 proteins were differently regulated in mock + Pst, COS, and COS + Pst treated plants, respectively, compared with mock plants. From detailed functional and hierarchical clustering analysis, a priming effect of COS on plant immune system by pre-regulated the key proteins related to signaling transduction, defense response, cell wall biosynthesis and modification, plant growth and development, gene transcription and translation, which confers enhanced resistance when Pst DC3000 infection in Arabidopsis. Moreover, RACK1B which has the potential to be the key kinase receptor for COS signals was found out by protein-protein interaction network analysis of COS responsive proteins. In conclusion, COS treatment enable plant to fine-tuning its defense mechanisms for a more rapid and stronger response to future pathogen attacks, which obviously enhances plants defensive capacity that makes COS worked effectively in multiple plant-pathogen interactions.